Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,451,280 C→T P75S (CCG→TCG)  ytfR → galactofuranose ABC transporter putative ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,451,2800CT99.0% 311.3 / NA 95P75S (CCG→TCG) ytfRgalactofuranose ABC transporter putative ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (47/47);  minor base G (0/1);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

ACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATT  >  NC_000913/4451036‑4451525
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     
aCGCTTTACACCGGGGGAAACCCTGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGACAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCCCCATCTGGCTGGAAGGCCAGGCTATATCATCGaaa                                                                                                                                                                                                                                                  >  1:651771/1‑250 (MQ=255)
aCGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGaaaa                                                                                                                                                                                                                                                 <  2:351508/251‑1 (MQ=255)
     ttACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATAc                                                                                                                                                                                                                                             <  2:498224/250‑1 (MQ=255)
     ttACAACGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTACGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATAc                                                                                                                                                                                                                                             <  2:402249/250‑1 (MQ=255)
         cccGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGccc                                                                                                                                                                                                                                        <  2:23351/250‑1 (MQ=255)
         cccGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGccc                                                                                                                                                                                                                                        <  2:599682/250‑1 (MQ=255)
          ccGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGcccc                                                                                                                                                                                                                                       >  1:597795/1‑250 (MQ=255)
          ccGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCa                                                                                                                                                                                                                                       <  1:501140/251‑1 (MQ=255)
                gAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGca                                                                                                                                                                                                                                 <  1:450176/251‑1 (MQ=255)
                    ccATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGcaacaa                                                                                                                                                                                                                             <  1:686031/251‑1 (MQ=255)
                          cgaccgacCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGc                                                                                                                                                                                                                       <  2:682169/251‑1 (MQ=255)
                               gaccAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCTCAACAACTCGGCATTgg                                                                                                                                                                                                                  <  1:202040/251‑1 (MQ=255)
                                accAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGTTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGc                                                                                                                                                                                                                 <  2:475444/251‑1 (MQ=255)
                                accAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGc                                                                                                                                                                                                                 >  2:100589/1‑251 (MQ=255)
                                 ccAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTCGTTATAAGCCTGCGCCGTGGCGAAATAATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAAAGCTACTAAAAGCATTAAATGGAGTATACCACGCCGATAGCGGCACCATCAGGCTGGAAGGACAGGCTATCTCATCGAAAAATACCGCCAAAGCGCAACAACTCGGAATTGGc                                                                                                                                                                                                                 >  1:72151/1‑250 (MQ=255)
                                   aaCACCAGGCGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCCAAAACTCGGCATTGGCAcc                                                                                                                                                                                                              >  2:658939/1‑251 (MQ=255)
                                             gaTCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAgg                                                                                                                                                                                                    <  1:302056/251‑1 (MQ=255)
                                                cctccGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAATGCGTTAGACAACGTTGATTTCAGCCTTCGCCGTGGCGAAATCATGGCGCTTCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATTCCACGCCGAACGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACGCGGCATTGGCACCGTCTAGCAGGAAg                                                                                                                                                                                                 <  2:658217/251‑1 (MQ=255)
                                                 ctccGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAACTCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTCTCAGGAAgg                                                                                                                                                                                                >  2:516283/1‑250 (MQ=255)
                                                   ccGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCAGCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGt                                                                                                                                                                                                <  2:458365/249‑1 (MQ=255)
                                                             ggATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTAcc                                                                                                                                                                                    <  2:523733/251‑1 (MQ=255)
                                                               aTTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAACCCGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATAGCGGCCCCATCTGGCTGGAAGGCCAGGCTATATCATCGAAAAATACCGCCCACGCGCCACAACTCGGCCTTGGCCACGTCTATCAGGAAGTCCACCTGCTAccc                                                                                                                                                                                   >  2:686378/1‑250 (MQ=255)
                                                                      aaaTTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAATGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATgtc                                                                                                                                                                           <  1:140770/251‑1 (MQ=255)
                                                                           tttCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCGCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATgtcggtc                                                                                                                                                                       <  1:110894/250‑1 (MQ=255)
                                                                           tttCCCCGGCGGCAAAGCGTTAGACCACGTTGATTTCAGGCTGCGCCGTGGCGAAATCGTGGCGCTGCGCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAACGTCTATCAGGAAGTCAACCTGCTACCCAATATgtcggtc                                                                                                                                                                       <  2:168378/250‑1 (MQ=255)
                                                                            ttCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGc                                                                                                                                                                     >  1:664623/1‑251 (MQ=255)
                                                                            ttCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGCGGCGAAATCATGGCGCTGATCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCCTTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGc                                                                                                                                                                     >  1:662148/1‑251 (MQ=255)
                                                                               cccGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTTGCACCGTCTATCAGGGAGTCAACCTGCTACCCAATATGTCGGTCGCTgg                                                                                                                                                                  >  2:376129/1‑250 (MQ=255)
                                                                                ccGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGAt                                                                                                                                                                 >  1:65476/1‑251 (MQ=255)
                                                                                 cGGCGTCAAAGCGTTAGACAAAGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGCAAATCAACGCCAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGGCTATCAGGAAGTCAACCTGCTACCCAATCTGTCGGTCGCTgtt                                                                                                                                                                 >  2:62644/1‑248 (MQ=255)
                                                                                       cAAAGCGTTAGACAACGTTGCTTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATTCCGCCCACGCGCAACAACTCGGCCTTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGCTAATCttt                                                                                                                                                          >  2:462702/1‑249 (MQ=255)
                                                                                             gTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAACATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTAGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCCATATGTCGGTCGCTGATAATCTATTtat                                                                                                                                                      >  2:272494/1‑249 (MQ=255)
                                                                                             gTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTtat                                                                                                                                                      >  1:285876/1‑249 (MQ=255)
                                                                                             gTTAAACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCAAGCCGATCGCGGCAACATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAACGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTtat                                                                                                                                                      >  1:174282/1‑249 (MQ=255)
                                                                                               tAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGc                                                                                                                                                  >  1:180712/1‑251 (MQ=255)
                                                                                                 gACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCcg                                                                                                                                                >  1:279748/1‑251 (MQ=255)
                                                                                                   cAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCcgcg                                                                                                                                              >  2:98449/1‑251 (MQ=255)
                                                                                                    aaCGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCcgcg                                                                                                                                              >  1:278128/1‑250 (MQ=255)
                                                                                                          gATTTCAGCCTGCGCAGTGACGCAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCCTTAACTGGTGTATACCACGACGAACGCGGCACCATCTGGCTGGAAGGCCCGGCTATCTCATAGAAAAATACCGCCCCCGCGCAACCACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATCATCTATTTATAGGCCGCGAACCCa                                                                                                                                        >  2:305741/1‑250 (MQ=255)
                                                                                                            tttCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGGGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACACCTAGGGATTGGCACCGTCTATCAGGCAGTCAACCTTCTACCCCATATGTCGGTCGCTTATAATCTATTTATAGGCCGCGAACCCAAAc                                                                                                                                     >  2:224664/1‑251 (MQ=255)
                                                                                                             ttCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGGCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTAGAGGCCGCGAACCCAAAc                                                                                                                                     <  2:259050/250‑1 (MQ=255)
                                                                                                             ttCAGACTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGATCCCAAAc                                                                                                                                     <  1:381946/250‑1 (MQ=255)
                                                                                                               cAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCt                                                                                                                                  <  2:231629/251‑1 (MQ=255)
                                                                                                                aGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGACCATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCCTCTGGCTGGAAGGCCAGGCTATCTCTTCGCAAAATACCGCCCACGCCCACCAACCAGGCCTTGGCCCCGTCTATCAGGAAGTCAACCTGCTACCCAATCTGACGGGCGCTGATAATCTATTTATAGGCCGCGAAACCCAACGCtt                                                                                                                                 >  1:732241/1‑251 (MQ=255)
                                                                                                                 gCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCCCGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCCATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTc                                                                                                                                >  1:152996/1‑251 (MQ=255)
                                                                                                                  ccTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGACAATCACCGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCg                                                                                                                               >  2:364524/1‑251 (MQ=255)
                                                                                                                    tGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCCTTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGc                                                                                                                             >  2:506671/1‑251 (MQ=255)
                                                                                                                         cGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCATGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCt                                                                                                                        <  1:516283/251‑1 (MQ=255)
                                                                                                                                aaaTCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCGGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCTCTAACCCAAACGCTTCGGCCTTCTAcgccgc                                                                                                                 <  2:384991/251‑1 (MQ=255)
                                                                                                                                     aTGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAaga                                                                                                            <  2:288963/251‑1 (MQ=255)
                                                                                                                                       ggCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCTCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAagag                                                                                                           <  2:540174/250‑1 (MQ=255)
                                                                                                                                        gcgcTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGAt                                                                                                         <  1:17183/251‑1 (MQ=255)
                                                                                                                                          gctgctCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCCTTAACTGGTGTAAACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATACGGAAGTCAACCTGCTACCCCATATGTCGGTCGCTGATAATCTATTTATAGGACGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATgg                                                                                                       >  2:321278/1‑251 (MQ=255)
                                                                                                                                             gctCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGaaa                                                                                                    <  2:209125/251‑1 (MQ=255)
                                                                                                                                                cGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAg                                                                                                  <  2:152025/250‑1 (MQ=255)
                                                                                                                                                 ggTGAAAACGGGGCGGGAAAATCAACGCTATACAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAAGCCAGGCTATCTCATCGAAAAAGACCGCCCACGCGCATCAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTTCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGTCCTTCTACGCCGCAAGGAGATGTaaaatct                                                                                                <  2:121453/251‑4 (MQ=255)
                                                                                                                                                     aaaaCGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGGATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGcc                                                                                            <  1:396601/251‑1 (MQ=255)
                                                                                                                                                        aCGGGGCGGGAAACTCAACGCTAATCAAAGCATTAACTGGTGTATACCCCGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGccccc                                                                                         >  2:507561/1‑248 (MQ=255)
                                                                                                                                                        aCGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCAcc                                                                                         <  1:151881/251‑1 (MQ=255)
                                                                                                                                                             gCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGACAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCCTCTATCAGGAAGTCAACCTGCTACCCACTATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAACCGCTTCGGCCTTCTCCGCCGCAAAGAGATGGAAACGCGCGCCACCGacct                                                                                    >  2:442519/1‑248 (MQ=255)
                                                                                                                                                               gggAAAATCAACGCTAATAAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCCTTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTg                                                                                   >  2:76549/1‑250 (MQ=255)
                                                                                                                                                                       cAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCTCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATc                                                                           <  1:591727/250‑1 (MQ=255)
                                                                                                                                                                              aatcacagagttAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATAGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACAGTCTATCAGGAAGTCAACCTGCTACACAATATGTCGGGCGCTGATAATCTATTTATGGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGtt                                                                   >  2:377742/11‑251 (MQ=255)
                                                                                                                                                                              aaTCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGtt                                                                   >  1:498267/1‑251 (MQ=255)
                                                                                                                                                                               aTCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGttt                                                                  <  1:348333/251‑1 (MQ=255)
                                                                                                                                                                                tCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTtc                                                                 >  1:336830/1‑251 (MQ=255)
                                                                                                                                                                                   aaGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTcc                                                              >  2:329748/1‑251 (MQ=255)
                                                                                                                                                                                     gCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCt                                                            <  2:285876/251‑1 (MQ=255)
                                                                                                                                                                                       aTTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCg                                                          >  1:451318/1‑251 (MQ=255)
                                                                                                                                                                                          aaCTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGAct                                                       <  2:16295/251‑2 (MQ=255)
                                                                                                                                                                                              ggTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTgc                                                    <  1:664250/250‑1 (MQ=255)
                                                                                                                                                                                              ggTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTg                                                     <  2:152996/249‑1 (MQ=255)
                                                                                                                                                                                               gtgtATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTGCCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTgcgc                                                  <  1:493806/251‑1 (MQ=255)
                                                                                                                                                                                                 gtATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACGCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAATGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGCCGTGcgcg                                                 <  1:697799/250‑1 (MQ=255)
                                                                                                                                                                                                         cGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCa                                        <  1:506671/251‑1 (MQ=255)
                                                                                                                                                                                                            cGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAAcc                                     <  1:62644/251‑1 (MQ=255)
                                                                                                                                                                                                             gATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCACCAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTCCCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGACCTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCg                                    >  2:335860/1‑251 (MQ=255)
                                                                                                                                                                                                             gATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCg                                    <  1:21536/251‑1 (MQ=255)
                                                                                                                                                                                                              aTCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGc                                   >  1:69564/1‑251 (MQ=255)
                                                                                                                                                                                                                 gcgGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCttt                                >  1:466307/1‑251 (MQ=255)
                                                                                                                                                                                                                  cgGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGCCGTGCGCGAACCGCTCAACCGCtttt                               >  2:221916/1‑251 (MQ=255)
                                                                                                                                                                                                                  cgGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGACAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCtttt                               >  1:303415/1‑251 (MQ=255)
                                                                                                                                                                                                                      aCCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGt                           >  1:312209/1‑251 (MQ=255)
                                                                                                                                                                                                                       ccagctggctggAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAg                            <  2:499239/245‑1 (MQ=255)
                                                                                                                                                                                                                       ccATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGTCACCGTCTATCAGGAAATCAACCTGCTACCCAATATgtcg                                                                                                                                                                          <  2:43745/107‑1 (MQ=255)
                                                                                                                                                                                                                       ccATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATgtcg                                                                                                                                                                          >  1:43745/1‑107 (MQ=255)
                                                                                                                                                                                                                            tggctggAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGCTGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGAt                     >  1:517217/1‑251 (MQ=255)
                                                                                                                                                                                                                             ggctggAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGAt                     <  1:318145/250‑1 (MQ=255)
                                                                                                                                                                                                                             ggctggAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATg                    <  1:272494/251‑1 (MQ=255)
                                                                                                                                                                                                                                tggAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGCAGTCAACCTGCTACCCAATATGTCGGTCGCTGCTAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATgc                   >  2:738436/1‑249 (MQ=255)
                                                                                                                                                                                                                                  gAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATgcagc                >  1:516195/1‑250 (MQ=255)
                                                                                                                                                                                                                                  gAAGGCCAGGCTATCTCATCGAAAAATACCGACCACGCGCACCAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATGTATAGGACGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGAGCAACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACAGCGCAACCGCTTTTCAGTCGCGATgcaga                >  2:407548/1‑249 (MQ=255)
                                                                                                                                                                                                                                     ggCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAt            <  2:651771/251‑1 (MQ=255)
                                                                                                                                                                                                                                      gCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAtc           <  1:100589/251‑1 (MQ=255)
                                                                                                                                                                                                                                               tctcATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTAtt  >  2:173832/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     
ACGCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATT  >  NC_000913/4451036‑4451525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑