BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,380,469	C→T	E17K (GAA→AAA)	frdB ←	fumarate reductase iron‑sulfur protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,380,469	0	C	T	100.0%	442.3 / NA	124	E17K (GAA→AAA)	frdB	fumarate reductase iron‑sulfur protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (72/52); total (72/52)

Only 100 of 124 total aligned reads displayed.

CGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGAC  >  NC_000913/4380224‑4380715

cGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTGTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCggg                                                                                                                                                                                                                                                    <  2:49096/250‑1 (MQ=255)

 gggAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGtt                                                                                                                                                                                                                                                  <  1:139791/251‑1 (MQ=255)

  ggAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGAAAGGTTTTACATGCCAGTTTAGGCGCGTTGTTAACCCTCATGCCGCACGAACCACAAATAGCCCTCCGCCAGGACCAGCGGTAGATCAGGTCCGGAGCCAGGTA‑GTCATTGAAGTAGCCCAGCGCATCCAGTCCTGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGCGCGGTATCGACTTTCGGGttc                                                                                                                                                                                                                                                 >  1:110298/1‑250 (MQ=255)

       ttAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAgcg                                                                                                                                                                                                                                            >  1:73102/1‑251 (MQ=255)

          gCTAACGCTTCAACCTTCATACCGGCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTAGCACGTTGTTAACCATCATGCCGCAGGCACCCAAAATCGCCATCCGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCGGGTT‑GTTTTTGATGTAGCCCAGCGCATCCAGTAATGCGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGGGCGGTACCGGCTTTCGGGTTATAGCGcac                                                                                                                                                                                                                                         >  2:713824/1‑251 (MQ=255)

              aCGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCaccacc                                                                                                                                                                                                                                     <  1:64412/251‑1 (MQ=255)

               cGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCt                                                                                                                                                                                                                                    <  1:425481/251‑1 (MQ=255)

                 cTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACTACCTcc                                                                                                                                                                                                                                  >  1:629653/1‑250 (MQ=255)

                    cAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCCTCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAAtt                                                                                                                                                                                                                               >  2:504221/1‑251 (MQ=255)

                           cATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACCAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GACTTTGATGTAGACCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCCTAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGt                                                                                                                                                                                                                        >  1:89936/1‑251 (MQ=255)

                           cATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGt                                                                                                                                                                                                                        >  1:280609/1‑251 (MQ=255)

                               ccGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCCGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCAACTCAATTTTCAGGttttt                                                                                                                                                                                                                    >  1:729074/1‑251 (MQ=255)

                                 gTCGGCGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGAAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACAACCTCAATTTTCAGGTTTTTc                                                                                                                                                                                                                   >  2:268390/1‑250 (MQ=255)

                                   cGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCAt                                                                                                                                                                                                                 <  1:382525/250‑1 (MQ=255)

                                    ggTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCAtct                                                                                                                                                                                                               >  1:157592/1‑251 (MQ=255)

                                      tgtAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCAtctc                                                                                                                                                                                                              >  2:579237/1‑250 (MQ=255)

                                       gtAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCAGCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGGTTTTCATCTCAg                                                                                                                                                                                                            <  2:326429/251‑1 (MQ=255)

                                        tAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGc                                                                                                                                                                                                           >  2:535062/1‑251 (MQ=255)

                                        tAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGGAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGGTATAGCGCACCACCTCACTTTTCAGGTTTTTCATCTCAGc                                                                                                                                                                                                           >  2:580027/1‑251 (MQ=255)

                                            cACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATA‑GGCAATTCATAGAATGCGCCATGCGGTGCGGTATCGACTTTCGGGGTATAGCCCACCCCCTCAATTTTCAGGTTTTTTAtcctagcccttc                                                                                                                                                                                                      >  2:151060/1‑241 (MQ=255)

                                                cAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGcc                                                                                                                                                                                                   >  2:15100/1‑251 (MQ=255)

                                                  ggAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGcctt                                                                                                                                                                                                 <  1:158364/251‑1 (MQ=255)

                                                   gAAGGTTTTACATGCCAGTTTTGGGACGGTGGTAACCATCATGCCGCAGGAACCACAAAGCGCCATACGGCAGGACCAGCGGTAGCGCAGGGCCGGTGCCAGGTT‑GTCTGTGATGTAGACCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGcctac                                                                                                                                                                                                <  2:240247/251‑3 (MQ=255)

                                                    aaGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGccttct                                                                                                                                                                                               >  1:234389/1‑251 (MQ=255)

                                                       gTTTTACATGCCAGTTTTGGCACGTTGTTCACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGccttctcct                                                                                                                                                                                            >  1:602642/1‑251 (MQ=255)

                                                        ttttACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCCGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCACTTTTCAGGTTTTTCATCTCAGCCATTCGccttctcctt                                                                                                                                                                                           >  1:386704/1‑251 (MQ=255)

                                                         tttACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGccttctccttc                                                                                                                                                                                          >  1:727784/1‑251 (MQ=255)

                                                                gCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTgg                                                                                                                                                                                   <  1:401343/251‑1 (MQ=255)

                                                                   aGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGgctg                                                                                                                                                                                <  1:329370/251‑1 (MQ=255)

                                                                   aGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGgctg                                                                                                                                                                                >  2:168589/1‑251 (MQ=255)

                                                                   aGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGAAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGGGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCCGGTTTTTCATCTCCGCCATTCGCCTTCTCCTTCTTATTAgctg                                                                                                                                                                                >  2:4416/1‑251 (MQ=255)

                                                                         ggCACGTTGTTAACCATCATGCCTCAGGATCCACAACTCGCCATGCGGCATGACCAGCTTTATCGCAGGTCCGGTGCCAGGTTTGT‑TTTGATGTAGCCCAGCGCATCCAGTAATGAGGTACTTGCTTCATAAGGCACTTCATAGAATGCGCCATGCGGTGCGGTATCGACGTTCGGGTTATAGCGCACCACCTCAAGTTTCAGGTTTTTCATCTCAGCCATTCTCCTTCTCCTTCTTATTGGCTGCTTcc                                                                                                                                                                           <  1:62750/250‑1 (MQ=255)

                                                                            aCGTTGTTAACCATCATTCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCATCCATTCGCCTTCTCCTTCTTATTGGCTTCTTCCGCCt                                                                                                                                                                       >  1:280422/1‑251 (MQ=255)

                                                                                 gttAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCg                                                                                                                                                                  <  2:174203/251‑1 (MQ=255)

                                                                                   tAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCgg                                                                                                                                                                 <  2:335822/250‑1 (MQ=255)

                                                                                        atcatGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGGGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACACCCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCAt                                                                                                                                                           >  1:331963/1‑251 (MQ=255)

                                                                                          catGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATcc                                                                                                                                                         <  1:501809/251‑1 (MQ=255)

                                                                                             gCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCCCTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCt                                                                                                                                                      <  2:629653/251‑1 (MQ=255)

                                                                                              ccGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCtt                                                                                                                                                     <  2:8588/251‑1 (MQ=255)

                                                                                               cGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTc                                                                                                                                                    >  1:658411/1‑251 (MQ=255)

                                                                                                gCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGCGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGAGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCg                                                                                                                                                   >  1:720361/1‑251 (MQ=255)

                                                                                                 cAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGc                                                                                                                                                  <  2:163601/251‑1 (MQ=255)

                                                                                                    gAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCAc                                                                                                                                               >  2:480080/1‑251 (MQ=255)

                                                                                                        cacaAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTa                                                                                                                                           <  1:583150/251‑1 (MQ=255)

                                                                                                         acaAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATA‑GGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTaaa                                                                                                                                         <  1:151060/251‑1 (MQ=255)

                                                                                                          caAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTaaa                                                                                                                                         <  2:260103/251‑1 (MQ=255)

                                                                                                           aaaTCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAAc                                                                                                                                        >  2:42973/1‑251 (MQ=255)

                                                                                                           aaaTCGCCATACGGCAGGACAAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAAc                                                                                                                                        >  2:283413/1‑251 (MQ=255)

                                                                                                            aaTCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTGCGCCACCGTAAAcg                                                                                                                                       <  1:578774/251‑1 (MQ=255)

                                                                                                                 ccATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGttt                                                                                                                                  <  2:566595/251‑1 (MQ=255)

                                                                                                                                   ggTAGCGCAGGTCCGGTGCCAGGTG‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGGTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCgtagt                                                                                                                <  2:710048/251‑1 (MQ=255)

                                                                                                                                    gTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTTCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCgtagta                                                                                                               <  1:379388/251‑1 (MQ=255)

                                                                                                                                    gTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCgtagt                                                                                                                >  1:297617/1‑250 (MQ=255)

                                                                                                                                          cAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTc                                                                                                         <  2:516713/251‑1 (MQ=255)

                                                                                                                                           aGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCa                                                                                                        <  1:599878/251‑1 (MQ=255)

                                                                                                                                                 ggTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGc                                                                                                  >  1:235319/1‑251 (MQ=255)

                                                                                                                                                  gTGCCAGGAT‑GACTTTGATGTAGCCCAGCGCACCCACTAATGAGGCAGTTGCGTCATAAGGCACTTCATCGATTGCGCTATGCGGTGCGGTACCGACTTTCGGGTTATAGCGCACAACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCt                                                                                                 >  2:381737/1‑251 (MQ=255)

                                                                                                                                                    gCCAGGTT‑GTCTTTGCTGTAGCCCCGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGt                                                                                               >  2:80416/1‑251 (MQ=255)

                                                                                                                                                     ccAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGt                                                                                               >  2:511136/1‑250 (MQ=255)

                                                                                                                                                      taGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTAc                                                                                             >  1:412225/2‑251 (MQ=255)

                                                                                                                                                        ggTT‑GTATTTGAAGTAGCACAGCGCATCCAGTAATGAGGTAGTTACGTCATAATGCACTTCATAGAATGCCCTATTCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCCGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCGTCCGCTTCGCCACCGTAAACGCGTTTAGCTGTCGGCAGCGGAGTAAACTTCACGACGCTGTACTc                                                                                           >  2:682281/1‑251 (MQ=255)

                                                                                                                                                                 ttGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCg                                                                                     >  1:66550/1‑249 (MQ=255)

                                                                                                                                                                   gATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTc                                                                                 >  1:540040/1‑251 (MQ=255)

                                                                                                                                                                   gATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTGCTCCTTCGTATTGGCTGCTTCCGCCTTATCGGCTTCATCCGCTTCGCCACCGTAAACGCGTTTATCTGGCGGCAGCGTAGTAATCTTCACGGCGCTGTACTCCAGGCGAGt                                                                                  <  1:285603/250‑1 (MQ=255)

                                                                                                                                                                    aTGTAGCCCAGCGCATCCAGTAATGCGGTAGTTGCGTCATAAGGCACTTCCTAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTCGCTGGCGGCCGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCg                                                                                >  2:653927/1‑251 (MQ=255)

                                                                                                                                                                        aGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCTACTTTCGGGTTATAGCGCACCACCTCAATTTGCAGGTTTTTCATCTCAGCCATTCGCCTTTTCCTTCTTATTGGCTGCTTCCGCCGTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTGAGCTGGCGGCAGCGTAGTAAGCTTCACGTCGCTGTACTCCAGGCGAGTCGTGc                                                                             <  2:636517/250‑1 (MQ=255)

                                                                                                                                                                        aGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGcc                                                                            >  1:95032/1‑251 (MQ=255)

                                                                                                                                                                          cccAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACGCCAGGCGAGTCGTGCCAt                                                                          <  2:148287/251‑1 (MQ=255)

                                                                                                                                                                            cAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATc                                                                         >  2:578824/1‑250 (MQ=255)

                                                                                                                                                                                   tCCAGTACTGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTCGCTGGCGGCCGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATcgc                                                                 >  1:293322/1‑251 (MQ=255)

                                                                                                                                                                                       gTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCGTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGaa                                                             <  2:212843/251‑1 (MQ=255)

                                                                                                                                                                                         aaTGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAgg                                                           >  1:198962/1‑251 (MQ=255)

                                                                                                                                                                                          aTGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAACCGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGc                                                          >  1:499287/1‑251 (MQ=255)

                                                                                                                                                                                                tAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGCgggg                                                    >  2:561384/1‑250 (MQ=255)

                                                                                                                                                                                                  gTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACATCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGgtgg                                                  >  1:442524/1‑250 (MQ=255)

                                                                                                                                                                                                    tGCGTCATACGGCACTTCATAAAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGCGGgtgtgt                                                >  2:610143/1‑251 (MQ=255)

                                                                                                                                                                                                     gCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGgtgtgt                                                <  2:73102/250‑1 (MQ=255)

                                                                                                                                                                                                     gCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGtt                                               <  2:540040/251‑1 (MQ=255)

                                                                                                                                                                                                     gCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTGTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCATGCGAGGCGTGCTATCAGCAGCGCGGAAGGCGAGGttgtgt                                                <  2:702651/250‑1 (MQ=255)

                                                                                                                                                                                                          aTAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAgg                                          >  1:232843/1‑251 (MQ=255)

                                                                                                                                                                                                          aTAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAgg                                          >  1:137565/1‑251 (MQ=255)

                                                                                                                                                                                                          aTAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTGTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGGGTGTGTGAgg                                          <  1:579237/251‑1 (MQ=255)

                                                                                                                                                                                                            aaGGCACTTCATAGAATGCGCTATGCGGGGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGGGCCAGCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGa                                         <  2:239063/250‑1 (MQ=255)

                                                                                                                                                                                                             aGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGa                                         >  2:596579/1‑249 (MQ=255)

                                                                                                                                                                                                              ggCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGCGGAAGt                                      >  1:135022/1‑251 (MQ=255)

                                                                                                                                                                                                               gCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCAACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTAGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGtt                                     >  1:142804/1‑251 (MQ=255)

                                                                                                                                                                                                                    tCATAGAATGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGAc                                  <  1:23605/249‑1 (MQ=255)

                                                                                                                                                                                                                           aTGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCTCCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACg                         <  1:170608/251‑1 (MQ=255)

                                                                                                                                                                                                                           aTGCGCTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACg                         <  2:380719/251‑1 (MQ=255)

                                                                                                                                                                                                                              cgcTATGCGGTGCGGTATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTc                      >  1:154611/1‑251 (MQ=255)

                                                                                                                                                                                                                                   tgcggtgaggTATAGACTTTCGGGGTATAGCGCACCACCTCCATTTTCAGGTAATTCATCTCAGCCATTCGACTTCTCCTTCTTCTTGGCTGCTTCCGCCTTATCGGCTGCCTCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTAGATGTACTACAGGCGAGTCGTGCCATCAGCATCGCGGAAGGAGAGGGTGTGTTTGAGGCAGTTGAcgtcagactcatttaagc                 >  2:729899/1‑237 (MQ=255)

                                                                                                                                                                                                                                    gcggtgcggtATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCAATCGCCGTCTCCTTCTTATTGGCTGCCTCCGCCTTATCGGCTGCATCCGCTTCGCAACCGTAAACGCGTTTAGCTGGCTGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCa                <  1:481577/251‑1 (MQ=255)

                                                                                                                                                                                                                                          cggtATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGCGGAAGTTGACGTCGTCACGCTCGGTGCACCCTTc          >  2:40138/1‑251 (MQ=255)

                                                                                                                                                                                                                                          cggtATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTc          <  2:331963/251‑1 (MQ=255)

                                                                                                                                                                                                                                           ggtATCGACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCg         >  1:206369/1‑251 (MQ=255)

                                                                                                                                                                                                                                            gtATCGCCTTTCGGGTTATAACGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCATTCTTATTGGCTGCTTCCGCCTTATCGCCTGCATCCGATTCGCCACCGTAAACGCGTTTAGCTGGCTGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGTAAGGCGAGGGTGTGTGTGCGGAAGTTGACGTCGTCACGCTCGGGGCAACCTTCGt        >  2:462831/1‑251 (MQ=255)

                                                                                                                                                                                                                                                 gACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTcc      >  1:166145/1‑248 (MQ=255)

                                                                                                                                                                                                                                                 gACTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAgc   >  2:387730/1‑250 (MQ=255)

                                                                                                                                                                                                                                                  aCTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGAc  >  2:456604/1‑251 (MQ=255)

                                                                                                                                                                                                                                                  aCTTTCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGAc  >  1:709376/1‑251 (MQ=255)

CGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTT‑GTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGGCAGCGTAGTAATCTTCACGTCGCTGTACTCCAGGCGAGTCGTGCCATCAGCATCGCGGAAGGCGAGGGTGTGTTTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGAC  >  NC_000913/4380224‑4380715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`