Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,366,668	C→T	A36T (GCC→ACC)	dcuA ←	C4‑dicarboxylate transporter DcuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,366,668	0	C	T	100.0%	146.0 / NA	43	A36T (GCC→ACC)	dcuA	C4‑dicarboxylate transporter DcuA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/21);  total (22/21)

TCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTA  >  NC_000913/4366424‑4366912                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     ccagggaaaacgggcaggggccccAGAGAGATGTTGCGAGTACCAGCAAAGATATAGAGGAAATAGGTCGGGATGGGGGGGAGGATGGTGATGTATTGGGGTTTGG‑GGCGCAGCAGCTTTTCTTTTTAAT‑AACCAAGATAGCCCA‑GCCCGCCAGCACCCGGAATGGAAAAAATGGCGGCGAAAGCCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAAc                                                                                                                                                                                                                                                <  1:552172/229‑1 (MQ=255)   atcgagaccgggcagttccgacAGAGAGCTTTTCCCAGTCCCATCAAAAATAGTCAGGAAATAGTTCACGATGGGTGCGAGGTTCTTTATTTTTTTCGGTTTCC‑GGCGCCGCACCTTTTCTGTCTGATAAACGA‑AATA‑TCCAATCCCGCAACCAACTTACGTGGCAAAATTAGGGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCa                                                                                                                                                                                                                                              <  1:263910/230‑1 (MQ=255)     cGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCACAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGCTCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGc                                                                                                                                                                                                                                            >  2:52707/1‑251 (MQ=255)        tAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGCTCGGTGCGAGGCTCGTGATGTATTTCGGGTTAC‑GGCGCCGCAGCTTTTCTGTCTGATGAACCA‑GATAGTACA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGCCATCGAACGGGATGTTACCGGGTTTAACGACAATAGCGGTAGGAACCAGCAcc                                                                                                                                                                                                                                         >  1:720656/1‑251 (MQ=255)            tGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCAccccc                                                                                                                                                                                                                                      >  2:197457/1‑250 (MQ=255)                      cccAGAGAGATGTTGCCAGTACCAAAAAAGATAGTGAGAAAATGGGCCAGGGCGGGGGGGAGTTTCGTTAT‑TTTTTGGGGTTTCCGGCGCACCAGTTTTTCTTTTTGATGACCCA‑AATAGTCCA‑GCCCGCCACCACCCTGCATGGAAAAAAAGGCGGCAAAAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGc                                                                                                                                                                                                                           <  1:236649/250‑1 (MQ=255)                                tGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAACACCAAt                                                                                                                                                                                                                  >  1:721341/1‑250 (MQ=255)                                        gTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTcc                                                                                                                                                                                                         >  1:212490/1‑251 (MQ=255)                                                             aaaTAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTTGCGCCCaaga                                                                                                                                                                                     >  2:383500/1‑250 (MQ=255)                                                                           gggggcggggtgtgtgattttttgtgggTCAC‑GGCGCAACAGCTTTTCTGTCGGATAAACAA‑GATAGTACA‑ACCCCCCAGAAACCTGCAGGGCAAAAATAGGGGGGAAAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCaaaa                                                                                                                                                                      <  1:558082/226‑1 (MQ=255)                                                                             gTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACt                                                                                                                                                                    >  2:263435/1‑251 (MQ=255)                                                                                       cGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAAc                                                                                                                                                                     >  1:114553/1‑240 (MQ=255)                                                                                         tGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCCATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCt                                                                                                                                                        >  1:433412/1‑251 (MQ=255)                                                                                                                           ttgtctgATAAACAA‑GATAGCCCA‑GCCCGCCAGAAACCTGCAGGGAAAAAATGGCGGCAATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTa                                                                                                                        <  1:197457/249‑1 (MQ=255)                                                                                                                             gtctgATGAAGCA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGATTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTaaa                                                                                                                      >  2:666407/1‑249 (MQ=255)                                                                                                                                  atgaAACA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGACAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACATATTCCCCCCAATCTGGCGCCCCAGAAGATCGCCAGCATAACTATGATGAGTTCTACAACTAGCATATTTGCCTTCCTTGTTTTTTAAAAAGttt                                                                                                                >  2:115161/1‑250 (MQ=255)                                                                                                                                            aTAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGACGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATAtt                                                                                                           >  2:211912/1‑247 (MQ=255)                                                                                                                                                  gcaacccgcaagaaacgtgaagggaaGAAATGGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTGTTTTAACAAGTTGATATTAGATTGTTa                                                                                                  <  2:433412/226‑1 (MQ=255)                                                                                                                                                      aCCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTAtttt                                                                                              >  2:660413/1‑251 (MQ=255)                                                                                                                                                                 ccTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGc                                                                                   >  2:157589/1‑251 (MQ=255)                                                                                                                                                                    gaggggagaaatggtgggtgATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCGACAACTATCATATTAGCCTGCCTTGTTTTGTAACAAGTGGATATTAGATTGTTAATTTTAAGTGACTGCt                                                                                  <  2:283769/230‑1 (MQ=255)                                                                                                                                                                          cAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGa                                                                           >  2:466631/1‑250 (MQ=255)                                                                                                                                                                            aaaaTAGGGGCAATACCCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGaaaa                                                                        <  1:108230/250‑1 (MQ=255)                                                                                                                                                                               agggcggcgATGACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCCCCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTGTTAACAAGATGAGATTAGATTGTTATTTTTAAGTTTCTGCGCACAAGAAAAAAg                                                                     <  2:707028/248‑1 (MQ=255)                                                                                                                                                                                         tAACCGCCATGATAATGGAGATGACCTCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACACCTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATc                                                           >  2:717849/1‑251 (MQ=255)                                                                                                                                                                                         aacccGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATc                                                           <  1:38546/248‑1 (MQ=255)                                                                                                                                                                                           cccGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTg                                                         <  2:360351/250‑1 (MQ=255)                                                                                                                                                                                             cGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAAGAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCACAATCTGGCGCCCAAGAAGAACGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGTACGTCAACTGAc                                                       <  1:235709/251‑1 (MQ=255)                                                                                                                                                                                                    aTAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCtt                                                >  2:674236/1‑251 (MQ=255)                                                                                                                                                                                                       aTGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCttttt                                             <  2:212490/251‑1 (MQ=255)                                                                                                                                                                                                       aTGGAGAGGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCTCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCATCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCttttt                                             <  2:562123/251‑1 (MQ=255)                                                                                                                                                                                                        tGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGcc                                                                                                                                                                                                                              >  2:99311/1‑73 (MQ=255)                                                                                                                                                                                                        tGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGcc                                                                                                                                                                                                                              <  1:99311/73‑1 (MQ=255)                                                                                                                                                                                                           agaTGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTAtt                                         >  2:284065/1‑251 (MQ=255)                                                                                                                                                                                                                    cGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTAcc                                >  1:365855/1‑251 (MQ=255)                                                                                                                                                                                                                    cGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTAcc                                <  1:68674/251‑1 (MQ=255)                                                                                                                                                                                                                          ggATGTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTAc                          <  1:383500/251‑1 (MQ=255)                                                                                                                                                                                                                              gTTACCGGGTTTAACGCCAATAGCGGTAAGAACCAGCCCCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGAGTAGTTCGACAACTAGCATAGTAGCCTTCCTTGGTTGGTAACAAGTTGATATTAGATTGTTATTTTTAAGTGACTGCTCACAAGAAAAAAGGCACGTCAGCTGACGTGCCTGTTTTATTTGTACTACCCGGTACGAt                       <  2:720656/250‑1 (MQ=255)                                                                                                                                                                                                                               ttACCGGGTTTAACCCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAACACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAATTATGATGATTTCTACAACTAGTATATTAGCCTGCCTTGTTTTTTAACAAGTTGATATTAGATTGTGAGTTTTAAGTTACTGCTCACCAGAAAAAAGGTACGTCATCTGACGGGTCTTTGTTATTTGTACTTCCCTGTACGAtt                      <  2:495302/250‑1 (MQ=255)                                                                                                                                                                                                                                      gTTTAACGCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGtgcg              <  2:103142/251‑4 (MQ=255)                                                                                                                                                                                                                                             gCCAATAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcat       >  1:636271/1‑251 (MQ=255)                                                                                                                                                                                                                                                aaTAGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTtcatc      >  1:145968/1‑249 (MQ=255)                                                                                                                                                                                                                                                   aGCGGTAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGta  <  2:210300/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     TCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATAGGTCACGATCGGTGCGAGGATCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCA‑GATAGTCCA‑GACCGCCAGCAACCTGCATGGCAGAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGCTCACAAGAAAAAAGGCACGTCATCTGACGTGCCTTTTTTATTTGTACTACCCTGTACGATTACTGTTCGCTTTCATCAGTA  >  NC_000913/4366424‑4366912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑