Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,349,259	C→T	intergenic (‑515/+56)	dcuB ← / ← dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,349,259	0	C	T	100.0%	160.9 / NA	45	intergenic (‑515/+56)	dcuB/dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/13);  total (32/13)

ATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCTCGTTGGCTAGCTCGTCGGTTGAA  >  NC_000913/4349013‑4349498                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                aTTGATGTTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGAAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAg                                                                                                                                                                                                                                              <  1:475133/250‑1 (MQ=255)                            tctcAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAattat                                                                                                                                                                                                                  >  1:230848/1‑251 (MQ=255)                               cAGGGCAGCTTTCATTAGGATTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTAttac                                                                                                                                                                                                               >  2:496601/1‑251 (MQ=255)                                    cAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTAttacttac                                                                                                                                                                                                           >  1:154297/1‑250 (MQ=255)                                       aTGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATCTTTGATCTGGCACAAGTTTTACTGATGCAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTAttacttac                                                                                                                                                                                                           >  2:397909/1‑247 (MQ=255)                                           cATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAAcc                                                                                                                                                                                                   >  1:539355/1‑251 (MQ=255)                                           cATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGGTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAAGTATTACTTACAGATAAcc                                                                                                                                                                                                   <  2:14124/251‑1 (MQ=255)                                            aTTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAAcc                                                                                                                                                                                                   >  1:147608/1‑250 (MQ=255)                                                      taGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGtt                                                                                                                                                                                        >  2:97568/1‑251 (MQ=255)                                                       aGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTa                                                                                                                                                                                       <  2:519080/251‑1 (MQ=255)                                                            aaTCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTgg                                                                                                                                                                                   <  1:539151/250‑1 (MQ=255)                                                                 gATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAat                                                                                                                                                                               <  2:554734/249‑1 (MQ=255)                                                                                   tAAAAATCCACATATTGCGGGTGACATAATAGTTAATTACCTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGAAATATTGTTTCagtagtgagt                                                                                                                                                             >  2:449899/1‑249 (MQ=255)                                                                                              aTAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTg                                                                                                                                                <  1:355029/251‑1 (MQ=255)                                                                                                              aataGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATacggtaacggta                                                                                                                                 >  1:374343/1‑250 (MQ=255)                                                                                                               ataGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATAcggtaacggtaa                                                                                                                                >  2:638465/1‑250 (MQ=255)                                                                                                                taGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAAc                                                                                                                              >  1:641439/1‑251 (MQ=255)                                                                                                                     aattaaCTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGAc                                                                                                                         <  2:17897/251‑1 (MQ=255)                                                                                                                        taaCTTTTGTTAACGTTTTGCAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATACTTATCTTTGCTCTGGCACATGTTTTACTGATGACGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGcc                                                                                                                      >  2:350793/1‑251 (MQ=255)                                                                                                                             tttGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTg                                                                                                                 >  1:167170/1‑251 (MQ=255)                                                                                                                               tGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGAc                                                                                                               >  1:263220/1‑251 (MQ=255)                                                                                                                                         ttGAAATTATAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGCGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATgg                                                                                                     >  2:596779/1‑251 (MQ=255)                                                                                                                                          tGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATgg                                                                                                     >  1:181922/1‑250 (MQ=255)                                                                                                                                           gAAATTAAAAACACCGTTCACCTGCAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTCGTGCTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGAt                                                                                                   >  2:198296/1‑251 (MQ=255)                                                                                                                                              aTTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACt                                                                                                <  2:154297/251‑1 (MQ=255)                                                                                                                                                tAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTgg                                                                                              >  1:632771/1‑251 (MQ=255)                                                                                                                                                tAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTgg                                                                                              >  2:595224/1‑251 (MQ=255)                                                                                                                                                  aaaaCACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTg                                                                                            >  1:104725/1‑251 (MQ=255)                                                                                                                                                    aaCACCGTGCAACTGAAGAAATATTAATTTTTAGCGCTGTAGGAGGGCTTATTATATATGATCTGGCACAAGTTTTAATGATGAAGGATGTAACTTGTGTCAGGGGTATATTGCATTCCGGTAATAATTATTACTTACAGATAACCAGCGATCAGATATAGGCAATATTGTTTCAGTAGAGAGTAGTGGTCTGCCTGAATACGGTAACGGTAATCTGGACGCACCGTGACGCCATAATGGATACTAGTgc                                                                                           >  2:426919/1‑249 (MQ=255)                                                                                                                                                      cacCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAAc                                                                                         >  1:112764/1‑250 (MQ=255)                                                                                                                                                                cggAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGGCATAATGGATACTGGTGATCAAGATGTGGCa                                                                              <  1:625051/249‑1 (MQ=255)                                                                                                                                                                  gAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATGTTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTGTCAGTATTGATTAGTGTTCTGCCTGGATACGGTAACGGTAAACTGGACGACCCGTGACGCCATTATGGAGACTGTTGAACAAGATTTGGCa                                                                              <  2:419900/249‑1 (MQ=255)                                                                                                                                                                  gAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCa                                                                              <  1:411313/249‑1 (MQ=255)                                                                                                                                                                               tttttAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagcc                                                                   >  2:612125/1‑247 (MQ=255)                                                                                                                                                                                    aGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAgg                                                           >  2:634942/1‑250 (MQ=255)                                                                                                                                                                                     gCGATGATGGAGGGATAATTATATTTGATCTGGCACACGTTTTAATGATGAAGGATGTAACTTGTGTCAGGGGCATTTTGCATTAAGGTAATCATTATTACTTACAGATAACCAGCGATCCGTTATTGGCACTATTGTTTCAGTAGTGCGTAGTGTTCTGCATGAATACGGTAACGGTAAACTGGACGCACCGTGACGCCATAATGGATCCTGGTGAACACGATGTGGCAGTTGACCAGCCAGATGAGGt                                                          >  2:712818/1‑250 (MQ=255)                                                                                                                                                                                        atgatgGAGGGATAATTATCTTTGATCTGGCACACGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATACTTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCTTAATGGATACTGGTGAACAAGATGCGGAAGTTGAccagccagctggggaattt                                                      >  2:640105/1‑240 (MQ=255)                                                                                                                                                                                                                  cTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTa                             >  1:587297/1‑250 (MQ=255)                                                                                                                                                                                                                  atGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACAAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTa                             >  1:587318/2‑250 (MQ=255)                                                                                                                                                                                                                     gCACAAGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATTGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCt                         <  1:348930/251‑1 (MQ=255)                                                                                                                                                                                                                          aGTTTTACTGATGAAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCTCGTTg                    <  2:104725/251‑1 (MQ=255)                                                                                                                                                                                                                                   gatgaAGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCCCGTTGGCtcacccg           >  2:183122/1‑245 (MQ=255)                                                                                                                                                                                                                                        aGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCTCGTTGGCTAGCTCGTCGGt      >  2:186629/1‑251 (MQ=255)                                                                                                                                                                                                                                        aGGATGTAACTTGTGTCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCTCGTTGGCTAGCTCGTCGGt      >  1:678978/1‑251 (MQ=255)                                                                                                                                                                                                                                             gTAACTTGTGTCAGGGGTCTTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCTCGTTGGCTAGCTCGTCGGTTGaa  >  1:557862/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                ATTGATATTTATCGGTCTATTTTTTGTTCTCAGGGCAGATGTCATTAGGTTTATAGATTAATCTGATCTACCCATTTGTGGGTAAAAATACACATAATGCGGGTGACATAATAGTTAATTAACTTTTGTTAGCGTTTTGAAATTAAAAACACCGTTCACCTGAAGAGATATTAATTTTTAGCGATGATGGAGGGATAATTATATTTGATCTGGCACAAGTTTTACTGATGAAGGATGTAACTTGTGCCAGGGGTATTTTGCATTACGGTAATAATTATTACTTACAGATAACCAGCGATCCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATTTACGGCAGGAAACACGCGAAATGTTAACCTCGTTGGCTAGCTCGTCGGTTGAA  >  NC_000913/4349013‑4349498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑