Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,278,488	C→T	P4S (CCA→TCA)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,278,488	0	C	T	100.0%	194.6 / NA	56	P4S (CCA→TCA)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/27);  total (29/27)

CGTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGC  >  NC_000913/4278244‑4278729                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    cGTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAg                                                                                                                                                                                                                                               <  2:543055/251‑1 (MQ=255)        aCAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTAc                                                                                                                                                                                                                                        <  1:529045/251‑1 (MQ=255)          aaTAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACcg                                                                                                                                                                                                                                      >  1:186856/1‑251 (MQ=255)                agagAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGtt                                                                                                                                                                                                                                <  2:470198/251‑1 (MQ=255)                 gagaAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGttt                                                                                                                                                                                                                               >  2:407168/1‑250 (MQ=255)                    aaaaaaGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CAcc                                                                                                                                                                                                                           >  1:68221/1‑250 (MQ=255)                    aaaaaaGCAGCAAACTTCGCCTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACATCATCAGCGCGTACcggcggattcat                                                                                                                                                                                                                            >  1:611679/1‑245 (MQ=255)                         agcagcAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGtttcctcgtcc                                                                                                                                                                                                                       >  2:223568/1‑242 (MQ=255)                               aaaCTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTAGCGGCGGTT‑CCCTC‑GATGccctgg                                                                                                                                                                                                               >  2:670762/1‑247 (MQ=255)                                          gAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑TACTccatgaccgggtttaacatttc                                                                                                                                                                                                     >  2:210420/1‑230 (MQ=255)                                            aaaaGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTcaca                                                                                                                                                                                                  <  1:211664/251‑1 (MQ=255)                                                        gATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCAc                                                                                                                                                                                      <  1:223568/251‑1 (MQ=255)                                                                 aTAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCTCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCa                                                                                                                                                                             <  1:207876/251‑1 (MQ=255)                                                                    aTCGTTTGCTTTTTTTACCACCCGTTTTGGATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTgg                                                                                                                                                                                                                <  2:362753/213‑1 (MQ=255)                                                                           gCTTTTTTTACCCCCCGTTTTGTATGCGCGGATCTAAACGTTTGCTTTTGTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCCCAGCGTGGAAGCACTTTCCGTCAGGAAGTGGt                                                                                                                                                                    <  1:228748/250‑1 (MQ=255)                                                                           gCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGtt                                                                                                                                                                   <  1:407168/251‑1 (MQ=255)                                                                                 tttACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑TACTC‑CACGCCCGGTTTAAAATTTTACAACGTGGAAGCACTGTCCGTCCGGGAGTGGGTGcc                                                                                                                                                                >  1:52135/1‑248 (MQ=255)                                                                                 tttACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGcc                                                                                                                                                                <  2:52135/248‑1 (MQ=255)                                                                                            ttttGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCCGGTTTAAAATTTCACAACCTGGAAGCACTGTCCCCCAGGAAAGGGTTGCCGGGTTaacaaccat                                                                                                                                                  >  2:579868/1‑248 (MQ=255)                                                                                                 tATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CCCTC‑GACGCCCGGTTTAAAATTTTACAACGTGGGAGCACTGTCCGTCAGGAAGGGGTTGCCGGGGTaacaaaggttcttg                                                                                                                                             >  1:385564/1‑242 (MQ=255)                                                                                                   tGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CCCTC‑GACCCCCGGTTTAAAATTTCACAACGTGGGAGCACTGGCCGTCAGGAAGTGGGTGCCGGGTTAACAACGTTTCTGGCg                                                                                                                                           >  1:131833/1‑251 (MQ=255)                                                                                                      gcgGAGCTAAACGATTGATTTTTTGCGACGCAGCAAATTGCCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑TCCTC‑GCCGCCTGGTTTAAAATTTTACAACGTGGGAGCACTGTCCGTCAGGGAGGGGGTGCCGGGGTTACAAACTTTTTGGCGATg                                                                                                                                        >  2:724530/1‑251 (MQ=255)                                                                                                        ggAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGGTTAAAATTTTACAACGGGGAAGCACTGTCCGTCAGGAAGTGGGTGGCGGGGTAACAACGTTTTTGGCGGTggg                                                                                                                                      >  1:390702/1‑250 (MQ=255)                                                                                                         gAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTc                                                                                                                                     <  1:670762/251‑1 (MQ=255)                                                                                                         gAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTc                                                                                                                                     <  1:223934/251‑1 (MQ=255)                                                                                                          aGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCt                                                                                                                                    <  1:12058/251‑1 (MQ=255)                                                                                                             tAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGGTTAAAATTTTACAAAGTGGAAGCACTGTCCGTCAGGGAGGGGTTGGCGGGTTaacaaaggttttggggaggggcttcc                                                                                                                                 >  1:673596/1‑230 (MQ=255)                                                                                                                aCGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGCAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATATTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTCA‑TC‑GACACCTGGTTTTAATTTTTACACCGCGGAAACACAGTCCGTCAGGGAGTGATTGCCGGGGTTACAAAGCTTTTGGCGATGGGCTACACgg                                                                                                                              >  1:721546/1‑251 (MQ=255)                                                                                                                 cGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGt                                                                                                                             <  2:373374/251‑1 (MQ=255)                                                                                                                      gCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑TACTC‑GACGCCTGGGTTAAAATTTTACAACGTGGAAGCACCGTCCGCCAGGAAGAGGTTGCCGGGGTAACAACGATTCTAGGGATGGTCTCCTCGGTCCtcg                                                                                                                        >  1:466478/1‑251 (MQ=255)                                                                                                                      gCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GCCGCCCGGGTTTAAATTTCCCAACGTGGGAACACTGTCCGTCCGGAAGTGGTTTCCGGGTTAACAACGTTTTTGGGGGTGGTCTACTCGGTCAtcg                                                                                                                        >  1:431361/1‑251 (MQ=255)                                                                                                                        ttttttGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGAGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAACTTCACAACGGGGAAGCACTGTCCGTCAGGCAGTGGTTGCCGGGTTTAAAAAGGTTCTGGCGGTGGTCttatcgggctacggt                                                                                                                      >  1:448233/1‑237 (MQ=255)                                                                                                                              gCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTcc                                                                                                                 <  1:547210/250‑1 (MQ=255)                                                                                                                              gCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTGTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTcc                                                                                                                 <  2:385564/250‑1 (MQ=255)                                                                                                                                gACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACTTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCTGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAgg                                                                                                              <  1:306954/251‑1 (MQ=255)                                                                                                                                gACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTAACAAAATGGGAGCACTGTCCGCCAGGAAGTGGTTGCCGGGGTAACAAAGGTTCTTGCGGTGGGCTACTCTGTCATCATCGtttccgg                                                                                                              >  1:169132/1‑246 (MQ=255)                                                                                                                                       caAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTg                                                                                                       <  2:390702/251‑1 (MQ=255)                                                                                                                                               cGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTACACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGCT‑AACTCTGAC‑CCTCGTTTACAATTTCACAACGGTGAGGCCCTGTCCGTCAGGAACTCGTTGCCGGGCTAATAACGTTTCTGGCGGTGGGCTAATCGGTCATCCTAGTTTCCGGTATGTTggggcacgg                                                                                               >  2:311099/1‑245 (MQ=255)                                                                                                                                                            cAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTc                                                                                                                                <  1:230670/205‑1 (MQ=255)                                                                                                                                                            cAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAAAGTGGAAGCACTGTCCGTCAGGAAGTGGGTGCCGGGTTTACAACGGTTCTTGCGATGGTCTACTCGGGCATCGTCGTTCCAGGGCTGTTTGGGAAAGAAGGCTTccccccc                                                                                  >  1:631836/1‑247 (MQ=255)                                                                                                                                                               gAAGATAACGTTTCGCTGGCAGGGGATGGTCCGCCACGCAGCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCGTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCg                                                                               <  1:724530/251‑1 (MQ=255)                                                                                                                                                                        gttccGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGAGGGTCTACTCGGTCAGCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTc                                                                      <  2:44982/247‑1 (MQ=255)                                                                                                                                                                              cTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGcgcg                                                                                                                                                                                                                                           <  2:526730/82‑1 (MQ=255)                                                                                                                                                                              cTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGcgcg                                                                                                                                                                                                                                           >  1:526730/1‑82 (MQ=255)                                                                                                                                                                                       gATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTg                                                       <  1:405683/251‑1 (MQ=255)                                                                                                                                                                                        aTTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACAC‑GACGCCTGGTTTAAAATTTCACAACGTGGGAGCACTGTTCGGCAAGAAGTGGGTGCCGGGTTTACAAAGTTTTTGGCGAGGGGCTACTCGGTAATCGTCGTTCCAGGGATGTTGGGGAAAGCAGGCTTCCCCACTGCGGCGGGTTTTGTTGCAACCTGTCTgg                                                      >  2:692578/1‑251 (MQ=255)                                                                                                                                                                                                       tCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTTACAACGTGGGAACACTGTCCGTCAGGAAGTGGTTTCCGGGTTTACAACGGTTCTGGCGATGGGCTTCTCGGTCATCGTCGTTCCAGGGATGTTGGGGAAAGCAGGCTTCCCGCCTGCGGCCGTTTTCGTTGCAACCTGTCTGGTTGCCGGGCTCgggt                                       >  1:257438/1‑249 (MQ=255)                                                                                                                                                                                                          tGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CAATC‑GACGCCTGGGTTAAAATTTCACAAAGTGGGAGCACTGTCCGCCAGGAAGTGGGTGCCGGGGTAACCACGTTTTTGGCGATGGTCTACTCGGTCATCGTCGTTCCCGGTATGTTGGGTAAAGCAGGCTTCCCCCCTGCGGCAGGTTTTGTTGCCAACTGTCTGGTTTCCGGGCTCgggtttt                                    >  1:655508/1‑246 (MQ=255)                                                                                                                                                                                                                    tAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGGGGAAGCACTGTCCGTCAGGAAGTGGGTGCCGGGGTAACAACGGTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTTAAACAGGCTTTCCGCCTGCGGCCGGTTTCGTTGCAACCCGTTTGGTTGGCGGGCCCGGGTCTATCGTGGTggg                          >  2:8254/1‑251 (MQ=255)                                                                                                                                                                                                                       cctctCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGTTTTAAAATTTCACAGCGTGTAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCt                       <  1:210420/250‑1 (MQ=255)                                                                                                                                                                                                                        ctctCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGGGGGTTCCGGGGTAACAAAGTTTCTTGCGTTGGTCTACTCGGGCATCGTCGTTCCAGGGATGTTGGGGAAAGCAGGCTTCCCCCCTGCGGCCGTTTTTTTTTCCACCCGTCTGGGTGTCGGGCTCGGTTCttttgtggtggggctg                      >  1:86726/1‑236 (MQ=255)                                                                                                                                                                                                                            cAGGGGATGTTTGCTTATTTCTACTTCACCAGCGCTTACCGGCGGTT‑CACTC‑GACGCCGGGTTTAAAATTTCACAACGTGGAGGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTggt                  <  2:197121/251‑2 (MQ=255)                                                                                                                                                                                                                                   tGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑TACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGGAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGGTGGTCTACTCGGGCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTTGTTGCCGGGCTCGGGTCTATCGGGATGGGGCTGTGGGGTAATCt           >  1:3671/1‑251 (MQ=255)                                                                                                                                                                                                                                       ttCTTATGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTGGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGcc        <  2:131833/250‑1 (MQ=255)                                                                                                                                                                                                                                            aTGTCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAAATTCACAACGCGGGAGCACTGTCCGTCAGGGAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCCTCGTCGTTTCAGGGATGTTGGGGAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAAACTGTCTGGTTGCCGGGCTCGGTTTTATCGTTGTGGGGTTGGGGGGTAATCTGCCGTTggg  >  1:337895/1‑250 (MQ=255)                                                                                                                                                                                                                                               tCTACGTCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGt                                                          <  2:641034/192‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    CGTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTT‑CACTC‑GACGCCTGGTTTAAAATTTCACAACGTGGAAGCACTGTCCGTCAGGAAGTGGTTGCCGGGTTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCTGGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGC  >  NC_000913/4278244‑4278729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑