Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,269,449	C→T	C12C (TGC→TGT)	aphA →	acid phosphatase/phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,269,449	0	C	T	100.0%	106.9 / NA	30	C12C (TGC→TGT)	aphA	acid phosphatase/phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/12);  total (18/12)

TACATCGAGTAATAAACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAA  >  NC_000913/4269217‑4269687                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               tACATCGAGTAATAAACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTaa                                                                                                                                                                                                                                <  1:398120/249‑1 (MQ=255)        aGTAATAAACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATACTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCg                                                                                                                                                                                                                       >  1:491948/1‑251 (MQ=255)                         ataCAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATGTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTc                                                                                                                                                                                                       <  2:545221/250‑1 (MQ=255)                                     aCAGGACCGTATTTGCTGGGTTAAATCTAATCATCCTGCTTTTCATCACCAAAACCGCCGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCg                                                                                                                                                                                          >  2:110802/1‑251 (MQ=255)                                                        gTTAAATATAATCATCCTGCTTTTCATCACAACAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATCGTCAGATGGTTTTTGCAACAAATCTCACCATAAAACGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACg                                                                                                                                                                       >  2:276049/1‑251 (MQ=255)                                                                    catcCTGCTTTTCATCACAAAAACCGCAGATAATCCCTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCtt                                                                                                                                                            <  2:444661/250‑1 (MQ=255)                                                                            ttttCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAAc                                                                                                                                                     >  2:25398/1‑249 (MQ=255)                                                                             tttCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACa                                                                                                                                                    <  1:409577/249‑1 (MQ=255)                                                                               tcatcaCAACAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGcc                                                                                                                                                >  2:508639/1‑250 (MQ=255)                                                                                  ccacaAAAACCGCAGATAAACCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCATCGCCGCTTATCCCTGGGGCTAACGTTGCCAGGCTTGCTGAACAGGCGccc                                                                                                                                             <  2:257414/250‑1 (MQ=255)                                                                                   cacaAAACCCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCCCAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCAccc                                                                                                                                             >  2:450420/1‑250 (MQ=255)                                                                                                      ccttccttTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGc                                                                                                                         >  2:631251/1‑251 (MQ=255)                                                                                                                cccGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGaa                                                                                                               <  1:27375/251‑1 (MQ=255)                                                                                                                          ggCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTc                                                                                                      <  1:25398/250‑1 (MQ=255)                                                                                                                                      aGATGGTTTTTGCAACACATCTCACAATAAACAGTTTCACCATACTGACTATTTAGCGACAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGc                                                                                         >  2:381924/1‑251 (MQ=255)                                                                                                                                           gTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATg                                                                                    >  2:402230/1‑251 (MQ=255)                                                                                                                                           ctggttGCAACACATCTCACAGTAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCTCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTTTTGCCCTGGCCTCATCTCCTTCACCGCTTACCCCTGAGACTAACGTTGCCAGGCTTGCTGACCAGGCACCCATTCATTGGGTTTCGGTCGCACACATTGAAAATAGCCTCGCactgcttcctccaatg                                                                                    >  2:402214/5‑236 (MQ=255)                                                                                                                                                 gCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTg                                                                              <  2:181716/251‑1 (MQ=255)                                                                                                                                                          ctcACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGGTTGat                                                                     >  2:535509/1‑251 (MQ=255)                                                                                                                                                                       gTTTCAACATACTGCCTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACgg                                                        >  1:476561/1‑251 (MQ=255)                                                                                                                                                                             aCATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGGGGGGGTTGATATCGGTGGAACGGTACttt                                                  >  2:608357/1‑251 (MQ=255)                                                                                                                                                                             aCATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGAGGGGGTTGATATCGATGACACGGTACttt                                                  >  1:57568/1‑251 (MQ=255)                                                                                                                                                                                 aCTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACACATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTTGGGTTTGTTATCGATGACACGGGACttttttt                                              >  2:655536/1‑250 (MQ=255)                                                                                                                                                                                   tGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTcc                                             >  1:207671/1‑250 (MQ=255)                                                                                                                                                                                     aCTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGt                                          >  1:153746/1‑251 (MQ=255)                                                                                                                                                                                           tAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCGGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGGCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCggg                                     <  1:276049/250‑1 (MQ=255)                                                                                                                                                                                                               aTCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTTAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAAc                <  1:110802/251‑1 (MQ=255)                                                                                                                                                                                                               aTCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAAc                <  2:593772/251‑1 (MQ=255)                                                                                                                                                                                                                          aaTCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGcc       >  1:97555/1‑249 (MQ=255)                                                                                                                                                                                                                             cAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCCCCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGaaa  <  1:700263/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               TACATCGAGTAATAAACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCTCGCCAGAAA  >  NC_000913/4269217‑4269687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑