Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,265,668	C→T	T452I (ACC→ATC)	dnaB →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,265,668	0	C	T	98.8%	302.8 / NA	85	T452I (ACC→ATC)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (38/46);  minor base G (0/1);  total (38/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCACTGGCGAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTT  >  NC_000913/4265421‑4265907                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                gCACTGGCGAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCtt                                                                                                                                                                                                                                              <  1:442128/251‑1 (MQ=255)   aCTGGGGAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTTTATCGAGCAGGATGCGGACTTGATCATGTTTATCTAACGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTa                                                                                                                                                                                                                                            <  2:393958/251‑1 (MQ=255)         gAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTc                                                                                                                                                                                                                                      >  1:500173/1‑251 (MQ=255)         gAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTc                                                                                                                                                                                                                                      >  1:322019/1‑251 (MQ=255)            aGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAAt                                                                                                                                                                                                                                   >  1:523785/1‑251 (MQ=255)              aaCTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATgg                                                                                                                                                                                                                                 <  1:695620/251‑1 (MQ=255)                          cGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGAc                                                                                                                                                                                                                     <  1:4948/251‑1 (MQ=255)                                         cccAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGcc                                                                                                                                                                                                       <  1:607241/250‑1 (MQ=255)                                          ccAGTTGAACCGTTCTCTGGAACACCGTGCCGACAAACGCCCGGCAAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGc                                                                                                                                                                                                     >  2:701403/1‑251 (MQ=255)                                                gAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacg                                                                                                                                                                                               <  1:277284/251‑1 (MQ=255)                                                gAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTac                                                                                                                                                                                                <  1:592394/250‑1 (MQ=255)                                                gAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTGTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacg                                                                                                                                                                                               <  1:468807/251‑1 (MQ=255)                                                  aCCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgac                                                                                                                                                                                             <  2:536863/251‑1 (MQ=255)                                                     gtgctctGGAACAACGGGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgacgac                                                                                                                                                                                          <  2:267194/248‑1 (MQ=255)                                                     gTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgacgac                                                                                                                                                                                          <  1:427932/251‑1 (MQ=255)                                                       tctcTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgacgacg                                                                                                                                                                                         <  2:718895/250‑1 (MQ=255)                                                            ggAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCGTCGACAACTATGCGGGGCCGCAGTACGACGACGaat                                                                                                                                                                                      <  2:523785/248‑1 (MQ=255)                                                            ggAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGGGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGGCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaataat                                                                                                                                                                                   <  1:476478/251‑1 (MQ=255)                                                                caacGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGAGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAatt                                                                                                                                                                               >  1:257843/1‑251 (MQ=255)                                                                 aacGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAatta                                                                                                                                                                              <  2:322019/251‑1 (MQ=255)                                                                  acGTGCCGACAAAACCCCGGTCAACTCCGACCTGCGTGAATCGGGCTTGATCGAGCAGGATGCGGACTTGATCATGTTTTTCTATCTTGATGAGGTGTATCACGAAAACAGTGAGTTAAAAGGCATCGCGGAAATGATTATCGTTATACAACGTAACGGCCCATTCTGGACGGTACGCCTGATCTTTAACGGTCAATGTTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAattat                                                                                                                                                                             <  1:718345/251‑1 (MQ=255)                                                                   cGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAattatt                                                                                                                                                                            <  2:57263/251‑1 (MQ=255)                                                                   agTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAattatt                                                                                                                                                                            >  2:12179/2‑251 (MQ=255)                                                                      gCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTAtttt                                                                                                                                                                          >  1:333498/1‑250 (MQ=255)                                                                       ccGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTAt                                                                                                                                                                        <  1:278839/251‑1 (MQ=255)                                                                         gACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATg                                                                                                                                                                       >  1:549147/1‑250 (MQ=255)                                                                            aaaCGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAAtt                                                                                                                                                                   <  1:385733/251‑1 (MQ=255)                                                                             aaCGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTa                                                                                                                                                                  <  1:19360/251‑1 (MQ=255)                                                                                gCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGt                                                                                                                                                               <  2:140132/251‑1 (MQ=255)                                                                                 cccGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGt                                                                                                                                                               >  2:395604/1‑250 (MQ=255)                                                                                  ccGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTa                                                                                                                                                              >  2:231817/1‑250 (MQ=255)                                                                                    ggTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAAtt                                                                                                                                                           >  1:561579/1‑251 (MQ=255)                                                                                    ggTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAAtt                                                                                                                                                           >  1:20713/1‑251 (MQ=255)                                                                                         aCTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGCAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGACTAATAATTATTTTATGAATTAGGTAATTAAAGc                                                                                                                                                      >  2:417497/1‑251 (MQ=255)                                                                                          cTCCGACCTGCGTGCATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTCCGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGc                                                                                                                                                      >  2:449908/1‑250 (MQ=255)                                                                                            ccGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCaaa                                                                                                                                                   <  1:216955/251‑1 (MQ=255)                                                                                            ccGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCa                                                                                                                                                     <  2:536977/249‑1 (MQ=255)                                                                                             cGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAac                                                                                                                                                  <  1:126959/251‑1 (MQ=255)                                                                                             cGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCAGGTTTAGCTATCGTGATGAGGTTTAGCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGAGCTGTAACGGTCAATGGTCGCGCTTCGTCAACGATGCGGGGCCGGAGTACGACGACGAATAAGAATGATTTTATGAATTAGGTAATTAAAGCAAac                                                                                                                                                  <  2:50542/251‑1 (MQ=255)                                                                                              gACCTGCTTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGTAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAaca                                                                                                                                                 <  2:52793/251‑1 (MQ=255)                                                                                              gACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAac                                                                                                                                                  >  2:161313/1‑250 (MQ=255)                                                                                              gACCGGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAaca                                                                                                                                                 <  2:680465/251‑1 (MQ=255)                                                                                                       gAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAg                                                                                                                                        <  1:618280/251‑1 (MQ=255)                                                                                                         aTCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAgg                                                                                                                                       >  2:630043/1‑250 (MQ=255)                                                                                                             ggCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAacac                                                                                                                                  >  1:358406/1‑251 (MQ=255)                                                                                                             ggCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGGATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTATCGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGTCGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACTCTTATCAAGGAaca                                                                                                                                   <  1:475506/250‑1 (MQ=255)                                                                                                                         cAGGATGCGGCCTTGATCATGTTTATCTATCGTGCTGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCgg                                                                                                                      >  1:226896/1‑251 (MQ=255)                                                                                                                               gCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGTAACACAAATGCAAGCGGCAACt                                                                                                                 <  2:288594/250‑1 (MQ=255)                                                                                                                                  gACTTGAACATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGAGTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGTTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACCTATCAAGGAACACAAATGCAAGCGGCAACtgttg                                                                                                             <  2:278456/251‑1 (MQ=255)                                                                                                                                   aCTTGATCATGTTTATCTATCGTGATGAGGTGTAACACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACtgttgt                                                                                                            <  2:328354/251‑1 (MQ=255)                                                                                                                                       gATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGAt                                                                                                         <  1:301818/250‑1 (MQ=255)                                                                                                                                           aTGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAAcc                                                                                                    <  1:538303/251‑1 (MQ=255)                                                                                                                                           aTGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGGAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAAcc                                                                                                    >  2:598462/1‑251 (MQ=255)                                                                                                                                                           atgaGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGac                                                                                     >  1:235099/1‑250 (MQ=255)                                                                                                                                                              aggggtATCACGAAAACATTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACCCTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACaa                                                                                 <  1:417497/247‑1 (MQ=255)                                                                                                                                                              aGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGAcaca                                                                                  >  2:202969/1‑250 (MQ=255)                                                                                                                                                              aGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGAcaca                                                                                  >  2:621224/1‑250 (MQ=255)                                                                                                                                                                 tgtATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCt                                                                              >  1:575451/1‑251 (MQ=255)                                                                                                                                                                  gtATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTg                                                                             >  1:211354/1‑251 (MQ=255)                                                                                                                                                                   tATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTCACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCCACTGTTGTGATTAACCGCCGCGCTCTGCGCCACAACCTGc                                                                            >  1:182698/1‑251 (MQ=255)                                                                                                                                                                       aCGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGAAAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAATCTGCAACg                                                                        <  2:476446/251‑1 (MQ=255)                                                                                                                                                                        cGAACACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCCGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACCCTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGCCACAACCTGCAACGt                                                                       >  2:358350/1‑251 (MQ=255)                                                                                                                                                                          aaaaCAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCt                                                                     <  1:530434/251‑1 (MQ=255)                                                                                                                                                                            aaCAGTGATTTAAAAGGCATCGCGGAAATTCTTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCACCGTCTTc                                                                   >  2:71554/1‑251 (MQ=255)                                                                                                                                                                            aaCAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTc                                                                   <  2:594610/251‑1 (MQ=255)                                                                                                                                                                              cAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGt                                                                 >  2:182124/1‑251 (MQ=255)                                                                                                                                                                                        aaaGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAAGGGTCGCGCTTCGACAACAATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGccc                                                       <  2:215775/251‑1 (MQ=255)                                                                                                                                                                                             cATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGcc                                                  >  2:162513/1‑251 (MQ=255)                                                                                                                                                                                              aTCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCa                                                 <  1:427286/251‑1 (MQ=255)                                                                                                                                                                                                 gcgGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTa                                              <  1:231817/251‑1 (MQ=255)                                                                                                                                                                                                  cgGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTa                                              >  2:277347/1‑250 (MQ=255)                                                                                                                                                                                                         tattatCGGTAAACAACGAAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGGCTTCGTGAACTGGCCCCTGCCAGTAAAATGGt                                       <  1:630043/250‑1 (MQ=255)                                                                                                                                                                                                          attatCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTg                                     >  2:140059/1‑251 (MQ=255)                                                                                                                                                                                                                  tAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGgtggtg                             >  1:345900/1‑251 (MQ=255)                                                                                                                                                                                                                  tAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGgtggtg                             >  1:434463/1‑251 (MQ=255)                                                                                                                                                                                                                      caacGAAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGTTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACTCTTATCAAGGAACACAAATGCAAGCGGCAACTTTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACTGCGTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTTGTGAAAg                         <  1:646150/251‑1 (MQ=255)                                                                                                                                                                                                                       aacGTAACGGCACAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAACAAACACTTATCAAGGAAAAAAAATGCAAGCGGACACTGTTGTGATTAACCGCCGCGCTCTGCGACAAAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGGAAAATGGTTGCGGTGGGGAAAGc                        >  1:240892/1‑251 (MQ=255)                                                                                                                                                                                                                          gTAACGGCCCAATCGGGAGGTTGCGCCTGATCTTTAACGGGCAATGGTCGCGCTTCGACAACTAGGCGGGGCCGCAGTACGACGACGAAAAATAATTATTTTATGAATTAGGTAATAAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGGTGTGATTAACCGCCGCGCTCTGCGAAACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGaa                     <  1:424067/251‑1 (MQ=255)                                                                                                                                                                                                                          gTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGa                      >  2:103204/1‑250 (MQ=255)                                                                                                                                                                                                                                   cAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATgg            <  1:103204/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCa         <  1:395604/250‑1 (MQ=255)                                                                                                                                                                                                                                         ggACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACCACCTGCCACGTCTTCGTGAACTGGCCCCTGCCAGTCAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACgg      >  1:121288/1‑251 (MQ=255)                                                                                                                                                                                                                                           aCGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGtc    >  1:177668/1‑251 (MQ=255)                                                                                                                                                                                                                                            cGGTACGCCGGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGtct   <  1:701403/251‑1 (MQ=255)                                                                                                                                                                                                                                             ggTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGATGCGGTGGTGAAAGCGAACGCTTATGGTCACGGtctt  >  2:397891/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                GCACTGGCGAAAGAACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCCGACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCGCTCTGCGACACAACCTGCAACGTCTTCGTGAACTGGCCCCTGCCAGTAAAATGGTTGCGGTGGTGAAAGCGAACGCTTATGGTCACGGTCTT  >  NC_000913/4265421‑4265907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑