Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,249,684	C→T	A44V (GCT→GTT)	malM →	maltose regulon periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,249,684	0	C	T	100.0%	278.5 / NA	79	A44V (GCT→GTT)	malM	maltose regulon periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/40);  total (39/40)

CATCCGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGCTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTC  >  NC_000913/4249438‑4249925                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   cATCCGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTgttgcg                                                                                                                                                                                                                                                >  2:443311/1‑251 (MQ=255) cATCCGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTgttgcg                                                                                                                                                                                                                                                >  1:410525/1‑251 (MQ=255)           aaaaaCAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCaacaac                                                                                                                                                                                                                                      >  2:734242/1‑251 (MQ=255)            aaaaCAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACt                                                                                                                                                                                                                                     <  2:319335/251‑1 (MQ=255)              aaCAGGTTGTCATTGTCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTAGCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTCCCGCAAACCAACAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTc                                                                                                                                                                                                                                    >  2:86734/1‑250 (MQ=255)                cAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCAcc                                                                                                                                                                                                                                 <  1:724909/251‑1 (MQ=255)                 aGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCt                                                                                                                                                                                                                                <  1:392577/251‑1 (MQ=255)                   gTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTgg                                                                                                                                                                                                                              <  2:84511/251‑1 (MQ=255)                      gTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGaca                                                                                                                                                                                                                           <  1:260260/251‑1 (MQ=255)                       tCATTATCTGAAACCGGCGAAGGCCCCTATGATTATCGGGTCTAGCGCGCTATTGCCTGGCTACCACTGAGCTCCAGATTTTGAGGCGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCATTGCCGATGTTAACTACGTACCGCAAAACACCAGAGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTgaaacc                                                                                                                                                                                                                          >  2:707750/1‑246 (MQ=255)                           tATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCgg                                                                                                                                                                                                                       <  1:339004/250‑1 (MQ=255)                                     gggCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGGCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGGACCGCAAAACACCAGCGACGCTCCAGCCATTCCATGTGTTGCGCTGCAACAACTCGCCTTGTTTCCGGTCGATCAATCt                                                                                                                                                                                                            <  2:41023/251‑1 (MQ=255)                                      ggCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGAGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGTCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGCCGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGCTCCATCt                                                                                                                                                                                                            >  1:362359/1‑250 (MQ=255)                                              ccccTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAACCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTACATCTGTTGCGCTGCAACAACTCACCTGGCCACCGGTCGATCAATCTAAAACCCa                                                                                                                                                                                                    >  1:504888/1‑250 (MQ=255)                                               cccTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAAccc                                                                                                                                                                                                     >  1:426506/1‑248 (MQ=255)                                                        tATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAAc                                                                                                                                                                                         >  1:581541/1‑251 (MQ=255)                                                                 tAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACGGGCGAcc                                                                                                                                                                                 <  1:212757/250‑1 (MQ=255)                                                                  aGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACcgg                                                                                                                                                                               <  2:559787/251‑1 (MQ=255)                                                                        cTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGcc                                                                                                                                                                          >  1:170664/1‑250 (MQ=255)                                                                            tGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCcaaca                                                                                                                                                                      >  2:635850/1‑250 (MQ=255)                                                                                 ggCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGa                                                                                                                                                                <  2:581541/250‑1 (MQ=255)                                                                                  gCTACCGCTGAGCTCCAGATTTTGAGGTGACAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAAc                                                                                                                                                              >  1:553990/1‑251 (MQ=255)                                                                                                  aGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTg                                                                                                                                              <  1:697866/251‑1 (MQ=255)                                                                                                   gATTTTGAGGTGAAAACAATGAAAATGAATAACAGTCTCATCGTCCTCTGTTTATCAGCATGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTgg                                                                                                                                             <  1:197019/251‑1 (MQ=255)                                                                                                     ttttGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAACACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAACCCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTc                                                                                                                                           >  2:175630/1‑251 (MQ=255)                                                                                                          aGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGtt                                                                                                                                      <  1:82210/251‑1 (MQ=255)                                                                                                               aaaaCAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTtgctgc                                                                                                                                 >  2:179115/1‑251 (MQ=255)                                                                                                               aaaaCAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTtgctgc                                                                                                                                 <  1:443311/251‑1 (MQ=255)                                                                                                                  aCAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTa                                                                                                                              <  1:710174/251‑1 (MQ=255)                                                                                                                    aatgaacatgacTAAACGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTAcc                                                                                                                            >  2:82982/1‑250 (MQ=255)                                                                                                                    aatgaaaatgaaTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACa                                                                                                                            <  1:623078/251‑1 (MQ=255)                                                                                                                    aatgaaaatgaaTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACa                                                                                                                            <  1:642653/251‑1 (MQ=255)                                                                                                                      tgaaaatgaaTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGc                                                                                                                          >  1:212064/1‑251 (MQ=255)                                                                                                                       gaaaatgaaTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCCGCGTACAGCg                                                                                                                         >  2:685333/1‑251 (MQ=255)                                                                                                                         aaatgaaTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTc                                                                                                                       >  1:653036/1‑251 (MQ=255)                                                                                                                          aatgaaTAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAC‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTcc                                                                                                                      >  1:163346/1‑251 (MQ=255)                                                                                                                                    aGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACAt                                                                                                            >  1:158266/1‑251 (MQ=255)                                                                                                                                     gTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACAtt                                                                                                           <  1:129948/251‑1 (MQ=255)                                                                                                                                          aTCGTACTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGCATTAACCTTGCCGATGCTACCTCCGTACCGCCAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGACGATAAATCTAAAACCCAGACCACCCACCTGGCGACCGG‑AGCCACCAAACTGAACGTTCACGGCATCAAAGGTCCGGTTGCTGCGTACAGCGTCCCGGCAACCATTGGCg                                                                                                       >  2:624269/1‑250 (MQ=255)                                                                                                                                           tCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGtt                                                                                                                                      >  2:449723/1‑218 (MQ=255)                                                                                                                                           tCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGtt                                                                                                                                      <  1:449723/218‑1 (MQ=255)                                                                                                                                                  ctGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGAccc                                                                                              >  1:30917/1‑251 (MQ=255)                                                                                                                                                    gTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGAcc                                                                                               <  2:605357/248‑1 (MQ=255)                                                                                                                                                    gTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGGCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGAccc                                                                                              <  2:163346/249‑1 (MQ=255)                                                                                                                                                             cagGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGAcc                                                                                   >  1:267397/1‑251 (MQ=255)                                                                                                                                                              agGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGGACCGCAAAACACCAGCGACGCGCCTGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACGCCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAAAAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCa                                                                                  <  2:721524/251‑1 (MQ=255)                                                                                                                                                                ggTTACTGGCAAACGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACCCCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGGCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGATGACCAGc                                                                                <  2:217464/251‑1 (MQ=255)                                                                                                                                                                  ttACTAGCCAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGACAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGAAACCGGTCGATCAATCTAAAACCAAGACCACCCAAATGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAACCATTGGCGAACTGACCCTGACGCAGACAAGCg                                                                               >  2:60067/1‑250 (MQ=255)                                                                                                                                                                   tACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCCCCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGa                                                                              <  2:170664/250‑1 (MQ=255)                                                                                                                                                                      tGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGAACCTGCCGCTGACCAGCGAAgcg                                                                          >  2:473760/1‑249 (MQ=255)                                                                                                                                                                       ggCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACGCCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGt                                                                           <  2:557455/249‑1 (MQ=255)                                                                                                                                                                       ggCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAAATACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGt                                                                           <  2:506816/249‑1 (MQ=255)                                                                                                                                                                        gCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGa                                                                         >  1:521433/1‑250 (MQ=255)                                                                                                                                                                            gcgcgcCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACGCTGACGCTGACCAGCGAAGTGaacaaa                                                                    <  2:479300/251‑1 (MQ=255)                                                                                                                                                                                gcCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAAcaaacaaa                                                                <  1:200573/251‑1 (MQ=255)                                                                                                                                                                                    ggAATTAGCCTTGCCGATGTTAACTACGTACAGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAAcc                                                              >  1:177032/1‑249 (MQ=255)                                                                                                                                                                                     gAATTAGCCTTGCCGCTGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAACACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGCGAACAAACAACCCAGc                                                           >  2:60790/1‑251 (MQ=255)                                                                                                                                                                                     gAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGc                                                           <  2:303345/251‑1 (MQ=255)                                                                                                                                                                                       aTTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGt                                                         >  2:629030/1‑251 (MQ=255)                                                                                                                                                                                          aGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGttt                                                       <  1:179115/250‑1 (MQ=255)                                                                                                                                                                                             cTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTgc                                                   >  2:702500/1‑251 (MQ=255)                                                                                                                                                                                               tGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGGCCACCCAACTGTCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGAACATCGATTTGTATAAACAATCCAGCGTTTTGgcg                                                  <  2:491279/250‑1 (MQ=255)                                                                                                                                                                                                    aTGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGtt                                                        <  1:579083/239‑1 (MQ=255)                                                                                                                                                                                                    aTGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTCGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGtt                                                        >  2:579083/1‑239 (MQ=255)                                                                                                                                                                                                        tAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGc                                        >  2:37883/1‑251 (MQ=255)                                                                                                                                                                                                             aCGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGAtt                                   <  1:224689/251‑1 (MQ=255)                                                                                                                                                                                                               gTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCt                                 <  1:82982/251‑1 (MQ=255)                                                                                                                                                                                                                tACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCtt                                >  1:391371/1‑251 (MQ=255)                                                                                                                                                                                                                 aCCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGTCCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTg                               <  2:410525/251‑1 (MQ=255)                                                                                                                                                                                                                    gaaaaaCACCAGCGACGCGCCAGCCATTCCATCTGTCGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTGGTTGCTCCGAACGTGCTGATTCTTGATc                            <  2:651574/249‑1 (MQ=255)                                                                                                                                                                                                                         acacCAGCGACGCGCCCGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACCCCGGTCGCTCACTCTAACACCCACACCACCCAACTGGCGACAGGCGGCCAACAA‑CTGACCGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCACGGCAAACATTGGCGACCTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGAGCCGAACGTGCTATTTCTTGATCAGAAc                       >  2:739531/1‑251 (MQ=255)                                                                                                                                                                                                                                cGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCCCCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGAccc                <  2:232895/251‑1 (MQ=255)                                                                                                                                                                                                                                cGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATGGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGAccc                <  2:267397/251‑1 (MQ=255)                                                                                                                                                                                                                                 gACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGAcccc               >  2:644585/1‑251 (MQ=255)                                                                                                                                                                                                                                   cgcgCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCAt             >  2:82466/1‑251 (MQ=255)                                                                                                                                                                                                                                     cgcCAGCCATTCCATCTGTTGCGCTGCAACAACCCACCTGGACACCGGGCGATCAATCTAAAACCCAGACCACCCACCTGGCGACCGGCGGCCAACAA‑CTGAACGCTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGGCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAATCCAGCGTTTTTGCTCCGAACGTGCTGATTCTTGATCAggatttgagcccatca           <  2:118410/251‑16 (MQ=255)                                                                                                                                                                                                                                        cagccaTTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGACCGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGcc        >  1:58993/1‑251 (MQ=255)                                                                                                                                                                                                                                         agccaTTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCct       <  2:230451/251‑1 (MQ=255)                                                                                                                                                                                                                                             aTTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCcttctt   >  1:416440/1‑251 (MQ=255)                                                                                                                                                                                                                                              ttCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCttcttc  >  1:602797/1‑251 (MQ=255)                                                                                                                                                                                                                                              ttCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCttcttc  >  1:79261/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   CATCCGGCATAAAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGCTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAA‑CTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTC  >  NC_000913/4249438‑4249925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑