| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,219,752 | C→T | S386F (TCC→TTC) | aceK → | isocitrate dehydrogenase kinase/phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,219,752 | 0 | C | T | 98.4% | 209.9 / NA | 63 | S386F (TCC→TTC) | aceK | isocitrate dehydrogenase kinase/phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (29/33); minor base G (0/1); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCA > NC_000913/4219520‑4219998 | aattaGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAAtt < 1:408847/247‑1 (MQ=255) agtttcatGAAGCGCCGGGTATTCTTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATATGGTATTCAAAGTCATCAAAGACAGGTGCGCGACGCAGAAGGAGATGTCTGCCGATCACGTTCGTGCCTGCTATCAACTGTTGAAAGAGCACGAGCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCtt < 2:576604/244‑1 (MQ=255) tGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGaa < 2:228840/250‑1 (MQ=255) aaGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGAGCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAACCTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAagca < 1:687622/251‑1 (MQ=255) cgcCGGGTATTCGTGGATTGGTGATGGTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCGCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGTTCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGTAATTACTGCTGCAGGAAGCAGCg < 2:437965/251‑1 (MQ=255) tATTCGTGGAATGGTGATGTTGGTGTTTACGCCGCCGGGCTTTGATCGGGTATTCAAAGTCATAAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGGGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTAATGCTTCAGGAAGCAGCGGaaaaa > 2:182973/1‑250 (MQ=255) aTGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGAAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGAtct > 1:273351/1‑251 (MQ=255) ggTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCg > 1:131307/1‑251 (MQ=255) ggTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCg > 2:498988/1‑251 (MQ=255) tGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGc > 1:552217/1‑251 (MQ=255) ggATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCATCGGAAAAAATCACCGATCTCGGc < 2:493223/250‑1 (MQ=255) gATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGc < 1:498988/250‑1 (MQ=255) ggTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACaa > 2:639572/1‑250 (MQ=255) gCTGCCGGGCTTTGCTCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTc > 2:679971/1‑251 (MQ=255) cGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGCTCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCAt > 2:723565/1‑251 (MQ=255) tttGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCttt > 1:204230/1‑250 (MQ=255) ttGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCttt < 2:177530/249‑1 (MQ=255) tGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTtat > 1:574256/1‑250 (MQ=255) tGATCGGGTATTCAAAGTCATAAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTtat > 2:329650/1‑250 (MQ=255) aTCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATAtt > 1:390750/1‑251 (MQ=255) aaGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCCGCTATCAACTGGTGAAAGCGCACGATCGCGTGGGCCGAATGGCAGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACAGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGCTGGAGACGCTc > 1:318728/1‑251 (MQ=255) cgccgcAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATctggctg > 1:585680/1‑251 (MQ=255) ccgcAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGa < 1:558117/251‑1 (MQ=255) cAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGCAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAATTCAACGATCTCGGCGAACAAATTGTGATTCGACATCTTTATATTGAGCGGCGGATGGTACCGCTCACTATctggctggcac > 2:287252/1‑247 (MQ=255) cAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACa < 1:201137/251‑1 (MQ=255) gagaTGTCTGCCGATCCCGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGAAACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGCGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTgg > 2:700226/1‑250 (MQ=255) aTGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAgg > 2:582067/1‑251 (MQ=255) ggcTGCCGCCCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTc < 2:194875/249‑1 (MQ=255) cACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTgc < 1:8115/250‑1 (MQ=255) gTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGcc < 1:23899/251‑1 (MQ=255) ccTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCAtt < 1:679971/251‑1 (MQ=255) cTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAACTCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTg > 2:566339/1‑251 (MQ=255) agcAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTGCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGTCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATTGTGCCGCTCAATATCAGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTgaagaa < 2:318250/249‑1 (MQ=255) aaCTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATAc > 2:244777/1‑251 (MQ=255) ggTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTc > 2:579723/1‑155 (MQ=255) ggTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTc < 1:579723/155‑1 (MQ=255) tGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAAc > 2:69320/1‑251 (MQ=255) gagcacgctcgagtgaggagAATGGCCGAAACCCCGGAGTTTGAAAACTTTGTGCAGGCGAAGCGGCATCTTTTCCCGGCATTAATGGACTTACTGCTTAAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACACATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCAGCTCAATATCTGGCTGGAACACGTGGACGGTAAGCAGTTGCGCGGCGCCATTGAAGAATACGGTAACGCTAt > 2:115722/20‑251 (MQ=255) agCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTAtt < 1:723565/251‑1 (MQ=255) gCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTc < 1:69320/251‑1 (MQ=255) cACGCTCGCGTGGGCCGAATGGCAGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGCAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCCGACAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGTTCAGAAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCg < 2:168431/251‑1 (MQ=255) cACGATCGCGTGGGCCGAATGGCGGACACCCATGATTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATCGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGTTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCg < 1:624974/251‑1 (MQ=255) cgcgTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAAGCACCGATCGCGGCGAACAAATTGTTATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGGGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCt < 2:732952/251‑1 (MQ=255) gTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGc < 2:363993/251‑1 (MQ=255) gggCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTAGTCGCCAGCTTGcc < 2:131307/250‑1 (MQ=255) cGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCgctgc > 2:567526/1‑251 (MQ=255) gAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCAGTAATTGAATTACTGCTTCAGGATGCAGCGGAAAAAGTCACCGATCTCGGCGAACAATTTGTGATTCTCGATCTTTGTATTGAGCGGCGGATGTTTCCGCTCAATATCTGGCTGGAACAGGGGGAAGGTCAGCAGTTGCGCAACACCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCgctgct < 1:430282/251‑1 (MQ=255) cGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACACGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACAtt > 1:665514/1‑251 (MQ=255) cGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACAtt < 1:329650/251‑1 (MQ=255) aGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGAc > 2:315177/1‑251 (MQ=255) tGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATg < 1:536098/250‑1 (MQ=255) aCTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGttt < 1:182973/251‑1 (MQ=255) aCTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGttt > 2:271526/1‑251 (MQ=255) gtgtgCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTa < 1:642574/248‑1 (MQ=255) tgtgCTGGAGAAGCGGCATACTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATGCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGTTCAGCAGTTGCGCGATGCCATTGAAGAATACGGTAACGCGATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCGGTTTaaa < 2:286521/250‑1 (MQ=255) gCTGGAGAAGCGGCATATTTTCCCGGCATTAATGTAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGCCGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAAc < 1:274325/250‑1 (MQ=255) gagaAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCgg < 2:60180/251‑1 (MQ=255) gaAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGAGAACAACTTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGTCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGACTACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGCCATGCTGTTTAAAAACTTCGgtg > 2:38780/1‑251 (MQ=255) aGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTc < 2:585680/251‑1 (MQ=255) cGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCAACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCAc < 2:215898/251‑1 (MQ=255) cGGCAGATTTTCCCGGCACTAATGGAATTACTGCTTCATGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGGTGGTGGCGCGCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCGTTGAAGAATACTGTAACGGTATTCGCCAGCTTTCCTCTGCTAACATTTTCCCTGTCGACATGCTGTTTAAAAACTTCGGTGTCAc < 1:698942/251‑1 (MQ=255) cATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCg > 2:39213/1‑251 (MQ=255) atTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCCGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTCAAAACTTCGGTGTCACCCGTcc > 1:92263/1‑250 (MQ=255) | AATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCGCGACGCCATTGAAGAATACGGTAACGCTATTCGCCAGCTTGCCGCTGCTAACATTTTCCCTGGCGACATGCTGTTTAAAAACTTCGGTGTCACCCGTCA > NC_000913/4219520‑4219998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |