Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,941,874	C→T	noncoding (67/1542 nt)	rrsC →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,941,874	0	C	T	98.0%	124.4 / NA	50	noncoding (67/1542 nt)	rrsC	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (26/23);  minor base A (1/0);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGC  >  NC_000913/3941628‑3942118                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      cacaCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGt                                                                                                                                                                                                                                                    >  2:392625/1‑250 (MQ=14)    cccccGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACATTTTATCAGACAATCTGTGTGTGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACAGGCAAGTCGAATGGTAACa                                                                                                                                                                                                                                                <  1:501280/250‑1 (MQ=9)        cgcgCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑Gaa                                                                                                                                                                                                                                           <  1:322982/251‑1 (MQ=14)           gccgcTGAGAAAAAGCAAAGCGGCAGTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAAGGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTGTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACa                                                                                                                                                                                                                                        <  2:525559/251‑1 (MQ=12)                 gagaAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTg                                                                                                                                                                                                                                   <  2:407478/250‑1 (MQ=18)                   gaAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGGCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATGCAATTCGAATGGTAACAG‑GAAACAGCTTGCTg                                                                                                                                                                                                                                <  2:261824/251‑1 (MQ=16)                        agcaaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCg                                                                                                                                                                                                                           >  1:588908/1‑251 (MQ=21)                        agcaaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCGCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCAAGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCg                                                                                                                                                                                                                           <  1:440005/251‑1 (MQ=21)                           aaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGACAAATGAATACCAAGTCTCCAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGCACGCTGGCGGCAGGCCTAACACATGCACGTCGAATGGTAACCG‑GACACAACTTGCTGTTTCGCTg                                                                                                                                                                                                                        >  1:438603/1‑251 (MQ=11)                           aaagcGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTg                                                                                                                                                                                                                        >  1:508402/1‑251 (MQ=18)                                                                                                                                            tAATTCATTACGAAGTTTAATTCCTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGGGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTc                                                                                                       >  1:539330/1‑251 (MQ=14)                                                                                                                                                    tACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACAAAAGAGGGGGACCTTCGGGCctct                                                                                               >  2:273181/1‑251 (MQ=14)                                                                                                                                                     aCGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTGTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAAGGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGTCctct                                                                                               <  2:302189/250‑1 (MQ=9)                                                                                                                                                         aGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGcc                                                                                           >  1:318183/1‑250 (MQ=14)                                                                                                                                                            ttAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATc                                                                                        <  1:351344/250‑1 (MQ=14)                                                                                                                                                              aaTTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGGCGCAAGACCAAAGAGGTGGACCTTCGGGCCTCTTGCCATCAg                                                                                      <  2:508402/250‑1 (MQ=17)                                                                                                                                                               aTTCTTTGAGCATCAAACTTTTAAAGTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGGCGAATGGTAACAG‑GAAACAGCGTGCTGGTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTTGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCGTCGGGCCTCTTGCCAGCAGAt                                                                                    <  2:438603/251‑1 (MQ=11)                                                                                                                                                                  cTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGAAGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAAAGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGAtggg                                                                                 >  2:614080/1‑249 (MQ=16)                                                                                                                                                                       aGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCAGCAGATGTGCCCAg                                                                            <  2:55598/251‑1 (MQ=18)                                                                                                                                                                       aGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGGGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATAACGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGccc                                                                              >  2:356324/1‑249 (MQ=18)                                                                                                                                                                         cATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGccc                                                                              >  1:675356/1‑247 (MQ=18)                                                                                                                                                                          aTCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCCGATGTGCCCAGATg                                                                         >  2:537754/1‑251 (MQ=14)                                                                                                                                                                            cAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGACATCAGATGTGCCCAGATggg                                                                       >  2:137561/1‑251 (MQ=18)                                                                                                                                                                                             gagTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACCTGCACGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGCCGAGTGGCGGACGGGTGAGTAATGTCTGGGAACCTGCCTGATGGCGGGGGATAACTACTGGAAACGGTAGCTAATACCGCGTAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTggg                                                      >  1:304917/1‑251 (MQ=11)                                                                                                                                                                                                tttGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTc                                                                                                       <  1:448918/199‑1 (MQ=14)                                                                                                                                                                                                tttGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGGGCGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATCACGTCGCAAGACCAAAGAGGGGGACCTTc                                                                                                       >  2:448918/1‑199 (MQ=9)                                                                                                                                                                                                  tGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAGCACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTaa                                                  <  2:174827/250‑1 (MQ=37)                                                                                                                                                                                                   gATCATGGCTCAGATTGACCGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGCTAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGATAGTAGGTGGGGTAACg                                                >  1:593516/1‑251 (MQ=255)                                                                                                                                                                                                   gATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGGCGAATGGTAACAG‑TAAACAGCTTGCTGGTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTTATGGAGGGGGATAACTACTGGAAACGGTAGCGAATACCGCATAACGTCGCAGGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAAc                                                 <  1:454332/250‑1 (MQ=11)                                                                                                                                                                                                        tGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTc                                            >  1:294728/1‑250 (MQ=37)                                                                                                                                                                                                          gCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAcc                                         <  1:108514/251‑1 (MQ=37)                                                                                                                                                                                                             cAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCt                                        <  1:6905/249‑1 (MQ=38)                                                                                                                                                                                                                   gAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcgac                                 <  1:374167/250‑1 (MQ=39)                                                                                                                                                                                                                   gAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcgac                                 <  1:553539/250‑1 (MQ=39)                                                                                                                                                                                                                       gCTGGCGGCAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATcc                            >  1:368844/1‑251 (MQ=38)                                                                                                                                                                                                                       gCTGGCGGCACGCCTATCACATGCAACTCGAAAGGTACCAG‑AAAACAGCTTGATGTTTCGATGACGCGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGCTGGAGGGGGATCACTACTGGCAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTTGTCGGTGGGGTAACGGCTCACCTAGGCGACGATcc                            >  2:702927/1‑251 (MQ=11)                                                                                                                                                                                                                            cggcAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGAGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCCTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTCGGCGACGATCCCTAGc                       >  1:19730/1‑251 (MQ=255)                                                                                                                                                                                                                              gcAGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTg                     <  1:547116/251‑1 (MQ=38)                                                                                                                                                                                                                                aGGCCTAACACATGCACGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGt                   >  1:620303/1‑251 (MQ=255)                                                                                                                                                                                                                                aGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTg                                                        <  2:37879/214‑1 (MQ=38)                                                                                                                                                                                                                                aGGCCTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTg                                                        >  1:37879/1‑214 (MQ=38)                                                                                                                                                                                                                                    cTAACACATGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGAGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACAAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTAACCTAGGCGACGATCCCTAGCTGGTCTg                >  1:295129/1‑250 (MQ=255)                                                                                                                                                                                                                                        cacaTGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAgg           >  1:484068/1‑251 (MQ=38)                                                                                                                                                                                                                                        cacaTGCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGAGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGGGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGCGAgg           >  2:529850/1‑251 (MQ=255)                                                                                                                                                                                                                                            aacAAGTCGAATGATAACAGCGAAA‑AGCTTGCGGTAACGCTGACGAGTGGCGAACGGGGGAGTAATGTATGGGAAAATGGCTGATGGAGGGGGATACATACTGGAAACGGTAGCTAATACCGCATAACGGCGCAAGACAAAAGAGTGGGACCTTCGGGCCTCTTGCCAACAGAAGTGACCCGAGTGGATTATCTAGTAGGTGGAGTAACTGCTCACCTAGGCGAAGATCCCTAGGTGGACTGAGAGGATg        >  2:151694/3‑250 (MQ=2)                                                                                                                                                                                                                                             gCAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGAc      <  1:361634/251‑1 (MQ=38)                                                                                                                                                                                                                                              cAAGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGACTGGGGAACTGCCTGGTGGAGGGGGCTAACTACTGTAAACGGTAGCTAATACCGCATAACGTCGCCAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATAAGATGTGCCCAGATGGGATTAGCTCGTAGGGGGGGGAACGGCTAACCTAGGCGACGATCCCTAGCTTGTCTGCGAGGATGaac     >  2:284320/1‑249 (MQ=11)                                                                                                                                                                                                                                                aGTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATGAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGAGCCCTAGCTGGTCTGATAGGATGACCAg   <  2:51007/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gTCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGTGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGGTTTGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGGTCCCTAGCTGGTCTGAGAGGGTGACCAGc  >  2:581159/1‑251 (MQ=255)                                                                                                                                                                                                                                                  tCGAATGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGAt                              <  2:89372/222‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAG‑GAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGC  >  NC_000913/3941628‑3942118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑