Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,937,997	G→A	G235D (GGT→GAT)	rbsK →	ribokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,937,997	0	G	A	99.0%	326.1 / NA	95	G235D (GGT→GAT)	rbsK	ribokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (40/54);  minor base C (1/0);  total (41/54)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

AAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAGCGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGGTGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGA  >  NC_000913/3937753‑3938243                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       aaaaTCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACAGTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAgg                                                                                                                                                                                                                                                  <  1:271892/251‑1 (MQ=255)      cGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGGATTCGTGTTGAAAATGATGAAGATACAACGAAGGCGGCGCAGGGACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAgc                                                                                                                                                                                                                                             >  2:259662/1‑251 (MQ=255)         ccATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCAAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTAATGCCTGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGGCGTGGTGTATGGGCGAGCGTGAATGATGAAGGTAAGcgcg                                                                                                                                                                                                                                          >  2:496882/1‑251 (MQ=255)             cAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTcc                                                                                                                                                                                                                                      <  2:389301/251‑1 (MQ=255)               aaaTAAGACTATCGTTGCGCTTCACCCGGCTCCGGCTCGCGAACTTCCTGACTAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATTAAGGTCAGCGCGTTCCTg                                                                                                                                                                                                                                    <  1:642815/251‑1 (MQ=255)                 aTAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGATGGCGATGGAGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTCTTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGCACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGCCGTGGTGTATGGGCTAGCGTGAATGAGGAAGGTCAGCGCGTTCCTgg                                                                                                                                                                                                                                   >  2:25932/1‑250 (MQ=255)                  tAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGAt                                                                                                                                                                                                                                 >  2:38314/1‑251 (MQ=255)                      aCTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACCTTATTACGCCAAACGAAACGGCAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCg                                                                                                                                                                                                                             >  1:353235/1‑251 (MQ=255)                       cTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGCTTCCgg                                                                                                                                                                                                                            >  1:121897/1‑251 (MQ=255)                         aTCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCgggg                                                                                                                                                                                                                          >  1:674766/1‑250 (MQ=255)                              tGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCTCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCATCGAATGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCTGGTGCAg                                                                                                                                                                                                                      <  1:680967/250‑1 (MQ=255)                                    tAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTc                                                                                                                                                                                                                <  2:213189/250‑1 (MQ=255)                                     aaCCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAGGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCg                                                                                                                                                                                                               <  1:453563/250‑1 (MQ=255)                                        ccggctccggctCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATAc                                                                                                                                                                                                           >  1:298621/1‑251 (MQ=255)                                        ccggctccggctCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATAc                                                                                                                                                                                                           >  1:6402/1‑251 (MQ=255)                                          ggctccggctcGCGAACGTCCTGACGAACTGCTGGCGCTGGTGGACAATATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTTCTGTAGTGATTGCTTTAGGAAGTCGTGGTGTATGGGCTAGCTTGAATGATGAAGGTCTGCGCGGACCTGGATTGCGGGTGCAGGCTGTTGATTCCa                                                                                                                                                                                                         <  2:67057/251‑1 (MQ=255)                                                   tcGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCg                                                                                                                                                                                                >  1:642798/1‑251 (MQ=255)                                                    cgcgAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCgg                                                                                                                                                                                               >  1:438373/1‑251 (MQ=255)                                                      cgAACTTCCTGACGAACTGCTGGCGCTGGAGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGGGAATGATGAAGGTCAGCGCGTTCCTGGAGTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGgag                                                                                                                                                                                             <  2:353235/251‑1 (MQ=255)                                                       gAACTTCCTGACGCACTACTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGAGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCgg                                                                                                                                                                                               >  2:585427/1‑248 (MQ=255)                                                         aCTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATa                                                                                                                                                                                          <  1:387444/251‑1 (MQ=255)                                                         aCTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGAATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGTCTGTCGATACCATTGCTGCCGGAGAt                                                                                                                                                                                           >  2:591495/1‑250 (MQ=255)                                                             ccTGACGAACTGCTGGCGCTGGTTGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAATGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCt                                                                                                                                                                                       <  2:284133/250‑1 (MQ=255)                                                                gACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTaa                                                                                                                                                                                   <  1:653922/251‑1 (MQ=255)                                                                gACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAAAAAAGCTCACCGGTCTTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTAATGCATGAAACAGGTATCCGTACTGTACTGATTCCTTTAGGAAGTCGTGGTGAATGGGCTCGCGTGAATGATGAAGGTCAGCGAGTTCCTGGATTCCGGGTGCAGGCTGTCGCTACCATTGCTGCCGGAGATAACTTTa                                                                                                                                                                                    >  1:461602/1‑250 (MQ=255)                                                                        gctgGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGc                                                                                                                                                                             <  1:727724/249‑1 (MQ=255)                                                                           ggCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTa                                                                                                                                                                         >  2:628557/1‑250 (MQ=255)                                                                             cgcTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATc                                                                                                                                                                      <  1:596118/251‑1 (MQ=255)                                                                              gcTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGCTAATc                                                                                                                                                                      >  1:649309/1‑250 (MQ=255)                                                                                 ggtggACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACg                                                                                                                                                                   <  2:495691/250‑1 (MQ=255)                                                                                  gtggACATTATTACGCCAAACGAAACGGCGGCAGACAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGAACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGc                                                                                                                                                                 >  2:442756/1‑251 (MQ=255)                                                                                    ggACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCa                                                                                                                                                                <  1:585427/250‑1 (MQ=255)                                                                                      aCATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTg                                                                                                                                                             >  1:300383/1‑251 (MQ=255)                                                                                          tattaCGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGGTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTg                                                                                                                                                          <  2:131853/250‑1 (MQ=255)                                                                                             taCGCCAAACGCCACGAAAGCAGAAAAGCTCACCGGTATTCGTGTTGCAAATGATGCAGATGCAACGCCGGCGGCGCAGGAACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGCATGATGAAGGTCAGCGCGTTACTGGATTCCGGGTGCAGGCTGTCGATACCCTTGCTGCCGGAGATACCTTTCACGGTGCGTTACTCAAGGCATTGCTggc                                                                                                                                                        >  2:430315/1‑248 (MQ=255)                                                                                              aCGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGTTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGgaag                                                                                                                                                      <  1:109072/250‑1 (MQ=255)                                                                                              aCGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGgaag                                                                                                                                                      <  1:431220/250‑1 (MQ=255)                                                                                              aCGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTGGAACGGTGCGTTAAGCACGGCATTGCTGgaaga                                                                                                                                                     <  2:333290/251‑1 (MQ=255)                                                                                               cGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTGTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCGGTCTATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGgaagaa                                                                                                                                                    <  1:69526/251‑1 (MQ=255)                                                                                                        aaacgGAAGCAGAAAAGCCCTCCGGTATTCGTGGTGAAAATGATGAAGATGCAACGATGGCGGCTCAGGTACTGCAGGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTg                                                                                                                                           <  2:346107/251‑1 (MQ=255)                                                                                                           cgGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATTCCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCa                                                                                                                                        <  1:308157/251‑1 (MQ=255)                                                                                                            ggAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGcc                                                                                                                                         >  1:93725/1‑249 (MQ=255)                                                                                                             gAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTTGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCaga                                                                                                                                      <  1:30963/251‑1 (MQ=255)                                                                                                             gAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCaga                                                                                                                                      <  1:502240/251‑1 (MQ=255)                                                                                                              aaGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACGGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCagag                                                                                                                                     <  1:442756/251‑1 (MQ=255)                                                                                                                gCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGc                                                                                                                                   <  1:208502/251‑1 (MQ=255)                                                                                                                   gAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCAt                                                                                                                                             >  2:383123/1‑238 (MQ=255)                                                                                                                   gAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCAt                                                                                                                                             <  1:383123/238‑1 (MQ=255)                                                                                                                   gAAAAGCTCACCGGTATTCGTGGTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGAt                                                                                                                                <  2:311499/251‑1 (MQ=255)                                                                                                                      aaGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCg                                                                                                                             >  1:586866/1‑251 (MQ=255)                                                                                                                      aaGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCg                                                                                                                             <  1:235799/251‑1 (MQ=255)                                                                                                                          tCACCGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACAATTGCCAGAGGCGATTCGtttt                                                                                                                         >  1:148012/1‑251 (MQ=255)                                                                                                                              cGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAgg                                                                                                                                    <  2:590640/236‑1 (MQ=255)                                                                                                                              cGGTATTCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAgg                                                                                                                                    >  1:590640/1‑236 (MQ=255)                                                                                                                                    tCGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCAGGGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCAtgct                                                                                                                <  2:663845/250‑1 (MQ=255)                                                                                                                                     cGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCAtgctgc                                                                                                              <  2:486421/251‑1 (MQ=255)                                                                                                                                     cGTGTTGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCAtgctg                                                                                                               <  2:300383/250‑1 (MQ=255)                                                                                                                                          tGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTg                                                                                                         >  2:212522/1‑251 (MQ=255)                                                                                                                                          tGAAAATGATGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTAGTTTTGCCCATGCTGCCGCt                                                                                                          <  1:30142/250‑1 (MQ=255)                                                                                                                                              aaTGAGGAAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGAt                                                                                                     <  2:547441/251‑1 (MQ=255)                                                                                                                                                    gaAGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGt                                                                                               >  1:89576/1‑251 (MQ=255)                                                                                                                                                      aGATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTTTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTa                                                                                              >  1:207519/1‑250 (MQ=255)                                                                                                                                                       gATGCAACGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAac                                                                                            >  2:597600/1‑251 (MQ=255)                                                                                                                                                          gcaacGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAacac                                                                                          <  1:38314/246‑1 (MQ=255)                                                                                                                                                          gcaacGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACg                                                                                         <  1:588314/247‑1 (MQ=255)                                                                                                                                                              cGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTaa                                                                                      >  1:230906/1‑250 (MQ=255)                                                                                                                                                                         cAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGGAACACGTAAAGGCGCACAAcc                                                                          <  1:259662/251‑1 (MQ=255)                                                                                                                                                                          aGGTACTGCATGAAAAAGGTATCCGTATTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCAGTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATGGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCt                                                                         <  2:386832/251‑1 (MQ=255)                                                                                                                                                                                  cATGAAAAAGGTATCCGTACTGTACTTATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCATCGCGTTCCTGGATGCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATTCTGCCGCTGCGATTGCCGTAACACGGAAAGGCGCACAACCTTCCGTAcc                                                                 <  2:730217/251‑1 (MQ=255)                                                                                                                                                                                  cATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTAcc                                                                 <  2:298621/251‑1 (MQ=255)                                                                                                                                                                                                ccGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATGCCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAATAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAagag                                                    <  1:668084/250‑1 (MQ=255)                                                                                                                                                                                                  gtactgtactGCTTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCCCAACCTTCCGTACCGTGGCGTGAag                                                      >  2:678448/1‑246 (MQ=255)                                                                                                                                                                                                  gtactgtactGATTACTTTCGGAAGTCGTGGTGTATGGGCTAGCGTGAATGCTGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTAGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAag                                                      >  1:489988/1‑246 (MQ=255)                                                                                                                                                                                                    actgtactgATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCg                                                >  1:410967/1‑250 (MQ=255)                                                                                                                                                                                                    actgtactgATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCg                                                <  2:538063/250‑1 (MQ=255)                                                                                                                                                                                                      attactgATTACTTTCTGAAGACGAGGGGTATGTACTAGCGTTAATGATGAAGGTCAGCGCGATCCTGGATTCCGGGGGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTCATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTACAGGCGCACAACCTGCCGTACCGTGGCGTGAAGAGATCGa                                               >  2:389385/3‑249 (MQ=255)                                                                                                                                                                                                         actgATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCAt                                          <  2:220775/251‑1 (MQ=255)                                                                                                                                                                                                          ctgATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCAtt                                         >  1:78759/1‑251 (MQ=255)                                                                                                                                                                                                           tgATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATCCCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCAttt                                        >  1:386403/1‑251 (MQ=255)                                                                                                                                                                                                            gATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCAtt                                         <  1:138399/249‑1 (MQ=255)                                                                                                                                                                                                                aCTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGGGGCGTGAAGAGATCGACGCAttttt                                      >  2:530278/1‑248 (MQ=255)                                                                                                                                                                                                                   ttAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGaa                                  >  1:610508/1‑248 (MQ=255)                                                                                                                                                                                                                   ttAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTGCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACa                                 <  2:642798/250‑1 (MQ=255)                                                                                                                                                                                                                         aGTCGTGGTGTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGCCGCATTTTTAGACAGGCag                            >  1:363361/1‑249 (MQ=255)                                                                                                                                                                                                                             gtggtgTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGa                      <  2:89576/251‑1 (MQ=255)                                                                                                                                                                                                                             gtggtgGATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGa                      <  2:438373/251‑1 (MQ=255)                                                                                                                                                                                                                               ggtgTATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACTGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGTATTTTTAGTCAGGCAGAGGTGAc                     <  1:207100/250‑1 (MQ=255)                                                                                                                                                                                                                                 tgtATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCt                  >  1:299287/1‑251 (MQ=255)                                                                                                                                                                                                                                  gtATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCtt                 <  1:628557/251‑1 (MQ=255)                                                                                                                                                                                                                                   tATGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGTTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCACTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGTCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAATATATCTACGCATTGTTATACTGGCAGAGGTGACGCtt                 <  2:651618/250‑1 (MQ=255)                                                                                                                                                                                                                                    aTGGGCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTg                <  2:526040/250‑1 (MQ=255)                                                                                                                                                                                                                                       ggCTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCt             >  1:633513/1‑250 (MQ=255)                                                                                                                                                                                                                                         cTAGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCCTTTTTAGACAGGCAGAGGTGACGCTTGGCTAc           >  2:315048/1‑250 (MQ=255)                                                                                                                                                                                                                                           aGCGTGAATGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAAt        <  1:678448/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aaTGATGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCGTGGCTACAATGAAAGa  <  1:545364/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       AAAATCGCCCATCAAAATAAGACTATCGTTGCGCTTAACCCGGCTCCGGCTCGCGAACTTCCTGACGAACTGCTGGCGCTGGTGGACATTATTACGCCAAACGAAACGGAAGCAGAAAAGCTCACCGGTATTCGTGTTGAAAATGATGAAGATGCAGCGAAGGCGGCGCAGGTACTGCATGAAAAAGGTATCCGTACTGTACTGATTACTTTAGGAAGTCGTGGTGTATGGGCTAGCGTGAATGGTGAAGGTCAGCGCGTTCCTGGATTCCGGGTGCAGGCTGTCGATACCATTGCTGCCGGAGATACCTTTAACGGTGCGTTAATCACGGCATTGCTGGAAGAAAAACCATTGCCAGAGGCGATTCGTTTTGCCCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGA  >  NC_000913/3937753‑3938243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑