Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,581,850	G→A	intergenic (+224/‑13)	yhhY → / → yhhZ	N‑acetyltransferase YhhY/putative endonuclease YhhZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,581,850	0	G	A	100.0%	92.9 / NA	26	intergenic (+224/‑13)	yhhY/yhhZ	N‑acetyltransferase YhhY/putative endonuclease YhhZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/15);  total (11/15)

ATGGCGCGGGTGAAGTAAGATAGTGCCCTTTTTCTGAGATGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAGGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATT  >  NC_000913/3581609‑3582077                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    atGGCGCGGGTGAAGTAAGATAGTGCCCTTTTTCTGAGATGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTc                                                                                                                                                                                                                             <  1:360809/250‑1 (MQ=255)   ggCGCGGGTGAAGTAAGATAGTGCCCTTTTTCTGAGATGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAgg                                                                                                                                                                                                                          <  2:556535/251‑1 (MQ=255)      gcgGGTGAAGTAAGATAGTGCCCTTTTTCTGAGATGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGAt                                                                                                                                                                                                                       <  2:12885/251‑1 (MQ=255)          gTGAAGTAAGCTAGTGCCCTTTTTCTGAGATGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGt                                                                                                                                                                                                                   >  2:302891/1‑251 (MQ=255)                                           caaaaGGGTGTCATTCAAAATCGACATCACTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTACTAAAGTGAAATATTTTATTTTTTAATATATACGCATACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGACATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGaaca                                                                                                                                                                                   >  2:432596/2‑250 (MQ=255)                                            aaaaGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGAATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGaacaac                                                                                                                                                                                 <  1:56042/251‑1 (MQ=255)                                                    gtCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAg                                                                                                                                                                          <  1:521805/250‑1 (MQ=255)                                                        ttCAAACTCGACATACCTTCCTTTAAGGGATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTAATTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGTATGAGTAATATTGTTTACCTGACATTAACGGGAGAACAACAAGGAAGCAtct                                                                                                                                                                     >  2:561919/1‑251 (MQ=255)                                                                                                cAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAAGCGTTg                                                                                                                             <  2:442299/251‑1 (MQ=255)                                                                                                      atatatTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCagag                                                                                                                       <  2:513033/251‑1 (MQ=255)                                                                                                            tGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGc                                                                                                                  <  2:159159/250‑1 (MQ=255)                                                                                                                    aaCTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATg                                                                                                         <  2:275030/251‑1 (MQ=255)                                                                                                                              tACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAATCATAATATTGCCATTGAGAAAATAGCAGGCCATAAAAAGACTGTTCAGGGATTATTAATATTGTTTACCTGACAGTAACGGGATAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACGTCTGAGTCTACAGGTAAGCGTTGGCAGAGCGGGCATGAGGATGATATATTTa                                                                                                <  1:88424/250‑1 (MQ=255)                                                                                                                                  tCCTAAAGTGTAATATTTTATTTTTTAATATATACGACTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATACTATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACATGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTCAAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATtc                                                                                           >  2:445179/1‑251 (MQ=255)                                                                                                                                    cTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATtctc                                                                                         >  1:57823/1‑251 (MQ=255)                                                                                                                                         gtgtAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCActct                                                                                    >  1:683958/1‑251 (MQ=255)                                                                                                                                                       tttttAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATg                                                                                                                                                                                                                      <  1:228495/107‑1 (MQ=255)                                                                                                                                                       tttttAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATg                                                                                                                                                                                                                      >  2:228495/1‑107 (MQ=255)                                                                                                                                                        ttttAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATtaat                                                                      >  1:555970/1‑250 (MQ=255)                                                                                                                                                            aaTATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAAGATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAataata                                                                  <  2:324491/250‑1 (MQ=255)                                                                                                                                                                     gCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTgg                                                        >  2:452677/1‑251 (MQ=255)                                                                                                                                                                               tCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGtt                                               <  2:166486/250‑1 (MQ=255)                                                                                                                                                                                ccTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTcc                                             <  1:662015/251‑1 (MQ=255)                                                                                                                                                                                                       aaCAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTaactt                       >  2:358046/1‑247 (MQ=255)                                                                                                                                                                                                                           tGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGATAACAACAAGGAAGCATCTCCGCAGGTTGTGGGTCTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCat   <  1:445179/250‑1 (MQ=255)                                                                                                                                                                                                                           tGATAAAATAGCATGCCATAAAAAGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCatt  >  1:42437/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    ATGGCGCGGGTGAAGTAAGATAGTGCCCTTTTTCTGAGATGGAAAAAGGGTGTCATTCAAAATCGACATACCTTCCTTTAAGGTATTTATTTGCCCAATACATATATTGAGATTTAACTCATAATTACTTCCTAAAGTGTAATATTTTATTTTTTAATATATACGCCTACAATTTCCTGGAGTAAATAAATAACAATTAACAAGCATAATATTGCCATTGATAAAATAGCATGCCATAAAAGGACTTTTCAGGGATGAGTAATATTGTTTACCTGACAGTAACGGGAGAACAACAAGGAAGCATCTCCGCAGGTTGTGGGACTTCTGAGTCTACAGGTAATCGTTGGCAGAGCGGGCATGAGGATGAAATATTTACATTCTCACTCTTAAATAATATTAATAATACGGGGCTTGGTTCACAGTTCCATGGTATAACATTTTGTAAATTAATTGATAAAAGCACTCCATT  >  NC_000913/3581609‑3582077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑