Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,558,291	C→T	G8G (GGC→GGT)	rtcR →	DNA‑binding transcriptional activator RtcR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,558,291	0	C	T	100.0%	218.7 / NA	62	G8G (GGC→GGT)	rtcR	DNA‑binding transcriptional activator RtcR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/25);  total (37/25)

ATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGC‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAAGAGGTCTACGC  >  NC_000913/3558050‑3558536                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       aTTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACATCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAAAAGTGGCTTTTGGT‑TTTGTCGGt                                                                                                                                                                                                                                              >  2:697005/1‑251 (MQ=255)        gTGGTAAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCATGATAATTGCAACCGTCGTGCCAGAACATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGCTAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTAc                                                                                                                                                                                                                                       >  2:244087/1‑251 (MQ=255)             cAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGAt                                                                                                                                                                                                                                  <  2:167578/251‑1 (MQ=255)              aGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGAtt                                                                                                                                                                                                                                 >  1:137733/1‑251 (MQ=255)                  attcgtaattcATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTACAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAACTCAATGTTTTAAAAAAGATTATCTCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCCATTTACCGATAATAAAATATCGTCTTTTATAAGGATATATAAGATGCGTAAAACAGGGGCTTTTGGT‑TTTGTCGATAACGCACTGGATTATGc                                                                                                                                                                                                                             >  1:443718/1‑251 (MQ=255)                     cgtaattcATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGTCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCgg                                                                                                                                                                                                                          <  1:38329/251‑1 (MQ=255)                     cgtaattcATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCg                                                                                                                                                                                                                           <  1:25238/250‑1 (MQ=255)                        aattcATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAATCAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGgc                                                                                                                                                                                                                        <  1:327489/250‑1 (MQ=255)                         attcATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATACTGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTTTCGGTACCGTACTGGATTATGCCGGgcgc                                                                                                                                                                                                                      >  2:492557/1‑251 (MQ=255)                                ttGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCAGGGCGCGGCAGTc                                                                                                                                                                                                               >  1:625859/1‑251 (MQ=255)                                 tGTTTTTCTCGTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAATATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTc                                                                                                                                                                                                               >  2:236739/1‑250 (MQ=255)                                     tttCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAATTATGCAGATTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAATCCAATTTACCGTTAATGAAATATAGTCTTTTATATGGATATCTACGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAgcgt                                                                                                                                                                                                          >  2:8431/1‑250 (MQ=255)                                               cGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCaaaa                                                                                                                                                                                                 <  1:610218/250‑1 (MQ=255)                                                      tGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCACAATGGCGTcc                                                                                                                                                                                         >  1:174848/1‑251 (MQ=255)                                                       gCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑GTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTcc                                                                                                                                                                                         <  2:55591/250‑1 (MQ=255)                                                             cTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACActc                                                                                                                                                                                  >  1:346596/1‑251 (MQ=255)                                                                  aaTTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGttt                                                                                                                                                                             >  2:105676/1‑251 (MQ=255)                                                                  aaTTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGtt                                                                                                                                                                              <  1:475664/250‑1 (MQ=255)                                                                        aaCCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGcc                                                                                                                                                                        >  2:81305/1‑250 (MQ=255)                                                                                          ttAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCat                                                                                                                                                     >  1:170871/1‑251 (MQ=255)                                                                                           tAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCACAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCatc                                                                                                                                                    >  1:43579/1‑251 (MQ=255)                                                                                              aTAAGCAGCTTAATTTTTTAAATCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGATTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCatcgat                                                                                                                                                 >  1:508293/1‑251 (MQ=255)                                                                                                   cagcTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACt                                                                                                                                            >  1:544442/1‑251 (MQ=255)                                                                                                             ttttAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTAATCGATCGACTGGAAttgttg                                                                                                                                  >  2:413451/1‑251 (MQ=255)                                                                                                              tttAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTACCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAAttgttg                                                                                                                                  <  2:170871/250‑1 (MQ=255)                                                                                                                      aTTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGcc                                                                                                                         <  1:33663/251‑1 (MQ=255)                                                                                                                              aaaaaaGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGGCACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgc                                                                                                                  <  1:492557/250‑1 (MQ=255)                                                                                                                              aaaaaaGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTGTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgc                                                                                                                  <  1:656694/250‑1 (MQ=255)                                                                                                                                aaaaGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgcgct                                                                                                               <  1:467775/251‑1 (MQ=255)                                                                                                                                aaaaGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgcgct                                                                                                               >  1:323271/1‑251 (MQ=255)                                                                                                                                  aaGATTATATCTTTTCGTACGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCcgctcgcgctcg                                                                                                             <  1:163549/251‑1 (MQ=255)                                                                                                                                         tatCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTg                                                                                                      >  2:293354/1‑251 (MQ=255)                                                                                                                                            cTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGaa                                                                                                    >  2:227871/1‑250 (MQ=255)                                                                                                                                              gtACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGAGGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAATAATCGTTGTTCATCGATCGACTGGAATTGTTGCACGACGCCCGCGCGCGCTCGCTATTTGAAACg                                                                                                 <  2:123976/250‑1 (MQ=255)                                                                                                                                               tACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGAGTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATTCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACg                                                                                                 <  1:424605/250‑1 (MQ=255)                                                                                                                                               tACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATATAAGATGCGTAACACCGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAACTGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTTTTTGAAACGc                                                                                                >  1:380683/1‑251 (MQ=255)                                                                                                                                                aCGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TGTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACTCTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGc                                                                                                <  1:484704/250‑1 (MQ=255)                                                                                                                                                   tCCGTAACCGAAGCTTTCCCGAAAAGCCAATTTACCGATAATGACCTATCGTATTTTATAAGGATATCTAAGATGCGTAAACCAGTGGCTTTTGGT‑TTTGTCGGTACCGCACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACTCTCTGTTTATGCCAGCCAGAATAGTTGGTCATCGATCGCCTGGACTTGTTGCACGCCGCCCGCTCGCGCTCGCTATCTGCCACGCTTa                                                                                             >  2:342936/1‑250 (MQ=255)                                                                                                                                                     cGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAAc                                                                                          >  1:116593/1‑251 (MQ=255)                                                                                                                                                     cGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAAc                                                                                          <  1:48512/251‑1 (MQ=255)                                                                                                                                                      gTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAAcg                                                                                         >  2:221683/1‑251 (MQ=255)                                                                                                                                                                     ccGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTAGGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGt                                                                          <  2:43579/251‑1 (MQ=255)                                                                                                                                                                       gCAAAGCCAATTTACCGATATTGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGttt                                                                        >  2:71400/1‑251 (MQ=255)                                                                                                                                                                            gCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGccc                                                                   >  2:440538/1‑250 (MQ=255)                                                                                                                                                                                            tGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAgtggtg                                                    >  2:10570/1‑250 (MQ=255)                                                                                                                                                                                             gAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGa                                                   <  1:293354/250‑1 (MQ=255)                                                                                                                                                                                             gAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAg                                                  <  1:414423/251‑1 (MQ=255)                                                                                                                                                                                             gAAATATCGTCCTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGCCACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCCGAAACAGaaaaggttgg                                                  >  2:96460/1‑243 (MQ=255)                                                                                                                                                                                                  atCGTCTTTTATAAGGATATCTAAGATGCGTACATCAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGTCACTCTGTTTATGCCAGCAAGAATCGTTGGTAATCGATCGACTGGCATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGCAACCGAAGTGGTGAGCGTTg                                             >  2:465841/1‑251 (MQ=255)                                                                                                                                                                                                   tCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTg                                             >  2:235334/1‑250 (MQ=255)                                                                                                                                                                                                     gTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTgaga                                          <  2:323271/251‑1 (MQ=255)                                                                                                                                                                                                         tttATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAACCAGAAGTGGTGAGCGTTGCGATTg                                       >  2:678664/1‑250 (MQ=255)                                                                                                                                                                                                            ataAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACt                                   >  1:412477/1‑251 (MQ=255)                                                                                                                                                                                                                 gATATCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATa                              <  1:71400/251‑1 (MQ=255)                                                                                                                                                                                                                  atatCTAAGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATa                              >  2:512570/1‑250 (MQ=255)                                                                                                                                                                                                                        aaGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTg                       <  1:96460/251‑1 (MQ=255)                                                                                                                                                                                                                         aGATGCGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTgg                      >  2:341725/1‑251 (MQ=255)                                                                                                                                                                                                                              cGTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGAttt                 >  2:600222/1‑251 (MQ=255)                                                                                                                                                                                                                               gTAAAACAGTGGCTTTTGGT‑TTTGTCGGTACCGTCCTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGACGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTc                >  1:180249/1‑251 (MQ=255)                                                                                                                                                                                                                                  aaaCAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGaa             <  1:221683/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaCAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGGCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGc                                                 <  1:673060/214‑1 (MQ=255)                                                                                                                                                                                                                                   aaCAGTGGCTTTTGGT‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGGCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGc                                                 >  2:673060/1‑214 (MQ=255)                                                                                                                                                                                                                                     acagaccctttggccttTGTCGGTACCGTACTGGCTTATGCCGAGCCCGGCAGTCAGCGCTGGTCAAAATGGCGTCCAACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATAGACTGGACTTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAAAGCTTAACCGCGATATCGCCAGCGTTTCGCCAGACACAGACGCGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGa              >  2:106529/9‑248 (MQ=255)                                                                                                                                                                                                                                        tggcttttggt‑tttGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAAGAGGTc       >  1:132030/1‑251 (MQ=255)                                                                                                                                                                                                                                          gcttttggt‑tttGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAAGAGGTCTa     <  2:346596/251‑1 (MQ=255)                                                                                                                                                                                                                                             tttggt‑tttGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAAGAGGTCTACgc  >  1:88848/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       ATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAATTCATTGTTTTAAAAAAGATTATATCTTTACGTCCGTAACCGGAGATTTCCCGCAAAGCCAATTTACCGATAATGAAATATCGTCTTTTATAAGGATATCTAAGATGCGTAAAACAGTGGCTTTTGGC‑TTTGTCGGTACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGCTGGTCAAAATGGCGTCCGACACTCTGTTTATGCCAGCAAGAATCGTTGGTCATCGATCGACTGGAATTGTTGCACGACGCCCGCTCGCGCTCGCTATTTGAAACGCTTAAACGCGATATCGCCAGCGTTTCGCCAGAAACAGAAGTGGTGAGCGTTGAGATTGAACTGCATAACCCGTGGGATTTCGAAGAGGTCTACGC  >  NC_000913/3558050‑3558536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑