Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	502,232	G→A	T336I (ACC→ATC)	ybaL ←	putative transporter YbaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	502,232	0	G	A	96.9%	208.1 / NA	64	T336I (ACC→ATC)	ybaL	putative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (36/26);  minor base C (2/0);  total (38/26)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACG  >  NC_000913/501989‑502478                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         cAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGc                                                                                                                                                                                                                                                   >  1:416123/1‑251 (MQ=255) cAAATATCCACTGGGATCAGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCTGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCACCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCCTTCCCAGTCCCGCCAGGATAAACGCGCACTCACCAATCTGCGCCAGGCTGGCGGCGAGGATTAATGc                                                                                                                                                                                                                                                   >  2:58087/1‑251 (MQ=255)  aaaTATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATCTTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCATGCGGCAGTAAATTCCATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGcc                                                                                                                                                                                                                                                  >  2:618068/1‑251 (MQ=255)        ccACTGGGATCTGATTCTCTTCTTCGCTTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGt                                                                                                                                                                                                                                            >  2:703540/1‑251 (MQ=255)             gggATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTggg                                                                                                                                                                                                                                        >  2:118548/1‑250 (MQ=255)                      ttctcttctTCGATTGCATCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCCGATATTTCTCCAGTAGTG‑CGAACAGTCCCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGACTGGCGGCGCTGCTTAATGCCGTACGATGGGAGTGACCaa                                                                                                                                                                                                                               >  2:410668/1‑250 (MQ=255)                          cttcttCAATTCCCTCTCCCAGCGTCTGCTCTTCCAGCGTTGCGGTCTTCGCCAGATATTTCTCCAGTATTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGTGAGGGACCAAACa                                                                                                                                                                                                                            <  2:375948/249‑1 (MQ=255)                            tcttctATGGCCTCTTCCAGCTTCGGCTCTTCCAGCGTTGCGGTCTCCGCCAGATATTGCTCCAGTAGAG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGCCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑Gt                                                                                                                                                                                                                         <  1:246468/249‑1 (MQ=255)                                      cctcctCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGAACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCACTACCAGTCCCGCCAGGATAAACGCGCACTCACCCATATGCACCCCGCTGGCGGCGATGATTAATGCCGGACGTTGGGCGTGACCAA‑CACGTCGCACCAggca                                                                                                                                                                                                              >  2:293505/1‑248 (MQ=255)                                            ccAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCg                                                                                                                                                                                                       >  1:683528/1‑251 (MQ=255)                                            acAGCGTCTGCTCTTCCAGCGTTGCGGTTTGCGCCAGATATTTCGCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTTCCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGc                                                                                                                                                                                                        <  1:90542/249‑1 (MQ=255)                                               gCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCt                                                                                                                                                                                                    <  1:195615/251‑1 (MQ=255)                                                   cTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGCCAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCTGATTCATGCCGTACGTTGGGAGTGACCAAACA‑GGCGCACCAGGAAAAATGCGACTAACg                                                                                                                                                                                                >  1:512351/1‑251 (MQ=255)                                                       tctTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACtt                                                                                                                                                                                            >  1:168069/1‑251 (MQ=255)                                                          cccAGCGTTCCGTTCGTCGCCAGATATTTCTCCA‑TTGTGGCGAACAGTACCGGGTTGGGCATAATCGACAGGATCTCCCCAGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATCCAATGCCATTCCCAGTCCCGCCAGGAGAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCTATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTAcc                                                                                                                                                                                         <  2:711783/250‑1 (MQ=255)                                                           ccAGCGTTTCGGGCTTCGCCAGAGATTGCTCCATTAGGG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGGCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTGGGTAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGTTCACGAGTTACCa                                                                                                                                                                                        <  2:99120/251‑1 (MQ=255)                                                                gTTTCGATCTTCACCAGATATTTCTCCAGTAGTG‑CGCACAGTACCGGGTTGAGCCTCATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCCGTACATTCAATGCCATTCCCAGTCCCGCCCGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCTGATTAATGCCGTACGTTGGGAGTGACCAACCC‑GTCGCACCAGGAACAATGCGGCTAACGACTTACCacacgg                                                                                                                                                                                   >  2:351233/1‑246 (MQ=255)                                                                 tttCGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAAc                                                                                                                                                                                     >  1:233265/1‑248 (MQ=255)                                                                    cGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaat                                                                                                                                                                                >  2:104080/1‑250 (MQ=255)                                                                      gTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GGCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGaataat                                                                                                                                                                             >  2:395703/1‑251 (MQ=255)                                                                              cAGATATTTCCCCAGTAGGG‑CGAACGTTCCCAGGTGAACCATAAGCGACAGGACCGCCCCGGCCAGTACCAGGTTTTGCCCGGCCTGCGTCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAATCGCGAACTCCCCAAGCGGCGCCAGTCTGGCGGCGATGATGAATGCCGTTCTTTGGGATTGACCAAACA‑GTCGCACCAGGAAAAAGGCGGCTAACGACTTCCCACACAGAATAAGCGCCAGCg                                                                                                                                                                     <  2:676891/251‑1 (MQ=255)                                                                                          cAGTAGTG‑CGACCAGTCCCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCCGTCCCGCCAGGATACACGCGAACTCACCAATCTGAGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAACCCGAATAATCGCCAGCGTCGCCAGCACTg                                                                                                                                                         >  2:380116/1‑251 (MQ=255)                                                                                              agtG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCa                                                                                                                                                      <  1:75144/250‑1 (MQ=255)                                                                                               gtG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTTCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGc                                                                                                                                                    <  1:472984/251‑1 (MQ=255)                                                                                                 g‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCg                                                                                                                                                   <  1:462695/250‑1 (MQ=255)                                                                                                   cGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGt                                                                                                                                                 >  1:487683/1‑251 (MQ=255)                                                                                                     aaCAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTg                                                                                                                                               >  2:434314/1‑251 (MQ=255)                                                                                                        agtactggttggAGCATAACCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATCCACAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGTCGGCGATGATTAATGCCGGACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGATGCTg                                                                                                                                            <  2:317815/240‑1 (MQ=255)                                                                                                          tACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACCGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGCTGCTGa                                                                                                                                           >  2:160380/1‑250 (MQ=255)                                                                                                              gggtgtcgcaaatccaaCAGGATCGTCCCTGCCAGTACCCGGTTTTGTCCGGCCTGCGGCATTAAATTCAAGGCCATTCCCCGGCCCGCCAGGATAAACGCGAACGCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCCGCACTGCCAGCGGTTGCTGAATCAg                                                                                                                                      <  2:403855/235‑1 (MQ=255)                                                                                                              gggTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATc                                                                                                                                        >  1:38847/1‑249 (MQ=255)                                                                                                               ggTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCAAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATc                                                                                                                                        >  1:720498/1‑248 (MQ=255)                                                                                                                    aGCATAATCGACAGGACCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTaa                                                                                                                                <  1:289316/251‑1 (MQ=255)                                                                                                                             gACAGGCTCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGACCaa                                                                                                                        >  2:17426/1‑250 (MQ=255)                                                                                                                                         cctcccAGTCCCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGAc                                                                                                           <  2:416123/247‑1 (MQ=255)                                                                                                                                          cTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACg                                                                                                          >  1:143009/1‑251 (MQ=255)                                                                                                                                           tGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCACACAGAATAATCGCCAGCGTCGCCAGCCCTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACgg                                                                                                         >  1:733119/1‑251 (MQ=255)                                                                                                                                             ccAGTACCAGGTTGTGCCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCTGTACGTTGGGAGTGACCAATCA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGgag                                                                                                       <  1:58087/251‑1 (MQ=255)                                                                                                                                              cAGTACCAGGGTTTGTCCGGCCTGCGGCAGTAACTTAAATGCCATTCCCCGTCCCGCCAGGATAAAAGCGAACTCACCAATCTGCCCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCCAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAACCAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCCGAATCAGAATTAACGGATAAAACAACATCCCGACgg                                                                                                         >  2:707975/1‑248 (MQ=255)                                                                                                                                                 tACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGCTTAATGCCGTACGTTGGGAGTGACCAAACA‑GCCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACa                                                                                                    >  1:337478/1‑250 (MQ=255)                                                                                                                                                  aCCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACaaa                                                                                                  <  1:434314/251‑1 (MQ=255)                                                                                                                                                  aCCAGGTTTTGTCCGGCCTGCGGCAGAAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGACCTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTACCGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGACTCAGAATTAACGGCTCAAACAACATCCCGACGGAGACaa                                                                                                   >  2:660363/1‑250 (MQ=255)                                                                                                                                                            gTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCAcc                                                                                        >  2:609530/1‑251 (MQ=255)                                                                                                                                                                ggCCTGCGGCAGTAAATTCACTGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCa                                                                                     >  2:122353/1‑250 (MQ=255)                                                                                                                                                                ggCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTAGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAACACCGCa                                                                                     >  1:564884/1‑250 (MQ=255)                                                                                                                                                                            tAAATTCAATGCCATTCCCAGTCCCGCCAGGATAACCGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCACCAGCACTGCCAGCGGTTGCTGATTCAGAATTAACGGATCAACCAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AAAGCGTCgcgc                                                                        >  2:160282/1‑250 (MQ=255)                                                                                                                                                                                        cATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTAt                                                           >  2:182722/1‑251 (MQ=255)                                                                                                                                                                                                   ccGCCAGGATAACCACGAACTCACCAAACTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAAAGCGGCTAACGAAAAACCAAACAGAATAATCACCAGCGTCGCCAGCACTGCCAGCGATTGCTAAATCAGAATTAACGGATCAAACAACATCACGACGGAGGCAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGAAGCGAATCGGGggcggc                                                 >  2:681097/1‑250 (MQ=255)                                                                                                                                                                                                     gCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAAGCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACg                                              <  1:703540/251‑1 (MQ=255)                                                                                                                                                                                                       cAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGTGTGGCCAAACA‑GCCGCCCCTGGACAAATGCGGCTACCGACTTACCAAAAAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGCATCAGTATTAACGGATAAAACAACATCCCGACGGAGCCAAAAAACAGCACCGCA‑AACACGTCGCGCAATTGCAGCGTATCGTGGGCGGCACGGt                                            >  1:693421/1‑251 (MQ=255)                                                                                                                                                                                                              aaCGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAg                                     <  1:618068/251‑1 (MQ=255)                                                                                                                                                                                                              aaCGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAg                                     <  2:411844/251‑1 (MQ=255)                                                                                                                                                                                                                  cgAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAACCAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCa                                 <  2:400988/251‑1 (MQ=255)                                                                                                                                                                                                                  cgAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCa                                 <  2:233265/251‑1 (MQ=255)                                                                                                                                                                                                                      cTCACCAATCTGCGCCAGGCTGGCGGCGCTGATTAATGCCGTCCGTTGGGAGTGACCACACA‑GTCGCACCCGGAAAAATGCGGCTAACGACTTCCCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGACTTAACGGATCACACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACt                             >  2:706385/1‑251 (MQ=255)                                                                                                                                                                                                                           cAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACTCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTc                        <  2:168069/251‑1 (MQ=255)                                                                                                                                                                                                                            aaTCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTTGGAGTGACCAAACA‑GCCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACTCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACTGTGACTCAGTTCAGACTCGTTCa                       <  1:204071/251‑1 (MQ=255)                                                                                                                                                                                                                            aaTCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCa                       <  2:38847/251‑1 (MQ=255)                                                                                                                                                                                                                                   gcCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCAt                >  2:663239/1‑251 (MQ=255)                                                                                                                                                                                                                                        gctggctgCGATGATTAATGCCGTACGATGGGAATGACCAAACA‑GTCGCACCAGGAAAAATGCGGGTACCGCATTACCAAACAGAATAATCACCAGCTTCGCCAGCAGTGCCAGCGGTTACTGCATCAGTATTAACGGATCCAACAAGATCCAGACGGAGACAAAAAACAGCACC‑CACAACGCGTCGCGCAATGGCCGCGTATCGTGGGCGGCACGGTGCCTCCATTCAGACTCGATCAGTACCCTCCCgg           >  1:731585/1‑251 (MQ=255)                                                                                                                                                                                                                                          tGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATACTCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGc          >  2:339371/1‑250 (MQ=255)                                                                                                                                                                                                                                           ggcggcGATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCaa        <  2:236224/251‑1 (MQ=255)                                                                                                                                                                                                                                              ggcgATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCaa        >  2:412913/1‑248 (MQ=255)                                                                                                                                                                                                                                                cgATGATTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAAc   <  2:683528/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gatgatTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACg  >  2:27576/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         CAAATATCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTG‑CGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACA‑GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCA‑AACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACG  >  NC_000913/501989‑502478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑