Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,275,902	A→T	E207D (GAA→GAT)	garD →	GarD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,275,902	0	A	T	96.6%	290.4 / NA	89	E207D (GAA→GAT)	garD	GarD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (33/53);  minor base G (1/2);  total (34/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGGCAGCGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGTTGGTATGCAGTGCGG  >  NC_000913/3275660‑3276147                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      gATGGCAGCGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGGTTTACGC‑ACCGGCGGCCGTTTTACCCATCCGTACCATTCACAATATTTTGCTGAATCCTAACTTTTGCGGCGATGTAATggg                                                                                                                                                                                                                                               >  2:120348/1‑250 (MQ=255)      cAGCGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTg                                                                                                                                                                                                                                          <  2:380764/251‑1 (MQ=255)             ggCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTggg                                                                                                                                                                                                                                   <  1:83920/251‑1 (MQ=255)              gCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGt                                                                                                                                                                                                                                  <  1:189812/251‑1 (MQ=255)                  cAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTGCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTtgtg                                                                                                                                                                                                                              <  2:76470/251‑1 (MQ=255)                       aCCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGGTTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAAAGGTGATTGGCCTGGGTTGTGaaa                                                                                                                                                                                                                           >  1:515321/1‑249 (MQ=255)                       aCCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCCGGTGTACCTATCCGTACCATTCCCAATATTTCGCTGAATCCTAACTTTGGCGGCGGTGTAATGGTGATTGGCCTTGGGTGTGgaaa                                                                                                                                                                                                                          >  1:588662/1‑250 (MQ=255)                           gCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTg                                                                                                                                                                                                                      <  1:244854/250‑1 (MQ=255)                            cTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTTGCTTAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGc                                                                                                                                                                                                                     >  1:187399/1‑250 (MQ=255)                              cGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGc                                                                                                                                                                                                                  >  1:134899/1‑251 (MQ=255)                               ggTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGcc                                                                                                                                                                                                                 <  2:588662/251‑1 (MQ=255)                               ggTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTAACATTCCCAATATTTCGCTGAATCCTAACTTTGGCGGGGAGGTAATGGTGATTGGCCTGGGTTGTGAAAAGGTGTAGcc                                                                                                                                                                                                                 >  1:120628/1‑251 (MQ=255)                                   tCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGCAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGCTGTAACAATCCGTACCATTCACAATATTTCGCTGAAACCTAACTTTGGCGGCGTTGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAACCta                                                                                                                                                                                                               >  1:337460/1‑248 (MQ=255)                                              gTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGAc                                                                                                                                                                                                  <  1:523424/251‑1 (MQ=255)                                                ccACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTCCCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGGTTCACGCCACCG‑CGGCAGTTTTACCTATTCGGTCCCTTCACAATATTTCGCTGAAACCTCACTTTGGTGGGGGTGTAATGGTGATTGGGCTGGGTTGTGAAAAGATTCCGGCTGAGCGCcttatggctg                                                                                                                                                                                                >  2:688556/1‑243 (MQ=255)                                                ccACGGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACt                                                                                                                                                                                                 <  2:661019/250‑1 (MQ=255)                                                    tgtgtgGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGGGATTAACGC‑ACCGGCGGCAGTTGTACCTATTCCTACCATTTACAATATTTCGCTGAATCCTAACTTTGGCGGCGGTGTAATGGTGATTTGGCTGGGGTGTGAAAAGTTGCCACCTGAGCGCCCGCAGGCTGGaa                                                                                                                                                                                             >  1:501487/1‑250 (MQ=255)                                                     gggtgGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATTCCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTTCCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGaa                                                                                                                                                                                             <  1:520800/247‑1 (MQ=255)                                                       gtgGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGga                                                                                                                                                                                         <  1:531189/251‑1 (MQ=255)                                                        gggCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGgat                                                                                                                                                                                        <  2:1105/250‑1 (MQ=255)                                                         ggCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGgat                                                                                                                                                                                        <  1:514409/250‑1 (MQ=255)                                                               cGTGGTGGACTATGTAGTAAAAATCAGTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGTCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGTCGGCAGTTGTACCGATGCGTACCATTCACAATATTTCGCTGAATCCTAACTTGGGCTTCGATGTAATGGTGATTGGCTTGGGTTATGAAAAGCTGCATCCTGAGCGCCGGCTGACTGGAACGGAGGATGTGc                                                                                                                                                                                 <  2:65752/251‑1 (MQ=255)                                                                  ggtggACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAg                                                                                                                                                                              <  1:341726/251‑1 (MQ=255)                                                                  ggtggACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAg                                                                                                                                                                              <  1:80590/251‑1 (MQ=255)                                                                      gACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGGCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTGGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTAt                                                                                                                                                                          <  1:494236/251‑1 (MQ=255)                                                                          aTGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTc                                                                                                                                                                        <  1:407612/249‑1 (MQ=255)                                                                          aTGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTc                                                                                                                                                                        <  2:505714/249‑1 (MQ=255)                                                                            gtagtaAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTTGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAAGATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTGGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGt                                                                                                                                                                    <  1:675664/251‑1 (MQ=255)                                                                                     aTCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGAGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGGGATGTAATGGTGATTGGCCTGGGTTGTGGAAAGTTGCAGCCGGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAgcgc                                                                                                                                                           >  1:87751/1‑251 (MQ=255)                                                                                        aTTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAg                                                                                                                                                        <  2:134899/251‑1 (MQ=255)                                                                                           gAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAAACCTAACTTTGGCGGCGCTGTAATGGTGATTGGGCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGGGGAAGCTATTCCCGTAGAAAGCGCCAGCAt                                                                                                                                                     >  2:610296/1‑251 (MQ=255)                                                                                               gcgATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCCTTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGAGGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTc                                                                                                                                                 >  1:133546/1‑251 (MQ=255)                                                                                                cgATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGGTTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAAAATTTCGCCGAATCCTTACTTTGGCGGCGATGTAATGGGGATTGGCCTGGGTTGTGAAAAGTTTCAACCTGAGCGCCTGCTGACTGGAACGGGTGATGGGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTcc                                                                                                                                                >  1:616345/1‑250 (MQ=255)                                                                                                        tACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCCGTTGTACCCATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGGGAAAAGTTTCAGCCCTAGCCCCTGCTGGCTGGGACGGGTGATGGGGCAGCTATTCCCGTAGAAAGCCGCAGCCTTGTCCGttcgatt                                                                                                                                        >  2:173730/1‑246 (MQ=255)                                                                                                        tACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGtt                                                                                                                                             <  1:173730/246‑1 (MQ=255)                                                                                                         aCCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCa                                                                                                                                         <  2:731404/249‑1 (MQ=255)                                                                                                          ccGAAATCCCCGAACGTCTATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGa                                                                                                                                      <  2:30940/251‑1 (MQ=255)                                                                                                          ccGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGa                                                                                                                                      <  1:725265/251‑1 (MQ=255)                                                                                                                    cGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCAt                                                                                                                            <  1:4558/251‑1 (MQ=255)                                                                                                                    cGAACGGCGATGGCGTGGTGGTGCTGAAGCATTTGTACGGGTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCGAACGTTGGCGGCGAGGTAATGGGGATTGGCCTGGGTTGTGAAAAGTGGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCAt                                                                                                                            <  2:286503/251‑1 (MQ=255)                                                                                                                      aaCGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCCGTTGTACCTATCCGTACCATTCCCAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGGTGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTTTCAGTTTGCAGGATGAAAAGCATGt                                                                                                                          >  1:85922/1‑251 (MQ=255)                                                                                                                      aaCGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGt                                                                                                                          <  1:120348/251‑1 (MQ=255)                                                                                                                       aCGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTc                                                                                                                         <  2:347403/251‑1 (MQ=255)                                                                                                                           cGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTGGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCGGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGt                                                                                                                      <  2:673187/250‑1 (MQ=255)                                                                                                                            gATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATGCACAATATTTCGCTGAATCCTAACTGTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATGCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGGTGAAAAGCATGTCGGttt                                                                                                                    <  1:248029/251‑1 (MQ=255)                                                                                                                                 gggggtggGGCTGAATCATTTGTACGGTTGGGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGt                                                                                                               <  2:198055/248‑1 (MQ=255)                                                                                                                                 cGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGt                                                                                                               <  1:616433/251‑1 (MQ=255)                                                                                                                                  gtggtgGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTCTCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGGTTGGCCTGGGTTGTGAAAAGTTGTAGCCTGAGCGCCTGCTGACTGGAACGGATGGTGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTTTCAGTTTGCAGGGTGAAAAGCATGTCGGGTTTTAGTc                                                                                                              >  2:206283/1‑251 (MQ=255)                                                                                                                                  gtggtgGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGGTGTACCTATTCGTACCATTCACAATATTTCGCTGAATCCTAAATTTGGCGGCGGTGTAATGGTGATTGGGCTGGGGTGTGGAAAGTTTCAGCCTGAGGGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGtttttcgtc                                                                                                              >  2:614587/1‑246 (MQ=255)                                                                                                                                       ggggCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGt                                                                                                                          <  2:430613/234‑1 (MQ=255)                                                                                                                                       ggggCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCAGAGGCAGTTGTACCTATCCGTCCCATTCACACTATTTTGCTGAATTCCAACTTTGGCGGCGATGTAATGGGGATTGGCCCGGGGTGTGAAAAGTTACAGCCTGAGCGCCCGCTGACTGGAACGGGTGATGTGCAAGCTATTTCAGTAGAAAGCGCCAGCCTTGTCCGTTTGCAGGATGGAAAGCATGTAGGtcgggagagcacac                                                                                                         >  1:430613/1‑238 (MQ=255)                                                                                                                                        gggCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGAGGAAAAGCATGTCGGTTTTCAGTCCATGGt                                                                                                        <  1:160714/251‑1 (MQ=255)                                                                                                                                           cTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGGTGTACCTATCCGTACCATTCACAATATTTCGCTGAAACCTAACTTTGGCGGCGATGTAATGGTGGTTGGCCTGGGGTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGGTGATGTGCCAGCTATTCCAGTAGAAAGAGACAGCCTTGTCAGTTTGCAGGGTGAAAAGCATGTCGGTTTTCAGTCCATGGTCgg                                                                                                     >  1:637219/1‑250 (MQ=255)                                                                                                                                              aaTCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGa                                                                                                  <  2:600880/251‑1 (MQ=255)                                                                                                                                              aaTCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGa                                                                                                  <  1:638932/251‑1 (MQ=255)                                                                                                                                                 cATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGatat                                                                                               <  1:493889/251‑1 (MQ=255)                                                                                                                                                     tGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTGTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATAtttt                                                                                            <  2:608383/250‑1 (MQ=255)                                                                                                                                                              gtggcgtggcGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGcc                                                                                  <  1:708689/251‑1 (MQ=255)                                                                                                                                                                ggcgtggcgATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCg                                                                                 >  1:690777/1‑250 (MQ=255)                                                                                                                                                                  cgtgtctaTTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCAATCACAATATTTCGCTGAATCCTAACTTAGGCGGCGAGGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTGGCAGATCGCCGAAc                                                                              <  1:512604/244‑1 (MQ=255)                                                                                                                                                                   gtggcgATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACg                                                                             <  2:85922/251‑1 (MQ=255)                                                                                                                                                                     ggcgATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGAGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGcc                                                                           >  2:427420/1‑251 (MQ=255)                                                                                                                                                                         aTTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCGTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCt                                                                       <  1:21264/251‑1 (MQ=255)                                                                                                                                                                               gC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCATAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACaaa                                                                  >  1:692045/1‑250 (MQ=255)                                                                                                                                                                                c‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGa                                                                                                  <  1:491516/217‑1 (MQ=255)                                                                                                                                                                                c‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGa                                                                                                  >  2:491516/1‑217 (MQ=255)                                                                                                                                                                                   ccGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCg                                                                                                      <  2:588813/211‑1 (MQ=255)                                                                                                                                                                                   ccGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCg                                                                                                      >  1:588813/1‑211 (MQ=255)                                                                                                                                                                                   ccGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACt                                                              >  1:727895/1‑251 (MQ=255)                                                                                                                                                                                      gcggcAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGaa                                                           <  1:531791/251‑1 (MQ=255)                                                                                                                                                                                           aGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTCTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAAc                                                      <  2:536728/251‑1 (MQ=255)                                                                                                                                                                                              tGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGc                                                   >  1:381856/1‑251 (MQ=255)                                                                                                                                                                                               gTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGc                                                   >  1:617404/1‑250 (MQ=255)                                                                                                                                                                                               gTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGc                                                   <  2:616345/250‑1 (MQ=255)                                                                                                                                                                                               gTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCa                                                  <  2:187399/251‑1 (MQ=255)                                                                                                                                                                                                     aTCCGTACCACTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTCATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTACCTGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTCCAAAAACTGAATCAACGGCAGCgagc                                            >  2:695851/1‑250 (MQ=255)                                                                                                                                                                                                       ccGTACCCTTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGaa                                           >  2:61354/1‑250 (MQ=255)                                                                                                                                                                                                       ccGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGaaa                                          <  1:394303/251‑1 (MQ=255)                                                                                                                                                                                                           aCCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTGTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCt                                       <  1:688556/250‑1 (MQ=255)                                                                                                                                                                                                             cATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGcc                                    <  1:206283/251‑1 (MQ=255)                                                                                                                                                                                                             cATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACGGAATCAACGGCAGCGAGAAACCTGct                                    <  2:98219/251‑2 (MQ=255)                                                                                                                                                                                                              aTTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGccc                                   <  1:614587/251‑1 (MQ=255)                                                                                                                                                                                                                     atatTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTc                             >  1:672849/1‑250 (MQ=255)                                                                                                                                                                                                                       atTTCGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTc                             >  2:21566/1‑248 (MQ=255)                                                                                                                                                                                                                           cGCTGAATCCTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACt                       <  2:29546/250‑1 (MQ=255)                                                                                                                                                                                                                                   ccTAACTTTGGCGGCGATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGTTgg              >  1:271497/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaCTTTGGCGGCGATGTAATGGTGACTGGACTGGGTTGTGACAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGCTGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAAACTGCCCGGCTTCAGAACTGGTCGTTGTTAt           >  2:60582/1‑251 (MQ=255)                                                                                                                                                                                                                                              cggcgATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGTTGGTATGCAGTGCg   <  2:271497/251‑1 (MQ=255)                                                                                                                                                                                                                                               ggcgATGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGTTGGTATGCAGTGCgg  >  1:110826/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GATGGCAGCGTGGGCACCAAAAACCTGCTCGGTATCACCACCAGCGTCCACTGTGTGGCAGGCGTGGTGGACTATGTAGTAAAAATCATTGAACGCGATCTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGC‑ACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGAGCGCCTGCTGACTGGAACGGATGATGTGCAAGCTATTCCAGTAGAAAGCGCCAGCATTGTCAGTTTGCAGGATGAAAAGCATGTCGGTTTTCAGTCCATGGTCGAGGATATTTTGCAGATCGCCGAACGCCATCTACAAAAACTGAATCAACGGCAGCGAGAAACCTGCCCGGCTTCAGAACTGGTCGTTGGTATGCAGTGCGG  >  NC_000913/3275660‑3276147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑