Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,270,497	C→T	noncoding (96/377 nt)	rnpB ←	RNase P catalytic RNA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,497	0	C	T	100.0%	159.6 / NA	50	noncoding (96/377 nt)	rnpB	RNase P catalytic RNA component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/31);  total (19/31)

AGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCAATACG  >  NC_000913/3270254‑3270742                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        aGTCATTCATCTAGGCCAGCAATCGCTCACGGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCAc                                                                                                                                                                                                                                                  <  2:40073/251‑1 (MQ=255)                                              ccccGGGTTCAGTACGGGCCGTACCTTAAGAACCCCTATTTTGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTccc                                                                                                                                                                                                    <  2:151844/250‑1 (MQ=255)                                                                  gTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGaa                                                                                                                                                                                <  1:472924/251‑1 (MQ=255)                                                                          tGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTGGTCGGGGG‑TTTCCCCCCCCGGCGGTATCTGGCACCCCGCCCCATGGGG‑CCCCGGCTTTCCTCCCCTCCCCCCCCCTCCCCCCAAGAGGACGACGACCAAACGGCGA‑c                                                                                                                                                                       >  1:109995/1‑251 (MQ=255)                                                                                     ttGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTCACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCGATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCa                                                                                                                                                             <  1:320549/250‑1 (MQ=255)                                                                                              cTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACTTGGCGTGGGGTTTCCCCCCCGGGGGTT‑TTTTGCACCCCCCCCTATGGGG‑GCCGGGCTTTTCTCCCCCCCGCCCGTCTCCCCCGAAAAGGGAGACGACGAAGGGGCGC‑CTGTCTTGGCCGCTTCGgcgc                                                                                                                                                   >  1:706867/1‑251 (MQ=255)                                                                                              cTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGGCGTGGG‑TTTCCCCCCCCGGCGGTATTTGGCCCCCCGCCCCATGGGG‑CCCCGGCCTTCCTCCCCTCCCCCCCTCTCCCCCCCAGAGGGCGACGACCAAGCGGCGC‑CCGTCTGGTCCGCCTCGgcgc                                                                                                                                                   >  1:250201/1‑251 (MQ=255)                                                                                                ccGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGgcgcg                                                                                                                                                  <  1:87162/250‑1 (MQ=255)                                                                                                   ggTGGAGTTTACCGTGCTACGGACTTTTACCAGCCGCGCGGTGCGATCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACGGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGt                                                                                                                                              <  2:603883/251‑1 (MQ=255)                                                                                                    gTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGta                                                                                                                                             <  1:216821/251‑1 (MQ=255)                                                                                                                        gACTGTTCCCAGCCGCGCTGTGCTCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCGCCCCTCCGCCCGGCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCtt                                                                                                                         <  2:723518/251‑1 (MQ=255)                                                                                                                            gTTACCAGCCGCGCGGTGCGCTCTTCCCGCACCCTTTCGCCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCTGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCGATGGAG‑CCCGGACTTTCCTCCCCGCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCGTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCa                                                                                                                     <  2:513081/251‑1 (MQ=255)                                                                                                                                 cAGCCGCGCGGGGCGCTCTTTCCGCGCCCGTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGGCACCCcg                                                                                                                <  2:605630/251‑1 (MQ=255)                                                                                                                                       cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTgc                                                                                                          <  1:372758/251‑1 (MQ=255)                                                                                                                                             gcgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCAtt                                                                                                     <  1:366316/250‑1 (MQ=255)                                                                                                                                               gcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCCCTGATCGAGGG‑GTTCCCCCCCCGGCGGTTTATTCCCCCCTGCCCTATGGAGGCCCGG‑CTTTTCTCCCCTCCCCCCCGCTCCCCCCCAAAGGGCGACCACGCAGCGGCCG‑CCGTCTGGGCAGCTTCGGCGCGGAGGATAGAGGGGTTGGGCCCCCCTGTCAcccccccgttcgccctccc                                                                                                  >  1:45246/1‑236 (MQ=255)                                                                                                                                               gcTCTTACCGACCCCTGTCACCCTTACCTGATCGCGCTTGCGCGGGCCAGCGGCGGTTTGCTCTCGGTTGCACGGGTCGTGGG‑TTCCCCCCCCAGGCGTTATCTGGAACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCAACCCGCGTTGCGCATTCCa                                                                                                  <  2:193520/251‑1 (MQ=255)                                                                                                                                                      ccGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGTGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCa                                                                                           <  1:696128/251‑1 (MQ=255)                                                                                                                                                                        acccgATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTa                                                                          <  2:228983/246‑1 (MQ=255)                                                                                                                                                                        aCCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTa                                                                          <  1:109209/250‑1 (MQ=255)                                                                                                                                                                          cTGATCCCGCTTGCGCGGGCCATCGGCGGATTGCTCTCTGTTGCACTGGTCGTGGG‑GTTCCCCCCCCGGCGTTATTTGGCCCCCCGCCCCCTGGAG‑CCCGGGCTTTCCTCCCCCCCCCCCCGCCCCCCCCAAGGGGGCGACGACGAAGCGGCGAACT‑TCTGGTCAGCCTCGGCGCGCAGGAAAGAGGGGTTGCGGGCCCCTGTCAACCCCCGTTGCGCATTTCAACCGCCCGTGTCGCGGGGATAtttcgg                                                                       >  1:136172/1‑247 (MQ=255)                                                                                                                                                                              gcccGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGTAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgacgacgac                                                                                                                                                                                   <  1:662861/139‑1 (MQ=255)                                                                                                                                                                               cccGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGc                                                                  <  1:580492/251‑1 (MQ=255)                                                                                                                                                                               cccGCTGGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCcgcg                                                                                                              <  1:650501/207‑1 (MQ=255)                                                                                                                                                                                                    cggTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTTCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGCCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGc                                             >  1:628425/1‑251 (MQ=255)                                                                                                                                                                                                     ggTTTGCTCTCTGTTGAACTGGTCGTGGG‑TTTCCCCCCCAGCCGTTATCTGGCACCCTGCCCTATAGAC‑ACAGGACTTTCCTCCACTCCGCCCCTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTATCTGGTCAGCCTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCCCATTCCAATCGCCAGTGTCGCGGCGCTATTACGTGACCCACGGCAGATATTCTCCTAAGcc                                            >  2:729249/1‑251 (MQ=255)                                                                                                                                                                                                     ggTTTGCTCGCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGcc                                            <  2:109995/251‑1 (MQ=255)                                                                                                                                                                                                      gTTTTCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCTTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCTGTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGccc                                           <  2:105763/251‑1 (MQ=255)                                                                                                                                                                                                        ttGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGTTCAGCTTCGGCTCGCAGTATAGAGTGTTTGCGCGCCCTTGGCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGcccc                                          <  1:692174/250‑1 (MQ=255)                                                                                                                                                                                                          gCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTc                                                                                                                                                        <  1:521615/138‑1 (MQ=255)                                                                                                                                                                                                          gCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTc                                                                                                                                                        >  2:521615/1‑138 (MQ=255)                                                                                                                                                                                                             ctctGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCGCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCgg                                      <  1:77422/249‑1 (MQ=255)                                                                                                                                                                                                               ctGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATg                                  >  1:227654/1‑251 (MQ=255)                                                                                                                                                                                                               ctGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATg                                  >  1:683378/1‑251 (MQ=255)                                                                                                                                                                                                               ctGTTGCACTGGTCGTGGG‑TTGCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGaa                                    <  1:425369/249‑1 (MQ=255)                                                                                                                                                                                                                 gTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATCTTACGTGCAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCt                                >  2:329031/1‑251 (MQ=255)                                                                                                                                                                                                                 gTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGAGATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCt                                <  1:74398/251‑1 (MQ=255)                                                                                                                                                                                                                          gTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCCCCCGGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAAc                       <  2:733959/251‑1 (MQ=255)                                                                                                                                                                                                                            cGTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGt                     <  2:250201/251‑1 (MQ=255)                                                                                                                                                                                                                             gTGGG‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGCAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGGGAAGCACGGAAGTTATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGtc                    >  2:471606/1‑250 (MQ=255)                                                                                                                                                                                                                              tGGG‑TTTCCCCCACAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCAGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGCCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGAGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAGGCACGGCAGATATTGTCATAAGCCCCGCGGAATGATTCGTCCAACGTAc                   >  1:452471/1‑251 (MQ=255)                                                                                                                                                                                                                                gg‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGCCGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCACCGTAccc                 >  2:469611/1‑250 (MQ=255)                                                                                                                                                                                                                                gg‑TTTCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCt                 >  1:356927/1‑251 (MQ=255)                                                                                                                                                                                                                                     tCCCCCCCAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGc             >  1:649751/1‑251 (MQ=255)                                                                                                                                                                                                                                      cccccccAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCt            <  1:86594/251‑1 (MQ=255)                                                                                                                                                                                                                                           ccAGGCGTTATCTGGCACCCTTCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCCCCCAACGTACCTATGCTGGTc        >  2:458633/1‑250 (MQ=255)                                                                                                                                                                                                                                           ccAGGCGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCa       <  1:293537/251‑1 (MQ=255)                                                                                                                                                                                                                                           ccAGGCGTTATCTGACACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTATCCCCCGAAGCGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATCGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTc        >  2:103364/1‑250 (MQ=255)                                                                                                                                                                                                                                                cGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCAATACg  >  1:174145/1‑251 (MQ=255)                                                                                                                                                                                                                                                cGTTATCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCAATACg  <  1:482293/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        AGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAG‑CCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGA‑CTGTCTGGTCAGCTTCGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCAATACG  >  NC_000913/3270254‑3270742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑