| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,224,895 | C→T | L755L (CTG→TTG) | ebgA → | evolved beta‑D‑galactosidase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,224,895 | 0 | C | T | 98.4% | 418.6 / NA | 125 | L755L (CTG→TTG) | ebgA | evolved beta‑D‑galactosidase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (64/57); minor base G (1/1); total (67/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
TCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGG > NC_000913/3224650‑3225141 | tCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACt > 1:76179/1‑251 (MQ=255) tACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGc > 1:609205/1‑251 (MQ=255) ggTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACCCCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCaa < 2:16647/251‑1 (MQ=255) ggTGACCAAAGATGCCCGCCCCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCGCGCTGAAGGAAAACACCGCTCAGCCAGTGCCTTTCGCACCAAAGAATGCGCTGCCGCGGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACGTCGCGAGCACCTTCTCAAAAATGAGTGGCAAGCCGACAGCCTGGCAGGGGAATGGCGAATCG‑TGGTCGACTCGCGAGCCaa < 2:709042/251‑1 (MQ=255) gTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCaaa < 1:643544/251‑1 (MQ=255) ccAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATc < 2:605124/251‑1 (MQ=255) ccAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCGTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATc < 1:89843/251‑1 (MQ=255) aaaGATTCCCGCACCCGATACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGCGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCac > 2:486560/1‑250 (MQ=255) aggttCCCTCACCCGCGACAGCGAAGCCGGACACACGACCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTATCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACAGTTCGCGGCTACATCTTCGCGATCATCTTCTCAAAAATGAGTGGCAAACCGACAACCTTGCAGGTGAATGGCGAATCTCTGTT‑GACTCGCGCGCCAAAGATCAAct < 2:200067/248‑1 (MQ=255) ccGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTTCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACAGCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCa < 2:353731/251‑1 (MQ=255) cgcccccGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCaa < 2:314416/247‑1 (MQ=255) cACCCGCTACAGCGAAGCCGGACTCCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCGGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGc < 2:686144/251‑1 (MQ=255) aCCCGCTACAGCGAAGCCGGACACCCTATCGCAACTTATCAGTTCCCGCTGCAGCAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGAGCGTCCGCTGACCCTGGAAGACGATCGTTTGAGCTACCCCGCTCGCGACTACAACTTCGCGATCACCTTCTCAAAAATGAGAGGCAAACCGACATCCTGGCAGGTGAATGGCGCATCGCTGAT‑AACTCCCGAGCCAAAGATCCACTTCTTCAAGcc > 1:451065/1‑251 (MQ=255) ccGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCgc > 1:259715/1‑250 (MQ=255) cGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTTCAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCgat > 2:246481/1‑251 (MQ=255) gCTACAGCGAAGCCGGACACCCTATCGCCCCTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCTCTGACGTTGGAAGACGATCTTTTGAGCGGCACCGTTCGCGGCTACATCTTCTCGATCACCGTCTCAAAAAGGAGTGGCAAACCGACATCCTGGCAGTTGAATGGCGAATCGCTGTT‑GATTCGGCAGCCAAAGTTCAACTTCTTGAAGGCgatg < 2:341983/251‑1 (MQ=255) gCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCgctg > 1:636294/1‑248 (MQ=255) aCAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGTCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GCCTAGCGAACCAACGATCAACTTCTTCCAGCCGATGATc > 2:388941/1‑251 (MQ=255) gAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GAATCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACa > 2:63135/1‑250 (MQ=255) gCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAATCACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACca < 1:414751/251‑1 (MQ=255) ggACACCCTATCGCCACTTCTCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GAATCGCGAGCCAAAGCTCAACTTCTTCAAGCCGATAATCGACAACcaca > 1:627349/1‑250 (MQ=255) ggACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCGAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACcaca > 2:205746/1‑250 (MQ=255) cacCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATTAGTGGCAAACCGACATCCTGGCAGGTGAATGTCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCa < 1:312614/251‑1 (MQ=255) ccTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAgg > 1:19643/1‑250 (MQ=255) cGCCACTTATCAGTTCCCGCTGAAGGAAAACCCCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCGTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACg < 2:547149/251‑1 (MQ=255) cACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGa > 2:14826/1‑249 (MQ=255) ttATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGc > 2:648554/1‑251 (MQ=255) tATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGc < 1:722644/250‑1 (MQ=255) aTCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCt < 1:235281/250‑1 (MQ=255) tCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCa < 1:192748/250‑1 (MQ=255) ccGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAAcc < 1:373753/251‑1 (MQ=255) ccGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAAcc < 1:147218/251‑1 (MQ=255) ggAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCAtt > 1:272143/1‑251 (MQ=255) ggAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCAtt < 1:22286/251‑1 (MQ=255) gAAAACACCGCGCAGCCAGTGCCTTTCGCCCCAAATCATGCGCGTCCGCTGACGCTGGAAGACGATCTTTGGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAATCCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCTACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCAttt < 2:672298/251‑1 (MQ=255) aaaaCCCCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTg < 2:35718/251‑1 (MQ=255) aaaaCACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATTAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGCGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTg > 1:35406/1‑251 (MQ=255) acacCGCGCATCCAGTGCCTTTCGCACCAACTAATGCGCGTCCGATGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGAGGCTCCAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGCATGGCGAATCGCTGTT‑GACTCGCGCGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGAGGCAACCGAATCATTTga > 2:465770/1‑248 (MQ=255) cacCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGc > 1:499032/1‑248 (MQ=255) ccGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTTCAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATc < 1:246481/251‑1 (MQ=255) cgcAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGCATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATg > 2:81554/1‑251 (MQ=255) gcAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGc < 1:648554/251‑1 (MQ=255) cAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGc > 1:660092/1‑250 (MQ=255) ccAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGCTGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAgg > 1:210900/1‑250 (MQ=255) gTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAAc > 1:552540/1‑250 (MQ=255) tGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACAt > 2:607734/1‑251 (MQ=255) cGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTgcgc < 2:230571/250‑1 (MQ=255) gCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACCACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGcgcg > 2:111388/1‑250 (MQ=255) aCCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTAGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACt > 1:438694/1‑251 (MQ=255) aataatGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAAACGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGc > 2:532706/1‑251 (MQ=255) aatGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACAGCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCg < 2:76179/250‑1 (MQ=255) aatGCGCGTCCGCTGACGCTGGAAGACGAGCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTACAGATCATGCAGGAACATCTGCGCGACTTTGCCgg > 2:173408/1‑250 (MQ=255) gcgTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAAc > 1:352053/1‑251 (MQ=255) cgTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAACTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCCTTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAAc > 2:486398/1‑250 (MQ=255) ggcccctcaCGCTGGAAGACGATCGTTGGAGCTGCACCGCTCGCGGCTACAACTTCGCGATCCCCCTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACag < 2:397407/243‑1 (MQ=255) gTCCGATGAAGCTGGAAGACGATCGTTTGCGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACCTCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGCATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAAc > 2:42898/1‑249 (MQ=255) tGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGa > 1:141159/1‑250 (MQ=255) gggAAGACGATCGTTTGAGCTGCACCGTGCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGaa < 2:636294/250‑1 (MQ=255) ggAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGaa < 1:542323/250‑1 (MQ=255) gAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGAt < 2:236581/243‑1 (MQ=255) gAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGAt > 1:236581/1‑243 (MQ=255) actaTCGTTTGAGCTGCACCGTCCGCTGCTACAACGTCGCGAGCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GATTCGCGAGGCAAAGATCAACGTCTGCAAGCCGATTATCGACAACCACAAGCAGG‑TGTTTGAAGGGCTGTGGCAACCTAATCATTTGCAGATCATGCAGGAACATCGGCGCGACTTTGCCGTAGAACGGAGCGATGGTGAATTGCTg < 2:737533/248‑1 (MQ=255) gATCGTTTGAGCTGCACAGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGACTGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGat > 2:488742/1‑251 (MQ=255) aTCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCGGTT‑GACTCGCGAGCCAAAGATCAACTGCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGatc < 1:81554/251‑1 (MQ=255) cGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGatcat < 1:532706/251‑1 (MQ=255) cGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGatca < 2:552540/250‑1 (MQ=255) tttGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAACGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGCGAAGTGCTGatcatc > 2:403444/1‑250 (MQ=255) ttGAGCTGCACCGTGCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCGGGCAGGTGAATGGCGAATCGCTGTG‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGAGGAGCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTGGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCTATGGTGAAGTGCTGAtcatca < 2:60422/250‑1 (MQ=255) gaaacTGCAACGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACAACCGGGCAGGTGAATGGCGAATCGCAGTT‑GACTCGCGAGCCAAAGAACAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATTATGCAGGAACATCTGCGCTACTGTGCCGTAGAACAGAGCGATGGTGAAGTGCTGAtcatcatc < 2:523830/247‑3 (MQ=255) tGCACCGTTCGCGGCTACAACTTCGCGATCCCCTTCTCACAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGccgg > 2:472254/1‑142 (MQ=255) tGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTAAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAgg < 1:472254/145‑1 (MQ=255) gCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGcac < 1:22884/250‑1 (MQ=255) cACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGACTGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGcac > 1:673342/1‑249 (MQ=255) cACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGcac > 1:95700/1‑249 (MQ=255) ttCGCGTCTACAACTTCGCGTTCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATTGCGCATCGCTGTT‑GACTCGCGAGCCAAAGATCCACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACCGTTAtt > 2:145180/1‑251 (MQ=255) gcgGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGCAGTGCTGATCATCCGCCGCACAGTTATTGcc > 2:479389/1‑251 (MQ=255) cgGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCGGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGccc < 2:663190/251‑1 (MQ=255) cTACAACTTCGCGATCACCTTCTCAAAACTGAGTGGCAAACCGACATCATGGCAGGAGAATGGCGAATCGCTGTT‑GACTCGCGAGCAAAAGATAAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCCTACAGGAACATCTGCGCGCCTTTGCCTTAGCACAGAGCGATGGTGAAGGGCTGATCATCCCCCTCACAGTTATTCCCccgc > 2:263966/1‑251 (MQ=255) tCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGACCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGtt < 1:63135/250‑1 (MQ=255) gcgATAACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGGGTTTg > 2:731875/1‑250 (MQ=255) gATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTgcct > 1:266812/1‑248 (MQ=255) gATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGAT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATATGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACt > 1:356027/1‑251 (MQ=255) aTCACCTTCTAAAAAATGAGGGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTG‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACtt < 1:235676/251‑1 (MQ=255) cccAAAAATGAGTGGCAAACCGCCATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCAAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATg < 1:123503/248‑1 (MQ=255) aaaaaTGAGTGGCAAACCGACATCCTGGCGGGTGAAGGGCGAATCGCGGTT‑GACTCGCGAGCAAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATGGCCCCGCCGGTGTTTGACTTCGGGATGCGCt < 2:493525/251‑1 (MQ=255) ggCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACAt > 1:297206/1‑251 (MQ=255) cccgccctCCTGGCGGGGGAAGGGCAAACCGCTGTT‑GCCTCGCGAGCAAAGGCTCACCTTCTCCAAGCCGATGAACGACAACCACAA‑CAGGGAGTACGAAGGGCGGTGGCAACCGAATCTTTGGCAGATCAGGCAGGAACATCTGCGCGTCTTTGCCGTAGAACAGAGCGAGGGTGAAGTGCTGATCATCAGCCGCACGGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGgc < 2:338030/244‑1 (MQ=255) aCCGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTCCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGgc > 1:60145/1‑251 (MQ=255) cGACATCCTGGCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGgcgc > 1:135473/1‑251 (MQ=255) cATCCGGGCAGGGGAAGGGCGAATCGCTGTT‑GACCCGCGAGCAAAAGATCAACTTCTCAAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCACCCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAATGGCTGATCACCAGCGGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATc < 2:210900/251‑1 (MQ=255) gCAGGTGAATGGCGCATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGAAGG‑AGAACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGCTGGTGAAGTGCTGATCATCAGCCACACAGTTATTGCCCCGCCGGTGTTTGACTTAGGGATGAGCTGCACCTACATCTGGCGCATCGCTACCg > 1:468161/1‑251 (MQ=255) gCAGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTc > 1:313867/1‑214 (MQ=255) gCAGGGGAATGGCGAATCGCTGTT‑GACCCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGGGAAGTGCTGATCATCAGCCGCACGGTTATTGCCCCGCCGGTGTTAGACTTc < 2:313867/214‑1 (MQ=255) aGGTGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGAt > 2:207294/1‑251 (MQ=255) tGAATGGCGAATCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGc > 2:358985/1‑251 (MQ=255) cgactCGCTGGT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTCCGACGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGCACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATGGCCCCGCCGGTGTTTGACTTCGGAATGCGCTGGACCTACATCTGGCGCGTCGCTGCCGATGGCCAGGtt > 2:450191/5‑250 (MQ=255) aaTCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAgg > 1:295525/1‑246 (MQ=255) tCGCTGTT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGt > 1:357620/1‑251 (MQ=255) cGCTGTT‑GACCCGCGGGCCAAAGATCAACTTCTCCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGAAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGTGGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTg < 2:609205/251‑1 (MQ=255) gctgtt‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATGGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAAACATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATAATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGTATGCGCTGCACCTACATCTTGCGCATCGCTGCCGCTGGCAAAGATAACGTgg > 1:432912/1‑251 (MQ=255) | TCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCT‑GACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGG‑AGTACGAAGGGCTGTGGCAACCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGG > NC_000913/3224650‑3225141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |