Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,204,617	G→A	intergenic (‑28/‑77)	folB ← / → plsY	dihydroneopterin aldolase/putative glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,204,617	0	G	A	100.0%	248.8 / NA	71	intergenic (‑28/‑77)	folB/plsY	dihydroneopterin aldolase/putative glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/39);  total (32/39)

TTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCC  >  NC_000913/3204371‑3204858                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  ttCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGAt                                                                                                                                                                                                                                                >  2:174431/1‑250 (MQ=255)  tcccccaGCGCAAACCGCGCCCCCTCGCCGTGGCTGCCCACCGTTTCTGCAATGTCAGCGTAACTGAGCCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATa                                                                                                                                                                                                                                               <  2:591054/246‑1 (MQ=255)    caccaGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATAcc                                                                                                                                                                                                                                             >  2:224545/1‑250 (MQ=255)      ccaGCGCAAAACGCGCCCCCTCGCCGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACt                                                                                                                                                                                                                                          <  2:269602/251‑1 (MQ=255)                        ccTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTT‑GGCTAACCAGATACCACTTCCGGCGAAATGTGCGtat                                                                                                                                                                                                                       <  1:453754/251‑1 (MQ=255)                          tCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTCACCAGATACCACTTCCGGCGAAATGTGCGtatt                                                                                                                                                                                                                      >  2:24520/1‑251 (MQ=255)                           cGACGTGGCTCCCCCCCGTTTCTGCAAGGCCAGCGTAACTGGGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATTTGCGtatta                                                                                                                                                                                                                     <  1:399257/251‑1 (MQ=255)                            gACGTGGCTAACCTCCGTTTCTGCATTGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGtattat                                                                                                                                                                                                                    <  1:685145/251‑1 (MQ=255)                                     gACCACCGTTTCGGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGAtt                                                                                                                                                                                                           <  1:253907/251‑1 (MQ=255)                                      acccccGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTc                                                                                                                                                                                                          <  1:500803/247‑1 (MQ=255)                                             tttCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACT‑CCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTg                                                                                                                                                                                                   >  1:239243/1‑250 (MQ=255)                                                  gCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGaa                                                                                                                                                                                                 <  1:581580/248‑1 (MQ=255)                                                  gCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAac                                                                                                                                                                                                <  1:99464/249‑1 (MQ=255)                                                   cAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACg                                                                                                                                                                                             <  1:199867/251‑1 (MQ=255)                                                     aTGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGaa                                                                                                                                                                                           <  1:580707/251‑1 (MQ=255)                                                      tGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAAt                                                                                                                                                                                          >  1:267305/1‑251 (MQ=255)                                                       gTCAGCGGAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCGAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAAtt                                                                                                                                                                                         <  2:431904/251‑1 (MQ=255)                                                                   gAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGa                                                                                                                                                                              >  2:272298/1‑250 (MQ=255)                                                                      gCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAAc                                                                                                                                                                            >  1:198782/1‑249 (MQ=255)                                                                          cccGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTGCAAAGCGGAACAGCTTa                                                                                                                                                                      <  2:380348/250‑1 (MQ=255)                                                                              ccACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATGACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGAGTCATCGTTGAACACGAATTGTCAAAACGGAACAGCATATGAg                                                                                                                                                                  <  2:328669/251‑1 (MQ=255)                                                                                  atcatcACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCGTATGAGTGCa                                                                                                                                                              <  2:111790/251‑1 (MQ=255)                                                                                                 cgcTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATg                                                                                                                                               >  2:662449/1‑251 (MQ=255)                                                                                                   cTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTCCCAAAGGCTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGAGTATTATCCACAGATTCATCGTTAAAAACGAATTTTCAACACGGAACAGTTTATGAGTGCAATCGCGCCTGGATTGAt                                                                                                                                             >  1:435461/1‑251 (MQ=255)                                                                                                     ttACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACTTCCTGCCTGCTTTTTGGCTAACCAGATACCACGTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATc                                                                                                                                            <  2:18256/250‑1 (MQ=255)                                                                                                        cGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTc                                                                                                                                         >  1:488783/1‑250 (MQ=255)                                                                                                         ggTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCAt                                                                                                                                       <  2:95218/251‑1 (MQ=255)                                                                                                          gTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATc                                                                                                                                      >  2:511844/1‑251 (MQ=255)                                                                                                          gTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATc                                                                                                                                      >  1:693285/1‑251 (MQ=255)                                                                                                           ttATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACCACAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATcg                                                                                                                                     >  1:658126/1‑251 (MQ=255)                                                                                                                ccACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTAc                                                                                                                                <  2:16433/251‑1 (MQ=255)                                                                                                                 cACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTAcc                                                                                                                               <  2:198782/251‑1 (MQ=255)                                                                                                                 cACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTAcc                                                                                                                               <  1:389788/251‑1 (MQ=255)                                                                                                                       aTTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCg                                                                                                                         >  2:149527/1‑251 (MQ=255)                                                                                                                         ttCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGc                                                                                                                       >  1:674346/1‑251 (MQ=255)                                                                                                                           cGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCt                                                                                                                      <  2:709267/250‑1 (MQ=255)                                                                                                                            gATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACCATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTcc                                                                                                                    >  2:292894/1‑251 (MQ=255)                                                                                                                            gATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTcc                                                                                                                    >  2:225384/1‑251 (MQ=255)                                                                                                                                 cGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCAttt                                                                                                                <  1:224545/250‑1 (MQ=255)                                                                                                                                 cGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTc                                                                                                               <  2:72869/251‑1 (MQ=255)                                                                                                                                 cGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTGCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCAttt                                                                                                                <  2:569138/250‑1 (MQ=255)                                                                                                                                                      tGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGAAATCGCGCATGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCg                                                                                          >  2:587930/1‑251 (MQ=255)                                                                                                                                                               cccAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTgg                                                                                 <  1:501963/251‑1 (MQ=255)                                                                                                                                                                  aGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGGGTGGGCt                                                                              <  2:488783/251‑1 (MQ=255)                                                                                                                                                                         aaCACCAATATTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCGTTTTGGCGAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGTAACAGCTTATGAGTGCAAACTCGCCTGGAATGATCCTCAGCGCGTACCTCTGCGGCTCCATTGCCAGTGCCATTTTTGTTTGCCGCGTGTTTGGGCTGGCCGAt                                                                       <  2:425132/251‑1 (MQ=255)                                                                                                                                                                          acacCAAGAGTGGTGATTACCGAAAGTTGCTCTAGAAATACAATATCCATCACGTCCTGCCTGCTTTGTGGCTAACCAGATACCACTTCCGGCGGAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCTTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATGCTGGTTTGCCGCTTGTGTGGGCTGCCCGATc                                                                      <  2:680109/251‑1 (MQ=255)                                                                                                                                                                          acacCAAGAGTGGGGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTGCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCTGCTCCATTTCCAGTTCCATGCTGGTTTGCGGCTTGGGTGGGCTGCGCGATc                                                                      <  2:444980/251‑1 (MQ=255)                                                                                                                                                                           cacCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATcc                                                                     >  2:383523/1‑251 (MQ=255)                                                                                                                                                                             ccAATAGGGGTGATTACCGAAATTTGTACTATAAATACAATAGCCATCACGTCCTGCCTGCTTTTTGGATAACCAGATTCCACTGCCGGCGAAATGTGCGTATTATCCTCAGATTCATAGTTGAACACGAATTTTCAAAACGGAACAGCTTAAGAGGGCAATAGCGCCTGGAATGATCCTCAGCGCGTACCTGTGCGGCTCCATTTCCAGGGCCAGGCGGGTTGGCCGCTTGTGTGGGCTGCACGATCcgc                                                                   <  2:90972/251‑1 (MQ=255)                                                                                                                                                                                           tACCGAAAGTTGCTCTATCAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTcc                                                     >  1:559304/1‑251 (MQ=255)                                                                                                                                                                                             ccGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCgg                                                   <  1:77291/251‑1 (MQ=255)                                                                                                                                                                                               gAAAGTTGCTCTATAAATACAATAGCCATCACGTCCTGACTGCTTTTTGGCTAAACAGAGACCACTTCCGGCGAAATGTGCGTATTATCCAAAGATGCATCGTGGAACACGAATTTTCAAAACGGAACAGCTTATGAGGGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCCCCATTTCCAGGGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGAGCCGCGAACCAGCGGCTCCGGCa                                                 <  2:638112/251‑1 (MQ=255)                                                                                                                                                                                                   gTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATcc                                             >  2:160674/1‑251 (MQ=255)                                                                                                                                                                                                   gTTGCTCTATAAATACAACATCCATCACGTCCTGCCTGCTTTTTGTCTAACCAGATACCCCTTCCGGCGAAATTTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATcc                                             <  1:49953/251‑1 (MQ=255)                                                                                                                                                                                                   gTTGCTCTATAAAGACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCTGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATcc                                             <  1:94724/251‑1 (MQ=255)                                                                                                                                                                                                    ttGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCACTccc                                            >  1:334221/1‑250 (MQ=255)                                                                                                                                                                                                      gCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAgg                                          >  1:297099/1‑251 (MQ=255)                                                                                                                                                                                                       ctctATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGgc                                         >  1:124279/1‑251 (MQ=255)                                                                                                                                                                                                                   aaTATCCATCACGTACTGCCTGCTTTTTGGCTAACCAGTTACCCCCTCCGGCGAAATGTGCGTCTTATCCACAGGTTCATCGTTGAACACGCATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGATTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCTGGCGCAACCAAtgcg                             >  2:319227/1‑249 (MQ=255)                                                                                                                                                                                                                    atatCCATCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATgtgt                            >  2:154977/1‑251 (MQ=255)                                                                                                                                                                                                                          aTCACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGt                       >  1:630523/1‑250 (MQ=255)                                                                                                                                                                                                                            cACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATc                    >  2:260589/1‑251 (MQ=255)                                                                                                                                                                                                                            cACGTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATc                    >  1:659901/1‑251 (MQ=255)                                                                                                                                                                                                                               gTCCTGCCTGCTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGt                 <  1:225384/251‑1 (MQ=255)                                                                                                                                                                                                                                   tgcctgcTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGc              >  1:689527/1‑250 (MQ=255)                                                                                                                                                                                                                                    gcctgcTTTTTGGCTAACCAGATACCCCTTCCGGCGAGATGTGCGTATTATCCCCCGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGCACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGCGTTACGTATCGGTGGCa             >  2:486330/1‑250 (MQ=255)                                                                                                                                                                                                                                        gcTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAggg         <  2:405731/250‑1 (MQ=255)                                                                                                                                                                                                                                        gcTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGa        <  1:265651/251‑1 (MQ=255)                                                                                                                                                                                                                                        gcTTTTTGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGa        <  1:392890/251‑1 (MQ=255)                                                                                                                                                                                                                                         cgttttGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTAGCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGat       <  2:621053/249‑2 (MQ=255)                                                                                                                                                                                                                                              tGGCTAACCAGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGcc  >  1:399380/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  TTCCACCAGCGCAAAACGCGCCCCCTCGACGTGGCTGACCACCGTTTCTGCAATGTCAGCGTAACTGAGGCAATCCGCCACATCATCACTTTTCGCCGCTTTACGGTTATCCCACGCCATTTCGATATCGAACACTAACTTCTGTTCGATGGTCTGTTCCCAGTCGTAAACACCAATAGTGGTGATTACCGAAAGTTGCTCTATAAATACAATATCCATCACGTCCTGCCTGCTTTTTGGCTAACCGGATACCACTTCCGGCGAAATGTGCGTATTATCCACAGATTCATCGTTGAACACGAATTTTCAAAACGGAACAGCTTATGAGTGCAATCGCGCCTGGAATGATCCTCATCGCGTACCTCTGCGGCTCCATTTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCC  >  NC_000913/3204371‑3204858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑