Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,176,625	G→A	S70F (TCC→TTC)	cpdA ←	cAMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,176,625	0	G	A	97.9%	336.1 / NA	95	S70F (TCC→TTC)	cpdA	cAMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (34/59);  minor base C (2/0);  total (36/59)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGG  >  NC_000913/3176378‑3176869                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      ggCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAAtt                                                                                                                                                                                                                                                    <  1:625512/251‑1 (MQ=255)   ccAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCGCACCGTGCGGCACGCCAAACACCGGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGa                                                                                                                                                                                                                                                  <  2:229023/251‑1 (MQ=255)            gTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCGTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCt                                                                                                                                                                                                                                         <  1:301916/251‑1 (MQ=255)             tcccAGCCACTCAATCTGAAACTCTCTCAGCTCCCCTTTCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATTCCCGCATCCTGTAACGCGCTGTACATCGCTGGCTCGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCt                                                                                                                                                                                                                                         <  1:118991/248‑1 (MQ=255)              tCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTa                                                                                                                                                                                                                                        >  1:28232/1‑250 (MQ=255)               ccAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTaaa                                                                                                                                                                                                                                      <  1:716041/251‑1 (MQ=255)                cAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGTCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGTGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAATTGCTGATAGGCCGCATAGAATTGATCCTGCGCTAAAt                                                                                                                                                                                                                                     <  2:141067/251‑1 (MQ=255)                   ccACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCAc                                                                                                                                                                                                                                  <  1:655395/251‑1 (MQ=255)                       ccAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAAGCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGt                                                                                                                                                                                                                              <  2:483872/250‑1 (MQ=255)                           gCTGAAACTCGCTCAGCGCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTcgcg                                                                                                                                                                                                                          <  2:440617/251‑1 (MQ=255)                                     gctcagctcaCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGt                                                                                                                                                                                                                >  2:50722/1‑251 (MQ=255)                                             caCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAAtt                                                                                                                                                                                                          <  2:523926/249‑1 (MQ=255)                                             caCCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCg                                                                                                                                                                                                        <  2:28232/251‑1 (MQ=255)                                                 gTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAAGGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCt                                                                                                                                                                                                    <  2:327352/251‑1 (MQ=255)                                                     ggctcGCCAAACACCTGGCTATCTAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTTTACATCGCGGGCTGGAAATCGTTGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTTATAGGCCGCAGAGAATTGATCCTGCGCTAAATCGCCTGTCGCGACAATCAGGTCGAATTCGTGCTGgtg                                                                                                                                                                                                <  1:444763/247‑1 (MQ=255)                                                     ggCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCTCCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCTCGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAAGTGATCCTGCGCTAAATCACCTGTCGCGTCAATCAGGTCGAATTCGTGCTGgtg                                                                                                                                                                                                <  1:420878/251‑1 (MQ=255)                                                       cACGCCAAACACCTGTCTATCCATCCACAGGATTGGCCATTGCTCATCAATAAACTCGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGTGCTTGAAATCGTGGTTGACCGGCAGCCAGACGCAGGGTGCACGAAAACTTGCGATTCCTGCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCGGTCGCGACAATCAGGTCGAATTCGTGCTGgtgtt                                                                                                                                                                                              <  2:627414/251‑2 (MQ=255)                                                         cGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCAGTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCTCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATGCGTGTTGTTGTGGc                                                                                                                                                                                            <  2:677453/251‑1 (MQ=255)                                                              aaCACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCAACAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAAt                                                                                                                                                                                       <  2:556782/251‑1 (MQ=255)                                                               acacCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATc                                                                                                                                                                                      <  1:129512/251‑1 (MQ=255)                                                                cacCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCg                                                                                                                                                                                     >  1:278913/1‑251 (MQ=255)                                                                 acCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGTGACAATCAGGTCGAATTCGTGCTGGGGTGGCCGAATCGc                                                                                                                                                                                    >  2:442181/1‑251 (MQ=255)                                                                     ggCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCt                                                                                                                                                                                  <  2:488913/249‑1 (MQ=255)                                                                         aTCCAGCAACAGGATTTGCCCTTGATCACCAATAAACACGCGCTGCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTCCATCGCGGGCTGGAAATCGGGGTTGTCCGGCAGTCAGTCGCAGGGCGCACGAAAACTTGCGATGCCGTCAGCGATATGCTGATAGGACGCAGAGAGTTGATCCTGCGCTAAATCATCTGTCGCGACAATCAGGACGAATTCGTGCTTGTGTGGCCTAATCGCCTCCAg                                                                                                                                                                              <  2:684914/249‑1 (MQ=255)                                                                         aTCCAGCAACAGGATTTGCCAGTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGc                                                                                                                                                                             <  1:716227/250‑1 (MQ=255)                                                                           ccAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCAcc                                                                                                                                                                          <  1:439818/251‑1 (MQ=255)                                                                           ccAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCAcc                                                                                                                                                                          <  1:406062/251‑1 (MQ=255)                                                                               cAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCt                                                                                                                                                                      <  1:613014/251‑1 (MQ=255)                                                                                  cAGGCTTTGCAATTGCTAACCAATATACACGCGCTTAGCCGGGGAGCTACCCGAATCCTGTCGCGCGCTGTACATCGAGGGCTGGACATCGTGGTTGCCCGGCAGCCAGCCGCACGGCGCACGAAAACTTGCGATGCCTTCAGCGACATGATGATAGGCCGCCGAGCATTGATCCTGCTCTCAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTggc                                                                                                                                                                   >  2:348846/1‑250 (MQ=255)                                                                                  cAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGt                                                                                                                                                                   <  2:214039/251‑1 (MQ=255)                                                                                      aTTTTCCATTGCTCACCAATAAGCACTCGCTTCGCCGGGGAGATTCCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCTGGGCGCACGAAAACTTGCTATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTTGTAAct                                                                                                                                                               <  1:519740/251‑1 (MQ=255)                                                                                          gCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTcc                                                                                                                                                           <  2:394453/251‑1 (MQ=255)                                                                                           ccATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCCGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGCATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTccc                                                                                                                                                          >  1:335668/1‑251 (MQ=255)                                                                                           ccATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATCCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTAATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTccc                                                                                                                                                          >  1:415731/1‑251 (MQ=255)                                                                                            cATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTcccc                                                                                                                                                         >  2:210276/1‑250 (MQ=255)                                                                                            cATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCa                                                                                                                                                         <  1:480701/251‑1 (MQ=255)                                                                                             aTTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGGCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCGCCCAg                                                                                                                                                        <  2:504951/251‑1 (MQ=255)                                                                                              ttGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAgg                                                                                                                                                       >  1:579546/1‑251 (MQ=255)                                                                                                 cTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGGGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGCCGAATTCGTGCTGGGGGGGCCGAATCGCCTCCAGAACCGCCTGGTAACTCTCCCAGgtgt                                                                                                                                                    >  2:526016/1‑251 (MQ=255)                                                                                                   cACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGgtgt                                                                                                                                                    <  1:719002/249‑1 (MQ=255)                                                                                                      cAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTAcc                                                                                                                                               >  2:522894/1‑251 (MQ=255)                                                                                                      cAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTAcc                                                                                                                                               <  2:331001/251‑1 (MQ=255)                                                                                                       aaTAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAgg                                                                                                                                                       >  1:254272/1‑242 (MQ=255)                                                                                                       aaTAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAgg                                                                                                                                                       <  2:254272/242‑1 (MQ=255)                                                                                                        aTAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACAGCGCGGGCTGGAAATCGTGGTGGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCGGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAACCGCCTCCAGCCCCGCCTGGTAACTCTCCCAGGTGTTTAcccc                                                                                                                                             <  2:446992/251‑1 (MQ=255)                                                                                                         tAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAACTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCt                                                                                                                                            >  2:645015/1‑251 (MQ=255)                                                                                                          aaaCACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCt                                                                                                                                            <  1:577965/250‑1 (MQ=255)                                                                                                            acacGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAAc                                                                                                                                         >  1:393710/1‑251 (MQ=255)                                                                                                            acacGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAAc                                                                                                                                         <  2:579546/251‑1 (MQ=255)                                                                                                                  gcTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCt                                                                                                                                   >  1:571460/1‑251 (MQ=255)                                                                                                                       gCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTg                                                                                                                              <  2:180360/251‑1 (MQ=255)                                                                                                                       gCCGGGGAGATACCCGCATCCTGAAACGCGCTGTACCTCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGAGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAACTCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGATTCGTg                                                                                                                              >  2:466996/1‑251 (MQ=255)                                                                                                                             gagaTACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTtg                                                                                                                        >  2:589571/1‑251 (MQ=255)                                                                                                                              agaTACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTtgt                                                                                                                       >  1:575277/1‑251 (MQ=255)                                                                                                                                     cGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAAc                                                                                                                 >  2:259983/1‑250 (MQ=255)                                                                                                                                       cATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGTCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAgg                                                                                                              <  1:63199/251‑1 (MQ=255)                                                                                                                                        aTCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAggg                                                                                                             >  2:481094/1‑250 (MQ=255)                                                                                                                                             gTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAgtg                                                                                                       >  2:505895/1‑251 (MQ=255)                                                                                                                                               aaCGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAgtgt                                                                                                      <  1:44929/250‑1 (MQ=255)                                                                                                                                               aaCGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTc                                                                                                     <  2:25133/251‑1 (MQ=255)                                                                                                                                                 cgcgCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCgg                                                                                                   <  1:24709/251‑1 (MQ=255)                                                                                                                                                   cgcTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGt                                                                                                  >  2:221123/1‑250 (MQ=255)                                                                                                                                                                gggCGGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATATGCCGCAGAGAATTAATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTACAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATcc                                                                                    <  2:415731/251‑1 (MQ=255)                                                                                                                                                                         aTCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTgg                                                                           <  1:248236/251‑1 (MQ=255)                                                                                                                                                                            ggggTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTGGCGATGCCTTCAGCGAAATGCTGATATGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAAGCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCt                                                                        <  2:608334/249‑1 (MQ=255)                                                                                                                                                                              ggTTGCCCTGCAGCCATACGCAGGGCGCACGAAATCTTTCTCTGCCTTCCGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCTCTAAATCTCCTGTCCCGACAAGCAGGTCGATTTCGTTCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCTTGTGTTTACCCCTAACATGTCTTCGTGCTTTGTTGCAAACAGG‑TGAGTATCGGTAATTTGTAAAATCCTGCCTCTGGCCTc                                                                       <  1:419954/250‑1 (MQ=255)                                                                                                                                                                              ggTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCa                                                                      <  2:23716/251‑1 (MQ=255)                                                                                                                                                                                 tGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccc                                                                   >  2:243517/1‑250 (MQ=255)                                                                                                                                                                                     cGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagcca                                                               <  2:571460/251‑1 (MQ=255)                                                                                                                                                                                      ggCAGCCAGACGCAGGGCGAACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCAccagcc                                                                >  1:207578/1‑249 (MQ=255)                                                                                                                                                                                        cAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCagag                                                            <  1:500438/251‑1 (MQ=255)                                                                                                                                                                                         aGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAgg                                                           <  2:307644/251‑1 (MQ=255)                                                                                                                                                                                            caaaCGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGa                                                          >  1:277222/4‑249 (MQ=255)                                                                                                                                                                                                cgcatagcgcACGAAACCTTGCGATGCCTACGGCGCACTGCTGATAGACCGCAGAGAATAGCTCCTGCGCTAAATCAACTCTCGCGACAATCCGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGACACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGtt                                                    >  2:715604/7‑251 (MQ=255)                                                                                                                                                                                                   aGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGCGGAAGGGTTAAc                                                 >  2:348253/1‑251 (MQ=255)                                                                                                                                                                                                         cACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCtt                                           <  1:259983/251‑1 (MQ=255)                                                                                                                                                                                                          aCGAGAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGG‑AATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTc                                         <  1:70707/251‑1 (MQ=255)                                                                                                                                                                                                             aaaaCTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCTCCTGTCGCGACAATCAGGTCGAATTCTTGCTGGTGTGGCCGAATCGCCTCCAGCACCTCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCGTCGTGTTTTTGTGCAAACAGG‑TGAGCGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCCCCAGCCACAGGAAGGGTTAACAGGCTTTCCa                                       <  1:697659/251‑1 (MQ=255)                                                                                                                                                                                                              aaaCTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCa                                       >  1:280318/1‑250 (MQ=255)                                                                                                                                                                                                              aaaCATGCGATGCCTGCGGGGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGTTAAATCACCTTCCTCGACAAGCAGGTCGAATGCGTGCTGGTGTGGCCGAGTCTCCTCCAGCACCGCCTGGTAACTCTCCCAGGCGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCATAGAGG‑TTAGTGTCGGTAATTTGTAAATTCCTGACTCTGGCCTCACGAGCCAGAGGAAGGGTTAGCAGGCTTTCCaa                                      <  2:702807/251‑1 (MQ=255)                                                                                                                                                                                                                         gCCTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCtt                           >  1:231799/1‑251 (MQ=255)                                                                                                                                                                                                                           cTTCAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCCCCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCtt                           >  1:610468/1‑249 (MQ=255)                                                                                                                                                                                                                              cAGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGtt                      <  1:615342/251‑1 (MQ=255)                                                                                                                                                                                                                               aGCGAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGCCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGCCTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGttt                     <  1:348253/251‑1 (MQ=255)                                                                                                                                                                                                                                  gAAATGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCac                  <  1:526016/251‑1 (MQ=255)                                                                                                                                                                                                                                     aTGCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAACTGGTGTCCTTAGGTTTCacgac               >  1:72956/1‑251 (MQ=255)                                                                                                                                                                                                                                       gCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCacgacg              <  2:38590/250‑1 (MQ=255)                                                                                                                                                                                                                                       gCTGATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGc             <  1:50722/251‑1 (MQ=255)                                                                                                                                                                                                                                        ctgctatcccGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGC‑GAATCGCCTCCAGCACCGCCTGGTAAATCTCCCCGGTGTTTACCCCTAACAGGGCTTCGGGCTTTTGTGCAAACAGGGTG‑GGGTCGGTAATTTTTAAAATTCTGGCTCTGGCCTCCCCAGCCCGGGGGAGGGTTTACAAGCTTTCCAAATGGTGTCCTTTGGTTTCACGACGCtt           >  2:395991/9‑250 (MQ=255)                                                                                                                                                                                                                                        cTGATAGGACGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCt            >  2:636198/1‑251 (MQ=255)                                                                                                                                                                                                                                          gATAGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCta           >  1:206084/1‑250 (MQ=255)                                                                                                                                                                                                                                             aGGCCGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaataa       <  2:207578/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cGCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCg   <  1:481094/251‑1 (MQ=255)                                                                                                                                                                                                                                                  gCAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCgg  >  1:714849/1‑251 (MQ=255)                                                                                                                                                                                                                                                   cAGAGAATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCgg  >  2:398109/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      GGCCAGTTTACGTTCCAGCCACTCAAGCTGAAACTCGCTCAGCTCACCGTGCGGCACGCCAAACACCTGGCTATCCAGCAACAGGATTTGCCATTGCTCACCAATAAACACGCGCTTCGCCGGGGAGATACCCGCATCCTGTAACGCGCTGTACATCGCGGGCTGGAAATCGTGGTTGCCCGGCAGCCAGACGCAGGGCGCACGAAAACTTGCGATGCCTTCAGCGAAATGCTGATAGGCCGCAGAGGATTGATCCTGCGCTAAATCACCTGTCGCGACAATCAGGTCGAATTCGTGCTGGTGTGGCCGAATCGCCTCCAGCACCGCCTGGTAACTCTCCCAGGTGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGG‑TGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGG  >  NC_000913/3176378‑3176869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑