Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,121,033	T→C	I83V (ATT→GTT)	glcA ←	glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,121,033	0	T	C	100.0%	196.7 / NA	55	I83V (ATT→GTT)	glcA	glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/34);  total (21/34)

TTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAATCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAG  >  NC_000913/3120789‑3121271                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               ttGGCATTCAGACACACCCCCGCCGCGTATAACGGTTTGAACCCCACCCCCACCACCAGCGCACCGTTAATCGCCACCGGCGCACCAAAGCCATCCGCCCCTTCCTGCACC‑GCACCAAAGGAGAAACCAATCAGTAACCCCTGCAAACGTTG‑ATCTTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCGGGCAACGTTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACg                                                                                                                                                                                                                                           <  1:367533/250‑1 (MQ=255)  tGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCt                                                                                                                                                                                                                                         >  1:5476/1‑251 (MQ=255)  gggCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAACCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCt                                                                                                                                                                                                                                         <  1:614908/250‑1 (MQ=255)     aaaTCAGACCCAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCCCCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTAtt                                                                                                                                                                                                                                      <  1:247815/250‑1 (MQ=255)      aaTCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGTTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTg                                                                                                                                                                                                                                     <  1:422973/251‑1 (MQ=255)         cAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGcc                                                                                                                                                                                                                                  >  1:583248/1‑251 (MQ=255)           gACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCAt                                                                                                                                                                                                                                >  1:606739/1‑251 (MQ=255)            acccaGCCCCGCCGGGTATATCGGTTGGAAGCCCAGGCCCACCAGCAGCGCCCCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCCTCCAGCAAC‑GCACCAAAGGAGAAACTAATCAGTAACTCCTGCAAACGCTG‑ATCGTCGGTGAGGGAGATAACCGGGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCGATTGGCCATa                                                                                                                                                                                                                               <  1:497888/248‑1 (MQ=255)            acccaGCCCCGCCGCGTAGAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAACCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ACCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATa                                                                                                                                                                                                                               <  1:153781/248‑1 (MQ=255)             cacaGCCCCCCCGCGTATAACTGTTTGAAGCCAAGGCCCACCAGCCGCGCACCGGTAAACGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCACCAAC‑GCCCCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATACCCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGAAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATaa                                                                                                                                                                                                                              <  1:329067/251‑1 (MQ=255)                      cccgcgaaaaaCGGTTGGAACCCCAGGCCCACCGGCAGCGCACGGGTAACCGCCACCGGCGCACCAAACCCAGCCGCCCCTTCCAGCAAC‑GCACCAAAGGAGAACCCAATCAGAAACACCGGCAAACGCTG‑ACCGTCGGTGATGGAGATAACCGAGCTGCGGAAAATATCGACCTGCCCGCGGGCAACGGTTAA‑TTTATACAGAAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCataaat                                                                                                                                                                                                                     <  2:634169/242‑1 (MQ=255)                          cgTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAg                                                                                                                                                                                                                 <  1:23883/251‑1 (MQ=255)                                  ggTTTGAAGCCCAGGCCCCCCAGCAGCGCCCCGGTAACCGCCACCGGCGCACCAAAGCCAGCCGCCCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGcc                                                                                                                                                                                                         <  1:134813/251‑1 (MQ=255)                                   gTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAACCGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGccc                                                                                                                                                                                                        <  1:234507/251‑1 (MQ=255)                                                gCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAAAGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATg                                                                                                                                                                                           >  2:693300/1‑251 (MQ=255)                                                          ccccacccGGTAACCGCCACCGGCGCACAAAAGCCGGCCGTCCCTTCCA‑CAACCGCACAAAAGGAGAACCAAAGCATTACCCCCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATACCGAACGGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CCCCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCAGATCAAt                                                                                                                                                                                 <  2:503934/245‑1 (MQ=255)                                                          agcaccccGGTAATCGCCACCGGCGCCCCAAAGCCCGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGAGGGAGATAACCGAGCTGCGTATAATATCGAACTCCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAAt                                                                                                                                                                                 <  2:717724/245‑1 (MQ=255)                                                                               ggCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAatat                                                                                                                                                            >  2:626949/1‑251 (MQ=255)                                                                                     ccAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAAt                                                                                                                                                      >  2:76140/1‑251 (MQ=255)                                                                                      cAAAGCCAGCCGCTCCTTCCAGCAAC‑GCCCCAAAGGAGAAACCAATCAGTAACCCCTGCAAACGCGG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACGGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATc                                                                                                                                                     <  1:218439/251‑1 (MQ=255)                                                                                      cAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAACCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTATAACGGCGAATATTGCAAt                                                                                                                                                      >  1:550455/1‑250 (MQ=255)                                                                                            cagccGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGgata                                                                                                                                               <  1:693300/251‑1 (MQ=255)                                                                                                  cctccttacagaacggccacAAAGGAGAAACCAATCAGTAACCCCTGCAAACGCTG‑GTCTTGTGGGATGGAAAAAACCCAGCGGGGGAAAAAATCGACCTGCCCGCTGCAAACGGTTAA‑TTAATCCAGAAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGataa                                                                                                                                          <  2:463163/232‑1 (MQ=255)                                                                                                    ccTTCAAGAAAC‑GCACCAAAGGAAAAACCAATCAGTAACACCTGCAAACG‑TGTATCGTGGGTGATGGAAATAACCGAGCTGCGGATAATATCTAACGGCCCGCTGGCAACGGTTAA‑TTTAAACAGGAC‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAatat                                                                                                                                       <  2:542070/251‑1 (MQ=255)                                                                                                      ttCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAAtata                                                                                                                                      >  1:161384/1‑250 (MQ=255)                                                                                                       tCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGCGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAAtata                                                                                                                                      >  1:313426/1‑249 (MQ=255)                                                                                                       cccAGCAAC‑GCCCCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGTAGATAACGGAGCTGCGTATAATATCGAACGGCCCGCTGGCAACTGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAAGGCCATATCAATCGGCAGTTTAAAAGCGAATATTGCAATCAGGATAGATAATATAAg                                                                                                                                    <  2:717368/250‑1 (MQ=255)                                                                                                         cAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAggg                                                                                                                                  <  1:601153/251‑1 (MQ=255)                                                                                                           gCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGtt                                                                                                                                <  1:76140/251‑1 (MQ=255)                                                                                                             aaC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTAt                                                                                                                              >  1:65913/1‑251 (MQ=255)                                                                                                                    caaaaGGAAAAACAAACCAGTACCACCTGAAAACGCTG‑ATCGTCGGTGAGGGAGATAACCGAGCTGCGGAAAAAACCGACCTGCCCGCTGGCAACGGTTAA‑TTTATACAGAAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTcc                                                                                                                        <  2:120520/249‑1 (MQ=255)                                                                                                                         ggagaacacAATCATTAACACTTGCAAACGCTG‑ATCTTCGGTGATGGAGATAACCGAGTGGCGGAAAATATCGAACGGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CCCCGCCGCGACAATAACCCACGCTATTGGCCATAATCCAGAAATAAAGCCATAGCCCGCAGCATCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGa                                                                                                                   <  1:92453/243‑1 (MQ=255)                                                                                                                                 cAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGCTGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGACCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCCGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCt                                                                                                           >  2:673028/1‑251 (MQ=255)                                                                                                                                    tCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTc                                                                                                        >  1:198309/1‑251 (MQ=255)                                                                                                                                    tCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGACCTTTc                                                                                                        >  1:242938/1‑251 (MQ=255)                                                                                                                                        tAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGAc                                                                                                    >  2:591559/1‑251 (MQ=255)                                                                                                                                         aaccccTGCAACCGCGG‑ATCGTCGGTGAGGGAGATAACCGATCGGCGGATAATATCGAACTGCCCGCGGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGAc                                                                                                    <  2:518115/246‑1 (MQ=255)                                                                                                                                         aaCACCTACAAACGCGG‑ATCGGCGGTGATGGAGATAACCGAGCTGCGGATAATACCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGAc                                                                                                    <  2:662018/250‑1 (MQ=255)                                                                                                                                                   aaCGCTG‑ACCTTCGGTGATGGAGATACCCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGTTTAA‑TTTATACAGGAA‑AACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACcgcg                                                                                         <  2:5476/251‑1 (MQ=255)                                                                                                                                                                  gTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACgaaga                                                                            >  2:401217/1‑250 (MQ=255)                                                                                                                                                                    atggtaaaataCCGAGCGGCGGATAATAGTAAACGGCCCTCTGGCAA‑GGTTAAATTTATA‑ACGAATCACCGCCGCGACAATAACCCTCGATAGTGGCCATAATCCAGAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCTGCATTTTAAAGGCGAATATTGCAATCAGGATATATAATATAAGGGTTATTGCGCCAGCGACATTTCGTTTCAGCCGTAACACCTCGAGGGCAACgatgaaga                                                                         <  2:694009/241‑1 (MQ=255)                                                                                                                                                                               ccGAGCTGAGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTAATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCggg                                                              <  1:442613/251‑1 (MQ=255)                                                                                                                                                                                     tGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAgg                                                        >  1:13391/1‑251 (MQ=255)                                                                                                                                                                                       aggaaaaaatcgagttGCCCGCGGGCACCTGTTAA‑TATATCCAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGGAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGc                                                      <  2:515270/236‑1 (MQ=255)                                                                                                                                                                                              aatCGAACGGCCCGCGGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCaga                                               <  2:187332/250‑1 (MQ=255)                                                                                                                                                                                               atCGAACTGACCGCTGGACACGGTTAA‑TTTATACAGGAC‑CACCGCCACGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAACGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCag                                                >  2:490346/1‑249 (MQ=255)                                                                                                                                                                                                  gAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATCAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGCCGACCAGAGCgg                                           >  1:372810/1‑251 (MQ=255)                                                                                                                                                                                                        cccctccacgacgctctcccgatctcAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGc                                      <  2:317120/226‑1 (MQ=255)                                                                                                                                                                                                            cTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGCTCAGGGCGACCAGAGCGGATAGcccc                                   >  2:465680/1‑249 (MQ=255)                                                                                                                                                                                                              ggCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGt                                <  1:591559/250‑1 (MQ=255)                                                                                                                                                                                                                cAACGCTTAA‑TTTCTACCGGAA‑CACCGCCGCGACACTACCCCCCGCTATTGGCCTTAATCCATAAATAAAGACATCGCCCGCAGCCCCAACTGCCATATCAATCGGCATTTTAAAGGCGAATCTTGCAATCGGGATAGATACTATAAGGGTTATTGCTCCAGAGACATGTCCTTTCAGACGTAATACCGCGAGTGCAAAGAAGAAGAATATTATCGGGTTCAGGTCGACCAGAGCGGATAGCCCCAGtctt                             >  2:310725/1‑249 (MQ=255)                                                                                                                                                                                                                      taaa‑TTTATACAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCACAGTCCTCCCAt                        <  1:626949/248‑1 (MQ=255)                                                                                                                                                                                                                                 cAGGAA‑CACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGc                                      >  1:317120/1‑226 (MQ=255)                                                                                                                                                                                                                                           cgccgcGACAATAACCCACGCTATGGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCGCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGCATATTATCGGGATCAGGGCGACCAGAGCGTATAGCCCCAGTCCTCCCATCTGCATATGCATGTGGGtac    <  2:447564/251‑3 (MQ=255)                                                                                                                                                                                                                                             ccgcGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAg  <  2:606739/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               TTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAAC‑GCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTG‑ATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAA‑TTTATACAGGAA‑CACCGCCGCGACAATAATCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATATACATTTGGGTCCAG  >  NC_000913/3120789‑3121271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑