Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,887	G→A	S152N (AGC→AAC)	mltC →	membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,887	0	G	A	100.0%	350.6 / NA	96	S152N (AGC→AAC)	mltC	membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (50/46);  total (50/46)

ATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATT  >  NC_000913/3104645‑3105130                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    atatCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTcg                                                                                                                                                                                                                                             <  1:579611/251‑1 (MQ=255)   atCAGACCCGCAGCCACATCACCTTCGATGACGGTACGAATACTATCGAAACCATCGCCGGGACAGAACCTGACGCGCATTTGCGCCGGGCAATTATCCAAAAGTTATTGATGGGGGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGTTGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGATCGCGAAAACAACTTcgcg                                                                                                                                                                                                                                           >  1:394826/1‑251 (MQ=255)   atCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTcgc                                                                                                                                                                                                                                            <  2:403894/250‑1 (MQ=255)    tCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTAAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCgg                                                                                                                                                                                                                                          >  1:100912/1‑251 (MQ=255)     cAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCgg                                                                                                                                                                                                                                          <  2:368759/250‑1 (MQ=255)     cAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGa                                                                                                                                                                                                                                         <  1:435210/251‑1 (MQ=255)          ccGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATc                                                                                                                                                                                                                                    <  1:513114/251‑1 (MQ=255)              aGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATctgct                                                                                                                                                                                                                                <  1:132444/251‑1 (MQ=255)               gCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATctgctg                                                                                                                                                                                                                               >  1:629468/1‑251 (MQ=255)                ccACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGa                                                                                                                                                                                                                              <  1:104448/251‑1 (MQ=255)                   caTCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGCTTATctgctggaa                                                                                                                                                                                                                            >  1:725087/1‑247 (MQ=255)                      catcTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAAc                                                                                                                                                                                                                         <  2:521134/247‑1 (MQ=255)                       aaCTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCg                                                                                                                                                                                                                       <  1:51746/251‑1 (MQ=255)                       aaCTTAGATGACGGTACGCTTACTATCGAAAAAATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAACCCg                                                                                                                                                                                                                       >  1:590313/1‑251 (MQ=255)                            cGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGAtt                                                                                                                                                                                                                                       >  2:142138/1‑230 (MQ=255)                            cGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGAtt                                                                                                                                                                                                                                       <  1:142138/230‑1 (MQ=255)                                gACGGTACGATTACTATCGCAACAATCGCCGGGACAGAAACTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCAGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGAAAACAACTTCGCGGATTATCTGCTGAAACACCGTCTGAag                                                                                                                                                                                                                >  2:57564/1‑249 (MQ=255)                                               aTCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTGTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGATCCGCAGCAACGGGc                                                                                                                                                                                                <  2:494242/250‑1 (MQ=255)                                               aTCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGGTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTGCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACgggt                                                                                                                                                                                                <  2:521252/250‑2 (MQ=255)                                                tCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTg                                                                                                                                                                                              >  2:241631/1‑251 (MQ=255)                                                  gAAACCCTCGCCGGGACAGCACCTGCCGCGCATTTGCGCCGGGCAATTATCAAACCGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGAGCTGCg                                                                                                                                                                                            >  2:279498/1‑251 (MQ=255)                                                     aCCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTat                                                                                                                                                                                         >  2:527714/1‑251 (MQ=255)                                                      ccATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTatc                                                                                                                                                                                        >  1:396906/1‑251 (MQ=255)                                                           gCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCt                                                                                                                                                                                    >  1:405748/1‑250 (MQ=255)                                                           gCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCt                                                                                                                                                                                    >  2:160942/1‑250 (MQ=255)                                                            ccGGGCCAGAACCTGCCGCGCCTTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTAc                                                                                                                                                                                  >  2:337476/1‑251 (MQ=255)                                                            ccGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTAc                                                                                                                                                                                  <  2:149801/251‑1 (MQ=255)                                                             cGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTAc                                                                                                                                                                                  >  1:224218/1‑250 (MQ=255)                                                                    gAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAACCGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCAc                                                                                                                                                                          >  2:453481/1‑251 (MQ=255)                                                                          gCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCAtt                                                                                                                                                                      <  1:198469/249‑1 (MQ=255)                                                                                cATTTGCGCCGGGCAATTATCAAAACGTTATTGTTGGGTGCCGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGt                                                                                                                                                              >  2:360690/1‑251 (MQ=255)                                                                                  tttGCGCCGGGCAATTATCAACACGTTATTGATGGATGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGTTATTGCGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGc                                                                                                                                                            >  2:105100/1‑251 (MQ=255)                                                                                  tttGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGc                                                                                                                                                            >  1:517248/1‑251 (MQ=255)                                                                                      cgcCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGa                                                                                                                                                         >  2:268089/1‑250 (MQ=255)                                                                                         cGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTACCATGGTGCCGAacc                                                                                                                                                      >  1:587644/1‑250 (MQ=255)                                                                                         cGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAacca                                                                                                                                                     <  1:531449/251‑1 (MQ=255)                                                                                                  aTCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATaa                                                                                                                                            <  2:676919/251‑1 (MQ=255)                                                                                                    cAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGTCGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAAc                                                                                                                                          <  1:391563/251‑1 (MQ=255)                                                                                                           ttATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTgcgc                                                                                                                                    >  2:213800/1‑250 (MQ=255)                                                                                                             aTTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGcac                                                                                                                                  <  2:344904/250‑1 (MQ=255)                                                                                                                gATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTTCCGAACCACCTTGATAAACGTGCGCACaaa                                                                                                                               <  1:600399/250‑1 (MQ=255)                                                                                                                 aTGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGCTATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAat                                                                                                                              >  2:690354/1‑250 (MQ=255)                                                                                                                       gACGAGCCGAGTACGGTCGATCTCTATGCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGTACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACAGGGTGCCGAACCACCTTGAGAAACTTGCGCACAAATATCTCg                                                                                                                        <  2:478597/250‑1 (MQ=255)                                                                                                                           aTCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTTCTGAAAAACCGGCTGAAGAGCCGCAGCAACGGGCTGCGTATCGTCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATg                                                                                                                   <  1:733720/251‑1 (MQ=255)                                                                                                                               gAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTcc                                                                                                               >  2:601209/1‑251 (MQ=255)                                                                                                                                aGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGACCCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATATGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCg                                                                                                              >  2:638247/1‑251 (MQ=255)                                                                                                                                aGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCg                                                                                                              >  1:430478/1‑251 (MQ=255)                                                                                                                                aGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCg                                                                                                              <  1:160942/251‑1 (MQ=255)                                                                                                                                         gATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTc                                                                                                     >  2:561128/1‑251 (MQ=255)                                                                                                                                          aTCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTc                                                                                                     >  1:490132/1‑250 (MQ=255)                                                                                                                                              ctATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACg                                                                                                  <  1:239366/249‑1 (MQ=255)                                                                                                                                              ctATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGGCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATAGCTCGGCATGGTCCGCCAGGCGTCa                                                                                                    <  2:725087/247‑1 (MQ=255)                                                                                                                                                   ccGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCTCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGGGCGCACAAATATCTCTGCATGGGCCGCCAGGCGTCACGTAAata                                                                                            <  2:699737/250‑1 (MQ=255)                                                                                                                                                   ccGACGTTGATGATATTACGATGTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGGCTGAAGAGCCGCAGCAACGGGCGGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGGGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAatat                                                                                           <  1:433525/251‑1 (MQ=255)                                                                                                                                                     gACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATgg                                                                                         <  2:504184/251‑1 (MQ=255)                                                                                                                                                          tgatgaTATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACCCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGtt                                                                                     <  1:549033/250‑1 (MQ=255)                                                                                                                                                               atatTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCACACAACTTCGCGGCTTATCTGCTGAAAAACCGTCTGCAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGAGTtgatg                                                                                 >  1:735399/1‑249 (MQ=255)                                                                                                                                                                 atTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCGCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGt                                                                              <  2:430478/250‑1 (MQ=255)                                                                                                                                                                    aCGATTTCGAACGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCCAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCCCGGAAATATGGCGTTGATGAGTCGCt                                                                          >  2:368792/1‑251 (MQ=255)                                                                                                                                                                    aCGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCt                                                                          <  2:229314/251‑1 (MQ=255)                                                                                                                                                                     cGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTg                                                                         <  1:642810/251‑1 (MQ=255)                                                                                                                                                                     cGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTg                                                                         >  2:394338/1‑251 (MQ=255)                                                                                                                                                                      gATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTg                                                                         >  1:170010/1‑250 (MQ=255)                                                                                                                                                                           cGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTg                                                                   >  1:114590/1‑251 (MQ=255)                                                                                                                                                                             aaaGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAAACACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAACTATGGCGTTGCTGAGTCGCTGATTCGGGc                                                                 >  2:651793/1‑251 (MQ=255)                                                                                                                                                                                 aaCCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGTCAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGCCACCATTAACATGGAGCCGAACCACCTTGATAAACGGGCGCACACATATCTCGGCATGGTCCGCCAGGCGTCACGGAATTATGGAGTTGATGATTCGCTGATTCTGGCAAtt                                                             >  2:694699/1‑251 (MQ=255)                                                                                                                                                                                   cctttcctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGTGACGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTAt                                                           >  2:244610/1‑251 (MQ=255)                                                                                                                                                                                   cctttcctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTAt                                                           >  2:307900/1‑251 (MQ=255)                                                                                                                                                                                     tttcctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTtga                                                                                   <  2:73096/225‑1 (MQ=255)                                                                                                                                                                                     tttcctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTtga                                                                                   >  1:73096/1‑225 (MQ=255)                                                                                                                                                                                      ttcctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGc                                                         >  1:687969/1‑250 (MQ=255)                                                                                                                                                                                       tcctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCa                                                        <  1:285015/250‑1 (MQ=255)                                                                                                                                                                                        cctttACGGTCAGGTGGTGGACAACACCGGGCAGCCTATTCGCTGGGAAGGTCGAGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGAGAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTAGGCAGa                                                      <  1:370187/251‑1 (MQ=255)                                                                                                                                                                                           ttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGAcc                                                    <  2:687969/250‑1 (MQ=255)                                                                                                                                                                                              cGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAACTATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAAt                                                >  2:409802/1‑251 (MQ=255)                                                                                                                                                                                              cGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAAt                                                >  2:378419/1‑251 (MQ=255)                                                                                                                                                                                              cGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAAt                                                <  2:629468/251‑1 (MQ=255)                                                                                                                                                                                                gTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGACAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCt                                              >  1:613035/1‑251 (MQ=255)                                                                                                                                                                                                   aGGTGCTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCCAACAACTTCGCGGATTATCTGCTGACAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAACTATGGCGTTGATGATTCGCTGATTCTGGCAATTATGCAGACCGCATCTTcc                                           >  2:472318/1‑251 (MQ=255)                                                                                                                                                                                                      tggtggACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCttt                                        >  2:213741/1‑251 (MQ=255)                                                                                                                                                                                                       ggtggACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCttt                                        >  1:439116/1‑250 (MQ=255)                                                                                                                                                                                                           gACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAAccc                                   <  1:690354/251‑1 (MQ=255)                                                                                                                                                                                                            acaacaCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCg                                  <  2:405748/251‑1 (MQ=255)                                                                                                                                                                                                             caacaCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCATGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGt                                 >  1:395330/1‑251 (MQ=255)                                                                                                                                                                                                             caacaCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCg                                  >  2:508427/1‑250 (MQ=255)                                                                                                                                                                                                             caacaCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGt                                 >  1:581061/1‑251 (MQ=255)                                                                                                                                                                                                              aacaCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTa                                <  2:396906/251‑1 (MQ=255)                                                                                                                                                                                                              aacaCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTGCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCGCGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTGCCTTGAACCCGTa                                <  2:363185/251‑1 (MQ=255)                                                                                                                                                                                                               acacCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTAt                               <  2:581061/251‑1 (MQ=255)                                                                                                                                                                                                                    gggCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGt                          <  1:337476/251‑1 (MQ=255)                                                                                                                                                                                                                     ggCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTc                         <  1:223511/251‑1 (MQ=255)                                                                                                                                                                                                                        aGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGCTAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGc                      >  2:379456/1‑251 (MQ=255)                                                                                                                                                                                                                            gATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGtt                  <  2:127939/251‑1 (MQ=255)                                                                                                                                                                                                                                    gggAAGGTCTCGCAAACAACTTCGCGGATTATGTGCTGAAAATCCATCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCGACAGCGTCTCCTTTAACATGGTGCCGAACCTCCTTGATAAACGGGCGCAAAAATATCCCGTCATGGTCCGCCAGGCGCCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAAGTAGGCTGACCGAATCTTTCTTTACCCCGTATGCGGTCAGCCGTTCCGATgc           <  1:40841/250‑1 (MQ=255)                                                                                                                                                                                                                                        aGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTgg      >  1:599843/1‑251 (MQ=255)                                                                                                                                                                                                                                            cgcgCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATAGCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGGCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGAtt  <  1:653135/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATT  >  NC_000913/3104645‑3105130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑