Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	466,148	G→A	P389S (CCA→TCA)	ybaE ←	uncharacterized protein YbaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	466,148	0	G	A	100.0%	192.5 / NA	59	P389S (CCA→TCA)	ybaE	uncharacterized protein YbaE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/32);  total (27/32)

TTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCT‑CCGGTTTGCCGATGGTGATTTGCACGGGATGCCGACAACTGGTTCCCAAATCTT  >  NC_000913/465906‑466390                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ttCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGGCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCACGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATg                                                                                                                                                                                                                                             >  1:652659/1‑251 (MQ=255)   ccGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGc                                                                                                                                                                                                                                            <  1:215600/250‑1 (MQ=255)      gTGCTTCGCCAATTAATCTGTAGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGCCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATg                                                                                                                                                                                                                                         >  2:144457/1‑250 (MQ=255)                 aTTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGAt                                                                                                                                                                                                                             >  1:204874/1‑251 (MQ=255)                  ttAATCTGTCGCCCAACATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTggtc                                                                                                                                                                                                                            >  1:625695/1‑248 (MQ=255)                    aaTCTGTCGCCCATCATGAGGGCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCa                                                                                                                                                                                                                           <  1:332454/250‑1 (MQ=255)                         gTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAgg                                                                                                                                                                                                                         <  2:537060/247‑1 (MQ=255)                         gTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGtt                                                                                                                                                                                                                       <  2:264810/249‑1 (MQ=255)                             cccATCATGAGGTCTGCGGGTGCCTGTAGGGTCGGGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTcc                                                                                                                                                                                                                 <  2:212756/251‑1 (MQ=255)                                atcatGAGGTCTGCGTGTGCCTGTAGGTTCGTGTCGTCCCAGTTTTTTGCGGTATGAAAAATAATTGTGAGTTCAACGCCTTCCGCTGCCAGTGGCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGGGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTTCCTGCCAATGCGGAATAGTCCAGCCGGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGAc                                                                                                                                                                                                              <  2:50581/251‑1 (MQ=255)                                      agggcTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTaa                                                                                                                                                                                                        <  1:627959/247‑1 (MQ=255)                                       ggTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAAc                                                                                                                                                                                                       <  1:452799/251‑1 (MQ=255)                                                    cTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGGTTTCTCCGACTTCTAAAGGTTGTAATAAAc                                                                                                                                                                                          >  1:401982/1‑251 (MQ=255)                                                      gTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCg                                                                                                                                                                                        >  1:128365/1‑251 (MQ=255)                                                                        atttttgtGCGTTATTAAAACGAATGGTGATTTCATAGCCTACCGCTGCCATTGTCGCCTGTAGGAGTTCTGCCATGTTATGAAGTTCTATCGGTAGCTGATAAACCAGCGTCAAGTTTTTCGGTAGTTTGACTTCATCCGGTCCCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTGGTAATAAACCGGATTGATGAATAATGgat                                                                                                                                                                      <  2:101887/249‑2 (MQ=255)                                                                        aGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATggcg                                                                                                                                                                      >  1:522328/1‑249 (MQ=255)                                                                        aGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGGGATCAGGGTTTCTCCGCCTTCTCAAGGTTGTAATAAACCGGATTTATGaataattg                                                                                                                                                                        >  2:439217/1‑247 (MQ=255)                                                                                 cGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGGTCAGGGTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCAAtttt                                                                                                                                                             >  2:28244/1‑251 (MQ=255)                                                                                 cGTTAGGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACtttt                                                                                                                                                             <  1:240656/251‑1 (MQ=255)                                                                                   ttATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTcg                                                                                                                                                           >  1:53156/1‑251 (MQ=255)                                                                                           aataatTGTGAGTTAACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGcc                                                                                                                                                   <  2:237865/251‑1 (MQ=255)                                                                                                 tgtgAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAgg                                                                                                                                              <  2:253540/250‑1 (MQ=255)                                                                                                          acaGCCTTCAGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGCTAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGATTTATCCGACTTTTAACGTTTGTAATCAACCGGGTTAATTAATAATGGAGAACACTTTTGGCGCCTGCAAGCGGGAGAGTCggg                                                                                                                                    >  2:581584/1‑251 (MQ=255)                                                                                                           caGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCgggg                                                                                                                                   <  2:556941/251‑1 (MQ=255)                                                                                                           caGCCTTCCGCTGCCAGTGTCGCATGTAGGCGTTCTGCCATGTTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTAAAGGTTTTCGGTAGTTTGACTTCATCCGGTCCCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCgggg                                                                                                                                   <  2:8105/251‑1 (MQ=255)                                                                                                               cTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCCGGTTTTCTCCGCCTTCTTACGTTTGTAATAAACCGGATTGTTGTATAATGGGGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCgggggatt                                                                                                                               >  1:173824/1‑247 (MQ=255)                                                                                                                   cGCTGCCAGTGTCGCCTGTAGGCGGTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTgc                                                                                                                           <  1:147628/251‑1 (MQ=255)                                                                                                                         cagggtCGCCTGTGGGCGTTCGGCCATGGTATGAAGTTTTTTCGGTAGGTAATAAACCAGCGTCAAGGTTTTCGGTAGTTTGCCTTCACCCGGTACCCGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACg                                                                                                                     <  2:522328/247‑1 (MQ=255)                                                                                                                         cAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACg                                                                                                                     <  1:714239/251‑1 (MQ=255)                                                                                                                           gtgtCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCCATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTATCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCCGCCCGAGGGAGAGTCGGGGACTTTTTCGCCACGTc                                                                                                                   >  1:726809/1‑251 (MQ=255)                                                                                                                              tCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTAGGTAGATTGCCTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTATACTAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGGCTTTTGCGCAAAGTCaa                                                                                                                 >  1:725808/1‑250 (MQ=255)                                                                                                                                gCCTGTAGGCGTTCTGCCAGGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATa                                                                                                              <  2:199754/251‑1 (MQ=255)                                                                                                                                   tGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCCGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGGTTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACCTTTGGGCAACGTCAAATAGc                                                                                                            >  2:618836/1‑250 (MQ=255)                                                                                                                                               gCCATGGTATGAAGTGCTACCGGTAGGTGAGAAACCAGTGGCAAGGTTTTCGGTAGTTGTACATAAACCGGTACCTGCCAATGCGGAATAGTCCAGCATGACAGTAATGCATGACTGGCTGTTATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTAATGAATAATGGAGATCACTTTTCTCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGAGCAGCGTCAAAAAGCAAAAACCTaaa                                                                                                 <  2:652659/249‑1 (MQ=255)                                                                                                                                                  aTGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTACGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGAt                                                                                            <  1:28244/251‑1 (MQ=255)                                                                                                                                                    ggTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGc                                                                                          >  1:530585/1‑251 (MQ=255)                                                                                                                                                     gTATGACGTTCTATCGTTTGGTGATAAACCAGCTTCAAGTTTTGCGGGAGTGTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCTAGCCGGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTGCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGTGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGcc                                                                                         <  1:677700/251‑1 (MQ=255)                                                                                                                                                      tATGAAGTTCTATCGGTAGGTGCTAAACCCGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGACCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTTCCGTATGTTATAAACCGGATTGATAAATAATGGAGCTCCCATTTCCCGCCAGCCCGAGGGCAAGGCGGGGGCTTTTGCGCCACCACAAATAGCAAAAACCTACACTggagcc                                                                                         >  1:138800/1‑245 (MQ=255)                                                                                                                                                       aTGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGGTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGGCTTTTGCGCAACGTCAAATAGCCAAAACCTAAACTGATGCCGc                                                                                       >  1:508594/1‑251 (MQ=255)                                                                                                                                                                    ggTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATTGAGATCACTTTTCGCGCCTGCCAGAGGGGGAGTCGGGGGCTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTgact                                                                          >  2:610279/1‑248 (MQ=255)                                                                                                                                                                    ggTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCt                                                                          <  1:331938/251‑1 (MQ=255)                                                                                                                                                                       aGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGAc                                                                       <  1:404928/251‑1 (MQ=255)                                                                                                                                                                            aTAAACCAGCGTCAAGGTTTTCGGGAGTTTGACTCCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTGCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTTACCTGGCTGACCCGtt                                                                  <  2:138800/251‑1 (MQ=255)                                                                                                                                                                                            gTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGGGATCCCTTTTCGCGCCTGCCAGAGGGAGGGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAAACTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCT‑CCgggtt                                                 >  1:473189/1‑248 (MQ=255)                                                                                                                                                                                              tgtCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGTTGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGTCCTGGCTGACCCGGTGCAGCTCCT‑CCGGTTTg                                                <  1:638473/248‑1 (MQ=255)                                                                                                                                                                                                  ggTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAAATGCTGCCGCTCCTGACCTTGCTGGCCCCTTGCAGCTCCT‑CCCGTTTGccc                                             >  2:203692/1‑248 (MQ=255)                                                                                                                                                                                                        gtgccTTCACCCGGAAGCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCGCCGACTTCTAACGTTTGTAAGAAACCGGATTGATGAATAATGGAGAACACTTTTCGCGCCGGCCAGAGGGAGAGTCGGGGGCTTGTGCGCAACGTCAAACTGCAAACACCTAAACGGATGCCTCGTCTGACCTGGCTGAGCCGTGGCAGCTTCT‑ACGGTCGGCCTATGGTGa                                      <  2:48755/246‑1 (MQ=255)                                                                                                                                                                                                         tGACTTCATCCGGTCCCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCGACTGACCTGGCTGACCCGTTGCAGCTCCT‑CCGGTTTGCCGATGGTGAt                                     <  1:610279/250‑1 (MQ=255)                                                                                                                                                                                                         tGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAAATGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCT‑CCGGTTTGCCGATGGTGAtt                                    >  1:590672/1‑251 (MQ=255)                                                                                                                                                                                                              tCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAAGGGAGATCTCTGTTCGCGCCTGCCATAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTTACCTGGCTGACCCGTTGCAGCTCCT‑CCGGTTTGCCGATGGTGATTTGc                                 <  1:436926/249‑1 (MQ=255)                                                                                                                                                                                                                aTCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCTCCTGCCAGAGGGAGAGTCGGGGGCTTTTGCGCCACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCCGCCCCT‑CCCGTTTGCCGATTGGGAtttttaccg                             >  2:190355/1‑245 (MQ=255)                                                                                                                                                                                                                     gTACCTGCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGCCTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGGCAAATAGCAAAAAACTAAACTGATGCCGCCACTGACCTGGCTGACCCGGTGCAGCTCCT‑CCGGTTTTCCGCTGGTGGTTTGCACggggggc                        >  2:166541/1‑247 (MQ=255)                                                                                                                                                                                                                           gCCAATGCGGAATAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCT‑CCGGTTTGCCGATGGTGATTTGCACGGGATGCCGAc                    <  1:552213/249‑1 (MQ=255)                                                                                                                                                                                                                                     aaTAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCCACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGGTGCAGCTCCT‑CCGGTTTGCCGATGGTGGTTTGCACGGGGTGCCGACAACTGGTTcccc        >  1:322862/1‑250 (MQ=255)                                                                                                                                                                                                                                      aTAGTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCT‑CCGGTTTGCCGATGGTGATTTGCACGGGATGCCGACAACTGGTTCCCaa       <  1:558254/251‑1 (MQ=255)                                                                                                                                                                                                                                        aGTCCAGCCTGACAGTCATGCATGACTGGCGGTGATCAGGTTTTCTCCGGCTTCTAACGGTTGTAATACACCGGATTGAGGATTAATTGAGATCACTTTTCGTGCCTGCCAAAGGGTGAGTCGGGGGCTTCTGTGGAACGCCACATAACAACAAACTAACCTGGTTCCGCGCCTGCCCTCGCTTACCCCTTGCAGATCCTACGGGTTTCCCGTGGGTG‑TTTGCACGGGATGACGGCAACAGGTTCCCCAAt     >  2:291892/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCT‑CCGGTTTGCCGATGGTGATTTGCACGGGATGCCGACTACGGGTTCCCAAATc    <  2:726809/251‑1 (MQ=255)                                                                                                                                                                                                                                          tCCAGCCTGACAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCT‑CCGGTTTGCCGATGGTGATTTGCCCGGGGTGCCGACAACTGGTTCCCAAATCt   >  1:59356/1‑251 (MQ=255)                                                                                                                                                                                                                                           ccAGCCTGACAGTAATGCATGACTGGCGGTGAGCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCT‑CCGGTTTGCCGATGGTGATTTGCACGGGATGCCGACAACTGGTTCCCAAATCtt  <  2:173824/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    TTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGACTTCTAACGTTTGTAATAAACCGGATTGATGAATAATGGAGATCACTTTTCGCGCCTGCCAGAGGGAGAGTCGGGGACTTTTGCGCAACGTCAAATAGCAAAAACCTAAACTGATGCCGCTACTGACCTGGCTGACCCGTTGCAGCTCCT‑CCGGTTTGCCGATGGTGATTTGCACGGGATGCCGACAACTGGTTCCCAAATCTT  >  NC_000913/465906‑466390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑