Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,856,243	A→G	T636A (ACG→GCG)	fhlA →	DNA‑binding transcriptional activator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,856,243	0	A	G	100.0%	196.9 / NA	58	T636A (ACG→GCG)	fhlA	DNA‑binding transcriptional activator FhlA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/29);  total (29/29)

GAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATT  >  NC_000913/2856006‑2856481                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               gAAGATATTCCGTTGCTGGCGAAAGCCCTTACCTTCAAAATTGCCCGCCGTCTGGGGCGCAATATCGACACCATTCCTGCCGAGACGCTGCGCACCGTGAGCAACATGGAGTGGCCGGTTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACCCGGTAACGGGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGACCCTGAAACTCCTCCTGCCGCAGCGGTTGTCGCCCt                                                                                                                                                                                                                                   <  2:145530/251‑1 (MQ=255)   aGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTgg                                                                                                                                                                                                                                 >  1:438184/1‑251 (MQ=255)     atatTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTgg                                                                                                                                                                                                                                 >  1:593006/1‑249 (MQ=255)     atatTCCGCGGCCGGCGAAAGCCTTTACCTTCAAACTTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAg                                                                                                                                                                                                                               <  1:473008/251‑1 (MQ=255)          ccGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCg                                                                                                                                                                                                                           <  1:582660/250‑1 (MQ=255)                         gCCTTTACCTTCAAAATGGCCCGGCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTGTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGtt                                                                                                                                                                                                           <  2:146440/251‑1 (MQ=255)                          ccTTTACCTTCAAACTTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGCACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTg                                                                                                                                                                                                          >  2:322846/1‑251 (MQ=255)                               tccgtCAAAATGGCCCGTCGTTTGGGGCGAATTTTCGACATCTTTCCTGCCGAGACGCTGGGCTCTTTGATCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACTTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCGTTGCCAGATTTTGTTTTGCAGGAACCTGAAACGCCGCTGGCCGCAGCGGTTGTCGCCCTGGAGTGCGAATAGGAATATCAGGTGATtg                                                                                                                                                                                                      <  2:145840/246‑1 (MQ=255)                                    aaaaaTTGCCCGTCGTCTGGGGCGCAATACCGACGTCGTTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATGGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCAGTGCCAGATATTGTTTTATCGGAAGCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTgcgct                                                                                                                                                                                                <  2:409654/250‑2 (MQ=255)                                            cccGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGGACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGACTATCAGTTGATTGTGCGCGTGCTgaa                                                                                                                                                                                         >  1:75890/1‑250 (MQ=255)                                            cccGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTgaa                                                                                                                                                                                         >  1:696319/1‑250 (MQ=255)                                              cgtcgtCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTACCGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGCGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTgaa                                                                                                                                                                                         >  2:696766/1‑248 (MQ=255)                                              cgtcgtCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCTTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTgaaaga                                                                                                                                                                                      <  1:309361/251‑1 (MQ=255)                                                  gtcTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGACAGAAAcc                                                                                                                                                                                  >  1:115444/1‑251 (MQ=255)                                                    cTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGAGCACCTTGCGCAACATGGAGTGGACGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCa                                                                                                                                                                                 >  2:459301/1‑250 (MQ=255)                                                             aaTATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGCGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGt                                                                                                                                                                       >  2:37093/1‑251 (MQ=255)                                                                atCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGc                                                                                                                                                                    <  1:47000/251‑1 (MQ=255)                                                                               gCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGGGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCATAAAGgcgc                                                                                                                                                     >  2:523768/1‑251 (MQ=255)                                                                                      cGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCaa                                                                                                                                              <  1:655903/251‑1 (MQ=255)                                                                                         tGCGCCCCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGCTATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGt                                                                                                                                           >  2:370228/1‑251 (MQ=255)                                                                                           cgcACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCt                                                                                                                                         <  1:370228/251‑1 (MQ=255)                                                                                                    aGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGa                                                                                                                                 >  2:249244/1‑250 (MQ=255)                                                                                                        aCATGGAGTGGCCGGGTAACGTACGCGAACTGAAAAACGTCAGTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTGTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGGTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGc                                                                                                                            <  2:609736/251‑1 (MQ=255)                                                                                                         cATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCa                                                                                                                           <  2:176612/251‑1 (MQ=255)                                                                                                                    cGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCctgctg                                                                                                                >  1:516470/1‑251 (MQ=255)                                                                                                                         aaCGTACGCGAACTGGAAAAAGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGACAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAACCGCACGCACCTGCTGTCACg                                                                                                           >  2:463657/1‑251 (MQ=255)                                                                                                                                 cgAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGc                                                                                                   <  1:628954/251‑1 (MQ=255)                                                                                                                                   aaCTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGGCTGGGGCTGAAACGCACGACCCGGCTGTCACGGATGAAGCgg                                                                                                 >  2:236839/1‑251 (MQ=255)                                                                                                                                    aCTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGc                                                                                                <  1:696766/251‑1 (MQ=255)                                                                                                                                      tGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTg                                                                                              >  1:541609/1‑251 (MQ=255)                                                                                                                                        aaagacgtctatgagccctcgGTATTGCTAACACTCGGTAGCGGGCGGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCTCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCATCGTCTGGGGCGGAAACGCACGACCCTGCTGACACGTATGAAGCGGCTggg                                                                                            <  2:75890/232‑1 (MQ=255)                                                                                                                                          aaaCGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGaa                                                                                          <  2:574454/251‑1 (MQ=255)                                                                                                                                            aCGTCATTGAGCTCGCGGTATTGCTAACACGCGTTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGGGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACTCACGACCCTGCTGTCACGGATGAAGCGTCTGGGAAtt                                                                                        <  2:305035/251‑1 (MQ=255)                                                                                                                                                   tGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATaaa                                                                                  <  1:416423/250‑1 (MQ=255)                                                                                                                                                    gAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATc                                                                                <  1:654274/251‑1 (MQ=255)                                                                                                                                                    gAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATc                                                                                >  1:372019/1‑251 (MQ=255)                                                                                                                                                      gcgcgcGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCgg                                                                              <  1:249244/251‑1 (MQ=255)                                                                                                                                                        gcgcgGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCa                                                                            <  2:523506/251‑1 (MQ=255)                                                                                                                                                          gcgGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCAtt                                                                          <  1:11294/251‑1 (MQ=255)                                                                                                                                                           cgGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTg                                                                         >  1:54327/1‑251 (MQ=255)                                                                                                                                                           cgGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTg                                                                         <  2:229698/251‑1 (MQ=255)                                                                                                                                                              tATTGCTAACACGCGGTACCGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGAtt                                                                      >  1:582096/1‑251 (MQ=255)                                                                                                                                                                           cgGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACATGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAAttt                                                         >  1:216408/1‑250 (MQ=255)                                                                                                                                                                                aCGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCgg                                                     >  2:618090/1‑250 (MQ=255)                                                                                                                                                                                    gctgcAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAg                                                 <  1:523768/250‑1 (MQ=255)                                                                                                                                                                                     ctgcAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTgaaaga                                                                                                                                                                                      >  2:298741/1‑116 (MQ=255)                                                                                                                                                                                     ctgcAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTgaaaga                                                                                                                                                                                      <  1:298741/116‑1 (MQ=255)                                                                                                                                                                                       gcAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCt                                             >  2:611523/1‑251 (MQ=255)                                                                                                                                                                                        cAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAA‑GGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGc                                           >  1:262620/1‑251 (MQ=255)                                                                                                                                                                                         aGCTGTCATTGCCAGATATTGTTTTACCGGACCCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGGTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGc                                           <  2:19311/251‑1 (MQ=255)                                                                                                                                                                                              tCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTc                                      >  2:453806/1‑251 (MQ=255)                                                                                                                                                                                                    ccAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCAt                                <  2:438184/251‑1 (MQ=255)                                                                                                                                                                                                         tatTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGCAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGCCCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACCGATCTGCCTGTCCGGCCTACTAt                           >  2:631612/1‑251 (MQ=255)                                                                                                                                                                                                                  aCCGGAACCTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGt                  >  1:578124/1‑251 (MQ=255)                                                                                                                                                                                                                    cGGAACCTGAACCGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGAGTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCAGGAGGttt                <  2:112317/251‑1 (MQ=255)                                                                                                                                                                                                                         ccTGAAACGCCGCCTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCg           <  1:618090/251‑1 (MQ=255)                                                                                                                                                                                                                                cgccgcCTGCAGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGGTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCCCGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCCTGAGGTTTTTTCGGACGat     >  2:682290/1‑250 (MQ=255)                                                                                                                                                                                                                                  ccgccTGCCGCAGCGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATAtt  >  1:649425/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               GAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCGCACCTTGAGCAACATGGAGTGGCCGGGTAACGTACGCGAACTGGAAAACGTCATTGAGCGCGCGGTATTGCTAACACGCGGTAACGTGCTGCAGCTGTCATTGCCAGATATTGTTTTACCGGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCTGGAGGGCGAAGATGAATATCAGTTGATTGTGCGCGTGCTGAAAGAAACCAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGCGCAACGTCTGGGGCTGAAACGCACGACCCTGCTGTCACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATT  >  NC_000913/2856006‑2856481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑