Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,134,498	G→A	V385V (GTC→GTT)	wzc ←	protein‑tyrosine kinase Wzc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,134,498	0	G	A	100.0%	259.4 / NA	75	V385V (GTC→GTT)	wzc	protein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/32);  total (43/32)

AGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCC  >  NC_000913/2134254‑2134741                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    aGACGCTAATACCGTGGTCTTCCAGTACTTGCGGGCTTTCAAAGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCGCAGCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACctgc                                                                                                                                                                                                                                               <  1:83984/251‑1 (MQ=255)       tAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGAcc                                                                                                                                                                                                                                          >  2:527954/1‑250 (MQ=255)         aTACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGTGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGa                                                                                                                                                                                                                                       <  2:619318/251‑1 (MQ=255)              gtgtTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGCGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCg                                                                                                                                                                                                                                   >  2:411156/1‑250 (MQ=255)                  tcttcCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATc                                                                                                                                                                                                                              >  1:630330/1‑251 (MQ=255)                    ttcCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAACGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATcgc                                                                                                                                                                                                                            >  1:306418/1‑251 (MQ=255)                     tcCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATcgcg                                                                                                                                                                                                                           >  2:262760/1‑251 (MQ=255)                       cAGTACTTGCGGGCTTTCAAGGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCggg                                                                                                                                                                                                                         <  2:166078/251‑1 (MQ=255)                        aGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGt                                                                                                                                                                                                                        <  2:128165/251‑1 (MQ=255)                         gTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTc                                                                                                                                                                                                                       >  1:116896/1‑251 (MQ=255)                           aCTTGCGGGCTTTCAATGCCGCGATTAACCAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGTTGATGTTCAGCTCCTGCTCTTTATTCAGCAGGTGCATATAAACCTGCTGACCAGACTGGACATCGCGGGTCAg                                                                                                                                                                                                                     <  2:609366/251‑1 (MQ=255)                                      ttCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAAtc                                                                                                                                                                                                           <  2:488099/250‑1 (MQ=255)                                                 gATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGtt                                                                                                                                                                                               <  1:527954/251‑1 (MQ=255)                                                     aaaCAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCg                                                                                                                                                                                           <  2:225992/251‑1 (MQ=255)                                                     aaaCAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCg                                                                                                                                                                                           >  1:329457/1‑251 (MQ=255)                                                       acaacGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGc                                                                                                                                                                                         >  2:366429/1‑251 (MQ=255)                                                         aacGAGCGCATCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAAGCGCGCCGAGGATAATCAGCCCGTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCCGCAGTTGCATATAAACCTGCTGACCAGTCTCGACACCGCGGGTCAGACTGACAATCTCCTGCTGGGTTTTCGGCAt                                                                                                                                                                                       <  1:607562/251‑1 (MQ=255)                                                           ccaGCGCAGCAGCACCCCCACGATAGAAAGCATCAGGCCAAGGAGAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCGCTTTATTCAGCAGTTGCATATAAACCGGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTGCGGCATCg                                                                                                                                                                                     <  2:651566/249‑1 (MQ=255)                                                                gcagcaacaCACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTAGGCATCGCCGt                                                                                                                                                                                 >  1:578474/1‑250 (MQ=255)                                                                        acacCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGAcc                                                                                                                                                                        <  1:546821/251‑1 (MQ=255)                                                                         cacCCACGATAGCAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGAGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCCGTTGCATATAAACCTGATGACCAGAATCGACATCGCGGGTCAGACGGACACTCTCCTGATGGGTTTTCGGCATCGCCGTTACGCGACCg                                                                                                                                                                       >  2:436327/1‑251 (MQ=255)                                                                              aCGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAg                                                                                                                                                                  <  2:116896/251‑1 (MQ=255)                                                                                   aGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTc                                                                                                                                                                                                                                    <  2:607952/180‑1 (MQ=255)                                                                                   aGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTc                                                                                                                                                                                                                                    >  1:607952/1‑180 (MQ=255)                                                                                      aaGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGCCGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACACTCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCtt                                                                                                                                                          >  2:534280/1‑251 (MQ=255)                                                                                      aaGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTACGTTTGTCtt                                                                                                                                                          >  2:240805/1‑251 (MQ=255)                                                                                       aGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCGTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCttt                                                                                                                                                         <  2:125535/251‑1 (MQ=255)                                                                                               gCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCtt                                                                                                                                                 >  2:133975/1‑251 (MQ=255)                                                                                                ccAAGGATAATCGCGCCGCGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGAGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCa                                                                                                                                                                                                                      >  1:490367/1‑181 (MQ=255)                                                                                                ccAAGGATAATCGCGCCGATGATAATCAGCCCTTTCTGCGGTTGCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTGTCAGCTCCTGCTCGTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACGCGACATCGCGGGTCa                                                                                                                                                                                                                      <  2:490367/181‑1 (MQ=255)                                                                                                          tCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGGCGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTgc                                                                                                                                      >  2:1585/1‑250 (MQ=255)                                                                                                           cgcgCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCGTCTGACCAGACTCTACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGAc                                                                                                                                     <  2:543425/251‑1 (MQ=255)                                                                                                             cgcCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACCTCGCCGACGGTGCTGGCCTCGGTGATTTTCCGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATAGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGACTGACGt                                                                                                                                   >  2:625035/1‑251 (MQ=255)                                                                                                             cgcCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGt                                                                                                                                   <  1:139092/251‑1 (MQ=255)                                                                                                                   ggATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTcc                                                                                                                             >  2:215006/1‑251 (MQ=255)                                                                                                                      tAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCcagc                                                                                                                          >  1:579869/1‑251 (MQ=255)                                                                                                                        aTCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTTCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCTACATCGCGGGTCAGACTGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCcagcag                                                                                                                        <  1:412898/251‑1 (MQ=255)                                                                                                                                tttCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCggg                                                                                                                >  2:361085/1‑250 (MQ=255)                                                                                                                                    ttcGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGCCGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTACGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGc                                                                                                            >  2:137484/1‑251 (MQ=255)                                                                                                                                       ggTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAAGGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGCCAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTTTCCAGCAGAGTGCGGTACGCCgg                                                                                                         >  2:119505/1‑251 (MQ=255)                                                                                                                                             aGCACGCCAGGATGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGCGCTGGCCTCGGTGATTTTCCGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCCGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTCCGCCgggggacc                                                                                                   >  2:25436/1‑246 (MQ=255)                                                                                                                                                     aGGCTCAGTGATTCCCGGGTCCACAATACGCCCCTCGCCGACGGTGCCCGCCTCGCTGACCTTAAGCTCCTGCTCTTTATTCAGCCGTTGCACATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGCCGGACAATCTCCTGCTGGGTTTTCGGCATCCACGTTACGCGACCGTTCAGTTTGCCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGGGAACTTTGgtgt                                                                                           >  2:716985/1‑251 (MQ=255)                                                                                                                                                        atGAGTGATTGCCGGGTCCACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGGTACGCGACCGTTAAGATGGGCTTTTTCGTCTTCCAGCGCCTGACGTGTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTAc                                                                                         >  1:178316/2‑250 (MQ=255)                                                                                                                                                          gAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACa                                                                                        <  1:393039/249‑1 (MQ=255)                                                                                                                                                          gAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGc                                                                                      >  1:557581/1‑251 (MQ=255)                                                                                                                                                            gTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCtt                                                                                    >  2:542580/1‑251 (MQ=255)                                                                                                                                                               aTTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCg                                                                                                          >  1:304232/1‑226 (MQ=255)                                                                                                                                                               aTTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCg                                                                                                          <  2:304232/226‑1 (MQ=255)                                                                                                                                                                ttGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTgg                                                                                  >  2:734146/1‑249 (MQ=255)                                                                                                                                                                     gggTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTc                                                                           >  1:521632/1‑251 (MQ=255)                                                                                                                                                                          aacaaTGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTa                                                                                          >  1:711847/1‑231 (MQ=255)                                                                                                                                                                          aacaaTGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTAGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTGCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCGCCAGCAGCGGGCGGTACGCCGGGTGAACTTTGGTGTa                                                                                          <  2:711847/231‑1 (MQ=255)                                                                                                                                                                          aacaaTGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCGCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACATCTTGGAGATTTCCGCct                                                                      <  2:306418/251‑1 (MQ=255)                                                                                                                                                                            cactGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCctct                                                                    >  2:414796/4‑251 (MQ=255)                                                                                                                                                                                        cGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGCACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgt                                                        >  1:392658/1‑251 (MQ=255)                                                                                                                                                                                              cGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttcgttc                                                  <  1:132545/251‑1 (MQ=255)                                                                                                                                                                                               ggTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTCAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAgttcgttc                                                  >  2:541503/1‑250 (MQ=255)                                                                                                                                                                                                  gcgggCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTTCATATAAACCTGCTGACCATACTCGACATCGCGTGTCAGACGGACAATCTCCTTCTGTGTTTTCGGCCTCGCCGTTCCGCGACCGTTAAGTTGGGCTTTTTCGGCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAACTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACt                                              <  2:687542/248‑1 (MQ=255)                                                                                                                                                                                                     ggCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTgcg                                           >  1:320053/1‑251 (MQ=255)                                                                                                                                                                                                          cGGTGATTTTCCGCGCCCGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGCCCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGCCCTTTAAGTTTGGCTTTTTCGTTTTCCAGCGCCTGCCGGTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTTGAGATTTCCGCCTCTTTAAAGGTAAGTTCGTTCAACTGCGCATCg                                      <  2:122461/251‑1 (MQ=255)                                                                                                                                                                                                           ggTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCg                                      >  2:28265/1‑250 (MQ=255)                                                                                                                                                                                                            gTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGAt                                    >  2:513133/1‑251 (MQ=255)                                                                                                                                                                                                               aTTTTCAGCCCCTGCTCTTTATTCAGCAGTTGCATATAACCCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGtt                                 <  1:361085/251‑1 (MQ=255)                                                                                                                                                                                                                   tCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCtttt                                                                                                                                                        >  1:134772/1‑128 (MQ=255)                                                                                                                                                                                                                   tCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCtttt                                                                                                                                                        <  2:134772/128‑1 (MQ=255)                                                                                                                                                                                                                    cAGCTCCTGCTCTTTATTCAGCAGTTGCATAGAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCTCCa                            <  2:547559/251‑1 (MQ=255)                                                                                                                                                                                                                     aGCTCCTGCTCTTTAGTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCat                           <  1:25436/251‑1 (MQ=255)                                                                                                                                                                                                                       cTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGGCCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcg                         >  2:247862/1‑251 (MQ=255)                                                                                                                                                                                                                         ccTGCTTTTTATGCAGCAGTTGCATAGAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcg                         <  2:630330/249‑1 (MQ=255)                                                                                                                                                                                                                            gCTCTTTATTCAGCAGGTGCATATAAACCTGCTGACCAGGCTCGACATCGCTGGTGAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGACTTTTGCGTCTTCCAGCTCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTTTACAGCTTGGAGATTTCCGCCTCTTTAAGGGTCAGTTCGTTCAACTGCGCATCGAGGTTCACCatcgaatcg                    <  1:280035/251‑1 (MQ=255)                                                                                                                                                                                                                                  tATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCAcc              >  2:130906/1‑251 (MQ=255)                                                                                                                                                                                                                                  tATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCAcc              >  2:733831/1‑251 (MQ=255)                                                                                                                                                                                                                                      cagcagTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCt           <  1:366429/250‑1 (MQ=255)                                                                                                                                                                                                                                       agcagTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCttt         >  1:222993/1‑251 (MQ=255)                                                                                                                                                                                                                                               cATATAAACCTGCTGACCAGAATCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCCGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTcc  >  1:477921/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    AGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCC  >  NC_000913/2134254‑2134741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑