Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,103,891	G→A	P207S (CCA→TCA)	wbbK ←	putative glycosyltransferase WbbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,103,891	0	G	A	100.0%	52.2 / NA	17	P207S (CCA→TCA)	wbbK	putative glycosyltransferase WbbK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/10);  total (7/10)

AACCTAGAGGGAAAACAAACTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCAT  >  NC_000913/2103662‑2104137                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       aaCCTAGAGGGAAAACAAACTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATAtt                                                                                                                                                                                                                                     <  2:211605/249‑1 (MQ=255)                                      aaCAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGGTATTCTTAAATTGAGAatcatc                                                                                                                                                                                               <  1:270245/250‑1 (MQ=255)                                       aCAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAatcatcat                                                                                                                                                                                             <  1:491871/251‑1 (MQ=255)                                          aTGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAatcatcatc                                                                                                                                                                                            >  2:62913/1‑249 (MQ=255)                                               cGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGt                                                                                                                                                                                     <  1:231888/251‑1 (MQ=255)                                                                       cGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATGTATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCAGCAGTAAGTTGGCTTTTATCAGAtaatttaatt                                                                                                                                                             <  1:597180/251‑1 (MQ=255)                                                                                                     cAAGACTGATAATATATTTTGCATCCGCATTTTCTGTACCAATGATAGTAAGCAGCAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGttatttat                                                                                                                                >  2:714788/1‑250 (MQ=255)                                                                                                                atatatTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCtt                                                                                                                    <  2:108902/251‑1 (MQ=255)                                                                                                                         gCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAAtt                                                                                                            <  2:47907/250‑1 (MQ=255)                                                                                                                                 aTTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATACTAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGaa                                                                                                                                                                                                                         >  1:113318/1‑133 (MQ=255)                                                                                                                                 aTTTTCTGTACCACTGATAGTAAGCAGAAAGTTAATATTTGATTGTTCTTTCAATTTCCTTGCTGCACTAATAAGAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCATCTCAGaa                                                                                                                                                                                                                         <  2:113318/133‑1 (MQ=255)                                                                                                                                                                         gATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAAt                                                           >  2:19128/1‑251 (MQ=255)                                                                                                                                                                                                 gctcTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGATAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGta                                   <  1:700253/248‑1 (MQ=255)                                                                                                                                                                                                  cACTAATAATAAGCTCGTGATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTAAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGtat                                  >  1:190729/1‑251 (MQ=255)                                                                                                                                                                                                  cACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGtat                                  >  1:354349/1‑251 (MQ=255)                                                                                                                                                                                                                tCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATa                     >  2:468109/1‑250 (MQ=255)                                                                                                                                                                                                                                  cGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTCAAATAAGAAAAAGCTAGGCTCCAt  <  1:62913/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       AACCTAGAGGGAAAACAAACTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCAT  >  NC_000913/2103662‑2104137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑