Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,086,634	G→A	A143A (GCC→GCT)	plaP ←	putrescine:H(+) symporter PlaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,086,634	0	G	A	100.0%	213.6 / NA	62	A143A (GCC→GCT)	plaP	putrescine:H(+) symporter PlaP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/35);  total (27/35)

TCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAGGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTA  >  NC_000913/2086389‑2086871                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              tcCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGCGAAGCACAGGATTGTCGCCCCGGTAATCAACGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGAAGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGt                                                                                                                                                                                                                                          >  2:586586/1‑251 (MQ=255)  ccGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCAACGCCAGACCAGAAGGGACGAGTGCTCGCCAGCGTTCCAGCGCCTTCACCTTCAAATAGGCCAAAAACAACCATGCCCAGAAGCGCCACGATCAGCACGACCTGCAACACGACGATGACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTc                                                                                                                                                                                                                                         <  1:734186/251‑1 (MQ=255)       aGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAAGCATCGGGATAACAGGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCCGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATaaa                                                                                                                                                                                                                                    <  1:4707/251‑1 (MQ=255)            gCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGcc                                                                                                                                                                                                                                <  1:386904/250‑1 (MQ=255)              cgctcgtcccgatctcGGTAAAGGAGAAGCACAGGAGTGTCGCCCCGGTAATCATCGGGATAACATGCCCATCGCCAGCCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGccacc                                                                                                                                                                                                                             <  2:119426/236‑1 (MQ=255)               gATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGccacca                                                                                                                                                                                                                            <  1:590329/251‑1 (MQ=255)               gATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGccacc                                                                                                                                                                                                                             >  2:43785/1‑250 (MQ=255)                        aaaGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGccacca                                                                                                                                                                                                                   <  2:167322/251‑1 (MQ=255)                          aGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACcaca                                                                                                                                                                                                                 <  1:466909/251‑1 (MQ=255)                             cGGTAAAGGCGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTCCGCAGGTTAAAAGCGGTCATAAAGGccacc                                                                                                                                                                                                                             >  1:119426/1‑236 (MQ=255)                                  aaGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCAGCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTGCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCa                                                                                                                                                                                                         <  2:384050/251‑1 (MQ=255)                                      aggaGCACGGGGTTGTCGCCCAGGGAAGCATCGGGAAAAAATGCTCATCTCCATAACAGAATGGGCGAGTGGTCGCCACCGTACTAGCGCCGTCACCTTCAAATACGCCATAAACAGCCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACGCGACGATTAGAGTTTGGAAGTTCGCTACGGATTTCAGACGTGGCAGGTTAAAAGTGGTCATAAAGGCCACTAGCGCCACCACAATCATGCACgat                                                                                                                                                                                                     <  2:330215/248‑1 (MQ=255)                                       gaAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACgatg                                                                                                                                                                                                    >  1:147640/1‑251 (MQ=255)                                       gaAGCACAGGATTGGCGCCCCGGTAATCAGCGGGATAACATGCGCATCGCCATACCAGAAGGGACGAGTGCGCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCTCCACCACAAACAGCCACgatg                                                                                                                                                                                                    <  2:124747/251‑1 (MQ=255)                                        aaGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGAGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCATCGCCCCCACAAACATCCACgaggg                                                                                                                                                                                                   >  2:16085/1‑248 (MQ=255)                                                 aTTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAgg                                                                                                                                                                                          <  1:243031/251‑1 (MQ=255)                                                      cGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCAcc                                                                                                                                                                                      >  1:482084/1‑250 (MQ=255)                                                           cGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCt                                                                                                                                                                                <  1:12367/251‑1 (MQ=255)                                                                  catcGGGATAACATGCGCATCGCCCGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAAt                                                                                                                                                                         >  2:534815/1‑251 (MQ=255)                                                                       ggATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGAtt                                                                                                                                                                    >  2:214429/1‑251 (MQ=255)                                                                       ggATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGAtt                                                                                                                                                                    <  2:619270/251‑1 (MQ=255)                                                                         aTAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATACAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGAtttt                                                                                                                                                                  >  2:606968/1‑251 (MQ=255)                                                                          tAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCAAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTCCAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTc                                                                                                                                                                 >  1:538170/1‑251 (MQ=255)                                                                              aTGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACAAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGCAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAACCATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGcc                                                                                                                                                              >  1:698717/1‑250 (MQ=255)                                                                                    aTCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGaa                                                                                                                                                       <  2:3480/251‑1 (MQ=255)                                                                                        ccagaccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGtt                                                                                                                                                   <  1:647248/251‑1 (MQ=255)                                                                                                gaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCg                                                                                                                                           <  2:640986/251‑1 (MQ=255)                                                                                                    ggACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGgcg                                                                                                                                        <  2:148315/250‑1 (MQ=255)                                                                                                         aGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACa                                                                                                                                  <  1:707597/251‑1 (MQ=255)                                                                                                            gCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGAt                                                                                                                               <  1:553156/251‑1 (MQ=255)                                                                                                                     cgtatccgcgcCTTATCCTTCAAATACTCCATAAACAACCATGCCCAGAATCACCGCGATGAGCACTAACTGCAACACGACGATTACAGTGTTGAAGTTCGGTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGTCCAACAGAGCCGCCACAAACATCCAAGATGGGATGGAAGGCACCAGAGCTTCAACATAGAATCTCGCCAGTAGAATGGTGATCATCGGCGCGAACTGATAGTCGTGCa                                                                                                                      <  2:497979/244‑1 (MQ=255)                                                                                                                        aCCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTCCCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTGGCGCCAGTAGAATGTTGATCATCGGCTCGAACAGATAGTCGAGCaga                                                                                                                    <  2:710877/250‑1 (MQ=255)                                                                                                                           aGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTAACTGCAACACGACGATTCCAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGACAAGATAGACGAGCCgaga                                                                                                                  >  2:114910/1‑249 (MQ=255)                                                                                                                                cttcaccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTAAGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCa                                                                                                            >  1:610140/1‑250 (MQ=255)                                                                                                                                  tcaccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCaa                                                                                                           <  1:193837/249‑1 (MQ=255)                                                                                                                                   caccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAAcc                                                                                                         <  1:13730/250‑1 (MQ=255)                                                                                                                                    accttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTCAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAAccc                                                                                                        >  1:458105/1‑250 (MQ=255)                                                                                                                                               cGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCg                                                                                            <  1:455546/251‑1 (MQ=255)                                                                                                                                                  cATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACa                                                                                         <  1:43785/251‑1 (MQ=255)                                                                                                                                                   aTAAACAACCATGCCCAGAATCACCGCGATCCGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCgcc                                                                                          >  2:92175/1‑247 (MQ=255)                                                                                                                                                        catccATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGt                                                                                                                                                    <  1:679915/183‑1 (MQ=255)                                                                                                                                                        caacCATGCCCAGAATCACCGCGATCAGCACTCCCGGCAACACGACTATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGTATGGAAGTCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCTAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGGCggg                                                                                   <  1:465499/251‑1 (MQ=255)                                                                                                                                                        caacCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACCCGAAGATTACAGTGTTGAAATTCGCTACGGATTTCAGCCTACGCGGGATCAAAGCGGTCATAAAGGCCACCAGCGCCACCACACACATCCACGATGGGATGGACGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGt                                                                                                                                                    >  2:679915/1‑186 (MQ=255)                                                                                                                                                            cATGCCAAGAATCACCGCGCTCAGCACTCCCTGCCACACGACGATTCCCGTGTTGAAGTTCGCTACGGATTTCAGACTACGCCGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCCGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGGCGGGCTa                                                                                >  2:468250/1‑250 (MQ=255)                                                                                                                                                                  cAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGAtt                                                                         >  1:176217/1‑251 (MQ=255)                                                                                                                                                                    gAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCt                                                                                 <  1:3717/241‑1 (MQ=255)                                                                                                                                                                    gAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCt                                                                                 >  2:3717/1‑241 (MQ=255)                                                                                                                                                                      aTCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTg                                                                     <  1:297191/251‑1 (MQ=255)                                                                                                                                                                             cgATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTa                                                               >  2:256566/1‑250 (MQ=255)                                                                                                                                                                               aTCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGTGCGTAAgt                                                            >  1:422614/1‑251 (MQ=255)                                                                                                                                                                                                 acgacgATTACCGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCcagc                                             >  2:47293/1‑248 (MQ=255)                                                                                                                                                                                                 acgacgATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCcagc                                             >  2:204394/1‑248 (MQ=255)                                                                                                                                                                                                          acacgcttgaaatgcGCTACGGATTTCATACTACGCAGGTTAAAAACGGTCATAAAGGCCACCAGCGCCACCACAAACAGCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCGAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAgc                                  <  1:485383/236‑1 (MQ=255)                                                                                                                                                                                                            aGTGTTTAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGACTAGTGGATTTCTGGGCGTAAGTGAATGCAGAGCCAGCAGAAGGATAgcggc                               >  1:192904/1‑251 (MQ=255)                                                                                                                                                                                                             gtgtTGACGTTCGCTCCGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGCTGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTCGAATGTTGATCCTCGGCGCGACCAGATAGTCGAGCAGAGAAGACCAACCCACCATAACGCCGACAGTCGGGCTAATGGATTTCTGGGCGGAAGTGTATGCAGAGCCAGCAGAAGGATAgcggcg                              >  2:643574/1‑251 (MQ=255)                                                                                                                                                                                                               gtTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACAGCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGaa                            <  1:3094/251‑1 (MQ=255)                                                                                                                                                                                                                   aaGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTGTGGGCGTAAGTGTATGCAGAGCCAGCAGTAGGATAGCGGCGAACCAg                        <  1:643574/251‑1 (MQ=255)                                                                                                                                                                                                                        cGCTACGGATTTCAGACTCCGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTcc                   >  2:574880/1‑251 (MQ=255)                                                                                                                                                                                                                         gCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTccc                  <  1:106268/251‑1 (MQ=255)                                                                                                                                                                                                                              ggATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGc             >  1:312766/1‑251 (MQ=255)                                                                                                                                                                                                                                     aGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGAGAGCGGCGAAGCAGCTGCCCGTAGCTCAGAGc      <  2:476319/251‑1 (MQ=255)                                                                                                                                                                                                                                          aCGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTa  <  1:421366/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              TCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAGGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTA  >  NC_000913/2086389‑2086871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑