Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,721,1360CT97.1% 110.8 / ‑5.7 35L38F (CTT→TTT) ydhIDUF1656 domain‑containing protein YdhI
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (16/17);  total (16/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGCTAAAACAAATGTATTGCTGCATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGCTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGG  >  NC_000913/1720893‑1721365
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     
tGCTAAAACAAATGTATTGCTGCATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCg                                                                                                                                                                                                                                 <  2:621698/250‑1 (MQ=255)
      aaCACATGTATTGCTGCATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGACTATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCg                                                                                                                                                                                                                          >  2:363106/1‑251 (MQ=255)
                  gctgcATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGAGCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTgg                                                                                                                                                                                                              <  1:363106/251‑1 (MQ=255)
                              tACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGc                                                                                                                                                                                                  >  2:659220/1‑251 (MQ=255)
                                 cGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCgg                                                                                                                                                                                               <  2:377019/251‑1 (MQ=255)
                                           atTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTAGTAATCTAACGCATTATATGCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTGCGTCTACTTGCCTCCGTCTTTCAAATCATTCGCGTTTGGATTCGTCATCTGGATTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTgg                                                                                                                                                                                     <  2:116900/251‑1 (MQ=255)
                                                      aTTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGtt                                                                                                                                                                          <  2:229448/251‑1 (MQ=255)
                                                      aTTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGACGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGtt                                                                                                                                                                          >  1:64511/1‑251 (MQ=255)
                                                            cgTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGa                                                                                                                                                                    <  1:730783/251‑1 (MQ=255)
                                                                         caattAAAGAATATTTATTAATCTAACGCAATATATTCGGTCTTAAAGGAATCTTCTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGttt                                                                                                                                                       <  2:416234/248‑1 (MQ=255)
                                                                         cACTTAAAGAATATTTATTAATCTAACGCAATATTTTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACGTTCCTCCGTTTTTCAAAGCATTCGCGTTGGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGTTGGATCTACGCCGGTGACATCGGGCATCCCTTGTTAATGGATTTATCGCTGttt                                                                                                                                                       <  2:338407/251‑1 (MQ=255)
                                                                                            aaTCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTTCTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTg                                                                                                                                    <  2:393221/251‑1 (MQ=255)
                                                                                            aaTCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTCCCTTg                                                                                                                                    >  2:140764/1‑251 (MQ=255)
                                                                                                           atTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTAATTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTg                                                                                                                      >  2:268558/1‑250 (MQ=255)
                                                                                                              cGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTg                                                                                                                  >  2:617190/1‑251 (MQ=255)
                                                                                                                        ggAATATACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACt                                                                                                        >  2:424323/1‑251 (MQ=255)
                                                                                                                               aCTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCATTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGCTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCTGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTc                                                                                                   <  1:18937/249‑1 (MQ=255)
                                                                                                                               aCTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCACTTACAAGATCTGGTGTGCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCATGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCGTGCTCTGGCAATACTGATTGCGTGGTAACGATGTc                                                                                                   <  2:474832/249‑1 (MQ=255)
                                                                                                                                        aGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGt                                                                                                                                                                           >  1:494255/1‑168 (MQ=255)
                                                                                                                                        aGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCGTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGt                                                                                                                                                                           <  2:494255/168‑1 (MQ=255)
                                                                                                                                            tATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTa                                                                                     <  1:665580/250‑1 (MQ=255)
                                                                                                                                                  cAATGCTACAGGATTGCCCTTACAAGAGCTGGTTTGCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCAGTTGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGACTTGCTCTGGCAATACTGATGGCGTGGTAACTATGTCAATTAAAACAAGTAAGTAtt                                                                               <  2:627147/250‑1 (MQ=255)
                                                                                                                                                  cAATGCAACATGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCGCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAATCAATTAAGTAt                                                                                <  2:64511/249‑1 (MQ=255)
                                                                                                                                                  cAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTAttt                                                                              >  2:16522/1‑251 (MQ=255)
                                                                                                                                                     tGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTtctc                                                                           >  1:395559/1‑251 (MQ=255)
                                                                                                                                                         aCAGGCTTGCCCTTACACGCTCTGCAGTTCGGAGCACCCGTATACTTTCCTCCGTTTTTCCAAGCATACGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTACCTATGTCCATTCAAACAATTAAGTATTTCTcaac                                                                        >  2:368472/1‑250 (MQ=255)
                                                                                                                                                         aCAGGATTGCCCCAACAAAACCTGGAGTTCGGAGCGTCAGTCTACTTTCCTCTGTTCTTCAAAGCATTCGCGCTCGCATTCGTCATCTGGTTTGTCCTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGAAACTATGTCACTTAAAACAATTAAGTCTTTCTcaac                                                                        >  2:335932/1‑250 (MQ=255)
                                                                                                                                                              agttcccTTACAAGATCTGGTGTTCGGGGCGCCCGTCTACGTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCAt                                                                  <  2:478328/247‑1 (MQ=255)
                                                                                                                                                                tGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTg                                                                >  1:427613/1‑251 (MQ=255)
                                                                                                                                                                                                  cTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTAt                                                                                                      >  1:260615/1‑179 (MQ=255)
                                                                                                                                                                                                  cTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTAt                                                                                                      <  2:260615/179‑1 (MQ=255)
                                                                                                                                                                                                      tttCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGGCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGGGACGTCTGGCATCCCTTGTGTATGGATTTAGCGCGGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTGACTATGTCAATTAAAACaa                                                                                        <  2:669434/189‑1 (MQ=255)
                                                                                                                                                                                                      tttCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTCTGCCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACaa                                                                                        >  1:669434/1‑189 (MQ=255)
                                                                                                                                                                                                                   tCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATg              <  1:291873/250‑1 (MQ=255)
                                                                                                                                                                                                                               cgTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTATGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTgg  >  1:678187/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     
TGCTAAAACAAATGTATTGCTGCATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGCTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGG  >  NC_000913/1720893‑1721365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑