Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	75.6 / NA	21	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/11);  total (10/11)

TTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGC  >  NC_000913/1652102‑1652570                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                ttATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGcagaaca                                                                                                                                                                                                                             <  2:51309/250‑1 (MQ=255)                                      ggATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCGATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAg                                                                                                                                                                                        <  1:86778/250‑1 (MQ=255)                                                aCATAGATCTGAAAAATGGAACAATGCTGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTTATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACCTTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAAAAAAATGCACCTTTGTTTTTCAACCTGAAGGGTCAGCgagg                                                                                                                                                                             >  2:695274/1‑250 (MQ=255)                                                 cATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCGCCTTTGTTTTTCAACCTGAAGTGTCAGCGagag                                                                                                                                                                            <  1:609939/251‑1 (MQ=255)                                                    aGATCTGAAAAATGGAACAATGATGATCAGGAGCAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTCATTCATCTTATTAAGCCCGCAAACGACGCTCCCCCGAGTCAGATGACATTAACCGGACTGAGCCAAGAGCATATCATTGATGTTCACCTCCGAGAGTATGGCAGAACAGAAAAACAAAAATGCACATTTGTTTTTTACACTGAAGTGTCCGAGAGAGTa                                                                                                                                                                          >  2:146905/1‑250 (MQ=255)                                                                   aaCAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTTAGGCAGCAATCGACGCTCTCCGGAGTCCGGTGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGGATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGGAGTGTCAGCGAGAGTAAAAAATTATGGTGAcc                                                                                                                                                          >  1:65308/1‑251 (MQ=255)                                                                                            gCAAAAGACCGTGTCACGGTACCAAAAACACAGGCGGGAACCAGTCGGGGCATTCATCTTATTAAGCCATCAATCGACGCTCTCCGGAGTCAGATGACAGTAACGAGACGGAGCAAAGAGCATATCATTGATGTTCACCTCAGATAGTATGGCAGAACAGAAAAACAAAAAGGCACCTTATTTTTGCAACCGTAAGTGTCAGCGAGAGTAAAAAATTATGTTTGCCATTTTGCCGTTGACTCAATAAGGCa                                                                                                                                 <  2:668456/251‑1 (MQ=255)                                                                                             cAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCCATCGGGTAATTCATCTTATTAAGCCCGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTTATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATTCACCTTTGTTTTTCAAACTTAAGTGTCAGCGAGAGGAAAAAATTATGGTGACCAATTTACCGTTGACTCAATAAGGCa                                                                                                                                 >  1:241324/1‑250 (MQ=255)                                                                                                              gTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGAt                                                                                                               <  2:236116/251‑1 (MQ=255)                                                                                                                           aGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCCATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCgg                                                                                                   >  2:294009/1‑250 (MQ=255)                                                                                                                            ggCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGCTGTGGGACGCAGCGATAAAACGTGCCGGAc                                                                                                 >  2:619067/1‑251 (MQ=255)                                                                                                                               gggAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTcc                                                                                              <  1:294009/251‑1 (MQ=255)                                                                                                                               gggAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTcc                                                                                              >  2:175745/1‑251 (MQ=255)                                                                                                                                  aaCCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCGGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATGTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGcc                                                                                           <  2:30338/251‑1 (MQ=255)                                                                                                                                                             gCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATa                                                                <  1:8790/251‑1 (MQ=255)                                                                                                                                                              ccAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCAGTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAAGCATATCAGTCGAGACATAc                                                               <  1:156848/251‑1 (MQ=255)                                                                                                                                                                                         aTGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTgg                                    >  1:11792/1‑251 (MQ=255)                                                                                                                                                                                                    gagaCTGAGCAAAGAGAATCTCAATGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAAAAAAAATACACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTAGGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGAACGCAGAGATAAAACGTGCCGGACTACGCCATCGAAAATCATATAAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCAAACCCgg                         >  2:619416/1‑251 (MQ=255)                                                                                                                                                                                                      gaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATc                                                                                        <  1:260817/186‑1 (MQ=255)                                                                                                                                                                                                      gaCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATc                                                                                        >  2:260817/1‑186 (MQ=255)                                                                                                                                                                                                                            tGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGc  <  2:11792/250‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                TTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGC  >  NC_000913/1652102‑1652570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑