Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,527,251	C→T	intergenic (+99/‑2671)	yncH → / → ydcD	DUF5445 domain‑containing protein YncH/uncharacterized protein YdcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,527,251	0	C	T	100.0%	84.2 / NA	25	intergenic (+99/‑2671)	yncH/ydcD	DUF5445 domain‑containing protein YncH/uncharacterized protein YdcD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

AAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTT  >  NC_000913/1527005‑1527487                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             aaaGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATACTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAACAATTGAGTAATAAATATGt                                                                                                                                                                                                                                          <  1:539596/251‑1 (MQ=255)   aGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTAGTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTg                                                                                                                                                                                                                                        <  2:369601/251‑1 (MQ=255)     aaaCTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGAt                                                                                                                                                                                                                                      <  2:311965/251‑1 (MQ=255)       actaactaTTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGAttt                                                                                                                                                                                                                                    <  2:704698/251‑1 (MQ=255)       actaactaTTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGAttt                                                                                                                                                                                                                                    >  1:537451/1‑251 (MQ=255)              aTTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCa                                                                                                                                                                                                                               <  2:477791/249‑1 (MQ=255)                             atttttCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTAttt                                                                                                                                                                                                              <  1:6608/248‑1 (MQ=255)                                    aGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGCGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGt                                                                                                                                                                                                       >  2:517965/1‑251 (MQ=255)                                                              ctcAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAAt                                                                                                                                                                              >  1:596641/1‑250 (MQ=255)                                                                      gTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCa                                                                                                                                                                     <  1:477347/251‑1 (MQ=255)                                                                                    atatGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGAGTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTacac                                                                                                                                                       <  1:466789/251‑1 (MQ=255)                                                                                                                     ataCAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTg                                                                                                                      >  1:707686/1‑251 (MQ=255)                                                                                                                                    tCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATc                                                                                                         >  2:310284/1‑249 (MQ=255)                                                                                                                                         cAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTAct                                                                                                  >  2:713721/1‑251 (MQ=255)                                                                                                                                                      aGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCGAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAg                                                                                     <  2:596641/251‑1 (MQ=255)                                                                                                                                                            aaTGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCa                                                                                   >  1:167698/1‑247 (MQ=255)                                                                                                                                                                          tatttattCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAGATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAgg                                                                 >  2:250155/1‑251 (MQ=255)                                                                                                                                                                               attCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAAAACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGCAGCAAGataatacgcacccaggtgggg                                                            >  1:102899/1‑232 (MQ=255)                                                                                                                                                                                       ttATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGa                                                                                       <  2:578670/216‑1 (MQ=255)                                                                                                                                                                                       ttATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGa                                                                                       >  1:578670/1‑216 (MQ=255)                                                                                                                                                                                            tGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTg                                               >  2:81194/1‑251 (MQ=255)                                                                                                                                                                                                           gagaGCTGGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACTCATTAGTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCAGCTGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTct                                <  2:472133/251‑2 (MQ=255)                                                                                                                                                                                                             gagCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCggg                              <  1:195395/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaaCAGCAATTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTAt         <  2:537451/251‑1 (MQ=255)                                                                                                                                                                                                                                         aaTTGATTAATAAATATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGGCCCCTCGGGGGAGTTGTCATTATTGTCTATGCTAttt  <  2:707686/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             AAAGAAACTAACTATTCACATCGTGCAGATGTTTCAGTTGTTATCACAGGCATTCTATAATCTCAAAATGTTTTTAATGATGGATATGCTCGGAGTTGGAGATGCAATTAATATTAATACAAATAAAAATATCCGGCAGGTATGCTAAAAGGATAAATGGCGTTCTCTGTATTTATTCGCAATTATCTGTAGGACAAATATGGAGAGCTTGATGAGTCAGAGCTAAAACAGCAATTGATTAATAAACATGTTGATTTATTCAAGAAAATGGATGTATTTTAAATGTTGATTCTTAATATAACCTAATCAATAGATTATCAAGTGAGAGCTACACATTATTGGAGGGGCAGGGGAAAACACCATTTTGGGAACAATGCATCAGGTACTCTTACCACTGAAGCAAGATCATCCGGCACCAGGTGGTGGTGCAGTGCTTTGGAAATGGTCCCCTCGGGGGAGTTGTCATTATTTTCTATGCTATTT  >  NC_000913/1527005‑1527487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑