Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,453,992	G→A	W22* (TGG→TGA)	paaA →	phenylacetyl‑CoA 1,2‑epoxidase, monooxygenase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,453,992	0	G	A	100.0%	161.5 / NA	47	W22* (TGG→TGA)	paaA	phenylacetyl‑CoA 1,2‑epoxidase, monooxygenase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/25);  total (22/25)

CACTGATAAATAATGATTTATAAAAATAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGGATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGAT  >  NC_000913/1453768‑1454231                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                cACTGATAAATAATGATTTATAAAAATAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACAtt                                                                                                                                                                                                                       <  2:587313/251‑1 (MQ=255)    tGATAACTAATGATTTATAAAACTAGGGTGCGAAATCCGTCCCAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGAATGAATGCCCGATGCTTACCGAAAGACATTGAt                                                                                                                                                                                                                    >  2:341480/1‑251 (MQ=255)          ataatGATTTTTAAAAATAGGGTGCGAAATCCGTCACCGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCCACGGATAGCCCAGGAAACGGCCATCGAGCCACAGGACTGAATGCCAGATGCTTACCGACAGACATTGATCCGcc                                                                                                                                                                                                               >  2:620765/1‑250 (MQ=255)           taatGATTTATAAAAATAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTGACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGcc                                                                                                                                                                                                               >  2:189245/1‑249 (MQ=255)                aTTTATAAAAATAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGAGCCGCCAGATTgg                                                                                                                                                                                                        <  2:387236/251‑1 (MQ=255)                 tttATACAAATAGGGTGCGAAATCCGTCTCAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACACTGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGCAAGACATTGATCCACCAGATTggg                                                                                                                                                                                                       >  2:520252/1‑251 (MQ=255)                      aaaaaTAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTTATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGGGAGGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGgcagc                                                                                                                                                                                                   <  2:312478/250‑1 (MQ=255)                      aaaaaTAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTACTTAATTCACGAACACTGGATTCGTACAGGAGATGACGTGACCCACGAAGCACGCTTTGAGCAACGGATAGCCAAGGAAACGACTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGTCATTGATCAGCCAGATTGGgcagct                                                                                                                                                                                                  >  1:478465/1‑250 (MQ=255)                      aaaaaTAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGgcagca                                                                                                                                                                                                  <  2:632423/251‑1 (MQ=255)                       aaaaTAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATg                                                                                                                                                                                                                                              <  2:649167/206‑1 (MQ=255)                       aaaaTAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATg                                                                                                                                                                                                                                              >  1:649167/1‑206 (MQ=255)                                     aaTCCGTCACAGTTCAAACATACAAAATTTGTGATTTTGCTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCg                                                                                                                                                                                       >  2:141838/1‑247 (MQ=255)                                     aaTCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCg                                                                                                                                                                                       >  2:238185/1‑247 (MQ=255)                                      aTCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTAGCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCTCCATATTGGGCAGCATGCGCACTCCGAGAtt                                                                                                                                                                                  <  2:367171/251‑1 (MQ=255)                                       tCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCAAGCAAACTGGAATCGTCAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGACACGGCTATCGAGCCACAGGACTGAATGCCAGATGCTTACCGAAAGACATTGCTCCGCCAGATTGGGCAGCATGCGCACTCCGCGAttg                                                                                                                                                                                 >  1:99717/1‑251 (MQ=255)                                                     aaCATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCt                                                                                                                                                                   <  1:102051/251‑1 (MQ=255)                                                          acaaaagatgtgATTTTACTTAACTATTGTGTAGCTTTCATAAAACAATGTGATTCGTGTTGTTAATAAATTCACGAAAACTGGAAGCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTAACGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGAGTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAgg                                                                                                                                                              <  1:362087/243‑1 (MQ=255)                                                                    tgATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTTAAGGTAACTGGATc                                                                                                                                                    >  1:123173/1‑251 (MQ=255)                                                                              tAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGCTAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCAc                                                                                                                                          >  1:33179/1‑251 (MQ=255)                                                                                              ttCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGt                                                                                                                            <  1:583512/249‑1 (MQ=255)                                                                                                cATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTaa                                                                                                                          >  2:469851/1‑249 (MQ=255)                                                                                                   aaaaCAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGcc                                                                                                                      <  2:33179/250‑1 (MQ=255)                                                                                                    aaaCAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCAt                                                                                                                    >  1:518661/1‑251 (MQ=255)                                                                                                                  gtgtTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGATGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAg                                                                                                      <  2:559798/251‑1 (MQ=255)                                                                                                                        ttaattaaTTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAgg                                                                                                >  1:725999/1‑251 (MQ=255)                                                                                                                            ttaattCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATg                                                                                             >  1:587288/1‑250 (MQ=255)                                                                                                                              aattCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGaa                                                                                           <  1:469851/250‑1 (MQ=255)                                                                                                                              aattCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACGGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGaa                                                                                           <  2:123173/250‑1 (MQ=255)                                                                                                                                            ggAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTg                                                                            <  1:263534/251‑1 (MQ=255)                                                                                                                                               aTCGTAAAGGGGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTAt                                                                         >  1:672510/1‑251 (MQ=255)                                                                                                                                                 cGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTAtct                                                                       <  1:580524/251‑1 (MQ=255)                                                                                                                                                  gTAAAGGCGCTGACGTGACCCAAGAAGAAAGCTTTGAGCAACGGATAGCCCAGGACACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGCGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGACGCCGGTCATGGTCTGTAtctc                                                                      >  2:476820/1‑251 (MQ=255)                                                                                                                                                            tgaCGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACCGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGcgccg                                                            >  1:291917/1‑251 (MQ=255)                                                                                                                                                               cGTGACCAAAGAAGAACGCTTTGAGCACCGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGccgccg                                                         <  2:4459/251‑1 (MQ=255)                                                                                                                                                                gTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGa                                                        <  1:29640/251‑1 (MQ=255)                                                                                                                                                                        agaagaACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTgg                                                >  1:210860/1‑251 (MQ=255)                                                                                                                                                                                         aaCGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGAc                               >  2:401246/1‑251 (MQ=255)                                                                                                                                                                                          aCGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGagaaga                                >  1:558405/1‑249 (MQ=255)                                                                                                                                                                                          aCGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGagaaga                                <  2:558405/249‑1 (MQ=255)                                                                                                                                                                                            ggATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATc                            <  1:476820/251‑1 (MQ=255)                                                                                                                                                                                             gagaGCCCAGGAAACGGCGATCGAGCCACAGGACTGAAGGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGAAAGCATGAGCACTCCGAGATGGGTTGTAGGTTGCCTAAAGGTAACTGGATCACGCGCGCACCAACCTTGCGGCGGAAAGCCAGTCTGTTGGCCAAAGTCCAGGAAGAAGCCGGTCATGGTCTGTAGCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCt                           <  1:715288/248‑1 (MQ=255)                                                                                                                                                                                                aGCCCAGGAAACGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTAc                         <  1:238185/250‑1 (MQ=255)                                                                                                                                                                                                      ggAAACGTGTATCGGGCCACAGGACTTAAGGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGGTGGTATGTTGCCTGAAGGTAACAGGATCACTCGCGCACCAACCTTGCGGCGTAGATCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATGTCTATAGCAACGCCGAAACACTGGGCTGCGGCAGAGAAGACATCTACCaaaaaa                  <  2:99717/251‑1 (MQ=255)                                                                                                                                                                                                           cGGCTATCGAGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTc             <  1:141838/251‑1 (MQ=255)                                                                                                                                                                                                                    aGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGacggacgg    <  1:341480/251‑1 (MQ=255)                                                                                                                                                                                                                    aGCCACAGGACTGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGCGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGacggacgg    >  1:671618/1‑251 (MQ=255)                                                                                                                                                                                                                      ccACAGGACGGAATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGAACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGGCATGGTCTGTATCACTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGAt  <  1:620765/251‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                CACTGATAAATAATGATTTATAAAAATAGGGTGCGAAATCCGTCACAGTTCAAACATACAAAATTTGTGATTTTACTTAACTATTGTGTAACTTTCATAAAACAATGTGATTCGTGTTTTTAATTAATTCACGAAAACTGGAATCGTAAAGGTGATGACGTGACCCAAGAAGAACGCTTTGAGCAACGGATAGCCCAGGAAACGGCTATCGAGCCACAGGACTGGATGCCCGATGCTTACCGAAAGACATTGATCCGCCAGATTGGGCAGCATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGAT  >  NC_000913/1453768‑1454231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑