Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	146,734	IS30 (+) +2 bp	intergenic (‑40/‑233)	panD ← / → rpnC	aspartate 1‑decarboxylase proenzyme/recombination‑promoting nuclease RpnC

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	146734 =	0 (0.000)	70 (0.970)	66/492	0.2	100%	intergenic (‑40/‑234)	panD/rpnC	aspartate 1‑decarboxylase proenzyme/recombination‑promoting nuclease RpnC
	?	NC_000913	= 1470516	NA (NA)					noncoding (1221/1221 nt)	IS30	repeat region
*	?	NC_000913	= 146735	0 (0.000)	59 (0.810)	52/492	0.4	100%	intergenic (‑41/‑233)	panD/rpnC	aspartate 1‑decarboxylase proenzyme/recombination‑promoting nuclease RpnC
	?	NC_000913	270541 =	NA (NA)					noncoding (1/1221 nt)	IS30	repeat region

GGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACAC  >  NC_000913/270535‑270783       |                                                                                                                                                                                                                                                   ggCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGacac  <  1:398629/249‑1 (MQ=31)       |                                                                                                                                                                                                                                                   GGCTACTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGCGGGCTGTAGCTCACCTGACAC  >  NC_000913/270535‑270783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.