Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,111,7270CT100.0% 85.4 / NA 29P289M (CCG→TTG) opgHosmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/14);  total (15/14)
*NC_0009131,111,7280CT100.0% 92.0 / NA 29P289M (CCG→TTG) opgHosmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/14);  total (15/14)

CTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGC  >  NC_000913/1111517‑1111956
                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                    
cTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCt                                                                                                                                                                                               >  1:4908/1‑251 (MQ=255)
     tATTGGTCGCGATAAATACAGTATATCTGCGGCAACAGTTGGCGATGAACCATTAAACCCGGAGGATCGCACGGCGTTGATCATGCCTATCTGTAACTAAGACGTGAACCGTGTTTTTGCTGGCCTGCTTGCAACGTTGGAATCAGTAAAAGCCACCGGGAATGCCAAACACCGTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTTGCAGAGCAAAAAGGCTAGATGGAGCTTag                                                                                                                                                                                            <  2:338878/249‑2 (MQ=255)
                         gTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCGACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGt                                                                                                                                                                      <  2:324642/251‑1 (MQ=255)
                                    gctaCAGTTGGCGATGAACCATGAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGGGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTa                                                                                                                                                           <  2:391872/248‑1 (MQ=255)
                                                 aTGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTAACTGCAACGAAGACGTGAACCGTGATTTTGCTAGCCTGCGTGCCACGTGGGAATCCGTAAAAGCCACCGGGAATGCCACACACTTTGATGACTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCACAAAGCCTGGATGGAGCTTATCGCTGACGTCGGTGGCGAAGGACAGATTTTCTATCGCCGCCGCCGt                                                                                                                                              >  1:399509/1‑251 (MQ=255)
                                                    aaCCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTcgc                                                                                                                                           >  2:322806/1‑251 (MQ=255)
                                                     aCCATTAAACCCGGAGCATCTCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTCTTTTTGCTGGCCTGCTTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGTCGAAGGTCAGATTTTCTATCGCCGCCGCTGTcgcc                                                                                                                                          <  2:104284/251‑1 (MQ=255)
                                                                      aTCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGCATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGc                                                                                                                         >  1:199728/1‑251 (MQ=255)
                                                                                          ccTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTg                                                                                                     >  2:215588/1‑251 (MQ=255)
                                                                                                tGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCg                                                                                               >  1:172063/1‑251 (MQ=255)
                                                                                                              gAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGt                                                                                 >  1:326435/1‑251 (MQ=255)
                                                                                                                 ccGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAg                                                                                  >  2:206364/1‑247 (MQ=255)
                                                                                                                 ccGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAg                                                                                  <  1:206364/247‑1 (MQ=255)
                                                                                                                     gtTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTa                                                                          <  1:344816/251‑1 (MQ=255)
                                                                                                                       tgttGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACAGTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGGCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTAc                                                                         <  1:398069/248‑1 (MQ=255)
                                                                                                                          tGCTGGCCTGCGTGCAACGTGGGAATCAGGAAACGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTCTAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGCCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGGACTCTCGTTGACTTCAGCCGTCGCTGGGGCCGCCAGTACAGATACAtgg                                                                     >  2:395997/1‑251 (MQ=255)
                                                                                                                                   gggagcactgTGGGAATCAGTAAAACCTACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTTACAGTTAGAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAATGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGCAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGTTGGGGCATCCAGTACAGCTACATGGTGGTGCTg                                                             <  1:295458/241‑1 (MQ=255)
                                                                                                                                        cAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACTCTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCATAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCt                                                       <  1:179898/251‑1 (MQ=255)
                                                                                                                                                     aGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGa                                          <  1:134546/251‑1 (MQ=255)
                                                                                                                                                     aGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGAAAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGGCAGATTTTCTATCGCCGCCGCCGTCGCCGCGGGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCTGTAATGa                                          <  2:258258/251‑1 (MQ=255)
                                                                                                                                                        aaaaGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCg                                       >  1:280132/1‑251 (MQ=255)
                                                                                                                                                        aaaaGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGGTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGAGGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCg                                       <  2:84321/251‑1 (MQ=255)
                                                                                                                                                                     aaTGCCAAACACGTTTATGTCTACATTCTTAGTGACAGTGATAACTTGGATATCTGCGTCACAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGAAGCCAGTACAGCTACATGGTGGTGCGGGATGCTGACTCGGTAAGGTCCGGTGATTGTTtgtg                          <  2:126304/251‑1 (MQ=255)
                                                                                                                                                                               acTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGGGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTg                >  1:16634/1‑251 (MQ=255)
                                                                                                                                                                               acTTTGATGTCTACATTATTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGCTGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTAGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGCCCGGGGATTGTTTGTGCGGGCTGGTg                >  2:139699/1‑251 (MQ=255)
                                                                                                                                                                                          tACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAACAAGCATGGATGGAGCTAATCGCTGAAGTCGGAGGCGAAGGACAGATTTTCTATCGCCGCAGCCGTAGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTAAATGGTGGAGCTGGCTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATgg      >  1:378792/1‑250 (MQ=255)
                                                                                                                                                                                             aTTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGc  >  1:399233/1‑251 (MQ=255)
                                                                                                                                                                                                cTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGAt                                                                                                             <  2:236801/141‑1 (MQ=255)
                                                                                                                                                                                                cTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGAt                                                                                                             >  1:236801/1‑141 (MQ=255)
                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                    
CTGCTTATTGGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGC  >  NC_000913/1111517‑1111956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑