Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,423,404	G→T	R2L (CGA→CTA)	rzoR →	putative prophage outer membrane lipoprotein RzoR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,423,404	0	G	T	100.0%	19.8 / NA	17	R2L (CGA→CTA)	rzoR	putative prophage outer membrane lipoprotein RzoR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (7/10);  total (7/10)

AGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGGGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGAGATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTT  >  NC_000913/1423189‑1423562                                                                                                                                                                                                                        |                                                                                                                                                               gtcaccgcgattATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGTTGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGc                                                                                                                             >  1:210515/12‑251 (MQ=11)    accgcgattATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGt                                                                                                                                >  1:202739/9‑245 (MQ=11)    accgcgattATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGt                                                                                                                                <  2:202739/237‑1 (MQ=11)            tATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGgtcgt                                                                                                                   <  1:202587/250‑1 (MQ=11)                 ccGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGgtcgtcg                                                                                                                 <  2:111449/247‑1 (MQ=11)                                  atcatcgtcTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGATGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGt                                                                                            >  1:383440/5‑251 (MQ=11)                                                           ttAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGCAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGccacc                                                                   >  1:330743/1‑251 (MQ=11)                                                                 atgaCCGTGATAACCCCATTACCATCAAAGCCCAGCGCGACAAAAATGCCAGAAAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATTTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTATAGCGTAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCTCACCAAAGCAGTCTGGCAGTCAGTGCGTGAAGCCCCCACCGcc                                                             <  2:330743/248‑1 (MQ=11)                                                                            aaCGCCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACGGAAGCTGGCGAACGCGGCAATTACGGACATGCAGATGCTTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTGGCCGCTGGTCGTCGTCGGTTGCACATCAAAGGGGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTgg                                                   <  2:132001/250‑1 (MQ=11)                                                                               ccaattacccacaaagcccagtgctaCAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGAGGTTGCTGCGGTCGATGCAAAATACACGATGGAGTTAGCTTAAGCTAAAGCTGAAAATGATGCCCTGTGTGATGATTTTGCCGCTGTTCGTCTTCGGTTGCACATCAAAGCAGTCTTTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATaa                                               <  1:38158/226‑1 (MQ=11)                                                                                              ccccAGCGCGCCAAAAATGCCAGAGAACTGAAGCGGGCTAACGCGGTAATTACTGAAATGCAGAGGCGTAAGCGTGATGTTGCTGCGCTCGATGAAAAATACACTAAGGAGTTAGCTGAGTCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGGCGCCGGTTGCCCATCAAAGCAGACGGTCAGCCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCg                                <  2:383440/248‑1 (MQ=11)                                                                                                       taCAAAAATGCCAGAGAGCTGAAGCTGGCGAACGCGGCAATTCCTGACATGCAGATGCGTCAGCGTGAGGTTACTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAACCTGAAAATTATGCTCTGCGTGATGATGTTGGCGCTGATCGTCGTCGGTTGCACATCAAAGCAGTCTGCCAGTCAGTGCGGGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCgcagtga                          >  2:273839/2‑242 (MQ=11)                                                                                                         cAAAAATGCCAGAGAACGTAAGCGGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGGTGCCGCTGGTCGTCGTCGGTTTCTCATCAAAGCAGTCGGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGaca                     <  1:398690/251‑1 (MQ=11)                                                                                                         aaaaaagtccAGAGAACTGAAGCTGGCGAACGCTGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCGGCGTTCGATGCAAAATACACGAAGGATTTAGCTGATGCTAAAGCTGAAAATGAGGCTCTGCGTGATGATGTTGCCGCTGGTCGTGCTCGGTTGCACATCAAAGCAGTCTGTCAGTCATGGCGTGAAGCCCCAACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGaca                     <  2:95236/243‑1 (MQ=11)                                                                                                              aTGCCAGAGAACTGAAGCGGGCGAACGCGGCAATTACTTTCATGCAGATGCTTCAGCGTGATGTTGCGGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCTCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCt                <  1:394977/251‑1 (MQ=11)                                                                                                                            aaGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTAttt  >  2:350847/1‑251 (MQ=14)                                                                                                                                                       atgcagatgcGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACCCGAAGGAGTTAGCTGATGCTAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACGTCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGCTAATGCAGCCTCCCCCCGACTGGCAGACACCGCt                >  1:194117/1‑210 (MQ=9)                                                                                                                                                                                                                        |                                                                                                                                                               AGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGGGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGAGATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCTGTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGACTGGCAGACACCGCTGAACGGGATTATTT  >  NC_000913/1423189‑1423562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑