Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,401,893 G→A Q455* (CAA→TAA)  abgB ← p‑aminobenzoyl‑glutamate hydrolase subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,401,8930GA100.0% 77.2 / NA 24Q455* (CAA→TAA) abgBp‑aminobenzoyl‑glutamate hydrolase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/12);  total (12/12)

GTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAAGCACGTTATCGGTGGCGGCATATGGAGCGAC  >  NC_000913/1401656‑1402139
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      
gTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAAt                                                                                                                                                                                                                                           >  2:58153/1‑251 (MQ=255)
           aaaTAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATc                                                                                                                                                                                                                                >  1:252134/1‑251 (MQ=255)
               aGATAAAGAGCAGAAAAGGATGGGGAACCTTGTGACCAATTCTTGCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTGGTTGTGTTTGCGTTGTTGTTGTTATGTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTGGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAg                                                                                                                                                                                                                             <  1:181064/250‑1 (MQ=255)
                gATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGCTTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAgg                                                                                                                                                                                                                            >  2:346375/1‑250 (MQ=255)
                            aaaaGGATGGGGAACCTTGTTACCACTTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGCTGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCCAGGAAGAGATTGACtg                                                                                                                                                                                                               >  2:57886/1‑251 (MQ=255)
                                                 aCCAAGTCTTTCGACCCAGCCATAGAGCTTCACGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTGGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTc                                                                                                                                                                                           <  2:193843/250‑1 (MQ=255)
                                                   cAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTcgcc                                                                                                                                                                                        <  2:214117/251‑1 (MQ=255)
                                                                        agagCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATg                                                                                                                                                                    >  1:72012/1‑250 (MQ=255)
                                                                                                 cgGTATGGTTGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGCTGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGACGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGcc                                                                                                                                                   >  2:338558/1‑242 (MQ=255)
                                                                                                 cgGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGGGTCCGTTACGTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGcc                                                                                                                                                   <  1:338558/242‑1 (MQ=255)
                                                                                                  ggTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGAGACGGTGTCACGTATTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAAc                                                                                                                                         >  1:176872/1‑251 (MQ=255)
                                                                                                        gATGAGATTCTCATGGGTATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGTTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGCTTGACGGTGGGTGCTGCCAGAGTTTTCGCCGCCAGCAGTATTCCTGGATGATCAATAGATGCTCGCCCCTGGCGACCCAGTTg                                                                                                                                   <  2:235978/251‑1 (MQ=255)
                                                                                                                             ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTAcc                                                                                                              <  1:137696/251‑1 (MQ=255)
                                                                                                                                                gttgttgttgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTg                                                                                           <  1:172942/251‑1 (MQ=255)
                                                                                                                                                                 aaaGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAgg                                                                          >  1:124445/1‑251 (MQ=255)
                                                                                                                                                                                         gggATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGAc                                                  <  1:91671/251‑1 (MQ=255)
                                                                                                                                                                                           gATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACAt                                                <  1:58153/251‑1 (MQ=255)
                                                                                                                                                                                             tAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAACCAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATc                                               >  1:216947/1‑250 (MQ=255)
                                                                                                                                                                                                   ggtgtaaCGGTGGCGAGTCCGTTACATGCTGATGCTCTGGTGATCATTCTTTCAATAGCCCTGAATCAAGGAAGAGATTGTCTGTGGTTGCGGCCATATTTTTCGCCGCCAGCATCTTTCCTTTATGAGCAATAGATCAGCGCCCCTGGCTAACCAGGTGCCACGTATGTAGCGGTGTACTCACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTCCCAACTGACGCCGCCGGCATCAGTCGAt                                        <  2:335169/245‑1 (MQ=255)
                                                                                                                                                                                                               gCGTGTCCGTTACGTGCGTATGATATTGTTAGCATTCTTGCAATAGCCCTGAAGCAAGGAAGATATTGCCTGTGGTTGCAGCCATAGTTTTCGCCGCCAGCAGCATTCCTGTATGAGCAATTGATGTTCGCCCCTGGCTAACCGGTTGCCACGTATGTAGCGTTGTACCTGCGGCAAATCAGGTGCTGAAACACTGGGCAACAGGCAGTTTCCAACTTACGCCGCTGACGTTAGTCGAGGCCGCAAgaac                             <  2:42502/250‑4 (MQ=255)
                                                                                                                                                                                                                           cTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGCAGCGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAg                                                                                                 >  2:50288/1‑170 (MQ=255)
                                                                                                                                                                                                                           cTTGCTGATGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAg                                                                                                 <  1:50288/170‑1 (MQ=255)
                                                                                                                                                                                                                                   tGCTCTTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGTCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCTACATCAGTCGTTGCCGCAAGCACGTTATCGCTGGCGGCATATgg        >  1:356924/1‑251 (MQ=255)
                                                                                                                                                                                                                                     ctctTGTTAGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATCGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAAGCACGTTATCGGTGGCGGCATATgg        >  2:164894/1‑249 (MQ=255)
                                                                                                                                                                                                                                         tgttaGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGAAGCATTCCTTTATGCGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGCCGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAAGCACGTTATCGGTGGCGGCATATGGAGCGAc  >  1:318979/1‑251 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      
GTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAAGCACGTTATCGGTGGCGGCATATGGAGCGAC  >  NC_000913/1401656‑1402139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑