Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,859	T→C	Y110C (TAT→TGT)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,859	0	T	C	93.1%	83.8 / ‑6.1	29	Y110C (TAT→TGT)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (9/18);  minor base A (1/1);  total (10/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

CCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATC  >  NC_000913/1358622‑1359104                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      ccAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGa                                                                                                                                                                                                                                          <  1:48596/251‑1 (MQ=255)  cAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAAACATCGGCAAAAAGAGACAATCCAGCAGCGAt                                                                                                                                                                                                                                         <  2:234646/251‑1 (MQ=255)      gCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGAcc                                                                                                                                                                                                                                     <  1:114147/251‑1 (MQ=255)          tctcCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACCCCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCCCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGTCAAAAAGAGACAATCCAGCAGCGATGACCAGc                                                                                                                                                                                                                                  <  1:129415/250‑1 (MQ=255)                                       gAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAACACCCCAACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGAAAGGTTTTCGCCACCAATACGGTAATCCTCGGAACAAAGAGACCATCCAACAGCGATTACCATCCGGACATAAATCCCACATGGGGGATAATCg                                                                                                                                                                                                    >  1:230281/1‑251 (MQ=255)                                                  ccaccaTGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCCCCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTTGCCAACAAGACGTTTATACTCGGCAAAAAGAGACAATCAAGCAGCGCTGACCAGCCGACCATAAATCCGACGTGCGGGATAATCGACTTTTTcgcg                                                                                                                                                                                         >  1:213381/1‑251 (MQ=255)                                                         gatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCTGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGCCGTGCGGGTTAATCGACTTTTGCGCGTAGGtat                                                                                                                                                                                  <  1:17338/251‑1 (MQ=255)                                                         gatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGtat                                                                                                                                                                                  <  1:250779/251‑1 (MQ=255)                                                         gatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGta                                                                                                                                                                                   <  1:259100/250‑1 (MQ=255)                                                            ggAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTTCGCGTAGGTATAgg                                                                                                                                                                               <  1:321134/251‑1 (MQ=255)                                                               gaTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGGGGAGAGATAGATTTTCGCCAACAAGAAGTTAATCATCGGCAAAAAGAAACAATCCAGCAGCGATGACCAGCCGCACATAAATCCGACGTGGGGGTTAATCGACTTTTGCGCGTAGGAATAGGCCg                                                                                                                                                                            >  1:219706/1‑251 (MQ=255)                                                                  ttGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTGCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGCAGGTATAGGCCGaa                                                                                                                                                                          <  2:59642/250‑1 (MQ=255)                                                                        cAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCt                                                                                                                                                                   <  2:219706/251‑1 (MQ=255)                                                                            aCAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGATCCGGCCTCCgg                                                                                                                                                               <  2:54707/251‑1 (MQ=255)                                                                               aaCAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATc                                                                                                                                                                                                                      <  1:269315/193‑1 (MQ=255)                                                                                           aaGTTAGCGCCCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGAAGAAGGTTAACACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGGTAGATTTTCGCCCACAAGACGGTTATCATCGGCAAAAAGAGAACATCCAGCAGCGATGACCAGCCGACCCTAAAACCGACGTGCGGGGTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACAGGCGAAcccg                                                                                                                                                >  2:186285/1‑249 (MQ=255)                                                                                               tAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGttt                                                                                                                                             <  1:38859/250‑1 (MQ=255)                                                                                                                     cAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGGTGACCAGCCGACCATAAATCCGACGGGCGGGGTAATCGACTTTTGCGCGTAGGGATAGGCCGGACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAAc                                                                                                                       >  1:254982/1‑250 (MQ=255)                                                                                                                             cggcggTTAAAATGGCGACGAAGGTTACCACCCCCACCCACGGCGGCACTTCCGGGGAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTTCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACAccc                                                                                                              >  1:73227/1‑251 (MQ=255)                                                                                                                                                gaAGGTTACCCCCCCCACCCCCGGCGGCCCTTCTGGGAAGAGGCCGGAGAGATAGTTTTTCTCCACCATAACGTTAATCATGGGCAAAAAGAGCAATTCCAGCAGCGATGACCAGCCGCCCATAAATCCGACGTGCGGGTTATTCGCCTTTTGCGCGTGGGTATAGGCCGATCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCTTTAAACAGCACACCCGCCAGCGCCAGCAAATAgg                                                                                           <  1:80321/251‑1 (MQ=255)                                                                                                                                                                           cctttCCGGGAAGAGGGCGGAGAAATATATTGTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACATCCCAGCATCGATGACCAGCCGCCCATAAATCCGACGTGCGTGTTAATCGACTTTTGCGCGTAGTTATAGGCCGAACCGCCCTCCGGAAACTGGCGAACCATTTTGCCGTAGCTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGc                                                                <  1:376038/248‑1 (MQ=255)                                                                                                                                                                            actcccGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGAAAAAAGAGACAACCCAGCAGCGATGACCAGCCGACCATAAACCCGACGTGCGGGTTAATCTACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGcc                                                               <  1:131513/247‑1 (MQ=255)                                                                                                                                                                            aCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATACAGCAGCGATGACCAGACGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAAACAGTTTGCCGTAGCTGCTAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGcc                                                               >  1:265539/1‑251 (MQ=255)                                                                                                                                                                                          ggCGGAGAGAAAGATTTCCGCCAACAAGCCGTAAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAACCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAACCGGGCGAACCATTTGGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCGAATGCCGGAGACAATACCaa                                                 <  1:126886/251‑1 (MQ=255)                                                                                                                                                                                                  aatagatTTTCGCCAACAAGACTTAAATCAACGGAAAAAAGAGACAACCCAGCAGCGAGGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCTTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGACCCATTTTGCCGTAGCTGAGAGCGGTAAACACCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCa                                         <  1:41463/250‑1 (MQ=255)                                                                                                                                                                                                   atcgatTGTCGCCATCCAGACGTTAATCATCGGCAACACGAGCCAATCCAGCAGCGATCACCAGCCCACCCTAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCAAAACCGCCAGCGCCAGCAAATCGGACGCCGGAACGTGACCGTCGCTAATGACGGAGACAATACAAAAAGTAtaa                                         >  2:250767/4‑248 (MQ=255)                                                                                                                                                                                                               caacaaGACGTTAATCATCGGAAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAACCTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCg                            <  1:48280/251‑1 (MQ=255)                                                                                                                                                                                                                aacaaGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACAAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGCACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGAGCCAGCAAATAGGACGACGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCgg                           >  2:327782/1‑251 (MQ=255)                                                                                                                                                                                                                                         agagACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCatcatc  >  1:256902/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      CCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATC  >  NC_000913/1358622‑1359104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑