Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,357,2020CT92.0% 66.1 / NA 25intergenic (‑92/+221)sapA/ymjAputative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (12/11);  minor base G (1/1);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAG‑ATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAT  >  NC_000913/1356970‑1357420
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                          
aaaGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGGCGCGGATATCAGCATGCGGGGGAG‑ATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATCCCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGGCGTTGa                                                                                                                                                                                                           <  2:33038/250‑1 (MQ=255)
                    tGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAG‑ATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGCTAATACCTGGCGCCTAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGt                                                                                                                                                                                      >  1:329782/1‑251 (MQ=255)
                      aCGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAG‑ATTCAGGCGCGGCGATTGCCTGACAACTCACAAGTCCAGCAATCAACAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGACCAGGTGACGTCGCCATCTGTTc                                                                                                                                                                                    >  1:41522/1‑251 (MQ=255)
                                     ccGCTGTCGCGGAGATCAGCATGCGGGGGAG‑ATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGTATAATATTAAGGGATGTTAAGTAAAGAGGCTATCTTACTAATATTTAAGGACATTTGCCAGTACCGTTTGTGGTCAGGGGTCGTAATGAGGGCTGTGGGGTAGGTCGTTGAACAGGTGACGTCGCCATCTGGGCAACATTCGTACCCGt                                                                                                                                                                     <  2:402385/251‑1 (MQ=255)
                                               ggATATCAGCATGCGGGGGAG‑ATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTctct                                                                                                                                                            >  2:32017/1‑250 (MQ=255)
                                                 atatCAGAATG‑GGGGGAGGATTCAGGCGCGGCGATTGCCTGACCACTAACAAGACCCGCACTCACCAAAAGCGACGATAATACCTGGAGCATACTATTAAGGGATTTTATGTAAAGAGGATATATTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGAAATGAGGGCGGTGTGGTTGGTCTTTGAACAGGTGACGTAGCCATTTGTTCACAATTCGTACCCGTGTTTTCTctat                                                                                                                                                          >  2:335133/1‑250 (MQ=255)
                                                      aGCATGCGGGGGAG‑ATTCAGGCGCGGCGATTGCCTGCCCACTCACAAGGCCAGCAATCACCAAAAGAGACGATAAATCCTAGCGCATAATATTACGGGATGTTATGTACAGAGGCTATCTTACTAATATGTAATGACATTTGCCATTACCGTTTGTGTTCAGGGTTCGTAATTAGGGCTGTGTGGTTGGTCGTTGAACAGGTGGCGTCGCCATCTGTTCAGCATTCGTACCCGTGATTTCTctttaactag                                                                                                                                                    <  2:217092/251‑2 (MQ=255)
                                                               gggAG‑ATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAg                                                                                                                                           <  2:325876/251‑1 (MQ=255)
                                                                                            ccACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTAc                                                                                                               >  2:155111/1‑251 (MQ=255)
                                                                                                    aaGTCAAGCACTCACCAAAAGAGACGATACTACCTGGCGCATCATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTg                                                                                                        >  2:383626/1‑250 (MQ=255)
                                                                                                                 aCCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTaaa                                                                                          <  1:383626/251‑1 (MQ=255)
                                                                                                                      aaGAGACGATTATACCTGGTGTATAACAGTAAGGGATTTTATTTAAAGAGGTTATCTTACTAATATTTAATGACATTTGCCAGTTCCGTTTGTGTTCAGGGGTCGTAATGAGTGTGGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCTCAGAACAAATTAGCGAGGAGCAGAGCGGTCCGTCCGTTAGAAACTCATTAAAGCACg                                                                                     <  2:354290/251‑1 (MQ=255)
                                                                                                                         agaCGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTg                                                                                  >  1:28700/1‑251 (MQ=255)
                                                                                                                              ataataCCTGGCGCATAATATTAAGGGATTTTATGTAAAGATGCTATCTTACTAATATTTAATGACCTTTGCCATTACCGTTTGAGTTCAGGGGGCGTAATGAGGGCTGTGTGGTTTGTCGGTGAACAGGTGACTACGCCACCTCGCCAACATTCGTACCCGTGATTTCGCTATAACTATATCTCACAGATCAACTTAGCgagga                                                                                                                           <  2:40339/205‑1 (MQ=255)
                                                                                                                                  taCCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCgtg                                                                         >  1:56478/1‑251 (MQ=255)
                                                                                                                                           cATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTa                                                                <  2:96998/251‑1 (MQ=255)
                                                                                                                                              aataTTAAGGGATTTTATGTAAAGCGGCTATCTTACTAATCTTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGcc                                                             >  2:231372/1‑251 (MQ=255)
                                                                                                                                                   taagggcgtttATGTAAAGAGGCTATCTTACTAATATTTACTGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGAGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTAGTCCCCGTGATTTCTCTATAACTATAACTCACAGAACAACATAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTACAGAACGCTGCAGTGCGCGTAATTGTTAGcacgatg                                                        >  2:244825/9‑245 (MQ=255)
                                                                                                                                                                                acgaaTATTTAATGACATTTGCCCTTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTGGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTGAGCGAGGAGCAGAGCGGTACTATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCCTGTCATTGCTAGCCAGATGCGGCTTGTACGCTTTATCCGGCCAACGa                            <  2:41522/247‑1 (MQ=255)
                                                                                                                                                                                      atTTAAGGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTGAGCGAGGAGCAGAGCGGTACTATCGTGAGAAACCCATTAAAGCGCGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGa                      <  1:400170/250‑1 (MQ=255)
                                                                                                                                                                                                   tGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGATACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCa         >  1:90869/1‑250 (MQ=255)
                                                                                                                                                                                                    gCCATTACCGTTTGTGGTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACGCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAGCCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAGCGCTGTATCCGGACAACGATACGGGCCGATCGTCTGCAAt       <  2:329782/251‑1 (MQ=255)
                                                                                                                                                                                                      cATTACCGTTTGTGTTCAGGGGTCGTAATGCGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGAGTCATTGTTAGCCAGATGCGGCGTGCACGCTTTCTCCGGCCACCGATACTGACCGATCGTCTGCAAtt      >  1:389923/1‑250 (MQ=255)
                                                                                                                                                                                                       aTTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTaa    <  1:155111/251‑1 (MQ=255)
                                                                                                                                                                                                         tACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAt  >  1:170251/1‑251 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                          
AAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAG‑ATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAT  >  NC_000913/1356970‑1357420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑