Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,322,759	G→A	A63V (GCT→GTT)	trpE ←	anthranilate synthase subunit TrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,322,759	0	G	A	96.2%	77.9 / NA	26	A63V (GCT→GTT)	trpE	anthranilate synthase subunit TrpE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (7/18);  minor base C (1/0);  total (8/18)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAGCTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGG  >  NC_000913/1322526‑1322993                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           gTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAGGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATgcgcagcgca                                                                                                                                                                                                                           <  1:73518/251‑1 (MQ=255)      tccttcGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTg                                                                                                                                                                                                                        <  1:380359/249‑1 (MQ=255)      tccttcGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTg                                                                                                                                                                                                                        <  1:276627/249‑1 (MQ=255)         ttcGGTACATTCAACAGATTCTGCAATCAACGGAAAGCGTCAAAAACCGACAGGGCGCATAAGCGGCCGTCTTCATCCAGCAGCGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTCATGCGCAGCGCACTGTCTAc                                                                                                                                                                                                                   >  2:152435/1‑251 (MQ=255)                 atgcAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGTAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGTGCAGTGCACTGTCTACcagcagca                                                                                                                                                                                                           <  1:350054/248‑1 (MQ=255)                                                           aaGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCca                                                                                                                                                                 <  1:322611/251‑1 (MQ=255)                                                                     aaGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCg                                                                                                                                                        <  1:132127/250‑1 (MQ=255)                                                                             gTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCCGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGCGCGTTATCCAGAAGTGCCAGGAGGGCTTCGCCGTTGCCGGACAGTGCCTGGATTGCGACAGTGTAACCTAAACCTGTAATGCGCAGCGCACTGTCTACCCGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCAGGCAGCAGCGTTGCCgggc                                                                                                                                               >  2:403023/1‑249 (MQ=255)                                                                              tCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTTCCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACg                                                                                                                                              >  2:19123/1‑251 (MQ=255)                                                                                     ccAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCCGTTTGGTGATTGCTCACTTTCCACACCCGCAGGCACGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATcccc                                                                                                                                        >  2:196791/1‑250 (MQ=255)                                                                                       agcagTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCcac                                                                                                                                      >  1:273672/1‑250 (MQ=255)                                                                                         cagTGGACTGACAGGGGGGAAGCGAAGCACGCGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGGGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCcacaca                                                                                                                                   <  2:150747/251‑1 (MQ=255)                                                                                                       gggggAAGCGCAGCACACGGCCGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAgc                                                                                                                     >  2:225493/1‑251 (MQ=255)                                                                                                           gAAGCGCAGCAAACGGCAGTTTAGTCATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGACTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGGTCCCCCCACAACTGGCGAAAAAGAGCgg                                                                                                                 >  2:364721/1‑251 (MQ=255)                                                                                                              gcgcAGCACCCGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCGTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTgg                                                                                                              <  2:105571/251‑1 (MQ=255)                                                                                                                                        gTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTGGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAg                                                                                    <  2:304164/251‑1 (MQ=255)                                                                                                                                                    cacCCGCGTGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGGGCCGGGATTTGGACAGTGGCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCATGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGTAGTTCg                                                                        <  2:220978/251‑1 (MQ=255)                                                                                                                                                            ggcaggGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCgg                                                                <  1:355598/251‑1 (MQ=255)                                                                                                                                                                               gtGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGtt                                             <  1:52566/251‑1 (MQ=255)                                                                                                                                                                                  ccAGGAGGGCTTCTCCTTTGCCGGAAAGTGCTTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCGACTAGCAGCAGGCTTTTTAAATCATCTGTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGGTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCTCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGGTTTTGTGTTTGCATTTTTAtt                                          <  1:337271/251‑1 (MQ=255)                                                                                                                                                                                       aggGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTa                                     <  1:225493/251‑1 (MQ=255)                                                                                                                                                                                        gggCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTTACAGTGTCACCGAAAACTGTAAGGCGCAGCGCACTTTCTACCAGCAGCAGGCTTTTTAAAGCATCTTTGCTGTCTATATCTGCGGATTCCAGCAGCAGCGGTTCCGGACGATCCCCACACAACTGGTGAAAAAGCGCTGTGTGATTGTCGCGATAAGCGCCTTCGCAGGTTTGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTaa                                    <  1:18565/251‑1 (MQ=255)                                                                                                                                                                                              cGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGGAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGt                              >  1:370486/1‑251 (MQ=255)                                                                                                                                                                                                ccGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTc                            <  1:152435/251‑1 (MQ=255)                                                                                                                                                                                                                        gtgACAGTGTCACCTAAAACTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGc     <  1:358434/250‑1 (MQ=255)                                                                                                                                                                                                                          gACAGTGTCACCTAAAACTGTAATTCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCTGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCggg  <  1:283435/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           GTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGGACTGACAGGGGGGAAGCGCAGCACACGGCAGTTTGGTGATTGTTCACTTTCCACACCCGCAGGCAGGGCGTTATCCAGTAGTGCCAGGAGGGCTTCGCCGTTGCCGGAAAGTGCCTGGATTGTGACAGTGTCACCTAAAGCTGTAATGCGCAGCGCACTGTCTACCAGCAGCAGGCTTTTTAAATCATCTTTGCTGTCGATATCTGCGGATTCCAGCAGCAGCGTTGCCGGACGATCCCCACACAACTGGTGAAAAAGCGCGGTGGGATTGTCGCGATAAGCGCCTTCGCAGGTTAGCAGTTCGAGAGTCGGTTTTTGTGTTTGCATTGTTATTCTCTAATTTTGTTCAAAAAAAAGCCCGCTCATTAGGCGGG  >  NC_000913/1322526‑1322993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑