Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,310,770	A→G	H32H (CAT→CAC)	yciI ←	protein YciI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,310,770	0	A	G	100.0%	42.0 / NA	13	H32H (CAT→CAC)	yciI	protein YciI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/7);  total (6/7)

CAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCATGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACAT‑ACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTT  >  NC_000913/1310570‑1311014                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      cAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGttt                                                                                                                                                                                                     <  1:120475/251‑1 (MQ=255)                aagggTTTCACTGAACCGTGTGCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGc                                                                                                                                                                                      <  1:132589/248‑1 (MQ=255)                                   gttgatataCTCCTTCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGTTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCAGTTGACCAGCTGTCAACAAACGTCCTGCATCGGGCAGTAACTGTAAACGTTCGAAAGGTGCCGGACGAACGGAAAGGCGTGTTGCGAGGCTATCAGCTTTGTCTTGAGCGTAAATa                                                                                                                                                                   <  2:401458/243‑1 (MQ=255)                                                            cgGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGATAATGTCTACCGATGGCATTGGACCAGCTGTCAACAACCGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCGATCAGCTTTGTCTTAAGCGTAAATAAAAT‑ACAACACGGACACAACTCCtt                                                                                                                                         >  2:129282/1‑251 (MQ=255)                                                               gttaGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATGCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCGGGATCTTTACTGTCTTCCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGCCTTGAGCGTAAATAACAT‑ACAACACGGGCACAACTCCTTGtt                                                                                                                                      <  2:341129/248‑1 (MQ=255)                                                                        ggCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACAT‑ACAACACGGGCACAACTCCTTGTTCGGGAAAGt                                                                                                                             <  1:326259/251‑1 (MQ=255)                                                                          cccAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACAT‑ACAACACGGGCACAACTCCTTGTTCGGTAAAGTTg                                                                                                                           <  1:116037/251‑1 (MQ=255)                                                                                  tGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAA‑TAAAATTCCAAACCGGGCCCAACTCCTTTTTGGGGAAAGtggaaatgaac                                                                                                                   >  2:213478/1‑241 (MQ=255)                                                                                                                                      ccGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACAT‑ACAACACGGGCACAACTCCTTGTTCGGGACAGTTGTAAAGAACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTaa                                                                 >  2:306349/1‑249 (MQ=255)                                                                                                                                         cAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACAT‑ACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGcc                                                             >  2:313055/1‑250 (MQ=255)                                                                                                                                          aGCCCCTGGATCGTTACTGTCTCCCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACAT‑ACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTAGTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAATGCCAt                                                           <  2:365112/251‑1 (MQ=255)                                                                                                                                                tGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACAT‑ACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGAACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATa                                                      >  1:214565/1‑250 (MQ=255)                                                                                                                                                                                                aacTTCATCGTACAATAATTGTAAACGTGCTAAATGTGCCGGACCAACGGAAAGGCGTTTTTCGAGACTATCAGCCTTTTATTGAGCGTAAATAAAAT‑ACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGAACGTTATCTGAAAGGGCGAAGATCAGCAAAGGAAAGATGATGTCATTGTTAAGGCCATGCATAAAGTAAGGGTAATTCCGCCAAAAATGACATTTTAACTGATCCTGATCGt     >  2:248932/3‑251 (MQ=255)                                                                                                                                                                                                    tcatcgTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACAT‑ACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAATGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCtt  <  1:180481/250‑1 (MQ=255)                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      CAGAACACTTTCTTAAATGGTTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCATGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACAT‑ACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTT  >  NC_000913/1310570‑1311014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑