Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,306,7360TG95.5% 52.5 / ‑3.4 22S325A (TCC→GCC) oppFmurein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (8/13);  total (9/13)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-01

GTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAA  >  NC_000913/1306493‑1306958
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              
gTAGAACTGGGGACCTATGCTGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCATTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTc                                                                                                                                                                                                                         >  1:223430/1‑251 (MQ=255)
     aCTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGTAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATGGTCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGcc                                                                                                                                                                                                                    <  1:240468/251‑1 (MQ=255)
        ggtgcccTAGGATGAGGTCTAACACATGCCTCTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTTATCCTGTTCTGTAGAATAACAAAACCATCCATTTACTGTAAGGGGAATTGCCGTCGCCGATCAACCCGCCTTCCTGTTGTTTTTTCCGTACCCGTTGCCTGTTTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACTCGCCGTTTCTTGCCTg                                                                                                                                                                                                                  <  1:357095/245‑1 (MQ=255)
        ggggACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTg                                                                                                                                                                                                                  >  1:286535/1‑250 (MQ=255)
                  gatgaGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATcc                                                                                                                                                                                                       <  1:32420/251‑1 (MQ=255)
                  gatgaGGCCTACCACAATCCACTACACCCTTACACCAGGGCCTTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGCACACAACCATCCAGTTACTGGAAGGGTAATTACCGTAGCCGTTCAACCCGCCTTCCGGTTGTGTTTGCCGTACCCGTTGCCCGAGTGCCGGTCCAGTGTGCGCCAAACCAAGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATcc                                                                                                                                                                                                       >  2:324609/1‑251 (MQ=255)
                       ggacTACCACAATCCAATACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGCTCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGACTTACCGTCGCCGATCAACCAGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCttt                                                                                                                                                                                                  >  2:177668/4‑251 (MQ=255)
                       ggTCTACCACAACCCACTACATCCTTACACCAGGGCATTGAGGTCGGCAGTCCCCATCCCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGTAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCttt                                                                                                                                                                                                  <  1:305952/251‑1 (MQ=255)
                        gTCTACCTCAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGTGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTa                                                                                                                                                                                                 <  1:348415/251‑1 (MQ=255)
                                 aaTCCACTACATCCTTACACCAGGGCATTGATGTCGGCATTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTTCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATa                                                                                                                                                                                        <  2:93780/251‑1 (MQ=255)
                                 aaTCCACTACATCCTTACACCAGGGCATTGATGACGGCAGTCCCCATACCTGATCCGGAGCTGGAGAAGAACAAACCCATCCAGTTTCTGGAAGGGTAATTCCCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATa                                                                                                                                                                                        <  1:45833/251‑1 (MQ=255)
                                  aTCCACTACCTCCTTACACCAGGGCATTTAGGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATGACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGTAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGGCGATCCGCTTTAAAAACAATaa                                                                                                                                                                                       <  2:360774/251‑1 (MQ=255)
                                          caccctgaccccAGGGAATGGAGGTCGGCGGTCCCCATACCTGACCGGGATCTTGAGAAGAACAAAACCCTCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCGTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAATCACGTCCTGTTCTGGAGGGGAGTTACAGACACGCCGTGTCTTGCCTGAAAGTCTATCCGCTTTAAAAACAATAAGGTCTGAc                                                                                                                                                                               <  2:387170/241‑1 (MQ=255)
                                                                 gTCGGCCGTCCCCACACCTGATCCGGACCAGGAGAAGCACAAAACCATCCAGTTACTGCAAGGGGACTTACCGTCGCCGATCAACACGCCTTCCGGGTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTGCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGCCAGTTGACAGCCCTTTTTCACGCt                                                                                                                                                        >  2:259358/1‑251 (MQ=255)
                                                                     gCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTCCCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGATTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTaaaa                                                                                                                                                    <  1:269727/251‑1 (MQ=255)
                                                                                                                                  aaTTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAATGTTTGTGATCTTTTTCGCGGCATAAc                                                                                        >  1:222911/1‑250 (MQ=255)
                                                                                                                                                    aCCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCATCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGata                                                                                                                         <  1:114143/199‑1 (MQ=255)
                                                                                                                                                    aCCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGata                                                                                                                         >  2:114143/1‑199 (MQ=255)
                                                                                                                                                                          ccGTACCCGTTGCCCGCTTGCCGGTACAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACt                                               >  2:232333/1‑251 (MQ=255)
                                                                                                                                                                                       ccGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCa                                  <  1:177668/251‑1 (MQ=255)
                                                                                                                                                                                                                    acGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCCTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAACAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGAGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGtt     <  1:94304/251‑1 (MQ=255)
                                                                                                                                                                                                                        ccTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCaa  >  1:169541/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              
GTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAA  >  NC_000913/1306493‑1306958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: