Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	246,747	G→T	S12S (TCG→TCT)	yafL →	NlpC/P60 family protein YafL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	246,747	0	G	T	100.0%	189.2 / NA	57	S12S (TCG→TCT)	yafL	NlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/23);  total (34/23)

GGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCTCAAG  >  NC_000913/246505‑246993                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       gggTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACt                                                                                                                                                                                                                                                <  1:322762/251‑1 (MQ=255) gggTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGCAGGTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTACTTATATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATCCACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGTATTACt                                                                                                                                                                                                                                                >  2:249572/1‑251 (MQ=255)             tgCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGAtttgttt                                                                                                                                                                                                                                    >  2:124324/1‑251 (MQ=255)                   atatTTATTCAGCTTGCATTGTCGATCAAATGAGAAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTAAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTATGGATTACTGTTGATTTGTTTGCCGtt                                                                                                                                                                                                                              >  2:247240/1‑251 (MQ=255)                        tattCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGGCAATCCATGAGTAATTTAATAAACGGATGTTTTTACATACTTAACTGAAATATCTATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTTCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTttctt                                                                                                                                                                                                                         >  2:287637/1‑251 (MQ=255)                             aGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCAttt                                                                                                                                                                                                                    >  2:169830/1‑251 (MQ=255)                               atttaaatggatcTCAAATGTGCAACATCGACAATCCATGAGTAATTTAATAAACGGAGGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTTGTCAGAAACGATTCGGGATACACTGCAACAGTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTTGATTACTGTGTATTTGTTTTCCGTTTTCTTCATTTGc                                                                                                                                                                                                                  <  2:247208/239‑1 (MQ=255)                                ttGAATTGTAGCTCAAATGAGCAACAGCAACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGTTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGCTTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGcc                                                                                                                                                                                                                 >  2:45882/1‑251 (MQ=255)                                             cacaTGAGCAACAGCGAAAATCCATGAGCAATTTCATAAACGGATGTTTTTACATAATTAAATGAACTATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACcacat                                                                                                                                                                                                    >  2:178609/4‑251 (MQ=255)                                             cAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATCTATATGTTAATTTTATAATACTGGTTTATTAAGTTTGTTCAGAAACGCTCCGGGATACACTGCAACATTAAAGCATGATCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGCTTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACcacat                                                                                                                                                                                                    >  1:205665/1‑251 (MQ=255)                                              aaaTGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACcacatc                                                                                                                                                                                                   >  1:48426/1‑251 (MQ=255)                                                     cAACAGCGACAATCCATGAGTAATTTACTAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGATTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCAGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTACATTTGCCAGCGCCACCACATCACATAtc                                                                                                                                                                                            >  2:143261/1‑251 (MQ=255)                                                      aaCAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATAtct                                                                                                                                                                                           <  2:150932/251‑1 (MQ=255)                                                        cAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCTACCACATCACATATctct                                                                                                                                                                                         <  1:374327/251‑1 (MQ=255)                                                                                    aCGGAAGTTTTTACATAATTACCTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACAATGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATCTCTCTTTCAGCTACGCcgcccgcctgctgctgc                                                                                                                                                             >  2:194754/1‑242 (MQ=255)                                                                                             tttACATAATTAATGGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCATAAAGTATCCGGCATACACTGAAACATTAAAGCAGTACCAGCCATTATTGAGTATTTCCTTGCCGTCGATTCCATCTTTTGTATGTTCTGGATTACTGTTCATTTGTTGGCCGTTTCCTTCATTTGCCAGCGCCATCACAGCACATATCTCGTTCAGGTACGCCGCCCGCCAGCGGATGCCAAACCGt                                                                                                                                                     <  2:250207/250‑1 (MQ=255)                                                                                               tacagaataatcttaaaTATATATGTTAATTTTATAATAATGTTTTATTAAGTTTGTTCAGAAACGTTCCGGGATACCCTGCAACTTTAAAGCATGACCAGCCATTATTGAGTATTTCCTTGCCGTGGATTCCTTCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCTCCACATCATGTATCGCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTgc                                                                                                                                                   <  2:293952/236‑1 (MQ=255)                                                                                                 cATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGcgcg                                                                                                                                                <  2:48426/251‑1 (MQ=255)                                                                                                    aaTTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCATCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGttt                                                                                                                                             >  2:5086/1‑251 (MQ=255)                                                                                                          cTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTttctt                                                                                                                                                                                                                         <  2:381598/169‑1 (MQ=255)                                                                                                          cTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTttctt                                                                                                                                                                                                                         >  1:381598/1‑169 (MQ=255)                                                                                                            aaaaTATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGCCCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTaaa                                                                                                                                       <  1:344096/248‑1 (MQ=255)                                                                                                                   atatGTTAATTTTATAATAATGGTGTATTAAGTTTGTTCAGAAACGATCCGGGATCCACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGGCTGGATTACTGTTGATTTTTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTAcc                                                                                                                               <  2:126319/250‑1 (MQ=255)                                                                                                                   atatGTTAATTTTATAAATATGTTTTATTAAGTTGTTTCAAAAACAATCCGGGATACACTGCAACATTAAAGAATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGGATTGTCTGGATTACTGTTGATTTGGTGGCCGTTTCCTTCATCTGCCAGCGCCACCACATCACAGATCTCTTTCAGCTACGCCGCCCGGCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTAcc                                                                                                                               <  2:132925/250‑1 (MQ=255)                                                                                                                       gTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACt                                                                                                                          >  2:67886/1‑251 (MQ=255)                                                                                                                        ttaataatataATAATGGATTATTAAGTATGTGCAGGAACTATCCGGGTTACTATGCAACATTACATCATGACTAGCAATTATTTAATCTGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATGACTGTTGACTTCTTTGCCGTATTCTTCTTTTGCCGCCGCCCCCACATCAAATATCTCTTTCAGCTACGCCGTCCGCCTGCCCTGGCAAACCCGTGCTCGTTTCTTACATCAGTAACAAACTc                                                                                                                         >  2:68261/8‑251 (MQ=255)                                                                                                                        ttAATTTTATAATAATGGTTTATTAAGTTTGTTCAGCAACGATCAGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTCCTCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTc                                                                                                                         >  2:298714/1‑251 (MQ=255)                                                                                                                          aaTTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCAt                                                                                                                       <  1:30544/251‑1 (MQ=255)                                                                                                                              ttATAATACTGGATTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCACACTCATCTg                                                                                                                    >  2:149822/1‑250 (MQ=255)                                                                                                                                  aataatGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaaa                                                                                                                >  1:113984/1‑250 (MQ=255)                                                                                                                                      atGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGACAAAGc                                                                                                             >  2:206108/1‑249 (MQ=255)                                                                                                                                       tGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTttcttc                                                                                                                                                                                                                        >  2:152391/1‑141 (MQ=255)                                                                                                                                       tGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCAGCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTttcttc                                                                                                                                                                                                                        <  1:152391/141‑1 (MQ=255)                                                                                                                                               taAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGCTGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAGAGCAGGCCAGCtat                                                                                                  >  1:350850/1‑251 (MQ=255)                                                                                                                                                 aGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTatat                                                                                                >  1:210403/1‑251 (MQ=255)                                                                                                                                                  gTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATAtt                                                                                               <  1:312478/251‑1 (MQ=255)                                                                                                                                                      gTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTgg                                                                                           <  1:114788/251‑1 (MQ=255)                                                                                                                                                         cAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTATTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAg                                                                                        <  1:12603/251‑1 (MQ=255)                                                                                                                                                              aCGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATtgt                                                                                             <  1:373824/241‑1 (MQ=255)                                                                                                                                                              aCGATCCGGGATACACTGAAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCCTCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACAGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCCGCTATATtgt                                                                                             >  2:373824/1‑241 (MQ=255)                                                                                                                                                               cgagccGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATg                                                                                  <  1:239200/247‑1 (MQ=255)                                                                                                                                                               cGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATg                                                                                  >  1:173715/1‑251 (MQ=255)                                                                                                                                                                gATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGACAAAGCAGGCCAGCTTTATTGTGGAAGGCAATGc                                                                                 >  1:284608/1‑251 (MQ=255)                                                                                                                                                                gATCCGGGATACACGGCAACATTAAAGCATGACCAGCCCTTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAAACGTGCGCGTTTATTAAAACAGTCCCAAAATCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGc                                                                                 >  1:301984/1‑251 (MQ=255)                                                                                                                                                                  ccctggATACATTCCAACATAAAATCATGACCAGCAATTATTGAGTTTGGCCTAGTCTTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCCAAACACATATCTCTTTCAGCTACCCCGCCCTCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAGGCAGGCCAGCTATATTGTTGAAGGCAAAGCCg                                                                               <  2:239530/247‑1 (MQ=255)                                                                                                                                                                      ggATCCACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAg                                                                           <  1:398798/251‑1 (MQ=255)                                                                                                                                                                           cacTGCAACATTAAAGCATGACCAGCCATTCTTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCaaa                                                                       >  2:345697/1‑250 (MQ=255)                                                                                                                                                                               gCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTACATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCTCCCGCCAGCGGATGCCAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGGGGAAGGCAATGCCGAAAGCAAAAggac                                                                  >  2:11502/1‑249 (MQ=255)                                                                                                                                                                                    aTTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTAc                                                             >  2:133078/1‑251 (MQ=255)                                                                                                                                                                                                     cATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCCTTTGCCAGCGCCACCACATCACATATCTCTTTCCGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGTAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCggg                                              >  1:23034/1‑249 (MQ=255)                                                                                                                                                                                                      attattGAGTATGTCCTTGCCGTCGATTACATCCTTTGTATTGTCTGGATTACTGTTGCTTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCACGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATATGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCCGCACAACCGCGAGc                                           >  2:151878/1‑251 (MQ=255)                                                                                                                                                                                                           tGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGAt                                       <  1:214257/250‑1 (MQ=255)                                                                                                                                                                                                                           gacGATTCCATCCTTTGTATTGTCTGGCTTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGACAGCGGATGAAAAACCGTGCGCGTTTATTAAAACAGTACCAAAATCATCTGAAAAAGCAGGCCAGCTCTATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCCGAACAACCTGGAGCAGATAAAACAGCATCCAGAAt                      >  2:401196/3‑251 (MQ=255)                                                                                                                                                                                                                           gTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTCCGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAAt                      >  2:148472/1‑251 (MQ=255)                                                                                                                                                                                                                                   cATCCTTTGTATTGTCTGGATTCCTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCt              <  1:67886/251‑1 (MQ=255)                                                                                                                                                                                                                                          tGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCg        <  1:169830/250‑1 (MQ=255)                                                                                                                                                                                                                                               tGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACCTCACATATCTATTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCTCacg  >  2:370768/1‑249 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       GGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTAAGTTTGTTCAGAAACGATCCGGGATACACTGCAACATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCTCAAG  >  NC_000913/246505‑246993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑