Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,169,8360AG96.8% 92.0 / ‑5.9 31L180P (CTG→CCG) ldtCL,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (13/17);  minor base T (0/1);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCGG  >  NC_000913/1169594‑1170077
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 
gATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGATTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTTATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCTTACCATGAAGCAAATAAACGCCGCCAGAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGc                                                                                                                                                                                                                                            <  1:241329/250‑1 (MQ=255)
                      aCCGTTTGGTTCGGCCGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATCGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCa                                                                                                                                                                                                                      >  1:141789/1‑250 (MQ=255)
                       ccggttGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTTGGGCCTGGGGTGCCCTGGCTAAAGAGTGTTTGGATAGCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTCCGTACCAGGAAGCAAATAACCGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCAGTGGGTTATCCGGTCCAGCCGGCACTACCGCATGCAACTCa                                                                                                                                                                                                                      <  2:381212/245‑1 (MQ=255)
                       ccGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCa                                                                                                                                                                                                                      >  1:381212/1‑249 (MQ=255)
                                                     gtgtTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGtt                                                                                                                                                                                      <  1:83158/251‑1 (MQ=255)
                                                        ttGATGATATTCCCTTTGGTGCCTGGGTGGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATTCAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGGGTTCGTACCATGAAGCAAAGAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCACGGATGTTTg                                                                                                                                                                                    <  2:55909/250‑1 (MQ=255)
                                                           ctgatATTCACTTTGGTGCCTGTGGTGACCTGGATAAAGAGTGTTTTGATATCGTCCTCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGCCATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCCTGATGGCCCATTGGGTTATCCGGGCCAGCCGGCACTACAGCAAGCAACTCCATTCCCTGCGCTTTATAACAGGCGCGGCTGTTTGCCGt                                                                                                                                                                                >  2:289203/2‑251 (MQ=255)
                                                             gatATTCCCTTTGGTGCCTGGGGGGACCGGGCTAAAGAGTGTTTTGATATCGCCACCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGTCACTACCGCAGGCAACTCAAGTCCCTGTGCTTTATAACGGGCGCTGATTTTTTCCGTTg                                                                                                                                                                              <  2:42014/251‑1 (MQ=255)
                                                                   cACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTc                                                                                                                                                                         <  1:132981/250‑1 (MQ=255)
                                                                                                          gattgcgtaaTCCCGCAGACGAATACACCCAGAACTTACCCCCATGCGACTGCAGAAAGCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGTTTCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTGATAACGGGCGCGGATGTTTTCCGTTGGCTACAAGGTTGGGTTTGCACTTTTGTCTGAATCGGTGGTCAcc                                                                                                                                  <  2:350285/241‑1 (MQ=255)
                                                                                                                             cGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTc                                                                                                              >  1:204498/1‑251 (MQ=255)
                                                                                                                                           aCTTACCCTCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCTCCTAACTGAc                                                                                                <  2:141789/251‑1 (MQ=255)
                                                                                                                                             ttACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAAGAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGGCCAGCCGGCACTACCGc                                                                                                                                                                                                                                <  1:259719/121‑1 (MQ=255)
                                                                                                                                             ttACCAGCATGCCAATGCCGAAATCGGCGTTCGTACCATGACGCAAATATCAGCCGCCATAGGCCGCCAGACGC‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGc                                                                                                                                                                                                                                >  2:259719/1‑121 (MQ=255)
                                                                                                                                                      ggcccaTGCCGGAATCCGCTTTCGTGCGATGAAGCAAATGAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGAGGTCCAATTGGGTTATCCTGACCATGCGGCACTACTGCAGGCAACGCAATTCCCTGTGCTTTATAACTGGCGCGGATGTGTGCCGTTGGCGTCAAGGTTGGGTTTGCACGTTTTTCTTAAACGGTGGTCACCATGAGCTGTGTCAGCGTGTCACCACCTAACTGACCAATACCTAtt                                                                                     <  2:161028/246‑1 (MQ=255)
                                                                                                                                                               cGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACg                                                                            <  2:31734/251‑1 (MQ=255)
                                                                                                                                                                     cGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTAcc                                                                      >  1:105826/1‑251 (MQ=255)
                                                                                                                                                                         gTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAAt                                                                  >  2:96703/1‑251 (MQ=255)
                                                                                                                                                                                           aTAAACGACACCATAGGCCGCCAGA‑GACATCACATGAAGGCCCATTGAGTTATCCGGACCAGACGGCACTACCGCAGGCAAATCACTTCTCTGTGCTTTATCACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCAAGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCACTACCTATTGGAAACACGGTTACCGAATTTTTACCCGGCGGgt                                                   >  2:361585/1‑248 (MQ=255)
                                                                                                                                                                                                   cgccATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGTTAGTAATAgaga                                        <  2:204498/251‑1 (MQ=255)
                                                                                                                                                                                                    gccATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGAc                                       <  1:216579/251‑1 (MQ=255)
                                                                                                                                                                                                       aTAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCa                                    <  1:131929/251‑1 (MQ=255)
                                                                                                                                                                                                       aTAGGCAGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGc                                     >  1:94983/1‑250 (MQ=255)
                                                                                                                                                                                                        tAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTc                                                                                                                                     <  1:373255/153‑1 (MQ=255)
                                                                                                                                                                                                        tAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGAGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTc                                                                                                                                     >  2:373255/1‑153 (MQ=255)
                                                                                                                                                                                                                          cGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATc                   <  1:9553/250‑1 (MQ=255)
                                                                                                                                                                                                                            cATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAgaga                                        >  1:376622/1‑227 (MQ=255)
                                                                                                                                                                                                                            cATGATGGCCCATTGGGTTATCCGGGCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAgaga                                        <  2:376622/227‑1 (MQ=255)
                                                                                                                                                                                                                                    cccATTGGGTGATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTGGGGGTTGCACGTTTGTCTGAAACGGTGGTCGCCATTGTCGGTGGCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATTCACGGTTACCGAATTTTTACCCGGCGGTTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTc        <  1:391557/251‑1 (MQ=255)
                                                                                                                                                                                                                                         tGGGTTATCCGGTCCAGCAGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGGCGCAGCTCCGCAATGTTGATCACAATGCCTTcgcgcg   >  1:72896/1‑251 (MQ=255)
                                                                                                                                                                                                                                          ggggtATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTCGTAATAGAGACGCAGCTCCGCAATGTTGATAACACTGCCTTCGCgcgg  >  2:317248/5‑251 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 
GATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGA‑ATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCGG  >  NC_000913/1169594‑1170077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑