Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,156,913	G→A	G46D (GGT→GAT)	ycfH →	putative metal‑dependent hydrolase YcfH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,156,913	0	G	A	100.0%	110.6 / NA	35	G46D (GGT→GAT)	ycfH	putative metal‑dependent hydrolase YcfH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/19);  total (16/19)

CAGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTG  >  NC_000913/1156672‑1157145                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          cAGGCATCAACCGCGAGATTATCAAAACCGATCTTTTACTGCGTATTGAGCATTACCTCCAACCGGGCGTTGTGCTACCGGTACCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGACATCTCGATGGTCTGGATTATGACTCTTTGCATAAGGACGTGGATGCCGTTCTGGCGAAAGCCGCCGCACGCGATGAGTAATTTTGTCTGGCAGTAGCCACAACATTACCGGATTATTTAc                                                                                                                                                                                                                                   >  2:383226/1‑250 (MQ=255)                                   tttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACGGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGAc                                                                                                                                                                                                      <  1:283512/245‑1 (MQ=255)                                   tttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTGAAGAGAGACATCATTTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAAGCTTTGCATAAGGACGTGGATGACGTTCTGGCTAAAGCCTCCGCACGCGATGTTAAATTTTGTCTGGCAGTCGCCACAACATTACCTGATTATTTACATATGCGGGAGCTGGTAGGCGAACGTGACAAcgt                                                                                                                                                                                                 <  1:257679/250‑1 (MQ=255)                                    ttccTGCGTATTGAGCATTACCTGCAACCGGTCGGTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCAGGTTTTTAGGCGACTCACACTGCCAGCTCGATGGTCTGGATGATGAATCTTTGCATAAGGACGTGGATGACGTTCGGGCGAAAGCCGCCGCACGCTATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTGACATAGGCGGGATCTGGTAGGCGAACGTGACAAcgtc                                                                                                                                                                                                <  2:60594/247‑1 (MQ=255)                                     tACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGATACCGGTTCATCATCTTTAAGTGAGCCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGTTCTGGTAGGCGAACGTGACAAcgtcgt                                                                                                                                                                                              >  2:128201/1‑251 (MQ=255)                                             aTTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCtt                                                                                                                                                                                      <  1:126618/251‑1 (MQ=255)                                             aTTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCt                                                                                                                                                                                       <  2:299597/250‑1 (MQ=255)                                                         cTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGCTGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTCTTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATc                                                                                                                                                                          >  1:384960/1‑251 (MQ=255)                                                              aCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATg                                                                                                                                                                                                                              <  1:144286/194‑1 (MQ=255)                                                              aCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATg                                                                                                                                                                                                                              >  2:144286/1‑194 (MQ=255)                                                                     gTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGa                                                                                                                                                              <  1:16051/251‑1 (MQ=255)                                                                                     cTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGACGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGt                                                                                                                                              >  2:212446/1‑251 (MQ=255)                                                                                         tCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGAATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTaga                                                                                                                                           >  2:25012/1‑250 (MQ=255)                                                                                               agagagACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATCAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGATGAttt                                                                                                                                     >  1:200841/1‑250 (MQ=255)                                                                                                      cagcatGTTTTTAGCCGACTCACACTGCCATCTCGATGGTATGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGGAGGCGAACGTGACAACGTCGTAGTTTCGTGTGGCGTGCAGCCTCTCAACCAGATTGATCCCTACGATGTAGAAGATTTACGCCGTc                                                                                                                             <  2:43268/248‑1 (MQ=255)                                                                                                                  agtcgatacactcTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGACTTACGCCGTCTGGCGGCagaag                                                                                                                 <  2:135379/239‑1 (MQ=255)                                                                                                                  aGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCagaag                                                                                                                 <  1:231944/251‑1 (MQ=255)                                                                                                                                         aTGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGAt                                                                                                                                                         <  1:396964/188‑1 (MQ=255)                                                                                                                                         aTGGTATGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGAt                                                                                                                                                         >  2:396964/1‑188 (MQ=255)                                                                                                                                                 gATTATGAATCTTTGCATAAGGACGTGGAGGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGTGTGAAACTGGGCt                                                                                 <  2:306574/251‑1 (MQ=255)                                                                                                                                                  aTTATGAATCTTTGCATACGGACGTGGATGCCGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATATGGTAGGCGAACGTGACACCGTCGTATTTTCTTGTGGCGTGCGTCCACTCCACCAGAATGCTCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAACCCGGGCTg                                                                                >  2:150474/1‑251 (MQ=255)                                                                                                                                                     atgcaTCTTTGCATAAGGACGTGAATGCCGGTCTTGCGAAAGACGGCGCACGCGTTGTGAAATTTTGTATGGCAGTCGCCACAACATTACCGGATTATTTACGTATGCGGGCTCTGGGAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGGATCAACTCAAGCAGCATGATCCCTACGATGTAGGAGATTTACGACGTCTGGCGGCAGAAGAGGGAGGTGGA‑GCGCTGGGTGAACACGGGCTGGat                                                                             >  2:361418/5‑251 (MQ=255)                                                                                                                                                                 aTAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCGGGCTGGATTATTATTACACg                                                                 <  2:169269/251‑1 (MQ=255)                                                                                                                                                                        cGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTATAGCAGTCGCCACAACAATAACGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACACCGTCGAATTTTCTTGTGTCGTGCATCCACTCAACCCGAATGATCCATACGATGTAGAAGAATTAAGCAGTCTGCCGGCAGAAGAGGGCGTTGTT‑GCGTTGGGAGAAACCGGGCTGGATTATTATTACCCGCCGGaa                                                           >  1:361821/1‑250 (MQ=255)                                                                                                                                                                         gTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAAc                                                         <  1:35639/251‑1 (MQ=255)                                                                                                                                                                             aTGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGTGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTaaa                                                     <  1:218992/251‑1 (MQ=255)                                                                                                                                                                                    tCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGCCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACTTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCTGGCTGGATTATTATTACACTCCGGAAACTAAAGTACGt                                               <  2:365487/250‑1 (MQ=255)                                                                                                                                                                                      gggCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAg                                            <  2:362732/250‑1 (MQ=255)                                                                                                                                                                                                  ccgcACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTc                                >  1:128952/1‑251 (MQ=255)                                                                                                                                                                                                                  aTTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCGGGCTgg                                                                               >  1:18913/1‑188 (MQ=255)                                                                                                                                                                                                                  aTTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGGGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGAGTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCGGGCTgg                                                                               <  2:18913/188‑1 (MQ=255)                                                                                                                                                                                                                      tGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTgg            >  1:116092/1‑251 (MQ=255)                                                                                                                                                                                                                          tGGCAGTAGCCACAACATTACCGGATTATTTACATATGCGGCATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATAATCCCTCCGATGTAGAAGATTTTCGACGTCTGGCGGCAAAAGGGGGTGTT‑TATGCGCGGGGTGAAACCGGGCGGGATTATTATTACACGCCGGAAACTAAAGGACGTCAGCAAGAGTCCTTCATCCATAATATCCAGATTggcagt        >  2:207907/1‑247 (MQ=255)                                                                                                                                                                                                                           ggCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCACCCCGACTGATCCCTACGATGTAGAAGCTTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAACCCGGGCTGGATTATTATTAAACGCCGGAAACTCAAGTACGTCAGCCAGAGTCCTTCATCCATCATATCCCGATTGGTCGtg       >  1:57171/1‑251 (MQ=255)                                                                                                                                                                                                                                 cGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGtgaactg  <  1:83607/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          CAGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTA‑GCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTG  >  NC_000913/1156672‑1157145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑