Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,098,620	T→A	V245V (GTT→GTA)	ghrA →	glyoxylate/hydroxypyruvate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,098,620	0	T	A	100.0%	101.4 / NA	32	V245V (GTT→GTA)	ghrA	glyoxylate/hydroxypyruvate reductase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/12);  total (20/12)

CAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGG  >  NC_000913/1098411‑1098856                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             cAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATCCCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGCAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTAcc                                                                                                                                                                                                     >  1:25754/1‑251 (MQ=255)               aCGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTAACGCATACCCCTGACACCGTCGGCATTATTAATCAACACTTAATCGAAAAATTACCGGATGGCGCGTATCTCCTCAAACTGGCGCGTGGAGTTCTTGTTGTGGAAGCTGACCTGATCGCGGCGCTGGATAGCGGCAAAGAAACAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGGCCGc                                                                                                                                                                                       >  2:384397/1‑251 (MQ=255)                            tCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGGCGGCATTATTAATCAAAAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGAGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGCACCCTTACCGCCTGAAAGACCGCTCTGGCAACATcc                                                                                                                                                                          >  2:176602/1‑251 (MQ=255)                                   ttCTGAGCCAATGTCGGGTATTGTTTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACAGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGTAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGTTGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTg                                                                                                                                                                   >  2:305773/1‑251 (MQ=255)                                       gAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACg                                                                                                                                                                >  2:345444/1‑250 (MQ=255)                                               gTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCAGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAAcaccc                                                                                                                                                       >  2:125756/1‑250 (MQ=255)                                                                     aCCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTc                                                                                                                                 <  1:212577/251‑1 (MQ=255)                                                                       cGAATACCTCTGAAACCGCCGGCATTATTAATCATCAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCACACTTGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCTCTGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATATCGCCTCATTTCGTCGAGATTACCCGTctc                                                                                                                               <  2:330308/251‑3 (MQ=255)                                                                              cccTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAg                                                                                                                        <  1:202215/251‑1 (MQ=255)                                                                                   aaaCCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTACGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCtgtg                                                                                                                   >  2:145275/1‑251 (MQ=255)                                                                                     aCCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGCTGGCGCGTATCTCCTCAACCTGGCGAGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGCTACCACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTgg                                                                                                                  >  2:267222/1‑250 (MQ=255)                                                                                        gTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGACGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTCCCGCCTGAAAGTCCGCTCTGGCAACATCCCCGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGt                                                                                                               >  1:266229/1‑250 (MQ=255)                                                                                        gTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGt                                                                                                               >  1:326746/1‑250 (MQ=255)                                                                                           ggCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACAt                                                                                                           <  1:345444/251‑1 (MQ=255)                                                                                                              tACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCa                                                                                         <  1:176602/250‑1 (MQ=255)                                                                                                              gacgcGAAAAAGTACCGGATGGCGCGGAGCGCCTCAACCGGGCGCGTGGTGTTCAGGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTGGGATGTTGTTAATCGGGAACCCTGACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGGGACGATAACACCACATGGCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCGAGCACCATGGCCCa                                                                                         <  2:266229/246‑1 (MQ=255)                                                                                                                  cGAAAAATTACCTGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCg                                                                                    <  1:370406/251‑1 (MQ=255)                                                                                                                  cGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCt                                                                                      <  1:384397/249‑1 (MQ=255)                                                                                                                      aaaTTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATCGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGaaa                                                                                 >  1:71027/1‑250 (MQ=255)                                                                                                                      aaaTTACCGGATGGCGCGTATCTACTCAAACTGGCGCGTGGTGTTAATGTTGTGGAAGATGAACTGCTAGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTACCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGACCAGCTCgcaa                                                                                 >  2:240688/1‑247 (MQ=255)                                                                                                                                         aTCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCATTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAACAGGGGAGAGGGTCTGCggg                                                             >  2:210261/1‑251 (MQ=255)                                                                                                                                            tcctcAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCa                                                           >  1:211050/1‑250 (MQ=255)                                                                                                                                                     tGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCt                                                                  <  2:94215/234‑1 (MQ=255)                                                                                                                                                     tGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCt                                                                  >  1:94215/1‑234 (MQ=255)                                                                                                                                                      ggCGTGTGTTGTTCATGCTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAACGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACAGCCTGAAAGTCCGCGCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCGGTGGAGTACATTTCTCGCACCATTGCACAGCTCGAATATGGGGAGCGGGTCTGCGGGCACGTCGACagcg                                                >  2:327259/1‑251 (MQ=255)                                                                                                                                                        cgcgTGGTGTTCATGTTGTGGAAGATGACCTGGTCGCGGCGCTGGATAGCGGAAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGGACATTTCTCGCACCATTGCCCAGCTCTAAAAAGGGGAGAGGGTCTGTGGTCAAGTCGACCGCGCa                                              <  2:63381/251‑1 (MQ=255)                                                                                                                                                         gcgTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCAc                                             >  1:282119/1‑251 (MQ=255)                                                                                                                                                                gtTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTTGATAACGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTCATCGTGAAACCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACAACACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCATCTCGATAAAGGGGAGAGGGTCTCCGGGCAAGTCGACCGCGCACGCgg                                         >  2:155477/1‑248 (MQ=255)                                                                                                                                                                                  gACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGAt                    >  2:250920/1‑251 (MQ=255)                                                                                                                                                                                      tGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACAGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGAGATTACCCGTCCCGCTGAAGCTGTGGCGTACATTTATCGCACCATTGCCCAGCTCGCAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAGAGAATCAGGATTCCt                >  2:296171/1‑251 (MQ=255)                                                                                                                                                                                          cgcgGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGc              <  2:124675/249‑1 (MQ=255)                                                                                                                                                                                                    gATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCgg  <  1:296171/251‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             CAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGG  >  NC_000913/1098411‑1098856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑