Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	784,677	G→A	A49A (GCC→GCT)	zitB ←	Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	784,677	0	G	A	100.0%	106.2 / NA	31	A49A (GCC→GCT)	zitB	Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/19);  total (12/19)

ATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATT  >  NC_000913/784450‑784921                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     atcatcatGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAAGGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCaga                                                                                                                                                                                                                               <  2:89543/251‑1 (MQ=255)            gccCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCAAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGGGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAaccac                                                                                                                                                                                                                    >  1:95752/1‑251 (MQ=255)                         cgcgGCGGGCGGAAGCGTGCTATGGCCTCCCAGACAATCAAAATGGTAAGTACCACGAGGGCGATGGCAGTCACAAACGCCGCTAGCGTGGTCATGCTGAGCCAACCGATAGTTTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGTCGGGTAGGGCGAAAAGCAGGTCGGCAGTATCGTTAATCCCATGGCCCGGATCAGCCAGCAATGCCAGAGAACCATAAAGAAATCCACCAAGGACTTCTAc                                                                                                                                                                                                       <  2:396474/251‑1 (MQ=255)                                  cGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAAAGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGAGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATaa                                                                                                                                                                                               >  1:232620/1‑250 (MQ=255)                                   ggAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAAc                                                                                                                                                                                             >  1:331894/1‑251 (MQ=255)                                       gCGTTCTATCGCCTCCCAGACAATCAAAATGGTAAGTACCACGAGGGATATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCCGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCga                                                                                                                                                                                         <  2:46181/251‑2 (MQ=255)                                                               cAAAATGGTAATTACCACGAGGGCGCTGGCATTCACAAACGCCGCGAGCGTGGTCCGTCTTAGCCAACCGAAAGTGGGGCGAATGGTGGGAGGACGTCGTGAAAATTGCACGGCGAGTAGGGCGACAAGCAGGGCGGCAGTATCGGTCAACACATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCACCGACTTTTACCATCATAACCCCGGCAGTAACGCCGCAAGCATacaata                                                                                                                                                                 <  2:80535/251‑3 (MQ=255)                                                                 aaaTGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAg                                                                                                                                                               <  1:300290/251‑1 (MQ=255)                                                                       tAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACACCAAGcgacg                                                                                                                                                          >  1:320850/1‑250 (MQ=255)                                                                        aaTTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGCCCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAg                                                                                                                                                        <  1:94247/251‑1 (MQ=255)                                                                        aaTTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGcgacg                                                                                                                                                          >  1:296628/1‑249 (MQ=255)                                                                             ccACGAGGGCTATGGAATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAAACCGGCAGTCACGCCGAAAGCAGACAACAAGCGTCGAGCatta                                                                                                                                                   <  2:66096/251‑1 (MQ=255)                                                                                    ggCTATGGCAGTCACAAACGCCGCGATCGTGGTCAGTCGTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTAGCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTAtctt                                                                                                                                            <  1:142595/251‑1 (MQ=255)                                                                                                                 tGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCCGCATAAACCCGGCAGTCACGACGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGAGTGAGGACGTgtgtgagtgt                                                                                                               >  1:286014/1‑251 (MQ=255)                                                                                                                      aGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGtgagtgtgagtg                                                                                                          <  1:151559/251‑1 (MQ=255)                                                                                                                           tAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTAGCGGTCAACATATGACCCTCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGcc                                                                                                      <  2:168424/250‑1 (MQ=255)                                                                                                                            aGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCAt                                                                                                    >  1:385808/1‑251 (MQ=255)                                                                                                                              ccAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGa                                                                                                  <  1:188686/251‑1 (MQ=255)                                                                                                                                        gtgtGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGAAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCAAGAGAACAAGAAAGAAAACAACCAACGACTTATACCAGCATAAACCCGGCAGTCAAGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTTTGGCAGGTGTGAGGACGTGTGTGAGTGTGCGTGCGCCATGACGAGTCATcc                                                                                        >  1:50274/1‑251 (MQ=255)                                                                                                                                                   ggtggGAGTACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCATCCAGCAATGCCAGAGATCCAGAAATAAAACCACCAACGACTTCTACCAGCATAATCCCGGCATTCACTCCGAAAGCTTACAACAAGCGACGAGCATGATTATCTTCTGGCAGGTGTTAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCAGCCCTTAATTATtc                                                                             <  1:203420/251‑1 (MQ=255)                                                                                                                                                      gggaggacgaagttaaagtgtcgcGGCGAGTAGGGCGAAAAGCCGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGTCAGAGAACCAGAAAGAAAACCACCATCGACTTCTACCAGCATACACCCGGCATGCACGCCGAAAGCATACAACAAGCGACGAGCAGTATTATCTTCGGGCAGGGGTTAGGTCGTGTGTTAGTGTGTGTGCGCCATGACGAGGCATCACTCAATTATACTCa                                                                          <  2:270180/228‑1 (MQ=255)                                                                                                                                                                              ggCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAATCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGc                                                  <  1:33188/251‑1 (MQ=255)                                                                                                                                                                                      gggCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCttttcga                                           >  1:4775/1‑247 (MQ=255)                                                                                                                                                                                               gCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTTTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTGATGACATCATACTGCTTTTAGAAGTGAAAAAt                                 <  2:249297/251‑1 (MQ=255)                                                                                                                                                                                                  gggCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTaa                              <  1:168605/251‑1 (MQ=255)                                                                                                                                                                                                      ggcAGGATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAGAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAgt                          <  2:285624/251‑2 (MQ=255)                                                                                                                                                                                                            aTCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCCGTCACGCCTCAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGAGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGCCATCATTCTGCTTTTAGAAGTGAGAAATTACACGGgag                       >  2:91773/1‑248 (MQ=255)                                                                                                                                                                                                               gggcAACATATGACCCGCATCAACCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCa                                                                                                                                                                                   <  2:350766/86‑1 (MQ=255)                                                                                                                                                                                                               ggTCAACATATGACCCGCATCAACCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTcc                                                                                                                                                                                   >  1:350766/1‑88 (MQ=255)                                                                                                                                                                                                                gTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTcc                >  2:305284/1‑251 (MQ=255)                                                                                                                                                                                                                              cGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCattatt  <  2:331894/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     ATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATT  >  NC_000913/784450‑784921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑