Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	662,269	C→T	D4N (GAT→AAT)	lipB ←	lipoyl(octanoyl) transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,269	0	C	T	100.0%	105.4 / NA	32	D4N (GAT→AAT)	lipB	lipoyl(octanoyl) transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/18);  total (14/18)

CTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTACC  >  NC_000913/662030‑662505                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             cTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACaaa                                                                                                                                                                                                                                    >  1:1123/1‑250 (MQ=255)                       gTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTAGCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAg                                                                                                                                                                                                             <  2:62352/251‑1 (MQ=255)                              gCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTAGTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAg                                                                                                                                                                                                       <  2:240204/250‑1 (MQ=255)                                      cgATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGaaa                                                                                                                                                                                              <  2:376623/251‑1 (MQ=255)                                          cGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAga                                                                                                                                                                                           >  2:302196/1‑249 (MQ=255)                                                             atCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAggg                                                                                                                                                                       >  1:137102/1‑251 (MQ=255)                                                                    ggcattccaaTGAGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCCGCCAGATTTCATCAAGGGTACTATCATCGCGGGAATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATTCAAAACGGCCTCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGAATATCACAGCGAGGAGAGGGGAGGTAc                                                                                                                                                                >  2:21271/9‑251 (MQ=255)                                                                                 gCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAATGGTACTATCATCGCGGGTATCGGTGAAGTCATGCATAGTCTGGGAGATTGGCTCGTAAGGCTGAAGACCGGGCTGTCGGACAAGAATTTTATTCTGATAGAAAACGGCATCTCCGTGGAGAAGGAGTAAAAGCGGGGGAGAAGTATTGCACATCGAGGAGAGGGGAGTGACTCGACCAGGAGcc                                                                                                                                                   <  2:394402/251‑1 (MQ=255)                                                                                   tCCGCTTTTCCAACATGACCTTGGGTGGATAACGGCTAGTGCTAGAAAAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCAGTGAATTTATGCCTAGCCTGGGAGACTGGCTCGAAAGGCTCAAAACCGAGCTGGCGGGCAAGAGTATTATTATGATACAAAACGGCATCTCCGTGGCGGATGAGTAAAATAGGGGGAAAAGGACAGTACAGCGAGGGGAGGGGCGATAACCGACCAGGAGACgg                                                                                                                                                 >  2:248172/1‑251 (MQ=255)                                                                                         tttCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTaa                                                                                                                                             <  2:301759/249‑1 (MQ=255)                                                                                           tCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGGGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACgg                                                                                                                                          >  1:7341/1‑250 (MQ=255)                                                                                            cctgcctgACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGgag                                                                                                                                        <  1:201933/251‑1 (MQ=255)                                                                                                 ctgACATTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGCGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGGAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGAAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCg                                                                                                                                   >  1:328522/1‑251 (MQ=255)                                                                                                    aCCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTGCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGGTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGt                                                                                                                                <  2:174416/251‑1 (MQ=255)                                                                                                                   cGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCgg                                                                                                                 >  1:13378/1‑251 (MQ=255)                                                                                                                              cGACCAGCCAGATTTCAGCAAGGGTACTTTCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGATAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCg                                                                                                        <  1:350095/249‑1 (MQ=255)                                                                                                                                           ttCATCAAGGGTACTATCATCGCGGGTATCGGAGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGGACGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGGGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGGTAACCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGttct                                                                                         >  2:41219/1‑251 (MQ=255)                                                                                                                                                  aGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTAc                                                                                                                                                                >  1:112907/1‑173 (MQ=255)                                                                                                                                                  aGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTAc                                                                                                                                                                <  2:112907/173‑1 (MQ=255)                                                                                                                                                                   ggTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGATAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCg                                                                 <  1:248172/251‑1 (MQ=255)                                                                                                                                                                        cGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGAGGGAAAAGTATATCACAGCGAGGAGGGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGAGTTTCAACCTGCTCGATATg                                                            >  1:25117/1‑251 (MQ=255)                                                                                                                                                                        cGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGGGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATg                                                            >  1:226558/1‑251 (MQ=255)                                                                                                                                                                         ggTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGa                                                           <  2:120287/251‑1 (MQ=255)                                                                                                                                                                            gAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGt                                                         <  1:302196/250‑1 (MQ=255)                                                                                                                                                                              aTTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAAAATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGatattggtggg                                                      >  2:89742/1‑244 (MQ=255)                                                                                                                                                                                tCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTTCTCGATATGAGTGGCg                                                     <  2:183183/250‑1 (MQ=255)                                                                                                                                                                                tCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGGATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCg                                                     <  1:358160/250‑1 (MQ=255)                                                                                                                                                                                      aTAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTtgatag                                              <  2:226558/251‑1 (MQ=255)                                                                                                                                                                                               gagaTTGGCTCATAAGGCTGAACACCTAGCTGGCGGACCAGCGTTTTGTTCTGATACAACAAGGCATCTCCGTGGAGAATGAGAAAACGGGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACAGGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGATTCAACCTGCTCGTTATGAGGGGCGTTGATAGTgatagat                                      >  2:175474/1‑250 (MQ=255)                                                                                                                                                                                                  aTTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCg                                  <  1:41219/251‑1 (MQ=255)                                                                                                                                                                                                            aaGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGt                         <  1:250823/250‑1 (MQ=255)                                                                                                                                                                                                                                  gACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTGGATAGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTAcc  <  1:181863/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             CTGTTGCCCCGGCCCGTGATAAGTCACCTGCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTACC  >  NC_000913/662030‑662505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑