Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	100.0%	140.8 / NA	45	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (25/20);  total (25/20)

GCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGG  >  NC_000913/4636680‑4637161                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             gCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGt                                                                                                                                                                                                                                         <  1:145791/251‑1 (MQ=255)   cTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCCACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGTTTTGCTCTCTGGGGACCCAACGACTGGGCAACTGGCGCAGGCGTTTAATCAGCTCCAACATCCCCCGttt                                                                                                                                                                                                                                       >  1:204844/1‑251 (MQ=255)     gatgggCCTGTAATGCGTAGCGGCATGCGGTTGTTTCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTGTTTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTTCAACATCCCCCGTTTcg                                                                                                                                                                                                                                     <  2:76760/248‑1 (MQ=255)      aGGGGCCTGTAATGCGTATAGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGGTCGTACTGGCCATTTTTGTCAAAGAAGGTAAACCGGGCGTGCGAAGCGCAACGGAGGGGACGTTGATCGACACAGCAACGGTGCTGGCGGAGCTGGCGCGGCACGATTTGCTCTCTGGGGACCCAACGCATGGGCGACTGGGGCCGGCGGTGAATCAGCTGCCACATCCCCCGtgtagc                                                                                                                                                                                                                                    >  2:285311/1‑246 (MQ=255)           ccTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCa                                                                                                                                                                                                                                >  1:362738/1‑250 (MQ=255)            cTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAat                                                                                                                                                                                                                              >  1:13131/1‑251 (MQ=255)              gtaattgttatcgtattgtggTTGTTGTTGGTCTATTTTTTCATTGTGGCGGTGGATGCTTGTTTCGTTCTGTCCATTTTTGTCAAAGAAGTGAAACCGTGCGTGCGAAGTGCAACGGAGGGGACGTTGATCGACACCTCAACGTTGCTGGCGGAGCTGGCGCGTCCCTATTTGCTCTCTGGGGACCCAACGCATTTGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTTGTGCCAat                                                                                                                                                                                                                              <  2:126696/230‑1 (MQ=255)                   gCGTATCGGCATGCGGTTGTTTCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCATCTACAACATCCCCCGTTTCGCGCCAATATCGGt                                                                                                                                                                                                                        <  1:173736/250‑1 (MQ=255)                       aTCGGCATGCTGTTGTTTCTGGGCTATTGTGTACTTGTGGCGTTGGCACGCTGGTTCGTACTGGCCATTTTTTTCAAAGAATTTAAACCGGGCGTGCGAAGGGCAACGGAGGTGACGTTGATCGACACCGTACCGTTGCTGGCGGCGCTGGCGCGTCCCGATTTGCTCTCTGGGGCCCCAACGCAGGGGCAACTGGTGGAGGCGTTTAATCAGCTACAACAGCCCCCGTTTCGCGCCAATATCGGTGGCAt                                                                                                                                                                                                                   <  1:79708/251‑1 (MQ=255)                           gCATGCGGTTGTTGCTGGGCTATTTGTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATAGCGGTGGCATTAAc                                                                                                                                                                                                               <  1:318047/251‑1 (MQ=255)                                  gttgttTCTTGGCTATTTTTGACTGTTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGTCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACACCATCCCCCGTGTCGCGCCAATATCGGTGGCATTAACAAAGttc                                                                                                                                                                                                        <  1:375868/251‑3 (MQ=255)                                    tgttgCTGGGCTATTTTTTACTGGTGGCGGTGGCATCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAAACGGGCGTGCGAAGCGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGGGTCCCGATTTGCTCTCTGGGGACCCAACGCAGGGGCAACTGGCGCAGGCGGTTAATTAGCTACAACATCCCCCCTTTCGCGCCAATATCGGTGGCCTTCAAAAAGTgcgc                                                                                                                                                                                                      >  2:11814/1‑251 (MQ=255)                                    tgttgCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTgcgc                                                                                                                                                                                                      >  1:61286/1‑251 (MQ=255)                                              cTATTTTTTACTGGTGGCGGTGGCAGCCTGGATCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAAAGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGATTCGCGCCAATATAGGTGGCATTAACAAAGAGCGCAATGAatctc                                                                                                                                                                                            >  1:339689/1‑248 (MQ=255)                                                  tttttACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATg                                                                                                                                                                                         <  2:268652/250‑1 (MQ=255)                                                             ggcggtggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGCCGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCCATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCg                                                                                                                                                                             >  1:198391/1‑251 (MQ=255)                                                                 gtggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATgc                                                                                                                                                                         <  1:196746/251‑1 (MQ=255)                                                                 gtggcAGACTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGCATATCATGTCTATATGACCGCTgc                                                                                                                                                                         >  2:159783/1‑251 (MQ=255)                                                                      aGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGCTCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTCACAAAGAGCGAAATGAATATCATGTCTATTTGACCGGTGCGCAgg                                                                                                                                                                    >  2:135163/1‑251 (MQ=255)                                                                           ggTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCCGGGCaa                                                                                                                                                                >  1:101285/1‑250 (MQ=255)                                                                               cGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTAt                                                                                                                                                           <  1:295952/251‑1 (MQ=255)                                                                                  aCTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGt                                                                                                                                                        <  1:159783/251‑1 (MQ=255)                                                                                  aCTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGt                                                                                                                                                        <  1:78585/251‑1 (MQ=255)                                                                                       ccatttgggTCAAAGAAGTGAAACCGGGCGTGCGTAGAGCAACGGAGGGGACGTTGATCGACACCGTAACGTTGTTGGCGGAGCTGGCTAGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCTTTTAATCAGCTATAACATCCCCCGTTTTGCGCCAATATCGTTGGCATTAACAAAGTGCGTAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGAt                                                                                                                                                   <  1:158850/243‑1 (MQ=255)                                                                                                        gTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGt                                                                                                                                  <  1:157985/251‑1 (MQ=255)                                                                                                                                     ggggACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACg                                                                                                     >  1:250621/1‑251 (MQ=255)                                                                                                                                      gggACGGTGAGCGACCCCTCAACGGTGCTGGCGGAGCAGGCGAGTCCCGATTCGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTGTCGCGCCAAGATCGTTGGCATTAACAAAGTGCGCAATGAGTATCATGTCTATATGACCGATGCGCAGGGCAAAGTAGTGTTCGATTCTGCAAATAAAGCCGTTAGACAGTATAATTCGCGCTGGAATGACGt                                                                                                    <  2:177046/251‑1 (MQ=255)                                                                                                                                        gACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCt                                                                                                  >  1:169171/1‑251 (MQ=255)                                                                                                                                            ttGATCGACAAAGCAACGTTGCTGGCGGAGCTAGCGCGTCACGCTTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATAAGCTACAACATCCCCCGTTTCGAGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGAATTGTTCGATTCGGCAAATAAAACCGTTGGACAGGATTATTAGCGCTGGAATGACGTCTGGCt                                                                                              >  2:229879/1‑251 (MQ=255)                                                                                                                                             tGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCt                                                                                              >  1:336364/1‑250 (MQ=255)                                                                                                                                             tGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCt                                                                                              >  1:306085/1‑250 (MQ=255)                                                                                                                                                tCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACg                                                                                          >  1:201290/1‑251 (MQ=255)                                                                                                                                                     acCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCg                                                                                     >  1:235120/1‑251 (MQ=255)                                                                                                                                                            ggtagCTGGCGGAGCTGGGGCATCCCGATTTGGTCTCTGGGGGCCCAAAGCAAGGACAGATGGCGCAGGCGTTGAATCAGCTAGAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATAAGACCGACGCCCAGGGCAAAGTATAGTTGGCGTAGGCAAATAAAGCCATGGGGAAGAGTTATTTGCGCAGGAATGACGTCTGTCTacagttgagaggtc                                                                                >  2:16736/5‑236 (MQ=255)                                                                                                                                                                   ggCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCAGGGGCAACTGGCGCAGGCGTTTAATCAGCGACAACAGCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTGCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTg                                                                       <  1:6388/251‑1 (MQ=255)                                                                                                                                                                      ggAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTgcgc                                                                    >  1:384947/1‑251 (MQ=255)                                                                                                                                                                                cgTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGtt                                                          >  1:5733/1‑251 (MQ=255)                                                                                                                                                                                           gCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGTATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCt                                                <  1:248151/250‑1 (MQ=255)                                                                                                                                                                                                    ggACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCg                                      <  1:193097/251‑1 (MQ=255)                                                                                                                                                                                                     gACCCAACGCATGGGCAACTGGCGCAGGTGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATCTCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAATTAAAGCCGTTGGACAGGATTATTCGCGCTGGCATCAAGTCTGGCTAACGTTGCGTGGTCAGTATTGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCg                                      >  2:143184/1‑250 (MQ=255)                                                                                                                                                                                                             gCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCtg                             >  1:59107/1‑251 (MQ=255)                                                                                                                                                                                                                     aaCTGGCGCAGGCGTTTAATCAGCTCCAACCTCCCCCGTTTCACGCCAATATCGGTGGCATTAACCACGTGCGCCATGAATATCATGCCTATATGACCGATGCGCAGGGCAAAGTCTTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGCTTATTCGCGATGGAATGACGTCTGGCTAACGTTGCGTGCTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGatgtat                     >  2:356003/1‑251 (MQ=255)                                                                                                                                                                                                                           cgcAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATAGCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGcc               <  2:309989/251‑1 (MQ=255)                                                                                                                                                                                                                                    ttAATCAGCTACTACATCCCCAGTTTCGCGCCAATATCGTGGGCATTAACAAAGTGAGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCACAGTATTGTTCGATTCGGCAAATAACGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTATGGCTAACGTTGCGTGTACAGTAGGGTGCGCGCAGCACGTTGCAAACTCATGCCGATCCCGAAAGTTCTGTGATGAATGTTGCCGCACAGAtt      >  2:318524/1‑251 (MQ=255)                                                                                                                                                                                                                                         cAGCTTCAACATCCCCCGTTTCGCGACAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATgg  <  2:116815/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             GCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGG  >  NC_000913/4636680‑4637161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑