Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,587,365	C→T	S139S (AGC→AGT)	mrr →	Type IV methyl‑directed restriction enzyme EcoKMrr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,587,365	0	C	T	97.8%	145.1 / NA	46	S139S (AGC→AGT)	mrr	Type IV methyl‑directed restriction enzyme EcoKMrr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/25);  minor base G (0/1);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ATCGTGCAGGCTGGGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGCCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCA  >  NC_000913/4587118‑4587608                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    agcGTGCAGGCTGGGCGCATGACCGTTGAAAACGTGCCGGGTTGTCGCAAAGGTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTGTTGAGAATGTCAAACTTAAGTCACGGCTGGATTCTGTCGAGTTAGATCCTAAAGCCGACTCTCCCGATCATTAAGAACTGGCAAAGATCAGTCCg                                                                                                                                                                                                                                                  <  2:44266/249‑1 (MQ=255)         ggCTGGGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGATTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCg                                                                                                                                                                                                                                          >  1:236447/1‑251 (MQ=255)             gggCGCATGACCGTTTAAAGCGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTTAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGGCCGGACGATCGGTTa                                                                                                                                                                                                                                      <  1:65616/251‑1 (MQ=255)              ggCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGtt                                                                                                                                                                                                                                       >  1:276632/1‑249 (MQ=255)                  cATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCTGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCa                                                                                                                                                                                                                                 <  2:308666/251‑1 (MQ=255)                          tttAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGCGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTa                                                                                                                                                                                                                          >  1:164551/1‑250 (MQ=255)                               aaCGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGCTCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAag                                                                                                                                                                                                                      >  1:375974/1‑249 (MQ=255)                                aCGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGGACTTGCACAGAGAAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGc                                                                                                                                                                                                                   >  2:106233/1‑251 (MQ=255)                                              cGCAAAGTTTGTCGCGTGGAAAATGGTGCCTGACTCCTGCGTGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGGGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGtt                                                                                                                                                                                                                                       <  1:354571/217‑1 (MQ=255)                                               gcaaatgttGTCTCGTGGCAAATGGTGCCTGACGCCTGCGGGGTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGt                                                                                                                                                                                                              <  2:353089/234‑1 (MQ=255)                                               gCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGt                                                                                                                                                                                                              >  1:353089/1‑241 (MQ=255)                                                       tGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTACCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTGCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGAGGCCGTCGAGTTAGATCCGAAAGCCTACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGGTCGTTTAGATCAGGCGGTAAAAGAGCGACGTGATGCGGTGGCagatta                                                                                                                                                                                             <  2:397404/250‑3 (MQ=255)                                                             gTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTg                                                                                                                                                                                      <  1:269511/251‑1 (MQ=255)                                                              tGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGTTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCt                                                                                                                                                                                       >  2:95442/1‑249 (MQ=255)                                                              tGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCt                                                                                                                                                                                       <  1:95442/249‑1 (MQ=255)                                                               ggccaaTGGTGCCTGACTCCTGCGGGTTTTGACGGGGTTGCGGCTCAGCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGGCGATTTAGATCCGAAAGCCGACTCTCCCGATCACGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGa                                                                                                                                                                                    <  2:272237/247‑1 (MQ=255)                                                                 cAAAAGGTGCCTGACGCCTGCGGGGTTTGACTGTGTTGCGGCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCGTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGAGCCGAAAGCCGACTCTCCCGATCATGAATAACGTGCAAAGAGCAGTCCTGACGAACGGTGAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGTTTCTGGaaa                                                                                                                                                                                  <  2:191825/251‑1 (MQ=255)                                                                           cTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGc                                                                                                                                                                         >  1:124280/1‑250 (MQ=255)                                                                            tGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGc                                                                                                                                                                         >  1:178006/1‑249 (MQ=255)                                                                                    gctggTTTTGGCTGGGTTGCGTCTCAGCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGGCTCTCCCGATCAGGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCATCGTGAGGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTcc                                                                                                                                                               <  1:38711/248‑1 (MQ=255)                                                                                    gCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTcc                                                                                                                                                               <  1:136670/251‑1 (MQ=255)                                                                                       ggTTTTCACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGAAGGAGACGAACCCTCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCAAGGCCGGATGCCGTCGATTTAGCTCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGCTCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTc                                                                                                                                                            >  2:54465/1‑251 (MQ=255)                                                                                        gatttcaCTGGGTTGCGTCTCCTCCACAACCAATGACGGAGAAGGAGACGAACCATCTGGCCTTCGATTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGCAAGCAGCCTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGGTCGGTTAGATCAGACGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGATTCTGGAAAACTTATTGTAGGTTTCTCCTTcg                                                                                                                                                           >  2:374784/7‑251 (MQ=255)                                                                                         ttttGACTGGGTTGCGTCTCATCCCCAGCCACTGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGCTTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTAGTGATGCGGTGGCTGATGGGGTTCTGGAAAACTTATTGCAGGGTTCTCCTTcgg                                                                                                                                                          >  2:132633/1‑250 (MQ=255)                                                                                                        ggctcATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTGCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCGTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACGTATGGCAGGTGGCTCCTTCGCGCTTTGAAGTCATTg                                                                                                                                           <  2:31887/249‑1 (MQ=255)                                                                                                                cccAGCCAATGACGGAGCAGGAGACGAACCATCTGGACTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGTCTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGAGCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCGCCTTCGCGCTTTGAAGTCATTGTTCTGGAt                                                                                                                                   <  2:375974/251‑1 (MQ=255)                                                                                                                  cAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGt                                                                                                                                 >  1:63988/1‑251 (MQ=255)                                                                                                                                       aCGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATgg                                                                                                            <  1:404130/251‑1 (MQ=255)                                                                                                                                         gAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATggcg                                                                                                          >  1:281595/1‑251 (MQ=255)                                                                                                                                                  ggCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGtg                                                                                                 <  2:124280/251‑1 (MQ=255)                                                                                                                                                              tgtgAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAg                                                                                      <  1:6627/250‑1 (MQ=255)                                                                                                                                                                  aaTGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGTAGCgtgt                                                                                 >  1:55912/1‑251 (MQ=255)                                                                                                                                                                         aaCTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGt                                                                          <  2:142034/251‑1 (MQ=255)                                                                                                                                                                                       ccGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGCACTTGCAAAGAGCAGTCCGGACGCTCGGTTAGATCAGGCGCTACAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTgggggtggggg                                                            >  1:381197/1‑242 (MQ=255)                                                                                                                                                                                           aTGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGAACGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTTGCGGTACTGGAGATGGTTGCATc                                                        <  2:192486/251‑1 (MQ=255)                                                                                                                                                                                               cGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATg                                                    <  1:367225/251‑1 (MQ=255)                                                                                                                                                                                                   gATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCCAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCCCCGTGATTATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGgtgg                                                >  1:38811/1‑250 (MQ=255)                                                                                                                                                                                                   gATTTAGATCCGAAAGCCGACTCTCCCGACCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCAGCGATGgtgt                                                <  2:63988/251‑1 (MQ=255)                                                                                                                                                                                                                   ccGACTCTCCCGATCATGACGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTg                                                                                                                             >  1:105013/1‑158 (MQ=255)                                                                                                                                                                                                                   ccGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTg                                                                                                                             <  2:105013/158‑1 (MQ=255)                                                                                                                                                                                                                    cGACTCTCCCGATCATGAAGAACGTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGACGTCGTTGTTCTGGATGTTTTGCATCGCCTGGTGTATGGCGGCCACCGTGATGATTTGCATCGTGTTGGCGGTACTGGTGATGGTGGCATCGATTGTGTGATATCGCTTGATaatc                               <  2:207006/251‑3 (MQ=255)                                                                                                                                                                                                                       ctctCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTTCAGCGTGTTGGCGGTACTGGGGATGGTGGCATCGGTGGTGGGATATCGCTTGATAAACTTg                            >  1:288068/1‑251 (MQ=255)                                                                                                                                                                                                                            ccGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGGTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGTAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTg                       <  1:379225/251‑1 (MQ=255)                                                                                                                                                                                                                                  aTGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGaaa                 <  2:55912/251‑1 (MQ=255)                                                                                                                                                                                                                                            tGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGTCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGt         <  1:199226/249‑1 (MQ=255)                                                                                                                                                                                                                                                  gagCAGTCCGGACGATCGGTTAGATCCGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGCTTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGCTGGTGTGGTATCGCTTGGTAAACTTGGCCTGGGGGAAGGTTATGTTCCGGCa  >  2:182437/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    ATCGTGCAGGCTGGGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGCCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGCA  >  NC_000913/4587118‑4587608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑