Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,564,385	C→T	S102N (AGC→AAC)	yjiL ←	putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehdratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,564,385	0	C	T	98.1%	163.0 / NA	52	S102N (AGC→AAC)	yjiL	putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehdratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/30);  minor base G (0/1);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCC  >  NC_000913/4564139‑4564626                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  ccTGCGAGAATCGCTGCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACGCTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACTCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCTCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTtgttgt                                                                                                                                                                                                                                                <  1:374643/250‑1 (MQ=255)    gCGAGAATCGCTTCTGGCGCGCCGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCt                                                                                                                                                                                                                                             <  1:151311/250‑1 (MQ=255)                       cgtcGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGTTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCTTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTAcc                                                                                                                                                                                                                         <  2:234833/248‑1 (MQ=255)                                cTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTACTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGtt                                                                                                                                                                                                                >  1:197181/1‑251 (MQ=255)                                           cTGATCGCTTCTGATTCAGCCAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCCCAGGTTACCGTCATCATCCAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGAtt                                                                                                                                                                                                                            >  2:66955/1‑228 (MQ=255)                                           cTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGAtt                                                                                                                                                                                                                            <  1:66955/228‑1 (MQ=255)                                                   ttCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCCTGCCATTTTCGGTAATGCTGTCGCGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGCCGCAAGAAACCGTg                                                                                                                                                                                             >  1:181131/1‑251 (MQ=255)                                                       gATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGcc                                                                                                                                                                                         >  1:377523/1‑251 (MQ=255)                                                          tCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCa                                                                                                                                                                                       <  1:212335/250‑1 (MQ=255)                                                               aaaCACTGAGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGt                                                                                                                                                                                 >  2:35254/1‑251 (MQ=255)                                                                    cTGTGAACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGATGTCGAGTTGCTCGACGCTGGTGCCAAGAGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCAAAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCgcgcc                                                                                                                                                                              >  2:339140/1‑245 (MQ=255)                                                                      gtgCACATATTCGTGATGGCGGGCGGCGTGGCATTGGCGGTAATGCTGTCGAGTGGCTCGACGCTGGTGCCAAGCGGGCGCGAGATCTCCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCAGCATCAAGCTGGATCTCGTTGTTGTCCTGCCCTCCGATGTGGATTGCCGTGCGCGGGGCTTTCCCAATTAACCGTGCGCCCAGCCCGTTACAGga                                                                                                                                                                          <  2:267634/251‑1 (MQ=255)                                                                                 cGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGCAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCCCCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGttt                                                                                                                                                               >  2:129522/1‑250 (MQ=255)                                                                                    gATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCTCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTAcc                                                                                                                                                             <  1:25589/250‑1 (MQ=255)                                                                                    gATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGAGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGCCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACAGATGTCGATTAACGCGCGCGTTGCTGGCGCAAGAACCCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCt                                                                                                                                                            >  1:194189/1‑251 (MQ=255)                                                                                       gtcGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCCCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGtt                                                                                                                                                         <  2:92821/249‑1 (MQ=255)                                                                                             cggcgTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCgg                                                                                                                                                   >  1:120166/1‑251 (MQ=255)                                                                                                cgTGACATTTGCGGTAATGCTGTCGCGATGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCaa                                                                                                                                                <  1:345302/251‑1 (MQ=255)                                                                                                    aCATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATc                                                                                                                                            >  2:246586/1‑251 (MQ=255)                                                                                                     cATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATcc                                                                                                                                           <  1:94155/251‑1 (MQ=255)                                                                                                     cATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATcc                                                                                                                                           >  1:249314/1‑251 (MQ=255)                                                                                                                aTGCTGTCGAGTTGCTCGACGCGGGTGCCAAGCGTGCGCGAGATCCCCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTgccg                                                                                                                                <  2:295779/251‑1 (MQ=255)                                                                                                                  gCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCGCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAAGCGCACAGTTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATTTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCTCGTGACAGGAGATTTCCGTTACCTGTTTAACGGCAAAATCCACCAGTTgccgc                                                                                                                               <  2:365502/250‑1 (MQ=255)                                                                                                                     gTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTGCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGccc                                                                                                                             <  2:249314/249‑1 (MQ=255)                                                                                                                               tCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTg                                                                                                                   >  2:111451/1‑249 (MQ=255)                                                                                                                                    gCTGGTGCCAAGCTTGCGCGAGATCACCGCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACGTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTTAGCGTCa                                                                                                            <  1:16136/251‑1 (MQ=255)                                                                                                                                     cTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGa                                                                                                                  >  1:263726/1‑244 (MQ=255)                                                                                                                                     cTGGTGCCAAGCGGGCGCGAGTTCACCCCCAGGAAACGCCCGTTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAAGCCACCAGTTGCCACCCGTAGCCGGTGa                                                                                                                  <  2:263726/244‑1 (MQ=255)                                                                                                                                       ggTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGGTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGaa                                                                                                         <  2:317522/251‑1 (MQ=255)                                                                                                                                           ccAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACgg                                                                                                     >  1:244710/1‑251 (MQ=255)                                                                                                                                               gCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGtt                                                                                                 <  1:100068/251‑1 (MQ=255)                                                                                                                                                 gTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGt                                                                                               >  2:11200/1‑251 (MQ=255)                                                                                                                                                                cccAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTcgcg                                                                                <  2:86713/250‑1 (MQ=255)                                                                                                                                                                   aGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGGCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCTCCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCag                                                                             <  2:391945/251‑1 (MQ=255)                                                                                                                                                                             cGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAg                                                                   <  1:300611/251‑1 (MQ=255)                                                                                                                                                                               gTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGt                                                                                                                           >  2:88547/1‑193 (MQ=255)                                                                                                                                                                               gTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGt                                                                                                                           <  1:88547/193‑1 (MQ=255)                                                                                                                                                                                      ccgcGTATTTGTCAGTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCGCGTTGTTGTCCGGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAGCCGTGCGCACAGCCCGTGACAGGAGATTTCCGTTTCCTGTTGATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTCCGCTCAGAGTTGCCCAGGCTTCAGTa                                                          <  2:79402/251‑1 (MQ=255)                                                                                                                                                                                          gcATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTg                                                      >  2:114782/1‑251 (MQ=255)                                                                                                                                                                                          gcATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTg                                                      >  1:214459/1‑251 (MQ=255)                                                                                                                                                                                                  cattcatCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGATGCCGCACGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGcc                                              >  2:154873/1‑251 (MQ=255)                                                                                                                                                                                                          aGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAAGCACTTTGTTGGCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCTCCCGTAGCCGGTGAGCGTCAGAAACGGCGGTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGaaa                                      <  2:384977/251‑1 (MQ=255)                                                                                                                                                                                                            gAAATCGCACAGGTTTCCGGCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTGGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCTCCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTGCGCGCAGAGTTGCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAgg                                    <  2:244710/251‑1 (MQ=255)                                                                                                                                                                                                               aTCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGGCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAggggg                                 <  1:192375/251‑1 (MQ=255)                                                                                                                                                                                                                 cGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGTTGTCCGGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGtt                               <  2:341466/251‑1 (MQ=255)                                                                                                                                                                                                                   cacaGGTTACCGTCATCATCAAGCTGAATCACTGTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGGCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTgg                             <  2:365304/251‑1 (MQ=255)                                                                                                                                                                                                                      aGGTTACCGTCATCATCAAGCGGAATCACTTTGTTGTCCTGACCCCCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGGCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGTGCGAAAGGGGGTTGGAAc                          <  1:273257/251‑1 (MQ=255)                                                                                                                                                                                                                           aCCGTCATCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATGTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGa                     <  1:129522/251‑1 (MQ=255)                                                                                                                                                                                                                              gTCAGCATCAAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCTCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGGTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCTAAAGGGGGTTGGAACGAGGAAAc                  <  2:251833/251‑1 (MQ=255)                                                                                                                                                                                                                                      caAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGtt                                                                                                 >  2:372207/1‑164 (MQ=255)                                                                                                                                                                                                                                      caAGCTGAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGtt                                                                                                 <  1:372207/164‑1 (MQ=255)                                                                                                                                                                                                                                            gAATCACTTTGTTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCa      <  1:80832/249‑1 (MQ=255)                                                                                                                                                                                                                                              aTCACTTTATTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGcc  >  2:77447/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  CCTGCGAGAATCGCTTCTGGCGCGACGCCCGCTGAGCGCAGGCTGATCGCTTCTGATTCAGCAAACACTGTGCACATACTCGTGATGGCGTGCGGCGTGACATTTTCGGTAATGCTGTCGAGTTGCTCGACGCTGGTGCCAAGCGTGCGCGAGATCACCTCCAGGAAACGCCCGGTGCCCGCCGCGCATTTGTCATTCATCAGGAAATCGCACAGGTTACCGTCATCATCAAGCTGAATCACTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCC  >  NC_000913/4564139‑4564626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑