Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,541,261	C→T	A102V (GCT→GTT)	fimB →	Type 1 fimbriae regulatory protein FimB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,541,261	0	C	T	95.5%	60.5 / NA	23	A102V (GCT→GTT)	fimB	Type 1 fimbriae regulatory protein FimB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (12/9);  minor base G (0/1);  total (13/10)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTAAAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCA  >  NC_000913/4541031‑4541499                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                aaaTACCGGGCCTCATCCAGCACGTACTTATCGTCTGGCTCTGCTTTGTTTTATTCCTGGTTTCCGGACGCGTGAAATTTGTCGATTGAGGATTTCGGATTTTGATCTTAAGGCAAAGTGTATATATCTCCAACGATTAAAAACAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAATTCAGGCTTTAAAAAACTGGTTGAGTTTCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTCtttt                                                                                                                                                                                                                             >  2:151410/1‑251 (MQ=255)                    gcaCGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTAAAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATcc                                                                                                                                                                                                          >  1:39511/1‑251 (MQ=255)                                                 ttttATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTAAAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCAtatt                                                                                                                                                                              >  1:143253/1‑250 (MQ=255)                                                    tATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTAAAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTAtct                                                                                                                                                                          >  2:4887/1‑251 (MQ=255)                                                                                                                    aaGTGTATATATATCCATCGATTAAAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCgtg                                                                                                         <  2:108696/251‑1 (MQ=255)                                                                                                                                   cATCGATTAAAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCGTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATGCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGc                                                                                          <  2:235033/251‑1 (MQ=255)                                                                                                                                      cGATTAAAAAAAGGCTTTTCAAAAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAAAAGTTTTACCATATTATCTCGCCTTCCGGTGG‑AATGGCCGGGTTGTCACTGGAGTTTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGTTTTgggga                                                                                        >  1:113487/1‑247 (MQ=255)                                                                                                                                        aTTAAAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGGTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACGTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAata                                                                                     <  2:209404/251‑1 (MQ=255)                                                                                                                                          tAAAAATAGGCTTTGCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAATCTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTAGCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTGGCTTTGGCTAatat                                                                                    <  2:357152/250‑1 (MQ=255)                                                                                                                                                      tttCAACAAAGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATAcg                                                                       >  1:339793/1‑251 (MQ=255)                                                                                                                                                          tacaacGCACCCGATATTGCATAAAGAAGCTCAGGCTTTAAAAAACTCGTTGAGTATACGTACTTAGAACCCGACTGTTGAGAGCGAGTGGGTATTTTTATCACGTACGAGGAATCCGCTTTCTCGGCAACCGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGAGAATATGGGACTAGATACGCGAc                                                                   >  2:172318/2‑251 (MQ=255)                                                                                                                                                             aacGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACtt                                                                 >  1:93524/1‑250 (MQ=255)                                                                                                                                                                         aTTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCTACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGGGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATc                                                    >  2:378916/1‑251 (MQ=255)                                                                                                                                                                           tGAATCAAGAAGTTCAGGCTTTAAAACACTGGTTGAGCATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGCGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTAtatt                                                  >  2:182097/1‑248 (MQ=255)                                                                                                                                                                            gAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGATATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGTAATAGATACGCGACTTATCCAGGATTATCtt                                                  <  1:227435/250‑1 (MQ=255)                                                                                                                                                                            gAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTg                                                 <  2:176410/251‑1 (MQ=255)                                                                                                                                                                              aTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCggg                                                                                                                                                  <  2:142931/152‑1 (MQ=255)                                                                                                                                                                              aTAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCggg                                                                                                                                                  >  1:142931/1‑152 (MQ=255)                                                                                                                                                                                                    aaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTg                         >  1:210723/1‑251 (MQ=255)                                                                                                                                                                                                         ggTTGAGTATCCGTACTTCGTACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCt                      <  1:357531/249‑1 (MQ=255)                                                                                                                                                                                                              aGTATCCGTACTTCGAACCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGTATAc               >  2:78349/1‑251 (MQ=255)                                                                                                                                                                                                              aGTATCCGTACTGCGTACCCGCATGGTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCGCGACTTCCGGTGGTAATG‑CCTGGTTGTCACTGGAGATTCATCCGCACATGTGACGCCAGTCGTGGGGTTTGGCTTTGGCGAATATGGTAATAGATTCGCGACGTTTCCAGGTTTAGCTTGGGCATCGCAATATTCGTCAGACTGTCTGTTATAc               <  2:62364/251‑1 (MQ=255)                                                                                                                                                                                                                           cggaCCCGCATGTTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCa  <  2:143253/248‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                AAATACCGGGCCTCATGCAGCACGTAATTATTGTCTGACTTTGCTTTGTTTTATTCATGGTTTCCGGGCGAGTGAAATTTGTCGATTGAGGATTTCGGATATTGATCTTAAGGCAAAGTGTATATATATCCATCGATTAAAAAAAGGCTTTTCAACAACGCACCCGCTATTGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATG‑CCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGTATACCGCCAGCAATGCA  >  NC_000913/4541031‑4541499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑