Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,494,499	C→T	intergenic (‑93/‑124)	idnD ← / → idnK	L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,494,499	0	C	T	100.0%	82.3 / NA	26	intergenic (‑93/‑124)	idnD/idnK	L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/18);  total (8/18)

TTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCCA  >  NC_000913/4494255‑4494743                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     tttCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACt                                                                                                                                                                                                                                                >  1:4448/1‑251 (MQ=255)    ccttcctGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATg                                                                                                                                                                                                                                             <  1:246364/251‑1 (MQ=255)               ataatGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAAc                                                                                                                                                                                                                                  <  1:114903/251‑1 (MQ=255)                      aaaTCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCTGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGAtg                                                                                                                                                                                                                            <  2:251152/250‑1 (MQ=255)                                        ccACCTCGGGTTATTTGTACTAATGTTTCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGAGCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATc                                                                                                                                                                                                          <  2:134748/250‑1 (MQ=255)                                        ccACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATc                                                                                                                                                                                                          <  1:327290/250‑1 (MQ=255)                                            cTCGGGTTATTTGTACTAATGTTACATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGCTTTATTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGt                                                                                                                                                                                                       >  2:366158/1‑249 (MQ=255)                                                       tGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTAGTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaa                                                                                                                                                                                           <  1:251206/250‑1 (MQ=255)                                                                   ccATTATTATTCCAATCTATCGGCTGCGCGGTTACGGCAACAGTTTCCTTGCCGGCAACAACGCAGGACTGTGTTTTCACTTTCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTGTTAACTGTCATTATTTGTGATGAAGATCACGTCATAAAATTGTTATATTACTATGTTACGCATAACGTGATGGTCCTTGTAATTCTTATCAGTTGAAATAAAAAATCGTGAAattatt                                                                                                                                                                              <  2:225662/251‑1 (MQ=255)                                                                             tCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCa                                                                                                                                                                     <  2:321419/250‑1 (MQ=255)                                                                             tCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATAGTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCa                                                                                                                                                                     <  2:339020/250‑1 (MQ=255)                                                                                  tCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAAAGAAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGCAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGCAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTCTCAGTAGAAATAAAAAAACGTGAAATTCTTATGCCGCCAGGCgt                                                                                                                                                                >  1:375378/1‑250 (MQ=255)                                                                                       cGTCTGCTCGGTAACGGAAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATc                                                                                                                                                          >  2:279814/1‑251 (MQ=255)                                                                                                   atcGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTaa                                                                                                                                               <  2:141498/248‑1 (MQ=255)                                                                                                        cAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAACTTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAgatgat                                                                                                                                         >  1:368778/1‑251 (MQ=255)                                                                                                                    tGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCGCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGAtt                                                                                                                               <  1:323216/249‑1 (MQ=255)                                                                                                                                               tCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCAGTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTg                                                                                                   <  1:137282/250‑1 (MQ=255)                                                                                                                                                      cATAAATTCCTCACTGGTCAGGTAGACACCTCGGACGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGt                                                                                           >  1:283729/1‑251 (MQ=255)                                                                                                                                                       aTAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGtt                                                                                          <  2:197250/251‑1 (MQ=255)                                                                                                                                                                     ggTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTa                                                                             >  1:35266/1‑250 (MQ=255)                                                                                                                                                                                                     gTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCAGAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATTATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGCGAGTGGAAAAGCATTAATTGGTAGCAAGGTTGCCGCGttaa                                            <  2:283030/251‑2 (MQ=255)                                                                                                                                                                                                          aaCTGTCATTGTATGTGATGAAGATCCCGGCAGAAAATTGTTATATTACTATGTTAAGCATAACGAGATGTGCCTTGTAATTCTTATCAGAAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGAGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCt                                       >  2:176718/1‑251 (MQ=255)                                                                                                                                                                                                                                   cACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAAGTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAAGGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTAGTATCTGCTAAATTGAGTGATGGGGACGAt               <  2:368778/250‑1 (MQ=255)                                                                                                                                                                                                                                        cAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCAt         <  1:62561/251‑1 (MQ=255)                                                                                                                                                                                                                                            aaaTTGTTATAATACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAg     <  1:142305/251‑1 (MQ=255)                                                                                                                                                                                                                                                tGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGTTGGGGACGATCTTCATCCAGCCa  <  2:253592/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     TTTCCTTCCTGATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCCA  >  NC_000913/4494255‑4494743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑